Click here to switch to the map view.

The map label for this gene is purF

Identifier: 221230661

GI number: 221230661

Start: 2620540

End: 2622210

Strand: Reverse

Name: purF

Synonym: MLBr_02206

Alternate gene names: 221230661

Gene position: 2622210-2620540 (Counterclockwise)

Preceding gene: 221230662

Following gene: 221230660

Centisome position: 80.24

GC content: 62.0

Gene sequence:

>1671_bases
GTGTGTTTGGCTGTCGGGGTAGGTGTCCGGGCACCTAAACACGTGCCACAGATTCGTCGACTGGGCCGTGCTGGCCGTAG
ACTCCGTTGCGTCACCAACTGCGCCCTAGGGAGCTGCCCAATCGTGACCGTCCAGCAACCCGGGCGGGATTTCAGCTCGC
CCCGCGAAGAGTGCGGTGTGTTCGGGGTCTGGGCTCCGGGTGAACTAGTCGCCAAACTCACTTATTTCGGTCTATACGCG
TTGCAGCATCGCGGCCAGGAAGCTGCGGGGATCGCTGTCGCTGATGGATCGCAGGTGTTGGTCTTCAAAGACCTTGGCTT
AGTTAGTCAAGTGTTCGACGAGCAGACATTGGCGGCGATGGAGGGTCACGTTGCCATCGGTCATTGCCGCTACTCCACCA
CCGGCGACACCACCTGGGAAAATGCCCAGCCTGTTTTCCGCAACATCGCTGCCGGATCCGGGGTTGCGTTGGGGCACAAC
GGAAACCTGGTCAACACTGCCGAGCTTGCCGCACGTGCCCGCGACGCGGGGTTGATAGCAAAACGCTGCCCGGCCCCAGC
GACAACGGACTCCGACATCCTGGGCGCGCTGTTAGCCCACGGTGCGGCCGACTCGACCCTGGAACAGGCTGCGCTGGAAC
TGCTGCCGACCGTGCGGGGTGCGTTCTGCCTGACATTCATGGACGAAAACACCCTCTATGCCTGCCGGGACCCGTATGGA
GTACGGCCGCTGTCGCTCGGACGACTGGATCGCGGCTGGGTGGTGGCTTCTGAAACGGCGGGGCTCGACATTGTCGGCGC
CTCGTTTGTCCGCGACATCGAGCCGGGCGAATTGCTGGCGATCGACGCCGACGGAGTACGGTCCACTCGTTTCGCCAACC
CTACGCCCAAGGGTTGTGTTTTTGAGTACGTTTACCTGGCACGGCCCGACAGCACGCTGGCCGGACGGTCGGTGCACGGC
ACTCGGGTGGAGATCGGTCGTCGACTTGCCCGCGAATGCCCGGTCGAGGCCGACTTGGTGATCGGTGTGCCCGAATCAGG
CACTCCCGCCGCAGTGGGATACGCGCAGGAATCCGGCATCTCTTACGGGCAGGGTCTGATGAAGAATGCATATGTTGGAC
GCACATTCATCCAACCGTCGCAAACCATCCGTCAACTGGGCATCCGGCTGAAGCTCAATCCGCTTAAAGAGGTAATCCGC
GGCAAGCGGCTGATTGTTGTCGACGACTCGGTTGTGCGGGGGAACACCCAACGCGCTCTGGTGCGGATGCTGCGCGAGGC
CGGTGCCGTCGAGCTGCACGTGCGCATCGCTTCACCACCGGTGAAGTGGCCCTGTTTTTACGGCATCGACTTCCCATCGC
CGGCCGAATTGATCGCTAACGTCGTCGCAGACGAGGAGGAGATGCTCGAGGCGGTACGGCAGGGCATCGGTGCCGACACA
CTGGGCTACATCTCGCTGCGGGGCATGATTGCGGCTTCCGAGCAGCCCGCTTCGCGGCTGTGTTACGCTTGCTTTGACGG
TAGGTATCCGATCGAGCTGCCCAGTGAAGCCATGTTAGGCAAAAATGTCATCGAGCACATGCTTGCCAATGCGGCGCGTG
GAGCCGGGCTACGCGATCTTGCTGCTGATCAGGTCCCAGTCGATGCGGACGAAAATTGCGTTTGGCGTTGA

Upstream 100 bases:

>100_bases
CGGTGGCGACCGGTGCGCTGCGTATGTCTGGCTCGCGTGCCGGTGACATCGAGGTGTGGATGCCATTAATCAATCTACGC
TGTACGTAGTTGGCTAAACT

Downstream 100 bases:

>100_bases
CACGATGTGAGTCCCGGCAGCACGGGGTGCTTGGTGGGCAACCGTTCAAATGTAGTACCTGATTCCGACTGTGAGTGACG
ACCGGTAGCCTTTATCGCGA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MCLAVGVGVRAPKHVPQIRRLGRAGRRLRCVTNCALGSCPIVTVQQPGRDFSSPREECGVFGVWAPGELVAKLTYFGLYA
LQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMEGHVAIGHCRYSTTGDTTWENAQPVFRNIAAGSGVALGHN
GNLVNTAELAARARDAGLIAKRCPAPATTDSDILGALLAHGAADSTLEQAALELLPTVRGAFCLTFMDENTLYACRDPYG
VRPLSLGRLDRGWVVASETAGLDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTLAGRSVHG
TRVEIGRRLARECPVEADLVIGVPESGTPAAVGYAQESGISYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIR
GKRLIVVDDSVVRGNTQRALVRMLREAGAVELHVRIASPPVKWPCFYGIDFPSPAELIANVVADEEEMLEAVRQGIGADT
LGYISLRGMIAASEQPASRLCYACFDGRYPIELPSEAMLGKNVIEHMLANAARGAGLRDLAADQVPVDADENCVWR

Sequences:

>Translated_556_residues
MCLAVGVGVRAPKHVPQIRRLGRAGRRLRCVTNCALGSCPIVTVQQPGRDFSSPREECGVFGVWAPGELVAKLTYFGLYA
LQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMEGHVAIGHCRYSTTGDTTWENAQPVFRNIAAGSGVALGHN
GNLVNTAELAARARDAGLIAKRCPAPATTDSDILGALLAHGAADSTLEQAALELLPTVRGAFCLTFMDENTLYACRDPYG
VRPLSLGRLDRGWVVASETAGLDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTLAGRSVHG
TRVEIGRRLARECPVEADLVIGVPESGTPAAVGYAQESGISYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIR
GKRLIVVDDSVVRGNTQRALVRMLREAGAVELHVRIASPPVKWPCFYGIDFPSPAELIANVVADEEEMLEAVRQGIGADT
LGYISLRGMIAASEQPASRLCYACFDGRYPIELPSEAMLGKNVIEHMLANAARGAGLRDLAADQVPVDADENCVWR
>Mature_556_residues
MCLAVGVGVRAPKHVPQIRRLGRAGRRLRCVTNCALGSCPIVTVQQPGRDFSSPREECGVFGVWAPGELVAKLTYFGLYA
LQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAMEGHVAIGHCRYSTTGDTTWENAQPVFRNIAAGSGVALGHN
GNLVNTAELAARARDAGLIAKRCPAPATTDSDILGALLAHGAADSTLEQAALELLPTVRGAFCLTFMDENTLYACRDPYG
VRPLSLGRLDRGWVVASETAGLDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCVFEYVYLARPDSTLAGRSVHG
TRVEIGRRLARECPVEADLVIGVPESGTPAAVGYAQESGISYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIR
GKRLIVVDDSVVRGNTQRALVRMLREAGAVELHVRIASPPVKWPCFYGIDFPSPAELIANVVADEEEMLEAVRQGIGADT
LGYISLRGMIAASEQPASRLCYACFDGRYPIELPSEAMLGKNVIEHMLANAARGAGLRDLAADQVPVDADENCVWR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI29570798, Length=519, Percent_Identity=39.1136801541426, Blast_Score=348, Evalue=1e-95,
Organism=Escherichia coli, GI1788651, Length=479, Percent_Identity=38.6221294363257, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17554892, Length=491, Percent_Identity=37.8818737270876, Blast_Score=308, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6323958, Length=461, Percent_Identity=35.1409978308026, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24659598, Length=516, Percent_Identity=38.953488372093, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI28573187, Length=515, Percent_Identity=40.1941747572816, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=39.5397489539749, Blast_Score=340, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR1_MYCLE (Q50028)

Other databases:

- EMBL:   U15182
- EMBL:   Z95151
- EMBL:   AL583924
- PIR:   A87185
- RefSeq:   NP_302447.1
- ProteinModelPortal:   Q50028
- SMR:   Q50028
- MEROPS:   C44.001
- EnsemblBacteria:   EBMYCT00000029429
- GeneID:   908827
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2206
- NMPDR:   fig|272631.1.peg.1319
- Leproma:   ML2206
- GeneTree:   EBGT00050000016850
- HOGENOM:   HBG392416
- OMA:   VWAPGEE
- ProtClustDB:   PRK07847
- BioCyc:   MLEP272631:ML2206-MONOMER
- BRENDA:   2.4.2.14
- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836
- PANTHER:   PTHR11907
- PIRSF:   PIRSF000485
- TIGRFAMs:   TIGR01134

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran

EC number: =2.4.2.14

Molecular weight: Translated: 59541; Mature: 59541

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS51278 GATASE_TYPE_2; PS00103 PUR_PYR_PR_TRANSFER

Important sites: ACT_SITE 58-58

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLAVGVGVRAPKHVPQIRRLGRAGRRLRCVTNCALGSCPIVTVQQPGRDFSSPREECGV
CEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCCCHHHCCE
FGVWAPGELVAKLTYFGLYALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAM
EEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHH
EGHVAIGHCRYSTTGDTTWENAQPVFRNIAAGSGVALGHNGNLVNTAELAARARDAGLIA
CCCEEEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCCCHH
KRCPAPATTDSDILGALLAHGAADSTLEQAALELLPTVRGAFCLTFMDENTLYACRDPYG
HCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCC
VRPLSLGRLDRGWVVASETAGLDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCV
CCCCCCCCCCCCEEEEECCCCCEEECHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCE
FEYVYLARPDSTLAGRSVHGTRVEIGRRLARECPVEADLVIGVPESGTPAAVGYAQESGI
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECHHHCCC
SYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSVVRGNTQRAL
CHHHHHHHCCCCCCEECCHHHHHHHCCCEEEECHHHHHHCCCEEEEECCHHHCCCHHHHH
VRMLREAGAVELHVRIASPPVKWPCFYGIDFPSPAELIANVVADEEEMLEAVRQGIGADT
HHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCC
LGYISLRGMIAASEQPASRLCYACFDGRYPIELPSEAMLGKNVIEHMLANAARGAGLRDL
CCEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
AADQVPVDADENCVWR
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCLAVGVGVRAPKHVPQIRRLGRAGRRLRCVTNCALGSCPIVTVQQPGRDFSSPREECGV
CEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCCCCCHHHCCE
FGVWAPGELVAKLTYFGLYALQHRGQEAAGIAVADGSQVLVFKDLGLVSQVFDEQTLAAM
EEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECHHHHHHHHHHHHHHHH
EGHVAIGHCRYSTTGDTTWENAQPVFRNIAAGSGVALGHNGNLVNTAELAARARDAGLIA
CCCEEEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCCCHH
KRCPAPATTDSDILGALLAHGAADSTLEQAALELLPTVRGAFCLTFMDENTLYACRDPYG
HCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCC
VRPLSLGRLDRGWVVASETAGLDIVGASFVRDIEPGELLAIDADGVRSTRFANPTPKGCV
CCCCCCCCCCCCEEEEECCCCCEEECHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCE
FEYVYLARPDSTLAGRSVHGTRVEIGRRLARECPVEADLVIGVPESGTPAAVGYAQESGI
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECHHHCCC
SYGQGLMKNAYVGRTFIQPSQTIRQLGIRLKLNPLKEVIRGKRLIVVDDSVVRGNTQRAL
CHHHHHHHCCCCCCEECCHHHHHHHCCCEEEECHHHHHHCCCEEEEECCHHHCCCHHHHH
VRMLREAGAVELHVRIASPPVKWPCFYGIDFPSPAELIANVVADEEEMLEAVRQGIGADT
HHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCC
LGYISLRGMIAASEQPASRLCYACFDGRYPIELPSEAMLGKNVIEHMLANAARGAGLRDL
CCEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
AADQVPVDADENCVWR
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002