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The map label for this gene is ponA [H]

Identifier: 221230929

GI number: 221230929

Start: 3236528

End: 3238654

Strand: Reverse

Name: ponA [H]

Synonym: MLBr_02688

Alternate gene names: 221230929

Gene position: 3238654-3236528 (Counterclockwise)

Preceding gene: 221230930

Following gene: 221230928

Centisome position: 99.1

GC content: 56.32

Gene sequence:

>2127_bases
GTGCCTGGATCGCGGTTAAATCGGACATGGCTCCTACACCTCAACCGGCAGGTGAACTGGCGCTGGGTGCGGTATTCATT
GTATCTCGCTGTGGTGGTGTTGTTGTTGTTACCGATCGTCACTTTCACCATGACCTACTTCATGGTGAAAGTTCCCAGGC
CAGGTGACATTCGTACTAACCAGGTCTCGACGATCCTGGCCAACAATGGCTTGGAGATCGCCAAAATTGTTCCACCCGAA
GGTAACCGGGTCGATGTCAACCTCAGCCAAGTACCGGTGCATGTACGCCAGGCGGTTATTGCTGCAGAGGATCGCAACTT
CTATTCAAACCCGGGGTTTGACTTCAGAGCCTCTGTGCGAGCGGTGCAAAACAACCTCTTCGGTAGCGGCGATCTGCAGG
GCGGGTCAACTATCACCCAGCAGTATGTGAAGAATGCGTTAGTCGGTTCTGCACAACACGGGTTTGACGGTTTAATGCGC
AAGACAAAGGAATTGGTCATCGCCATCAAAATGTCGGACGCATGGTCTAAAGACGATGTGCTACAATCTTATTTGAATAT
AATATACTTCGGTAGGGGAGCCTACGGAATTTCAGCTGCGGCCAAGGCCTATTTTGACAAACCCGTGGAGCAGCTCACCT
GCTCCGAGGGGGCACTGTTGGCGGCGCTAATTCGGCGGCCGTCGGTCCTAGACCCGGCTATCAATCTCAAGGGTGTTACG
GCCCGTTGGAACTGGGTGCTGGACGGCATGGTGGACATCAATGCTCTGTCGCCGAATGACCGCTCGGTACAGCTGTTTCC
CGCTACCGTGCCGCCTGACCAGGCTCGTGCGCAGAATCAGACCACCGGACCCAATGGACTCATCGAGCGTCAGGTGGTCA
AAGAGCTGTTCGAACTATTCAACATCGATCGGCAAACCTTGAACACCCAGGGGCTGCAAGTCATTACCACCATCGATCCG
CAGGCTCAACGGATAGTTGAGAAGGCGGTATCGAAATACCTTGACGGGCAGGATCCCGACATGCGTGCTGCCATCGTTTC
GATCGATCCACATAACGGTGCGATACGCGCCTACTATGGCGGTGCTGACGCCAACGGTTTTGACTTTGCCCAGGCTGGGC
TGCAAACCGGCTCTTCTTTCAAGGTATTTGCGCTCATCGCCGCGCTCGAGCAAGGTATCGGTCTGGGCTATCGGGTCGAC
AGCTCCCCGCTTACTGTCGATGGTATTAAGATCACCAACAATGGAGACGAGAGTTGTGGAAATTGCAATATCGCTGAGGC
GCTCAAGCTCTCGTTGAACACCTCCTATTACCGTCTGATGCTCAAACTTAAGAATGGACCGCAGGCTGTTGCGGACGCCG
CGCACCAAGCTGGTATCGTGAGCAGCTTCCCTGGTGTAGCGCACACCCTGTCAGAGGATGGTAAAGGAGGACCGCCCAAC
AACGGAATCGTTCTGGGCCAGTACGAAACCCGCGTGATCGATATGGCATCGGCATACGCCACGTTGGCTGCGTCCGGCGT
CTACCACCGACCGCACTTGGTGCAAAAAGTCGTTAATGCTGAGGGCCGGGTACTTTTCGACGCCAGCACTGCGGACAACA
GCGGCGACCAACGCATCGCCAAAGCTGTCGCCGATAACGTTACTGCGGCGATGCAGCCGATTGCCGGTTATTCGCGTGGC
CATAACTTAGCTGGCGGGCGGCCGTCGGCAGCCAAAACAGGCACGGTGCAGCTGGGCGATACCACCGCTAACAAGGATGC
TTGGATGGTCGGTTACACACCTTCGCTGTCGACGGCGGTGTGGGTTGGTACCGTCAAAGACAATGTGCCGCTGATAACCG
CTTCAGGAGAAGCGATTTACGGCTCCGGCCTGCCGTCGGACATCTGGAAGTCGACGATGGATGGCGCTCTGGAGGGTACC
CCTAACGAGGGCTTCCCCAAGCCGACTGAGGTAGGTGGCTACGCTGGTGTGCCGACACTGGCACCCCCGCCTCCTTTGAA
GAAGGTTATTCAGCCTACTATTGAAGTAGCGCCGGGCATAATCATACCGGTCGGCCCGCCCACGACGGTCACTGTCCCGC
CGACGCAGGCATTGCCCGACCTAACAACTTCAACAACGCCACCGTGA

Upstream 100 bases:

>100_bases
CCGGTCGGCAGCGACCGTGGGCGGTGGCAGGCGACGCCGTTTGCCGAGCGATCCACCGTCACCCAAGCAGCCCGGCCGCG
CTACTGGGCCTGGTTGGAGG

Downstream 100 bases:

>100_bases
TCAACGCTCAGACGCACAGTACCACTATCTCGCCGCGGCCGTTGGCCGCTGATCGCCAGAGCGCCGATAACCGTGATTGC
CCCAGCCGCACAGACTATTT

Product: penicillin-bonding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 708; Mature: 707

Protein sequence:

>708_residues
MPGSRLNRTWLLHLNRQVNWRWVRYSLYLAVVVLLLLPIVTFTMTYFMVKVPRPGDIRTNQVSTILANNGLEIAKIVPPE
GNRVDVNLSQVPVHVRQAVIAAEDRNFYSNPGFDFRASVRAVQNNLFGSGDLQGGSTITQQYVKNALVGSAQHGFDGLMR
KTKELVIAIKMSDAWSKDDVLQSYLNIIYFGRGAYGISAAAKAYFDKPVEQLTCSEGALLAALIRRPSVLDPAINLKGVT
ARWNWVLDGMVDINALSPNDRSVQLFPATVPPDQARAQNQTTGPNGLIERQVVKELFELFNIDRQTLNTQGLQVITTIDP
QAQRIVEKAVSKYLDGQDPDMRAAIVSIDPHNGAIRAYYGGADANGFDFAQAGLQTGSSFKVFALIAALEQGIGLGYRVD
SSPLTVDGIKITNNGDESCGNCNIAEALKLSLNTSYYRLMLKLKNGPQAVADAAHQAGIVSSFPGVAHTLSEDGKGGPPN
NGIVLGQYETRVIDMASAYATLAASGVYHRPHLVQKVVNAEGRVLFDASTADNSGDQRIAKAVADNVTAAMQPIAGYSRG
HNLAGGRPSAAKTGTVQLGDTTANKDAWMVGYTPSLSTAVWVGTVKDNVPLITASGEAIYGSGLPSDIWKSTMDGALEGT
PNEGFPKPTEVGGYAGVPTLAPPPPLKKVIQPTIEVAPGIIIPVGPPTTVTVPPTQALPDLTTSTTPP

Sequences:

>Translated_708_residues
MPGSRLNRTWLLHLNRQVNWRWVRYSLYLAVVVLLLLPIVTFTMTYFMVKVPRPGDIRTNQVSTILANNGLEIAKIVPPE
GNRVDVNLSQVPVHVRQAVIAAEDRNFYSNPGFDFRASVRAVQNNLFGSGDLQGGSTITQQYVKNALVGSAQHGFDGLMR
KTKELVIAIKMSDAWSKDDVLQSYLNIIYFGRGAYGISAAAKAYFDKPVEQLTCSEGALLAALIRRPSVLDPAINLKGVT
ARWNWVLDGMVDINALSPNDRSVQLFPATVPPDQARAQNQTTGPNGLIERQVVKELFELFNIDRQTLNTQGLQVITTIDP
QAQRIVEKAVSKYLDGQDPDMRAAIVSIDPHNGAIRAYYGGADANGFDFAQAGLQTGSSFKVFALIAALEQGIGLGYRVD
SSPLTVDGIKITNNGDESCGNCNIAEALKLSLNTSYYRLMLKLKNGPQAVADAAHQAGIVSSFPGVAHTLSEDGKGGPPN
NGIVLGQYETRVIDMASAYATLAASGVYHRPHLVQKVVNAEGRVLFDASTADNSGDQRIAKAVADNVTAAMQPIAGYSRG
HNLAGGRPSAAKTGTVQLGDTTANKDAWMVGYTPSLSTAVWVGTVKDNVPLITASGEAIYGSGLPSDIWKSTMDGALEGT
PNEGFPKPTEVGGYAGVPTLAPPPPLKKVIQPTIEVAPGIIIPVGPPTTVTVPPTQALPDLTTSTTPP
>Mature_707_residues
PGSRLNRTWLLHLNRQVNWRWVRYSLYLAVVVLLLLPIVTFTMTYFMVKVPRPGDIRTNQVSTILANNGLEIAKIVPPEG
NRVDVNLSQVPVHVRQAVIAAEDRNFYSNPGFDFRASVRAVQNNLFGSGDLQGGSTITQQYVKNALVGSAQHGFDGLMRK
TKELVIAIKMSDAWSKDDVLQSYLNIIYFGRGAYGISAAAKAYFDKPVEQLTCSEGALLAALIRRPSVLDPAINLKGVTA
RWNWVLDGMVDINALSPNDRSVQLFPATVPPDQARAQNQTTGPNGLIERQVVKELFELFNIDRQTLNTQGLQVITTIDPQ
AQRIVEKAVSKYLDGQDPDMRAAIVSIDPHNGAIRAYYGGADANGFDFAQAGLQTGSSFKVFALIAALEQGIGLGYRVDS
SPLTVDGIKITNNGDESCGNCNIAEALKLSLNTSYYRLMLKLKNGPQAVADAAHQAGIVSSFPGVAHTLSEDGKGGPPNN
GIVLGQYETRVIDMASAYATLAASGVYHRPHLVQKVVNAEGRVLFDASTADNSGDQRIAKAVADNVTAAMQPIAGYSRGH
NLAGGRPSAAKTGTVQLGDTTANKDAWMVGYTPSLSTAVWVGTVKDNVPLITASGEAIYGSGLPSDIWKSTMDGALEGTP
NEGFPKPTEVGGYAGVPTLAPPPPLKKVIQPTIEVAPGIIIPVGPPTTVTVPPTQALPDLTTSTTPP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=331, Percent_Identity=32.3262839879154, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI1786343, Length=570, Percent_Identity=27.719298245614, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788867, Length=575, Percent_Identity=25.0434782608696, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=36.4705882352941, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 75663; Mature: 75532

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGSRLNRTWLLHLNRQVNWRWVRYSLYLAVVVLLLLPIVTFTMTYFMVKVPRPGDIRTN
CCCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
QVSTILANNGLEIAKIVPPEGNRVDVNLSQVPVHVRQAVIAAEDRNFYSNPGFDFRASVR
CEEEEEECCCCEEEEEECCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHH
AVQNNLFGSGDLQGGSTITQQYVKNALVGSAQHGFDGLMRKTKELVIAIKMSDAWSKDDV
HHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEEEEEECCCCCHHHH
LQSYLNIIYFGRGAYGISAAAKAYFDKPVEQLTCSEGALLAALIRRPSVLDPAINLKGVT
HHHHHHEEEECCCCCCHHHHHHHHHHCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEE
ARWNWVLDGMVDINALSPNDRSVQLFPATVPPDQARAQNQTTGPNGLIERQVVKELFELF
EEEEEEEECEEEEEEECCCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
NIDRQTLNTQGLQVITTIDPQAQRIVEKAVSKYLDGQDPDMRAAIVSIDPHNGAIRAYYG
CCCHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEC
GADANGFDFAQAGLQTGSSFKVFALIAALEQGIGLGYRVDSSPLTVDGIKITNNGDESCG
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEECCCCCCCC
NCNIAEALKLSLNTSYYRLMLKLKNGPQAVADAAHQAGIVSSFPGVAHTLSEDGKGGPPN
CCCHHHHHEECCCCCEEEEEEEECCCCHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCC
NGIVLGQYETRVIDMASAYATLAASGVYHRPHLVQKVVNAEGRVLFDASTADNSGDQRIA
CCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
KAVADNVTAAMQPIAGYSRGHNLAGGRPSAAKTGTVQLGDTTANKDAWMVGYTPSLSTAV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCEE
WVGTVKDNVPLITASGEAIYGSGLPSDIWKSTMDGALEGTPNEGFPKPTEVGGYAGVPTL
EEEEECCCCEEEEECCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
APPPPLKKVIQPTIEVAPGIIIPVGPPTTVTVPPTQALPDLTTSTTPP
CCCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PGSRLNRTWLLHLNRQVNWRWVRYSLYLAVVVLLLLPIVTFTMTYFMVKVPRPGDIRTN
CCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
QVSTILANNGLEIAKIVPPEGNRVDVNLSQVPVHVRQAVIAAEDRNFYSNPGFDFRASVR
CEEEEEECCCCEEEEEECCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHH
AVQNNLFGSGDLQGGSTITQQYVKNALVGSAQHGFDGLMRKTKELVIAIKMSDAWSKDDV
HHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEEEEEECCCCCHHHH
LQSYLNIIYFGRGAYGISAAAKAYFDKPVEQLTCSEGALLAALIRRPSVLDPAINLKGVT
HHHHHHEEEECCCCCCHHHHHHHHHHCHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEE
ARWNWVLDGMVDINALSPNDRSVQLFPATVPPDQARAQNQTTGPNGLIERQVVKELFELF
EEEEEEEECEEEEEEECCCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
NIDRQTLNTQGLQVITTIDPQAQRIVEKAVSKYLDGQDPDMRAAIVSIDPHNGAIRAYYG
CCCHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEC
GADANGFDFAQAGLQTGSSFKVFALIAALEQGIGLGYRVDSSPLTVDGIKITNNGDESCG
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEECCCCCCCC
NCNIAEALKLSLNTSYYRLMLKLKNGPQAVADAAHQAGIVSSFPGVAHTLSEDGKGGPPN
CCCHHHHHEECCCCCEEEEEEEECCCCHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCC
NGIVLGQYETRVIDMASAYATLAASGVYHRPHLVQKVVNAEGRVLFDASTADNSGDQRIA
CCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
KAVADNVTAAMQPIAGYSRGHNLAGGRPSAAKTGTVQLGDTTANKDAWMVGYTPSLSTAV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCEE
WVGTVKDNVPLITASGEAIYGSGLPSDIWKSTMDGALEGTPNEGFPKPTEVGGYAGVPTL
EEEEECCCCEEEEECCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
APPPPLKKVIQPTIEVAPGIIIPVGPPTTVTVPPTQALPDLTTSTTPP
CCCCCHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]