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The map label for this gene is lacE2 [H]

Identifier: 221231893

GI number: 221231893

Start: 1065429

End: 1067123

Strand: Reverse

Name: lacE2 [H]

Synonym: SPN23F_10860

Alternate gene names: 221231893

Gene position: 1067123-1065429 (Counterclockwise)

Preceding gene: 221231894

Following gene: 221231892

Centisome position: 48.04

GC content: 35.63

Gene sequence:

>1695_bases
ATGGATAAGTTAATAATTTTTATTGAGAAAGGAAAGCCTTTCTTTGAGAAATTATCGAGAAATATATACTTAAGAGCGAT
AAAAGATGGTTTTATTTCCAGTATGCCAGCAGTATTATTCTCAAGTATTTTTATACTAATAGCTGCTGTGCCCAATATTT
TTGGGTTTAAATGGTCTGATGAACAGTTAGCTTTTATTTTGAAACCTTACAACTACTCAATGGGGATTTTAGCTCTCTTA
GTAGCTGGGACAACTGCAAAATCGTTAACAGATTCAGTTAATACACGTAGTATGGAGAAAACCAACCAAATCAACTACAT
GTCAACTTTTTTAGCAGCTGTTGTTGGATTGTTAATACTGGCAGCTGATCCAATTGAAGGTGGATTTGCTAATGGTCTTT
TAGGTACTAGAGGATTACTTACTGCATTTTTGGCAGCTTTTATTACAGTAAATATCTATAAAGTATGTATTAAAAATAAT
GTAACCATTAGACTTCCTGAAGAGGTTCCACCAAATATTGCACAAGTTTTTAAAGATGTTATTCCGTTTGCCTTATCTGT
TCTTTCAATATATGGATTGGATTTAATTGTTAGAAATATTTTTGGTACAAATGTAGCAGAATCTGTTGGTAAAATATTAG
CCCCTCTATTTTCAGCTACTGATGGTTATATCGGTTTAGCTATTGTATTTGGTGCTTATGCTTTCTTTTGGTTTGTTGGA
ATTCATGGGCCATCTGTTGTAGAACCTTTAATTGTAGCTATCAGTTATGCAAATATAGAAGCTAATGTTCAGCTTGTGCA
AGCTGGTATGCACGCAGATAAAATTTTAAATCCAGTTACTCAAACTTTTGTTGTAACAATGGGTGGTACTGGTGCAACAC
TAGTAGTTCCATTTATGTTTATGTGGTTATGTAAATCAAAAAGAAATAGAATTGTCGGACGTGCGTCTGTAGTTCCTACT
TTCTTTGGCGTAAATGAACCAATCCTATTTGGTGCTCCAATTGTTTTAAATCCTATCTTCTTTATTCCGTTTGTTACTGC
TCCTATTATCAATGTTTGGATTATGAAATTTTTTGTAGATGTTCTACAAATGAATAGTTTCAGTATTATTTTACCTTGGA
CAACACCTGCTCCAATTGGTATTGTTATGGGAACGGCGCTGGCTCCATTGTCATTCGTCTTGGCTATAACTTTAATCATT
ATCGACACTTTAATCTATTATCCATTTGTTAAAGTTTATGACCATCAAATTTTAGAAGAAGAACGGAAAGGAAACTCTTC
ATCTGAATTGAAAGAAAAAGTTGCTGCAAACTTCAACACTGCAAAAGCGGATGCTATTCTTGAAAAAGCGGGTGTCGATG
CAGCACAAAATACCATCACTGAAGAAACAAATGTCCTCGTTCTCTGTGCTGGTGGAGGTACAAGTGGACTCCTTGCAAAT
GCTTTGAATAAGGCAGCAGCAGAATACAATGTCCCTGTGAAAGCAGCAGCAGGCGGCTATGGTGCTCACCGTGAAATGTT
ACCAGAGTTTAATCTAGTTATCCTTGCCCCTCAAGTTGCTTCAAACTTTGAAGATATGAAGGCAGAAACAGATAAGCTCG
GTATTAAACTTGCTAAGACAGAAGGCGCTCAATACATCAAATTAACTCGTGATGGAAAAGGTGCCCTAGCATTCGTACAA
GAGCAATTCGATTAA

Upstream 100 bases:

>100_bases
GTGACCATGATGCATGGTCAAGATCACTTGATGACAACTATCTTGTTAAAAGACCTAATGCACCATTTAATTGAACTTTA
CAAGAGAGGAGTTAAATAAA

Downstream 100 bases:

>100_bases
GGCTAGGAACTGTGAAACAGTCTCCCATCGTTACGGAAATCGCTATGGCGAATTTCCTAATCGCCTTATTAATTCGTCGG
TAAAAAGATATCGTTTTTAC

Product: lactose-specific phosphotransferase system (PTS), IIBC component 2

Products: NA

Alternate protein names: EIICB-Lac; EII-Lac; Lactose permease IIC component; PTS system lactose-specific EIIC component; Lactose-specific phosphotransferase enzyme IIB component; PTS system lactose-specific EIIB component [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIFILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALL
VAGTTAKSLTDSVNTRSMEKTNQINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFSATDGYIGLAIVFGAYAFFWFVG
IHGPSVVEPLIVAISYANIEANVQLVQAGMHADKILNPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPT
FFGVNEPILFGAPIVLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSFVLAITLII
IDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLAN
ALNKAAAEYNVPVKAAAGGYGAHREMLPEFNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQ
EQFD

Sequences:

>Translated_564_residues
MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIFILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALL
VAGTTAKSLTDSVNTRSMEKTNQINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFSATDGYIGLAIVFGAYAFFWFVG
IHGPSVVEPLIVAISYANIEANVQLVQAGMHADKILNPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPT
FFGVNEPILFGAPIVLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSFVLAITLII
IDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLAN
ALNKAAAEYNVPVKAAAGGYGAHREMLPEFNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQ
EQFD
>Mature_564_residues
MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIFILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALL
VAGTTAKSLTDSVNTRSMEKTNQINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFSATDGYIGLAIVFGAYAFFWFVG
IHGPSVVEPLIVAISYANIEANVQLVQAGMHADKILNPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPT
FFGVNEPILFGAPIVLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSFVLAITLII
IDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLAN
ALNKAAAEYNVPVKAAAGGYGAHREMLPEFNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQ
EQFD

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=468, Percent_Identity=25.4273504273504, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014350
- InterPro:   IPR003501
- InterPro:   IPR013012
- InterPro:   IPR003352
- InterPro:   IPR004501
- InterPro:   IPR004801 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61141; Mature: 61141

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS51100 PTS_EIIB_TYPE_3 ; PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIFILIAAVPNIFGFKWSD
CCCEEEEEECCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTNQINYMSTFLAAVVGLLIL
CCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNNVTIRLPEEVPPNIAQVFKDV
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
IPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFSATDGYIGLAIVFGAYAFFWFVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IHGPSVVEPLIVAISYANIEANVQLVQAGMHADKILNPVTQTFVVTMGGTGATLVVPFMF
CCCCHHHHHHHHHHEECCCCCCHHHHHHCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHH
MWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPIVLNPIFFIPFVTAPIINVWIMKFFVD
HHHHHHHHCCEEECHHHCHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQMNSFSIILPWTTPAPIGIVMGTALAPLSFVLAITLIIIDTLIYYPFVKVYDHQILEE
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERKGNSSSELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLAN
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCHHHHHH
ALNKAAAEYNVPVKAAAGGYGAHREMLPEFNLVILAPQVASNFEDMKAETDKLGIKLAKT
HHHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEECCHHHCCHHHHHHHHHHHCEEEEEC
EGAQYIKLTRDGKGALAFVQEQFD
CCCEEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIFILIAAVPNIFGFKWSD
CCCEEEEEECCCHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTNQINYMSTFLAAVVGLLIL
CCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNNVTIRLPEEVPPNIAQVFKDV
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
IPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFSATDGYIGLAIVFGAYAFFWFVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
IHGPSVVEPLIVAISYANIEANVQLVQAGMHADKILNPVTQTFVVTMGGTGATLVVPFMF
CCCCHHHHHHHHHHEECCCCCCHHHHHHCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHH
MWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPIVLNPIFFIPFVTAPIINVWIMKFFVD
HHHHHHHHCCEEECHHHCHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQMNSFSIILPWTTPAPIGIVMGTALAPLSFVLAITLIIIDTLIYYPFVKVYDHQILEE
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERKGNSSSELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLAN
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECCCCCHHHHHH
ALNKAAAEYNVPVKAAAGGYGAHREMLPEFNLVILAPQVASNFEDMKAETDKLGIKLAKT
HHHHHHHHCCCCEEECCCCCCHHHHHCCCCCEEEECCHHHCCHHHHHHHHHHHCEEEEEC
EGAQYIKLTRDGKGALAFVQEQFD
CCCEEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1400164; 8277252; 12397186 [H]