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The map label for this gene is pckA

Identifier: 221230895

GI number: 221230895

Start: 3135143

End: 3136972

Strand: Direct

Name: pckA

Synonym: MLBr_02624

Alternate gene names: 221230895

Gene position: 3135143-3136972 (Clockwise)

Preceding gene: 221230888

Following gene: 221230896

Centisome position: 95.93

GC content: 61.15

Gene sequence:

>1830_bases
ATGACTTCAGCGACTATTCCCGGTCTGGATACCGCTCCAACAAACCACCAGAAGTTGCTGTCGTGGCTAGAGGAGGTCGC
TGAACTGACACATCCTGCCCAGGTGGTTTTCGCTGACGGTTCCGACGAAGAATGGCAACAGCTCACCGAGCGACTGGTAG
CGGCGGGCACCTTCAAGAAGCTGAACGATGAAAAACACCCCAACTCCTACCTGGCGCTGTCCGATCCCTCTGACGTGGCG
CGGGTGGAGTCACGGACCTTTATCTGTTCCGAGCGTGAGATCGATGCCGGACCCACCAACAACTGGATGGACCCCGCTGA
AATGCGGTCTACCATGACCGAGCTGTACCGCGGTTGCATGCGCGGCCGCACCATGTGGGTGGTGCCCTTCTGCATGGGTC
CGCCGGGTGCTGAGGACCCGAAGTTGGGCGTGGAGATCACCGACTCCGAATACGTTGTTGCGTCGATGAAGGTTATGACT
CGGATGGGCACATCCGCCCTAGAGAAGATCGGCGACGACGGGTTCTTCGTCAAGGCCCTGCATTCGGTGGGCGCCCCGCT
ACAGCCGGGTCAACAAGACATGCCGTGGCCGTGCAACGAAACCAAATACATCACCCATTTCCCGGAGACCCGCGAGATCT
GGAGCTACGGCTCCGGCTACGGCGGCAACGCACTGTTAGGGAAGAAGTGCTACTCGCTGCGAATCGCCTCGGCGATGGCC
CACGACGAGGGCTGGCTTGCCGAGCACATGCTGATCCTTAAGCTCATTTCCCCGGAGAACAAGGCGTACTACTTCGCGGC
GGCGTTCCCGTCGGCCTGCGGCAAGACCAACCTCGCCATGCTGCAACCGACAATCCCGGGCTGGCGCGCTGAAACTCTCG
GGGACGACATCGCCTGGATGCGATTCGGCAAGGACGGCCGACTGTACGCGGTCAACCCCGAGTTCGGATTCTTCGGCGTC
GCACCGGGCACCAACTGGAAGTCCAACCCGAACGCCATGCGCACCATCGCCGCGGGTAACACCGTCTTCACCAATGTCGC
GCTCACCGACGACAACGACGTATGGTGGGAGGGCCTGGAAGGAGAGCCTGCGCATCTGATCGACTGGAAGGGCAACGACT
GGTACGCTGGGAAGACGGAGACCCCCGCGGCACATTCGAATTCGCGCTACTGCACGCCGATGTCGCAGTGCCCGATCTTG
GCGCCGGAATGGGACGATCCGCGGGGTGTGCCGATCTCAGTCATTCTGTTCGGCGCCCGCCGCAAGACTACAGTGCCGTT
GGTAACGCAAGCTCGCAACTGGCAGCACGGCGTTTTCATGGGAGCCACCATGGGTAGCGAACAGACCGCCGCAGCCGAAG
GCAAGGTCGGTACGGTTCGGCGCGACCCGATGGCCATGTTGCCATTCATGGGCTACCACGTCGGCGACTACTTTCAGCAC
TGGATCGATCTGGGCAAACACTCCGACGAATCCAATCTACCGAAGGTGTTCTTCGTCAACTGGTTCCGCCGCGGCGAAGG
CGGCAAATTCTTATGGCCGGGCTTCGGCGAGAACAGCCGGGTGTTGAAGTGGATCGTTGACCGCATCGAGCACAAGGCAG
GCGGACAGGACACCCTGATCGGCATCGTGCCGACCGCCACGGACCTGGACCTGGACGGACTCGACATCAGCAGCGACGAC
GTTGCCAAGGCTCTCGCGGTCGACCCCGCCGAGTGGCGCAATGAACTGCCGTTGATTGAAGAGTGGTTCGAGTTCGTCGG
CGACAAGTTGCCCTCTGGCATGCAGGATGAATTCGAAGCACTCAAGCAGCGGCTGGCAAAGGAGGACTAA

Upstream 100 bases:

