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The map label for this gene is fadB [H]

Identifier: 221230829

GI number: 221230829

Start: 2974487

End: 2975146

Strand: Direct

Name: fadB [H]

Synonym: MLBr_02498

Alternate gene names: 221230829

Gene position: 2974487-2975146 (Clockwise)

Preceding gene: 221230828

Following gene: 221230830

Centisome position: 91.02

GC content: 59.39

Gene sequence:

>660_bases
ATGACCATCGCCGTGCATTATGACGAAAAAATCGCCATCCTCGAACTTGGTGATGACGAAAATCGTTTCACATCTGTCTT
TCTTGAAGAAATCAACTCCCACCTCGACGAAATCATAGCCAGCGGTGCCAACGGCTTGGTGACGACGGCACTTGGCAAGT
TCTATTCCAACGGGCTAGACCTGGACTGGCTGTCCGCACACCGTGACCAGACTCAGTGGTATGTCGGCAAGGTCCAGGGA
CTCCTGGCTCGCCTCCTCACGCTGCCCATCCCTACCGCAGCGGCCGTCGTTGGTCACGCGTTCGGCGCCGGAGCAATGTT
GGCCATGGCGCACGATTTTCGTGTTATGCGTGCAGATCGCGGCTACTTCTGTTTTCCCGAAGTGGATATCCGCATCCCGT
TCACGCCCGGCATGGCCGCACTGATACAAGCCAAACTTGCTCCCCAGGCAGCAACTGCGTCAATGACCACCGGGCGCCGG
TTCGGCGGCGGCGAAGCCGCCCGCCTCGGAATTGTCGACAATACGGCAGCCGAGGATGCCGTTACCGCCGTGGCGGTCGA
CCTGCTTCATCCGCTCGGCGGCAAAGATCCAGGCACGCTGGGCGTCATCAAACAGACCATGTTCGGCCACGCCGCGGCTG
CTCTTTCTGCCAATGCCTAA

Upstream 100 bases:

>100_bases
GCAACAAGACGGCACGAAAGCATCTCCATGCAGCAGTGCGCGCGGTTCTGGAGGTCGGACCACCACCGTAAAGTGACCTA
CAACAGAAAGGAAGAACCGC

Downstream 100 bases:

>100_bases
GAGCTGAAAGAGGATTAACATTACGCTGGTTCTTCCTGAGGCTGATCGATATTTCATCCGGTGGCGAGATCGCAACAGTC
GACCATCTCGACTTCGAGCC

Product: putative enoyl-CoA hydratase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTIAVHYDEKIAILELGDDENRFTSVFLEEINSHLDEIIASGANGLVTTALGKFYSNGLDLDWLSAHRDQTQWYVGKVQG
LLARLLTLPIPTAAAVVGHAFGAGAMLAMAHDFRVMRADRGYFCFPEVDIRIPFTPGMAALIQAKLAPQAATASMTTGRR
FGGGEAARLGIVDNTAAEDAVTAVAVDLLHPLGGKDPGTLGVIKQTMFGHAAAALSANA

Sequences:

>Translated_219_residues
MTIAVHYDEKIAILELGDDENRFTSVFLEEINSHLDEIIASGANGLVTTALGKFYSNGLDLDWLSAHRDQTQWYVGKVQG
LLARLLTLPIPTAAAVVGHAFGAGAMLAMAHDFRVMRADRGYFCFPEVDIRIPFTPGMAALIQAKLAPQAATASMTTGRR
FGGGEAARLGIVDNTAAEDAVTAVAVDLLHPLGGKDPGTLGVIKQTMFGHAAAALSANA
>Mature_218_residues
TIAVHYDEKIAILELGDDENRFTSVFLEEINSHLDEIIASGANGLVTTALGKFYSNGLDLDWLSAHRDQTQWYVGKVQGL
LARLLTLPIPTAAAVVGHAFGAGAMLAMAHDFRVMRADRGYFCFPEVDIRIPFTPGMAALIQAKLAPQAATASMTTGRRF
GGGEAARLGIVDNTAAEDAVTAVAVDLLHPLGGKDPGTLGVIKQTMFGHAAAALSANA

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the 3- hydroxyacyl-CoA dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI221142681, Length=161, Percent_Identity=31.6770186335404, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012799
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =5.3.3.8; =1.1.1.35 [H]

Molecular weight: Translated: 23120; Mature: 22989

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAVHYDEKIAILELGDDENRFTSVFLEEINSHLDEIIASGANGLVTTALGKFYSNGLD
CEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
LDWLSAHRDQTQWYVGKVQGLLARLLTLPIPTAAAVVGHAFGAGAMLAMAHDFRVMRADR
CHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHEEEECCC
GYFCFPEVDIRIPFTPGMAALIQAKLAPQAATASMTTGRRFGGGEAARLGIVDNTAAEDA
CEEECCCEEEEECCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCHHHH
VTAVAVDLLHPLGGKDPGTLGVIKQTMFGHAAAALSANA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIAVHYDEKIAILELGDDENRFTSVFLEEINSHLDEIIASGANGLVTTALGKFYSNGLD
EEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
LDWLSAHRDQTQWYVGKVQGLLARLLTLPIPTAAAVVGHAFGAGAMLAMAHDFRVMRADR
CHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHEEEECCC
GYFCFPEVDIRIPFTPGMAALIQAKLAPQAATASMTTGRRFGGGEAARLGIVDNTAAEDA
CEEECCCEEEEECCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCHHHH
VTAVAVDLLHPLGGKDPGTLGVIKQTMFGHAAAALSANA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA