Click here to switch to the map view.

The map label for this gene is hsdS [H]

Identifier: 221231344

GI number: 221231344

Start: 447892

End: 449442

Strand: Reverse

Name: hsdS [H]

Synonym: SPN23F_04610

Alternate gene names: 221231344

Gene position: 449442-447892 (Counterclockwise)

Preceding gene: 221231345

Following gene: 221231343

Centisome position: 20.23

GC content: 32.95

Gene sequence:

>1551_bases
ATGACACCAGAACAACTTAAAGCAAGTATTCTCCAAAGAGCGATGGAAGGGAAATTAGTGCCGCAAAATCCCAATGACGA
ACCTGCAAGTGAATTATTAAAGAGAATTAAAGCTGAAAAAGAAAAACTTATCAGTGAAGGAAAAATCAAACGAGATAAAA
AGGAAACTGAGATATTTCGTGGTGATGATGGGAAACATTATGGGAAGTTTGCTGATGGAAGTACTCAAGAAATTGATGTT
CCTTATGATATTCCTGATACTTGGGAGTGGGTGAGGATAAAATCAATTTATTGGAATTTTGGGCAAAATAAGCCAGAGAA
ATCCTTTAGGTATATAGATACGTCTAGTATTGATAGAAAAAAGAACATAATCAACTACAAAAATCTACAATATCTTTCAC
CTGAACAAGCGCCTTCCCGTGCTAGAAAATTAGTTTCGCAGAATAGTGTCTTATTTTCAACAGTTAGACCATATCTAAAA
AATATTGCTGTAGTTAGAGAACTTAAAGAGTATTTGATAGCTAGTACAGCATTTATTGTTTTGGATACTTTACTTAACGA
AACATATTTGAAATATTATTTATTATCAGATAATTTTATCAATCGTGTTAATAATAAATCTACTGGAACAAGTTATCCTG
CAATCAATGATTATAATTTTAATCTATTATTAATTGCTCTCCCCCCACTATCCGAACAACAACGAATAGTAGAAGCAATC
GAATCAGCTTTAGAAAAAGTAGATGAATATGCTGAAAGTTATAATAGACTAGAACAGCTAGATAAAGAATTTCCAGATAA
ACTAAAAAAATCTATTCTTCAATATGCTATGCAAGGAAAATTAGTTGAACAAGACCCAAATGATGAATCAGTCGAAGTTT
TACTTGAAAAAATACGAGCAGAAAAACAAAAACTCTTTGAAGAAGGCAAGATTAAAAAGAAAGATTTGGACATTTCTATT
GTTTCCCAAGGAGATGATAACTCTTATTATGAGGAAGTACCTTGTGAAATACCAGAAAGTTGGGAATGGGTGAGGTTAAA
CGATATAACCTCCTACATTCAAAGAGGGAAGTCTCCAAAATATTCAAATATTCCTATATACCCTGTGATTGCTCAGAAAT
GTAATCAATGGTCTGGCTTTTCTATTGACTTAGCAAGATTTATTGATCCCGAAACAGTTCATAGTTACCAGAAAGAAAGA
TTACTAAGAGATGGTGATTTAATGTGGAATTCAACAGGTCTAGGTACGTTAGGACGATTAGCTATTTACCATGAAAATAA
AAATCCTTATGGCTGGGCTGTTGCAGATAGCCATGTTACAGTTATAAGAGTCTTATCGGGAGTTATTAATTGCCATTTCA
TCTATAATTTTCTTTCCTCTCCTATTGTTCAGTCAGTTATTGAAGAAAAAGCGTCGGGTAGTACAAAACAAAAAGAATTA
TTAACGAAAACTATAAAAGAATATTTAATCCCCCTCCCACCCCTTCCTGAACAATCCAGAATCGTTGATAAAATCGAACA
ATTTTTCGCCCATATTGACGCACTAATTTAG

Upstream 100 bases:

>100_bases
CCTTTGAGTTGATTCAGAATTATCAAGCGGAAAGAGCAACTTTAAATCATAAGATTGATAATGTATTAGCTGATATTTTG
CAGTTGTTGGAGGACAAATA

Downstream 100 bases:

>100_bases
TATAATAGTTTTACGTGCGGTGGAATTTCTATCAAACATAAGATAGGAGTTTTACGTTGAAAGATTTTGAATTACATTTG
AAGAAAGCTGGTTTAGCTGA

Product: type I restriction-modification system S protein

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDV
PYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAI
ESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISI
VSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKER
LLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKEL
LTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI

Sequences:

>Translated_516_residues
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDV
PYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAI
ESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISI
VSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKER
LLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKEL
LTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI
>Mature_515_residues
TPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVP
YDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKN
IAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIE
SALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIV
SQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERL
LRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELL
TKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 59582; Mature: 59451

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFR
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHC
GDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRK
CCCCCCCCCCCCCCCCEECCCCCCCCCCHHEEHHHHHEECCCCCCCCCEEECCCCCCHHH
KNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIV
HHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAI
HHHHHHHHHHHEEEECCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
ESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPK
HHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCC
YSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRL
CCCCCCHHHHHHHCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHEE
AIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKEL
EEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFR
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHC
GDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRK
CCCCCCCCCCCCCCCCEECCCCCCCCCCHHEEHHHHHEECCCCCCCCCEEECCCCCCHHH
KNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIV
HHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAI
HHHHHHHHHHHEEEECCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
ESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPK
HHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCC
YSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRL
CCCCCCHHHHHHHCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHCCCEEECCCCCCCHHEE
AIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKEL
EEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]