>100_bases
CTGTACATAACGCGTTTGTGTTGGCAAATACAGGCGAGTGCATCAACAACCACGAGAAGCCGACGAAATACGTAAATGAA
TTCAGGAGAGGGAGACTTCG

Downstream 100 bases:

>100_bases
CAGCCAATCGTGTATCGTCGCAACGACTTCGATAGGTATACTACACCCGATCCGGGGCCGGCTACCGAGTTGTCCAGATT
CGAGAAAGCGCATCACTTCA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MTSATIPGLDTAPTNHQKLLSWLEEVAELTHPAQVVFADGSDEEWQQLTERLVAAGTFKKLNDEKHPNSYLALSDPSDVA
RVESRTFICSEREIDAGPTNNWMDPAEMRSTMTELYRGCMRGRTMWVVPFCMGPPGAEDPKLGVEITDSEYVVASMKVMT
RMGTSALEKIGDDGFFVKALHSVGAPLQPGQQDMPWPCNETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGV
APGTNWKSNPNAMRTIAAGNTVFTNVALTDDNDVWWEGLEGEPAHLIDWKGNDWYAGKTETPAAHSNSRYCTPMSQCPIL
APEWDDPRGVPISVILFGARRKTTVPLVTQARNWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFMGYHVGDYFQH
WIDLGKHSDESNLPKVFFVNWFRRGEGGKFLWPGFGENSRVLKWIVDRIEHKAGGQDTLIGIVPTATDLDLDGLDISSDD
VAKALAVDPAEWRNELPLIEEWFEFVGDKLPSGMQDEFEALKQRLAKED

Sequences:

>Translated_609_residues
MTSATIPGLDTAPTNHQKLLSWLEEVAELTHPAQVVFADGSDEEWQQLTERLVAAGTFKKLNDEKHPNSYLALSDPSDVA
RVESRTFICSEREIDAGPTNNWMDPAEMRSTMTELYRGCMRGRTMWVVPFCMGPPGAEDPKLGVEITDSEYVVASMKVMT
RMGTSALEKIGDDGFFVKALHSVGAPLQPGQQDMPWPCNETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGV
APGTNWKSNPNAMRTIAAGNTVFTNVALTDDNDVWWEGLEGEPAHLIDWKGNDWYAGKTETPAAHSNSRYCTPMSQCPIL
APEWDDPRGVPISVILFGARRKTTVPLVTQARNWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFMGYHVGDYFQH
WIDLGKHSDESNLPKVFFVNWFRRGEGGKFLWPGFGENSRVLKWIVDRIEHKAGGQDTLIGIVPTATDLDLDGLDISSDD
VAKALAVDPAEWRNELPLIEEWFEFVGDKLPSGMQDEFEALKQRLAKED
>Mature_608_residues
TSATIPGLDTAPTNHQKLLSWLEEVAELTHPAQVVFADGSDEEWQQLTERLVAAGTFKKLNDEKHPNSYLALSDPSDVAR
VESRTFICSEREIDAGPTNNWMDPAEMRSTMTELYRGCMRGRTMWVVPFCMGPPGAEDPKLGVEITDSEYVVASMKVMTR
MGTSALEKIGDDGFFVKALHSVGAPLQPGQQDMPWPCNETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMAH
DEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGVA
PGTNWKSNPNAMRTIAAGNTVFTNVALTDDNDVWWEGLEGEPAHLIDWKGNDWYAGKTETPAAHSNSRYCTPMSQCPILA
PEWDDPRGVPISVILFGARRKTTVPLVTQARNWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFMGYHVGDYFQHW
IDLGKHSDESNLPKVFFVNWFRRGEGGKFLWPGFGENSRVLKWIVDRIEHKAGGQDTLIGIVPTATDLDLDGLDISSDDV
AKALAVDPAEWRNELPLIEEWFEFVGDKLPSGMQDEFEALKQRLAKED

