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The map label for this gene is pitA

Identifier: 221230685

GI number: 221230685

Start: 2683072

End: 2684316

Strand: Reverse

Name: pitA

Synonym: MLBr_02260

Alternate gene names: 221230685

Gene position: 2684316-2683072 (Counterclockwise)

Preceding gene: 221230686

Following gene: 221230684

Centisome position: 82.14

GC content: 59.68

Gene sequence:

>1245_bases
GTGAACATCAATTTGTTCCTCTTGATCATTGTCGTGATCACGGCACTGGCCTTCGACTTCACCAACGGTTTTCACGACAC
CGGGAACGCCATGGCGACCTCGATCGCCAGTGGTGCGCTCGCACCAAAGGTGGCGGTGTTCTTCTCTGCCATTTTGAACC
TGGTCGGCGCGTTCTTGTCTACCGCAGTCGCAGCCACGATTGCCAAGGATCTGATCGAGGCGGATCTGGTAACGCTGGAA
CTGGTGTTCGCCGGCCTGGTCGGCGGTATCGTCTGGAATTTGCTGACCTGGCTTCTCGGTATCCCGTCGAGTTCCTCGCA
CGCACTGATTGGCGGTATCGTCGGCGCCAGGATCGCTGCTGTCGGCGGTCACGGGGTGATCTGGAGCGGTGTCATATCCA
AGGTGATTATTCCGGCCATTATTGCCGCGTTGCTGGCCATCGTTGTTGGGGCGGTGGCCACCTGGTTGGTCTACGCGATC
ACTCGCAGTGTTCCAGCTATGAGCACCGACACCAGGTTTCGGCGCGGCCAGATCGGCTCGGCGTCGCTAGTTTCACTTGC
ACACGGGACCAACGACGCACAGAAGACGATGGGCGTGATCTTCCTGGCTCTGATGTCCTATGGAACAGTCAGCAAGACCG
CTTCCACGCCGCCGCTGTGGGTGATCGTGTGTTGCGCTATAGCCATTGCCGCGGGTACCTACCTGGGCGGCTGGCGAATC
ATCCGCACTTTGGGCAAGGGGATGGTGGAGATCAAGCCACCGCAAGGAATGGCCGCCGAATCATCCTCGGCCGCGGTCAT
TCTGTTGTCCGCACACTTTGGTTACGCGTTGTCCACGACCCAAGTCTGCACTGGTTCGGTGCTGGGCAGCGGGTTGGGCA
AACCCGGCGGCGAGGTTCGGTGGGGCGTGGCTGGTCGGATGGCCACCGCGTGGCTGGTCACGCTTCCGTTGGCTGGTTCG
GTGGGAGCAGTCACCTACTGGATCGTGCATCTAATCGGTGGTTATCCCGGCGCGGTCATAGGGTTCTCGCTGTTGGTCGC
GGCCTCTGTCGCCATCTACATCCGGTCGCGTAAGGTCAAGGTCGACCACAAGAACGTCAATGAAAACTGGGAAGGCAGCT
TGACCGCTGGACTCGACGGTTCGGACGAACATAAGCCACACTCTGATGTGGGCCCTAAATTGAGTGCTACCCTGCCTCGC
TACCGCTCTAGTCACCACACGGTTGGCGTAAGGAACGCCTCTTGA

Upstream 100 bases:

>100_bases
TAATTGCACGGTAACATTTGGCGCTGGGTCGCAGCTTACCTGCGATTCATGCAACAGTTAATTCGACAATGGATTGCCCA
GACCACGAGAATCAGGCGCT

Downstream 100 bases:

>100_bases
GCCCTTGGTTCAACTACGAGGCCACCCTCAAGATCCTTCTTTTCAGCACGCTGGCCGGTGCGGCATTGCCGGGGCTGTTC
GCGCTCGGGATCCGGCTGCA

Product: low-affinity inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MNINLFLLIIVVITALAFDFTNGFHDTGNAMATSIASGALAPKVAVFFSAILNLVGAFLSTAVAATIAKDLIEADLVTLE
LVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGARIAAVGGHGVIWSGVISKVIIPAIIAALLAIVVGAVATWLVYAI
TRSVPAMSTDTRFRRGQIGSASLVSLAHGTNDAQKTMGVIFLALMSYGTVSKTASTPPLWVIVCCAIAIAAGTYLGGWRI
IRTLGKGMVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVCTGSVLGSGLGKPGGEVRWGVAGRMATAWLVTLPLAGS
VGAVTYWIVHLIGGYPGAVIGFSLLVAASVAIYIRSRKVKVDHKNVNENWEGSLTAGLDGSDEHKPHSDVGPKLSATLPR
YRSSHHTVGVRNAS

Sequences:

>Translated_414_residues
MNINLFLLIIVVITALAFDFTNGFHDTGNAMATSIASGALAPKVAVFFSAILNLVGAFLSTAVAATIAKDLIEADLVTLE
LVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGARIAAVGGHGVIWSGVISKVIIPAIIAALLAIVVGAVATWLVYAI
TRSVPAMSTDTRFRRGQIGSASLVSLAHGTNDAQKTMGVIFLALMSYGTVSKTASTPPLWVIVCCAIAIAAGTYLGGWRI
IRTLGKGMVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVCTGSVLGSGLGKPGGEVRWGVAGRMATAWLVTLPLAGS
VGAVTYWIVHLIGGYPGAVIGFSLLVAASVAIYIRSRKVKVDHKNVNENWEGSLTAGLDGSDEHKPHSDVGPKLSATLPR
YRSSHHTVGVRNAS
>Mature_414_residues
MNINLFLLIIVVITALAFDFTNGFHDTGNAMATSIASGALAPKVAVFFSAILNLVGAFLSTAVAATIAKDLIEADLVTLE
LVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGARIAAVGGHGVIWSGVISKVIIPAIIAALLAIVVGAVATWLVYAI
TRSVPAMSTDTRFRRGQIGSASLVSLAHGTNDAQKTMGVIFLALMSYGTVSKTASTPPLWVIVCCAIAIAAGTYLGGWRI
IRTLGKGMVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVCTGSVLGSGLGKPGGEVRWGVAGRMATAWLVTLPLAGS
VGAVTYWIVHLIGGYPGAVIGFSLLVAASVAIYIRSRKVKVDHKNVNENWEGSLTAGLDGSDEHKPHSDVGPKLSATLPR
YRSSHHTVGVRNAS

Specific function: Low-affinity inorganic phosphate transport

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily

Homologues:

Organism=Homo sapiens, GI31543630, Length=153, Percent_Identity=32.0261437908497, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI5803173, Length=149, Percent_Identity=32.2147651006711, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789360, Length=219, Percent_Identity=30.1369863013699, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789907, Length=222, Percent_Identity=29.7297297297297, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=170, Percent_Identity=31.7647058823529, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17539280, Length=157, Percent_Identity=29.9363057324841, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32566716, Length=179, Percent_Identity=31.8435754189944, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25146401, Length=171, Percent_Identity=29.8245614035088, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536725, Length=188, Percent_Identity=24.468085106383, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=150, Percent_Identity=28.6666666666667, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356511, Length=180, Percent_Identity=28.3333333333333, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIT_MYCLE (Q50173)

Other databases:

- EMBL:   U15187
- EMBL:   AL583924
- PIR:   H87191
- RefSeq:   NP_302471.1
- ProteinModelPortal:   Q50173
- EnsemblBacteria:   EBMYCT00000028988
- GeneID:   908765
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2260
- NMPDR:   fig|272631.1.peg.1343
- Leproma:   ML2260
- GeneTree:   EBGT00050000017317
- HOGENOM:   HBG681698
- OMA:   SCALAIS
- ProtClustDB:   CLSK871824
- BioCyc:   MLEP272631:ML2260-MONOMER
- InterPro:   IPR001204
- PANTHER:   PTHR11101

Pfam domain/function: PF01384 PHO4

EC number: NA

Molecular weight: Translated: 42728; Mature: 42728

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x236416f0)-; HASH(0x21defdb8)-; HASH(0x451e0a44)-; HASH(0x451e195c)-; HASH(0x451e08d0)-; HASH(0x21865b8c)-; HASH(0x2187014c)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINLFLLIIVVITALAFDFTNGFHDTGNAMATSIASGALAPKVAVFFSAILNLVGAFLS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TAVAATIAKDLIEADLVTLELVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGARIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VGGHGVIWSGVISKVIIPAIIAALLAIVVGAVATWLVYAITRSVPAMSTDTRFRRGQIGS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
ASLVSLAHGTNDAQKTMGVIFLALMSYGTVSKTASTPPLWVIVCCAIAIAAGTYLGGWRI
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IRTLGKGMVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVCTGSVLGSGLGKPGGEVR
HHHHCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEE
WGVAGRMATAWLVTLPLAGSVGAVTYWIVHLIGGYPGAVIGFSLLVAASVAIYIRSRKVK
ECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCEEE
VDHKNVNENWEGSLTAGLDGSDEHKPHSDVGPKLSATLPRYRSSHHTVGVRNAS
ECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCHHHCCCCCEEEECCCH
>Mature Secondary Structure
MNINLFLLIIVVITALAFDFTNGFHDTGNAMATSIASGALAPKVAVFFSAILNLVGAFLS
CCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TAVAATIAKDLIEADLVTLELVFAGLVGGIVWNLLTWLLGIPSSSSHALIGGIVGARIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VGGHGVIWSGVISKVIIPAIIAALLAIVVGAVATWLVYAITRSVPAMSTDTRFRRGQIGS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
ASLVSLAHGTNDAQKTMGVIFLALMSYGTVSKTASTPPLWVIVCCAIAIAAGTYLGGWRI
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IRTLGKGMVEIKPPQGMAAESSSAAVILLSAHFGYALSTTQVCTGSVLGSGLGKPGGEVR
HHHHCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEE
WGVAGRMATAWLVTLPLAGSVGAVTYWIVHLIGGYPGAVIGFSLLVAASVAIYIRSRKVK
ECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCEEE
VDHKNVNENWEGSLTAGLDGSDEHKPHSDVGPKLSATLPRYRSSHHTVGVRNAS
ECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCHHHCCCCCEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002