BacMap

Definition	Mycobacterium leprae Br4923 chromosome, complete genome.
Accession	NC_011896
Length	3,268,071

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The map label for this gene is echA8

Identifier: 221230769

GI number: 221230769

Start: 2872726

End: 2873499

Strand: Direct

Name: echA8

Synonym: MLBr_02402

Alternate gene names: 221230769

Gene position: 2872726-2873499 (Clockwise)

Preceding gene: 221230768

Following gene: 221230770

Centisome position: 87.9

GC content: 57.24

Gene sequence:

>774_bases
ATGAAGTACGACACAATCCTCGTTGACGGCTACCAGCGAGTTGGCATCATCACACTGAACCGGCCACAGGCTCTGAACGC
ACTCAATAGCCAGATGATGAACGAAATCACTAATGCAGCAAAAGAATTAGACATCGATCCCGATGTTGGGGCGATCCTCA
TTACTGGTTCACCCAAGGTTTTTGCTGCCGGCGCCGACATCAAAGAGATGGCCAGTTTAACGTTCACCGACGCATTTGAC
GCTGATTTTTTCTCCGCATGGGGCAAGCTGGCCGCCGTGCGTACCCCAATGATCGCCGCGGTGGCCGGATATGCACTCGG
TGGTGGCTGCGAGTTAGCGATGATGTGCGATCTGCTTATCGCCGCCGACACAGCGAAATTCGGTCAGCCAGAAATCAAAC
TCGGCGTTCTGCCCGGTATGGGAGGCTCCCAGCGCCTAACCCGAGCGATCGGCAAGGCCAAAGCAATGGACCTGATCTTG
ACCGGACGCACCATCGACGCGGCCGAAGCCGAACGCAGCGGCCTGGTTTCGCGGGTGGTGCTGGCCGACGATTTACTGCC
CGAGGCCAAGGCTGTCGCTACTACCATTTCGCAGATGTCTCGGTCGGCTACCCGGATGGCCAAGGAAGCAGTCAATCGGT
CTTTCGAATCCACGCTGGCTGAAGGATTGCTCCACGAACGCCGGCTTTTCCATTCGACCTTCGTGACAGACGACCAGTCC
GAGGGCATGGCGGCATTCATCGAGAAACGCGCTCCCCAGTTTACCCACCGATGA

Upstream 100 bases:

>100_bases
GACGACACTGGCCGAAGTCACTGCAGCCGACATCTGTGGATATTTCGAGCCGGTTGACGATGACTTGAGTTTCTAGAAAG
GCAATTTGGTGACGAATAAC

Downstream 100 bases:

>100_bases
GCGAAGCTGGCTCTGCAACAGCCGCCGTTGGCGATGAAGCGGTTGACAGCGAATCAGCGATCGATACCGCGGAATCCGCC
GGTGCTAACGCAACACCCAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKYDTILVDGYQRVGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGSPKVFAAGADIKEMASLTFTDAFD
ADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLIL
TGRTIDAAEAERSGLVSRVVLADDLLPEAKAVATTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTFVTDDQS
EGMAAFIEKRAPQFTHR

Sequences:

>Translated_257_residues
MKYDTILVDGYQRVGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGSPKVFAAGADIKEMASLTFTDAFD
ADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLIL
TGRTIDAAEAERSGLVSRVVLADDLLPEAKAVATTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTFVTDDQS
EGMAAFIEKRAPQFTHR
>Mature_257_residues
MKYDTILVDGYQRVGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGSPKVFAAGADIKEMASLTFTDAFD
ADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLIAADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLIL
TGRTIDAAEAERSGLVSRVVLADDLLPEAKAVATTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTFVTDDQS
EGMAAFIEKRAPQFTHR

Specific function: Could possibly oxidize fatty acids using specific components

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=241, Percent_Identity=53.1120331950208, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=35.7976653696498, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI68989263, Length=225, Percent_Identity=32.8888888888889, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=28.3400809716599, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI20127408, Length=222, Percent_Identity=32.4324324324324, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=32.9268292682927, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI70995211, Length=264, Percent_Identity=30.6818181818182, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI213417737, Length=227, Percent_Identity=28.6343612334802, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI157694516, Length=227, Percent_Identity=28.6343612334802, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI37594471, Length=247, Percent_Identity=27.1255060728745, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI37594469, Length=261, Percent_Identity=26.4367816091954, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI261878539, Length=126, Percent_Identity=38.8888888888889, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI260274832, Length=260, Percent_Identity=24.2307692307692, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI45643119, Length=260, Percent_Identity=24.2307692307692, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI260275230, Length=260, Percent_Identity=24.2307692307692, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI25453481, Length=265, Percent_Identity=25.2830188679245, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI51538573, Length=265, Percent_Identity=25.2830188679245, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4757966, Length=192, Percent_Identity=30.2083333333333, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538581, Length=192, Percent_Identity=30.2083333333333, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757968, Length=265, Percent_Identity=24.9056603773585, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI49355787, Length=265, Percent_Identity=24.9056603773585, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI157694520, Length=209, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI62530384, Length=195, Percent_Identity=27.6923076923077, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787659, Length=252, Percent_Identity=42.4603174603175, Blast_Score=213, Evalue=9e-57,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=35.6, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=34.9593495934959, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790281, Length=197, Percent_Identity=34.010152284264, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787660, Length=248, Percent_Identity=33.4677419354839, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=34.010152284264, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI87082183, Length=268, Percent_Identity=30.2238805970149, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=52.8455284552846, Blast_Score=268, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=47.1311475409836, Blast_Score=240, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=33.1818181818182, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=241, Percent_Identity=29.8755186721992, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=248, Percent_Identity=30.6451612903226, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17508953, Length=222, Percent_Identity=31.5315315315315, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144276, Length=222, Percent_Identity=31.5315315315315, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17508951, Length=222, Percent_Identity=31.5315315315315, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=277, Percent_Identity=27.4368231046931, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144160, Length=283, Percent_Identity=28.6219081272085, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=283, Percent_Identity=28.6219081272085, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=205, Percent_Identity=31.7073170731707, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=256, Percent_Identity=28.515625, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=26.0536398467433, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=199, Percent_Identity=26.6331658291457, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=29.5081967213115, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=54.6938775510204, Blast_Score=280, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=54.6938775510204, Blast_Score=280, Evalue=8e-76,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=36.9047619047619, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=27.5862068965517, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=32.9317269076305, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357171, Length=258, Percent_Identity=28.6821705426357, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=31.924882629108, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=28.3422459893048, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI28571730, Length=227, Percent_Identity=28.1938325991189, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI28571729, Length=227, Percent_Identity=28.1938325991189, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921000, Length=215, Percent_Identity=34.8837209302326, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24583077, Length=215, Percent_Identity=34.8837209302326, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583079, Length=215, Percent_Identity=34.8837209302326, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583165, Length=177, Percent_Identity=26.5536723163842, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19921018, Length=183, Percent_Identity=26.775956284153, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ECHA8_MYCLE (O07137)

Other databases:

- EMBL:   Y13803
- EMBL:   AL583925
- PIR:   F87209
- RefSeq:   NP_302555.1
- ProteinModelPortal:   O07137
- SMR:   O07137
- EnsemblBacteria:   EBMYCT00000029295
- GeneID:   908604
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2402
- NMPDR:   fig|272631.1.peg.1427
- Leproma:   ML2402
- GeneTree:   EBGT00050000015506
- HOGENOM:   HBG748731
- OMA:   CELAMIC
- ProtClustDB:   PRK05862
- BioCyc:   MLEP272631:ML2402-MONOMER
- BRENDA:   4.2.1.17
- InterPro:   IPR001753
- InterPro:   IPR018376

Pfam domain/function: PF00378 ECH

EC number: =4.2.1.17

Molecular weight: Translated: 27517; Mature: 27517

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDTILVDGYQRVGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGSPKV
CCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCE
FAAGADIKEMASLTFTDAFDADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLI
EECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVV
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHH
LADDLLPEAKAVATTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTFVTDDQS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMAAFIEKRAPQFTHR
CCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKYDTILVDGYQRVGIITLNRPQALNALNSQMMNEITNAAKELDIDPDVGAILITGSPKV
CCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCE
FAAGADIKEMASLTFTDAFDADFFSAWGKLAAVRTPMIAAVAGYALGGGCELAMMCDLLI
EECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AADTAKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTIDAAEAERSGLVSRVV
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHH
LADDLLPEAKAVATTISQMSRSATRMAKEAVNRSFESTLAEGLLHERRLFHSTFVTDDQS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMAAFIEKRAPQFTHR
CCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002