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI187281517, Length=615, Percent_Identity=50.4065040650407, Blast_Score=595, Evalue=1e-170,
Organism=Homo sapiens, GI66346721, Length=602, Percent_Identity=50.6644518272425, Blast_Score=582, Evalue=1e-166,
Organism=Homo sapiens, GI66346723, Length=371, Percent_Identity=52.8301886792453, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71989645, Length=607, Percent_Identity=46.4579901153213, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI71989658, Length=607, Percent_Identity=46.4579901153213, Blast_Score=553, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI71989653, Length=594, Percent_Identity=46.6329966329966, Blast_Score=544, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17555492, Length=620, Percent_Identity=46.9354838709677, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI32564411, Length=547, Percent_Identity=49.5429616087751, Blast_Score=525, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI32564407, Length=528, Percent_Identity=49.6212121212121, Blast_Score=494, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71989649, Length=487, Percent_Identity=49.2813141683778, Blast_Score=481, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI32564409, Length=373, Percent_Identity=47.7211796246649, Blast_Score=332, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17540956, Length=329, Percent_Identity=23.7082066869301, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24655085, Length=600, Percent_Identity=49.8333333333333, Blast_Score=583, Evalue=1e-166,
Organism=Drosophila melanogaster, GI24655082, Length=601, Percent_Identity=50.2495840266223, Blast_Score=575, Evalue=1e-164,
Organism=Drosophila melanogaster, GI161077226, Length=558, Percent_Identity=51.9713261648746, Blast_Score=558, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24655088, Length=504, Percent_Identity=51.3888888888889, Blast_Score=516, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_MYCLB (B8ZTI5)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002504311.1
- ProteinModelPortal:   B8ZTI5
- SMR:   B8ZTI5
- EnsemblBacteria:   EBMYCT00000086296
- GeneID:   7325536
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015941
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 67732; Mature: 67601

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 273-273 BINDING 81-81 BINDING 222-222 BINDING 229-229 BINDING 271-271 BINDING 389-389 BINDING 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSATIPGLDTAPTNHQKLLSWLEEVAELTHPAQVVFADGSDEEWQQLTERLVAAGTFKK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
LNDEKHPNSYLALSDPSDVARVESRTFICSEREIDAGPTNNWMDPAEMRSTMTELYRGCM
CCCCCCCCCEEEECCCHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RGRTMWVVPFCMGPPGAEDPKLGVEITDSEYVVASMKVMTRMGTSALEKIGDDGFFVKAL
CCCEEEEEEEECCCCCCCCCCCCEEECCCCEEHHHHHHHHHHCHHHHHHHCCCCHHHHHH
HSVGAPLQPGQQDMPWPCNETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWM
CCCCHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCHHHCCCCEEEE
RFGKDGRLYAVNPEFGFFGVAPGTNWKSNPNAMRTIAAGNTVFTNVALTDDNDVWWEGLE
EECCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCEEECCC
GEPAHLIDWKGNDWYAGKTETPAAHSNSRYCTPMSQCPILAPEWDDPRGVPISVILFGAR
CCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHCCEECCCCCCCCCCEEEEEEECCC
RKTTVPLVTQARNWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFMGYHVGDYFQH
CCCCCCEEECCCCCCCCEEEEECCCCCCHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHH
WIDLGKHSDESNLPKVFFVNWFRRGEGGKFLWPGFGENSRVLKWIVDRIEHKAGGQDTLI
HHHHCCCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEE
GIVPTATDLDLDGLDISSDDVAKALAVDPAEWRNELPLIEEWFEFVGDKLPSGMQDEFEA
EEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHH
LKQRLAKED
HHHHHCCCC
>Mature Secondary Structure 
TSATIPGLDTAPTNHQKLLSWLEEVAELTHPAQVVFADGSDEEWQQLTERLVAAGTFKK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
LNDEKHPNSYLALSDPSDVARVESRTFICSEREIDAGPTNNWMDPAEMRSTMTELYRGCM
CCCCCCCCCEEEECCCHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RGRTMWVVPFCMGPPGAEDPKLGVEITDSEYVVASMKVMTRMGTSALEKIGDDGFFVKAL
CCCEEEEEEEECCCCCCCCCCCCEEECCCCEEHHHHHHHHHHCHHHHHHHCCCCHHHHHH
HSVGAPLQPGQQDMPWPCNETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWM
CCCCHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCHHHCCCCEEEE
RFGKDGRLYAVNPEFGFFGVAPGTNWKSNPNAMRTIAAGNTVFTNVALTDDNDVWWEGLE
EECCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCEEECCC
GEPAHLIDWKGNDWYAGKTETPAAHSNSRYCTPMSQCPILAPEWDDPRGVPISVILFGAR
CCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHCCEECCCCCCCCCCEEEEEEECCC
RKTTVPLVTQARNWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFMGYHVGDYFQH
CCCCCCEEECCCCCCCCEEEEECCCCCCHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHH
WIDLGKHSDESNLPKVFFVNWFRRGEGGKFLWPGFGENSRVLKWIVDRIEHKAGGQDTLI
HHHHCCCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEE
GIVPTATDLDLDGLDISSDDVAKALAVDPAEWRNELPLIEEWFEFVGDKLPSGMQDEFEA
EEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHH
LKQRLAKED
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA