Click here to switch to the map view.

The map label for this gene is menD

Identifier: 221230692

GI number: 221230692

Start: 2693730

End: 2695400

Strand: Direct

Name: menD

Synonym: MLBr_02270

Alternate gene names: 221230692

Gene position: 2693730-2695400 (Clockwise)

Preceding gene: 221230691

Following gene: 221230693

Centisome position: 82.43

GC content: 64.93

Gene sequence:

>1671_bases
ATGAACCCCTCGACGACACAGGCTCGCGTCGTCGTTGACGAGCTGATCCGCGGCGGCGTCCGCGATGTAGTGCTGTGCCC
CGGGTCGCGAAACGCCCCGTTGGCATTCGCCCTGCAAGACGCCGACCACGCCGGCCGGATACGGCTGCACGTACGCATTG
ACGAGCGCACCGCCGGGTATCTGGCTATCGGGCTGGCGGTCGCCGCGGGCGCGCCGGTGTGTGTGGCGATGACCTCGGGC
ACTGCCGTCGCCAACCTTGGCCCAGCGGTGGTCGAAGCCAACTATGCCCGGGTGCCACTGATCGTGTTATCGGCCAACCG
GCCTTATGAATTGCTCGGCACAGGGGCCAACCAGACCATGGAGCAGCTGGGCTACTTCGGCACCCAAGTTCGTGCCGCCA
TCAGCCTAGGCCTAGCAGAGGACGCGCCGGAGCGGCTCGATTCACTCAACGCAACCTGGCGATCGGCCACCTGCCGAGTA
TTGGTGGCTGCCACCGGTTCTCGTACCGCCAACGCCGGCCCCGTGCAGTTCGACATCCCACTGCGCGAGCCTTTAATACC
CGACCCGGAACCCCACGGCGCCATCACACCGCAGGGCCGGCCCGGCGGAAAGCCGTGGACCTACATACCGCAGGTGACGT
TCGACCAGCCGCTGGAAATCGACTTGTCAGCCGACACCGTCGTCATCGCCGGGCACGGTGCAGGCGTGCACCCGAACCTG
ACCGAATTGCCGATCGTTGCTGAGCCGACCGCGCCATTTGGACCGTACCGGCCCTACCCGTTGCACCCCCTAGCGCTGCC
TCTGCTGCACCCGAAGCAGGTGATCATGCTCGGTCGCCCGACACTGCACCGCCCGGTGTCGGCGCTACTGGCCGACCCAC
AAGTGCCGGTGTACGCGCTTACCACTGGGCCGTGTTGGCCGGACGTCTCGGGCAATTCTCAGGCCGCTGGTACTCGGGCG
GTAACCACCGGGACACCGCATCCGGCCTGGCTGCAGCGCTGTGCGGAGATGAACCAGCACGCGATCTCGACGGTGCGCAG
CCAGCTCGCGGCTCATCCGTTAATCACGGGTTTGCATGTCGCTGCAGCTGTGGCAGATGCACTACGGCCCGGTGACCAAC
TGGTGCTTGGGGCGTCCAACCCGGTGCGCGACATGGCGTTGGTTGGTTTGTCCACTGACGGTATTCAGGTGCGGTCCAAC
CGCGGCGTCGCTGGCATCGACGGCACCGTATCCACCGCGATCGGGGCGGCGCTAGCATATGAACGATCCCCCGGCTACGC
TGGTAGCACAGACCGCCCGGCTCGTACCGTAGCATTAATCGGAGACCTCGCATTCGTCCATGACAGCTCGGGGCTGTTGA
TCGGCCCCACCGAACCCACACCGCAGCAGCTGAAAATCGTAGTGTCTAACGACAACGGTGGGGGTATCTTCGAACTGCTG
GAGCAAGGCGACCCGAGGTTGTCTGCCGTATCGTCGCGCATCTTCGGTACTCCGCACGACGTCGACGTAGGAGCGTTGTG
TCGCGCGTACCACGTGGAAGGTCGACAGATCGAGGTCGACAAGCTGCGGGAAGCCCTCGACGAACCCGGTGTTGGCATGC
GCGTGCTGGAAGTCAAGGCCGACCGATCTTCGCTGCGCCAGTTGCATGCAGCAATCACGGCAACATTGTGA

Upstream 100 bases:

>100_bases
CGGTCCGGATCACCCCGTCGTCAAGCCCCTCGCTATGAGGGAAGAGCTTTAGCTAAGCCGCGCCGGCCGCATTGTTGCCG
GGCTATGACACCCTGTACGG

Downstream 100 bases:

>100_bases
GGTTGCTATTATGAGGTATTGTCAACTGTGATATTGTCGAAGTCGTTGCTGCGCATCCTTATTCATGGTCGCAGCGACGA
ACCGCCCAGTACTCGTGCCA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADHAGRIRLHVRIDERTAGYLAIGLAVAAGAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRAAISLGLAEDAPERLDSLNATWRSATCRV
LVAATGSRTANAGPVQFDIPLREPLIPDPEPHGAITPQGRPGGKPWTYIPQVTFDQPLEIDLSADTVVIAGHGAGVHPNL
TELPIVAEPTAPFGPYRPYPLHPLALPLLHPKQVIMLGRPTLHRPVSALLADPQVPVYALTTGPCWPDVSGNSQAAGTRA
VTTGTPHPAWLQRCAEMNQHAISTVRSQLAAHPLITGLHVAAAVADALRPGDQLVLGASNPVRDMALVGLSTDGIQVRSN
RGVAGIDGTVSTAIGAALAYERSPGYAGSTDRPARTVALIGDLAFVHDSSGLLIGPTEPTPQQLKIVVSNDNGGGIFELL
EQGDPRLSAVSSRIFGTPHDVDVGALCRAYHVEGRQIEVDKLREALDEPGVGMRVLEVKADRSSLRQLHAAITATL

Sequences:

>Translated_556_residues
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADHAGRIRLHVRIDERTAGYLAIGLAVAAGAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRAAISLGLAEDAPERLDSLNATWRSATCRV
LVAATGSRTANAGPVQFDIPLREPLIPDPEPHGAITPQGRPGGKPWTYIPQVTFDQPLEIDLSADTVVIAGHGAGVHPNL
TELPIVAEPTAPFGPYRPYPLHPLALPLLHPKQVIMLGRPTLHRPVSALLADPQVPVYALTTGPCWPDVSGNSQAAGTRA
VTTGTPHPAWLQRCAEMNQHAISTVRSQLAAHPLITGLHVAAAVADALRPGDQLVLGASNPVRDMALVGLSTDGIQVRSN
RGVAGIDGTVSTAIGAALAYERSPGYAGSTDRPARTVALIGDLAFVHDSSGLLIGPTEPTPQQLKIVVSNDNGGGIFELL
EQGDPRLSAVSSRIFGTPHDVDVGALCRAYHVEGRQIEVDKLREALDEPGVGMRVLEVKADRSSLRQLHAAITATL
>Mature_556_residues
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADHAGRIRLHVRIDERTAGYLAIGLAVAAGAPVCVAMTSG
TAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTMEQLGYFGTQVRAAISLGLAEDAPERLDSLNATWRSATCRV
LVAATGSRTANAGPVQFDIPLREPLIPDPEPHGAITPQGRPGGKPWTYIPQVTFDQPLEIDLSADTVVIAGHGAGVHPNL
TELPIVAEPTAPFGPYRPYPLHPLALPLLHPKQVIMLGRPTLHRPVSALLADPQVPVYALTTGPCWPDVSGNSQAAGTRA
VTTGTPHPAWLQRCAEMNQHAISTVRSQLAAHPLITGLHVAAAVADALRPGDQLVLGASNPVRDMALVGLSTDGIQVRSN
RGVAGIDGTVSTAIGAALAYERSPGYAGSTDRPARTVALIGDLAFVHDSSGLLIGPTEPTPQQLKIVVSNDNGGGIFELL
EQGDPRLSAVSSRIFGTPHDVDVGALCRAYHVEGRQIEVDKLREALDEPGVGMRVLEVKADRSSLRQLHAAITATL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=525, Percent_Identity=29.9047619047619, Blast_Score=146, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_MYCLB (B8ZSY2)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002504108.1
- ProteinModelPortal:   B8ZSY2
- EnsemblBacteria:   EBMYCT00000086528
- GeneID:   7324873
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015760
- HOGENOM:   HBG634405
- OMA:   IAEPTAP
- ProtClustDB:   PRK07449
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 58351; Mature: 58351

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADHAGRIRLHVRIDERTAGY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCE
LAIGLAVAAGAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTM
EEEEHHHHCCCCEEEEEECCCEEECCCCHHEECCCCEEEEEEEECCCCCEEEECCCHHHH
EQLGYFGTQVRAAISLGLAEDAPERLDSLNATWRSATCRVLVAATGSRTANAGPVQFDIP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEEEECC
LREPLIPDPEPHGAITPQGRPGGKPWTYIPQVTFDQPLEIDLSADTVVIAGHGAGVHPNL
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCC
TELPIVAEPTAPFGPYRPYPLHPLALPLLHPKQVIMLGRPTLHRPVSALLADPQVPVYAL
CCCCEEECCCCCCCCCCCCCCCHHEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEE
TTGPCWPDVSGNSQAAGTRAVTTGTPHPAWLQRCAEMNQHAISTVRSQLAAHPLITGLHV
ECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AAAVADALRPGDQLVLGASNPVRDMALVGLSTDGIQVRSNRGVAGIDGTVSTAIGAALAY
HHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCEEEECCCCEECCCCHHHHHHHHHHEE
ERSPGYAGSTDRPARTVALIGDLAFVHDSSGLLIGPTEPTPQQLKIVVSNDNGGGIFELL
ECCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEECCCCCCCCEEEEEEECCCCCHHHHH
EQGDPRLSAVSSRIFGTPHDVDVGALCRAYHVEGRQIEVDKLREALDEPGVGMRVLEVKA
HCCCCHHHHHHHHHCCCCCCCCHHHHHHHEECCCCEEEHHHHHHHHCCCCCCEEEEEEEC
DRSSLRQLHAAITATL
CHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPSTTQARVVVDELIRGGVRDVVLCPGSRNAPLAFALQDADHAGRIRLHVRIDERTAGY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCCE
LAIGLAVAAGAPVCVAMTSGTAVANLGPAVVEANYARVPLIVLSANRPYELLGTGANQTM
EEEEHHHHCCCCEEEEEECCCEEECCCCHHEECCCCEEEEEEEECCCCCEEEECCCHHHH
EQLGYFGTQVRAAISLGLAEDAPERLDSLNATWRSATCRVLVAATGSRTANAGPVQFDIP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEEEECC
LREPLIPDPEPHGAITPQGRPGGKPWTYIPQVTFDQPLEIDLSADTVVIAGHGAGVHPNL
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEECCCEEEEEECCCCCCCCC
TELPIVAEPTAPFGPYRPYPLHPLALPLLHPKQVIMLGRPTLHRPVSALLADPQVPVYAL
CCCCEEECCCCCCCCCCCCCCCHHEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEE
TTGPCWPDVSGNSQAAGTRAVTTGTPHPAWLQRCAEMNQHAISTVRSQLAAHPLITGLHV
ECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
AAAVADALRPGDQLVLGASNPVRDMALVGLSTDGIQVRSNRGVAGIDGTVSTAIGAALAY
HHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCEEEECCCCEECCCCHHHHHHHHHHEE
ERSPGYAGSTDRPARTVALIGDLAFVHDSSGLLIGPTEPTPQQLKIVVSNDNGGGIFELL
ECCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEECCCCCCCCEEEEEEECCCCCHHHHH
EQGDPRLSAVSSRIFGTPHDVDVGALCRAYHVEGRQIEVDKLREALDEPGVGMRVLEVKA
HCCCCHHHHHHHHHCCCCCCCCHHHHHHHEECCCCEEEHHHHHHHHCCCCCCEEEEEEEC
DRSSLRQLHAAITATL
CHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA