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The map label for this gene is pstI [H]

Identifier: 221231886

GI number: 221231886

Start: 1056435

End: 1058168

Strand: Reverse

Name: pstI [H]

Synonym: SPN23F_10780

Alternate gene names: 221231886

Gene position: 1058168-1056435 (Counterclockwise)

Preceding gene: 221231887

Following gene: 221231885

Centisome position: 47.64

GC content: 43.43

Gene sequence:

>1734_bases
ATGACAGAAATGCTTAAAGGAATCGCAGCATCTGACGGTGTTGCAGTTGCAAAAGCATATCTACTCGTTCAGCCGGATTT
GTCATTTGAGACTATTACAGTCGAAGATACAAACGCAGAAGAAGCTCGCCTTGATGCCGCTCTACAGGCATCACAAGACG
AGCTTTCTGTTATTCGCGAGAAAGCAGTAGGTACGCTCGGTGAAGAAGCAGCTCAAGTTTTTGATGCTCACTTAATGGTT
CTTGCTGACCCAGAAATGATCAGCCAAATCAAGGAAACTATCCGTGCGAAGAAAGTGAATGCAGAAGCAGGTCTGAAAGA
AGTTACAGATATGTTTATCACTATCTTTGAAGGCATGGAAGACAACCCATACATGCAAGAACGCGCAGCGGATATCCGCG
ACGTGACAAAACGTGTATTGGCAAACCTTCTTGGTAAAAAATTGCCAAACCCAGCTTCTATCAATGAAGAAGTGATTGTG
ATTGCGCATGACTTGACTCCTTCAGATACAGCTCAATTGGACAAAAACTTTGTAAAAGCTTTTGTAACCAACATTGGTGG
ACGTACAAGCCACTCAGCTATCATGGCACGTACACTTGAAATTGCTGCTGTATTAGGTACAAATAACATCACTGAAATCG
TTAAAGACGGTGACATCCTTGCTGTTAACTGGATCACTGGAGAAGTGATTATCAACCCAACAGATGAACAAGCGGCAGAA
TTTAAAGCAGCTGGTGAAGCCTATGCGAAACAAAAAGCTGAATGGGCACTTTTGAAAGATGCTCAAACAGTGACTGCTGA
CGGTAAACACTTCGAGTTGGCTGCCAATATTGGTACTCCAAAAGACGTTGAAGGTGTTAACAACAACGGTGCAGAAGCTG
TTGGACTTTACCGTACAGAGTTCTTGTACATGGATTCTCAAGACTTCCCAACTGAAGATGAGCAGTATGAAGCATACAAG
GCTGTTCTTGAAGGAATGAACGGTAAACCTGTTGTCGTTCGTACAATGGATATCGGTGGAGATAAGGAACTTCCTTACTT
CGATATGCCTCACGAAATGAACCCATTCCTTGGATTCCGTGCTCTTCGTATCTCTATCTCTGAGACTGGAGATGCTATGT
TCCGCACACAAATCCGTGCTCTTCTTCGTGCGTCTGTTCACGGTCAATTGCGTATCATGTTCCCAATGGTTGCGCTCTTG
AAAGAATTCCGTGCAGCGAAAGCAGTCTTTGATGAAGAAAAAGCAAACCTTCTTGCTGAAGGTGTTGCAGTTGCGGATAA
CATCCAAGTTGGTATCATGATTGAGATTCCTGCAGCGGCTATGCTTGCAGACCAATTTGCTAAAGAAGTTGACTTCTTCT
CAATTGGTACAAACGACTTGATCCAATATACAATGGCAGCAGACCGTATGAACGAACAAGTTTCATACCTTTACCAACCA
TACAACCCATCAATCCTACGCTTGATTAACAATGTGATCAAAGCAGCTCACGCTGAAGGTAAATGGGCTGGTATGTGTGG
TGAGATGGCTGGTGACCAACAAGCTGTTCCACTTCTTGTCGGAATGGGCTTGGATGAGTTCTCTATGTCAGCAACATCTG
TACTTCGTACACGCAGCTTGATGAAGAAACTCGACACAGCTAAGATGGAAGAGTACGCAAACCGTGCCCTTACAGAATGC
TCAACAATGGAAGAAGTTCTTGAACTTCAAAAAGAATACGTTAATTTTGATTAA

Upstream 100 bases:

>100_bases
AAGGTGCTGACGTAACTATCTCAGCTGAAGGTGCAGATGCAGATGACGCTATCGCTGCAATCTCAGAAACAATGGAAAAA
GAAGGATTGGCATAAGGGAA

Downstream 100 bases:

>100_bases
TCGACAAGTCCCTGCAACTCAGTTACAGGGATTTTTTTGATATTTTAAAAAGAATTTTCAAGAAAATCTTTTTTATAGAA
AGTCCAACCTTGAAAAAGTA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMV
LADPEMISQIKETIRAKKVNAEAGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGDILAVNWITGEVIINPTDEQAAE
FKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYK
AVLEGMNGKPVVVRTMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALL
KEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTEC
STMEEVLELQKEYVNFD

Sequences:

>Translated_577_residues
MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMV
LADPEMISQIKETIRAKKVNAEAGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGDILAVNWITGEVIINPTDEQAAE
FKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYK
AVLEGMNGKPVVVRTMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALL
KEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTEC
STMEEVLELQKEYVNFD
>Mature_576_residues
TEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVL
ADPEMISQIKETIRAKKVNAEAGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIVI
AHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGDILAVNWITGEVIINPTDEQAAEF
KAAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKA
VLEGMNGKPVVVRTMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALLK
EFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPY
NPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECS
TMEEVLELQKEYVNFD

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=573, Percent_Identity=47.4694589877836, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI48994992, Length=513, Percent_Identity=37.4269005847953, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1788726, Length=567, Percent_Identity=35.4497354497354, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1789193, Length=562, Percent_Identity=32.2064056939502, Blast_Score=268, Evalue=8e-73,
Organism=Escherichia coli, GI1787994, Length=420, Percent_Identity=29.0476190476191, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI226510935, Length=174, Percent_Identity=29.8850574712644, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63426; Mature: 63295

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDAALQASQDELSVIRE
CCHHHHHHCCCCCHHEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHH
KAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAEAGLKEVTDMFITIFEGME
HHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCC
DNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIVIAHDLTPSDTAQLDKNFVKA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
FVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGDILAVNWITGEVIINPTDEQAAE
HHHHCCCCCCHHHHHHHHHHHHHEECCCCHHHHHCCCCEEEEEEEEEEEEECCCCHHHHH
FKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNNNGAEAVGLYRTE
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
FLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDMPHEMNPFLGFR
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEE
ALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALLKEFRAAKAVFDEEKANLLAE
EEEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVAVADNIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
CCEEECCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSL
CCCHHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
MKKLDTAKMEEYANRALTECSTMEEVLELQKEYVNFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDAALQASQDELSVIRE
CHHHHHHCCCCCHHEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHH
KAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAEAGLKEVTDMFITIFEGME
HHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHCCCC
DNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIVIAHDLTPSDTAQLDKNFVKA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
FVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGDILAVNWITGEVIINPTDEQAAE
HHHHCCCCCCHHHHHHHHHHHHHEECCCCHHHHHCCCCEEEEEEEEEEEEECCCCHHHHH
FKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNNNGAEAVGLYRTE
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
FLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDMPHEMNPFLGFR
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCEE
ALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALLKEFRAAKAVFDEEKANLLAE
EEEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVAVADNIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
CCEEECCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSL
CCCHHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
MKKLDTAKMEEYANRALTECSTMEEVLELQKEYVNFD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA