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The map label for this gene is leuA

Identifier: 221230726

GI number: 221230726

Start: 2754565

End: 2756388

Strand: Direct

Name: leuA

Synonym: MLBr_02324

Alternate gene names: 221230726

Gene position: 2754565-2756388 (Clockwise)

Preceding gene: 221230720

Following gene: 221230727

Centisome position: 84.29

GC content: 60.53

Gene sequence:

>1824_bases
GTGGCCAGCTTTTCTGAGTCTTTGAGCCAGGATCCAGCAGATGCCTATAAATCGGCACCCTCCATCACCAAACCCATGGG
CCCGCCCAGTCCCGGCCAACCCCAGTGGAATCCCCAACGAGCTACATCGATGCCAGTCTTCCGGTACCGGCCGTTCGCCA
AGGAAGTCGAGCCTATCCGGCTAGTTGACCGCACCTGGCCCGACCGCGTCATTGATTGCGCGCCTCTGTGGTGTGCGGTG
GATCTGCGGGACGGCAACCAAGCGCTGATCGACCCGATGAGCCCAATCTGCAAACGCCGCATGTTCGACCTGCTGGTTCG
CATGGGCTACAAGGAGATCGAGGTCGGATTCCCGTCGGCCAGCCAGACTGACTTCGACTTCGTCCGCGAGATCATCACCG
ACGGCACTATTCCGGACGACGTCACCATCCAGGTTCTGACCCAATGCCGACCGGAACTGATTGAGCGCACCTTCGAAGCC
TGCGAAAACGCGTCGCGGGTTATAGTGCACTTCTACAACTCAACGTCAATTCTGCAGCGCCGCGTGGTTTTTCGCGCCGA
CCAAGCCACGGTAAAGGCTATCGCGACCGACGGTGCTCGTAAGTGTGTCGAAGAGGCGTTCAAGTACCCGGGCACGCACT
GGCGATTCGAGTATTCACCGGAATCCTATACCGGGACCGAGCTGGAATACGCCAAACAGGTGTGCGACGCCGTCGGTGAA
GTCATTCAACCGACACCGGACAACCCGATCATCTTTAACCTGCCTGCCACCGTGGAGATGGCCACCCCCAATGTCTACGC
TGACTCGATCGAGTGGATGAGCCGCAATCTGGCCAACCGAGAGTCCGTCATCCTGAGCCTGCATCCACACAACGACCGCG
GAACCGCTGTCGCCGCAGCCGAATTGGGCTATCACGCCGGTGCCGACAGAATCGAGGGCTGCCTGTTCGGCAACGGGGAA
CGCACCGGCAACGTGTGCCTGGTAACGTTGGGACTGAACCTGTTTTCCCGCGGTGTGGATCCCCAGATCGACTTCTCCAA
TATCGATGAGATCCGGCGAACGGTGGAGTACTGCAACAAGCTGCGGGTGCACGAACGTCACCCCTACGGGGGAGACCTCG
TGTACACCGCGTTCTCTGGCAGCCACCAGGATGCCATCAACAAGGGCCTGGACCAGATGAAGATCGATGCGGACGCCGCC
GACTCCGATGTCGACGACATCCTGTGGCAAGTACCGTACCTGCCGATCGACCCGCGCGACGTCGGCCGCACCTACGAGGC
GGTGATCCGGGTCAACTCGCAGTCTGGTAAGGGCGGCGTGGCTTACATCATGAAGGCCGACCACGGTCTTGAACTGCCGC
GACGGCTGCAGATCGAGTTTTCTCGAGCGATCCAGAAAATCAGTGAGGGCGAAGGAGGCGAGATCACACCCACGGAGATG
TGGGACGTGTTCTTCGAGGAGTACCTCTCACCGGTGCAGCCGCTGGAACGCATAAAGCAGCGGGTCAATGCCGCAGAGGA
AGACGGCGGCAGCACCAGTATTGCCGCTACCGTCAAGATCAACGGTGAAGAGACCGAAATCAGCGGAGTCGGCAACGGCC
CACTGGCCGCTTTCATCGACGCACTGGGCCACGTCGGCTTGCAGGTAGCCGTTCTGGATTATTCCGAACACGCGATGAAC
GCCGGCGACGACGCGCAGGCTGCTGCTTACGTCGAGGCATCGGTACATGGGCACACAGCATGGGGTGTGGGCATTGCACC
GTCGATCACCACAGCGTCGCTACGTGCCGTCGTCTCAGCGGTCAACAGGGCAATGCCCCGCTAG

Upstream 100 bases:

>100_bases
TCGAACGCCGCGACGGGGTCTGATCCCAGACCGGCTGCCCGTTGTGGAAGTTCACTATGCGCCGGTCTGAGGTTCCTTCT
CACATCCCGGAGCAATTATT

Downstream 100 bases:

>100_bases
GACTTTAGTCAGAGACACCGTCCTCGGCGGCACCTCCCCGCCCAAGGCTCCATTTGTCATCGGCGTGCCTGGCCCATCCG
GGCCAGTCGGTCTGCTCGGC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 607; Mature: 606

Protein sequence:

>607_residues
MASFSESLSQDPADAYKSAPSITKPMGPPSPGQPQWNPQRATSMPVFRYRPFAKEVEPIRLVDRTWPDRVIDCAPLWCAV
DLRDGNQALIDPMSPICKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIITDGTIPDDVTIQVLTQCRPELIERTFEA
CENASRVIVHFYNSTSILQRRVVFRADQATVKAIATDGARKCVEEAFKYPGTHWRFEYSPESYTGTELEYAKQVCDAVGE
VIQPTPDNPIIFNLPATVEMATPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGYHAGADRIEGCLFGNGE
RTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNKLRVHERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAA
DSDVDDILWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLELPRRLQIEFSRAIQKISEGEGGEITPTEM
WDVFFEEYLSPVQPLERIKQRVNAAEEDGGSTSIAATVKINGEETEISGVGNGPLAAFIDALGHVGLQVAVLDYSEHAMN
AGDDAQAAAYVEASVHGHTAWGVGIAPSITTASLRAVVSAVNRAMPR

Sequences:

>Translated_607_residues
MASFSESLSQDPADAYKSAPSITKPMGPPSPGQPQWNPQRATSMPVFRYRPFAKEVEPIRLVDRTWPDRVIDCAPLWCAV
DLRDGNQALIDPMSPICKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIITDGTIPDDVTIQVLTQCRPELIERTFEA
CENASRVIVHFYNSTSILQRRVVFRADQATVKAIATDGARKCVEEAFKYPGTHWRFEYSPESYTGTELEYAKQVCDAVGE
VIQPTPDNPIIFNLPATVEMATPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGYHAGADRIEGCLFGNGE
RTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNKLRVHERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAA
DSDVDDILWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLELPRRLQIEFSRAIQKISEGEGGEITPTEM
WDVFFEEYLSPVQPLERIKQRVNAAEEDGGSTSIAATVKINGEETEISGVGNGPLAAFIDALGHVGLQVAVLDYSEHAMN
AGDDAQAAAYVEASVHGHTAWGVGIAPSITTASLRAVVSAVNRAMPR
>Mature_606_residues
ASFSESLSQDPADAYKSAPSITKPMGPPSPGQPQWNPQRATSMPVFRYRPFAKEVEPIRLVDRTWPDRVIDCAPLWCAVD
LRDGNQALIDPMSPICKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIITDGTIPDDVTIQVLTQCRPELIERTFEAC
ENASRVIVHFYNSTSILQRRVVFRADQATVKAIATDGARKCVEEAFKYPGTHWRFEYSPESYTGTELEYAKQVCDAVGEV
IQPTPDNPIIFNLPATVEMATPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGYHAGADRIEGCLFGNGER
TGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNKLRVHERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAAD
SDVDDILWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKADHGLELPRRLQIEFSRAIQKISEGEGGEITPTEMW
DVFFEEYLSPVQPLERIKQRVNAAEEDGGSTSIAATVKINGEETEISGVGNGPLAAFIDALGHVGLQVAVLDYSEHAMNA
GDDAQAAAYVEASVHGHTAWGVGIAPSITTASLRAVVSAVNRAMPR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=532, Percent_Identity=25.5639097744361, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324225, Length=571, Percent_Identity=46.4098073555166, Blast_Score=508, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6324682, Length=576, Percent_Identity=45.8333333333333, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_MYCLE (Q9CB76)

Other databases:

- EMBL:   AL023596
- EMBL:   AL583925
- PIR:   H87199
- RefSeq:   NP_302512.2
- ProteinModelPortal:   Q9CB76
- SMR:   Q9CB76
- EnsemblBacteria:   EBMYCT00000028204
- GeneID:   908681
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2324
- NMPDR:   fig|272631.1.peg.1384
- Leproma:   ML2324
- GeneTree:   EBGT00050000014874
- HOGENOM:   HBG289752
- OMA:   APRWCAV
- ProtClustDB:   PRK03739
- BioCyc:   MLEP272631:ML2324-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_00572
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR10277:SF2
- SMART:   SM00917
- TIGRFAMs:   TIGR00970

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 66899; Mature: 66768

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFSESLSQDPADAYKSAPSITKPMGPPSPGQPQWNPQRATSMPVFRYRPFAKEVEPIR
CCCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHEECCCHHCCCCCE
LVDRTWPDRVIDCAPLWCAVDLRDGNQALIDPMSPICKRRMFDLLVRMGYKEIEVGFPSA
ECCCCCCCHHHCCCCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
SQTDFDFVREIITDGTIPDDVTIQVLTQCRPELIERTFEACENASRVIVHFYNSTSILQR
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHH
RVVFRADQATVKAIATDGARKCVEEAFKYPGTHWRFEYSPESYTGTELEYAKQVCDAVGE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
VIQPTPDNPIIFNLPATVEMATPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAA
HHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEH
ELGYHAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNK
HHCCCCCHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LRVHERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAADSDVDDILWQVPYLPIDPRD
CCCCCCCCCCCCEEEEEECCCCHHHHHCCHHHEEECCCCCCCCHHHHHHHCCCCCCCHHH
VGRTYEAVIRVNSQSGKGGVAYIMKADHGLELPRRLQIEFSRAIQKISEGEGGEITPTEM
HCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
WDVFFEEYLSPVQPLERIKQRVNAAEEDGGSTSIAATVKINGEETEISGVGNGPLAAFID
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHH
ALGHVGLQVAVLDYSEHAMNAGDDAQAAAYVEASVHGHTAWGVGIAPSITTASLRAVVSA
HHHCCCEEEEEEECHHHHCCCCCCCHHEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHH
VNRAMPR
HHHHCCC
>Mature Secondary Structure 
ASFSESLSQDPADAYKSAPSITKPMGPPSPGQPQWNPQRATSMPVFRYRPFAKEVEPIR
CCCHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHEECCCHHCCCCCE
LVDRTWPDRVIDCAPLWCAVDLRDGNQALIDPMSPICKRRMFDLLVRMGYKEIEVGFPSA
ECCCCCCCHHHCCCCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCC
SQTDFDFVREIITDGTIPDDVTIQVLTQCRPELIERTFEACENASRVIVHFYNSTSILQR
CCCCHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCHHHHHH
RVVFRADQATVKAIATDGARKCVEEAFKYPGTHWRFEYSPESYTGTELEYAKQVCDAVGE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
VIQPTPDNPIIFNLPATVEMATPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAA
HHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEH
ELGYHAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNK
HHCCCCCHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LRVHERHPYGGDLVYTAFSGSHQDAINKGLDQMKIDADAADSDVDDILWQVPYLPIDPRD
CCCCCCCCCCCCEEEEEECCCCHHHHHCCHHHEEECCCCCCCCHHHHHHHCCCCCCCHHH
VGRTYEAVIRVNSQSGKGGVAYIMKADHGLELPRRLQIEFSRAIQKISEGEGGEITPTEM
HCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
WDVFFEEYLSPVQPLERIKQRVNAAEEDGGSTSIAATVKINGEETEISGVGNGPLAAFID
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHH
ALGHVGLQVAVLDYSEHAMNAGDDAQAAAYVEASVHGHTAWGVGIAPSITTASLRAVVSA
HHHCCCEEEEEEECHHHHCCCCCCCHHEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHH
VNRAMPR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002