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The map label for this gene is cysA3

Identifier: 221230654

GI number: 221230654

Start: 2611986

End: 2612819

Strand: Direct

Name: cysA3

Synonym: MLBr_02198

Alternate gene names: 221230654

Gene position: 2611986-2612819 (Clockwise)

Preceding gene: 221230653

Following gene: 221230655

Centisome position: 79.92

GC content: 56.47

Gene sequence:

>834_bases
ATGGCACGCTCTGATGTCCTGGTCTCTGCCGACTGGGCCGAGAGCAACCTCGACAGCGCGAACATCGTCTTCGTCGAAGT
GGACGAGGACACAAGCACCTACGACGGCGATCACATCGCCGGCGCCATCAAGTTGGATTGGCGTGCGGATTTGCAAGACC
CGATCAAGCGCGACTTCATCGACACCCAGCAATTCTCCAAATTGTTGGGTGACCGAGGCATTTCCAATGACAACACCGTG
ATTCTGTACGGCGGCAACAACAACTGGTTCGCAGCCTATGCGTATTGGTATTTCAAGCTGTACAGGCATGACAAAGTCAA
ATTGCTTGACGGTGGCCGCAAGAAGTGGGAGCTCGACGGTCGCCCGCTGTCCACCGACACCGTCACCAGGCCGGCGACCT
CCTACGCTGCTGCAGCACCGGACAACACTATCCGGGCCTTTCGGGACGAGGTCATAGCATCCATCAAGATCAAAAATCTC
GTCGACGTACGTTCCCCCGACGAATTCTCCGGAAAGCTCCTGGCACCTGCGCACCTACCTCAGGAGCAGAGCCAGCGACC
CGGCCACATCCCCAGTGCGATAAACATACCTTGGAGCAAGGCGGCCAATGAGGACGGCACCTTCAAATCCGACGAGCAGT
TAGCCAAGCTGTATGCCGACGCCGGCCTGGATAGGTTGAAAGAGACAATCGTCTACTGCCGAATCGGTGAACGATCATCA
CACACCTGGTTCGTGCTGCGCGAGTTACTCGGATATCAAAACGTCAAGAACTACGACGGCAGTTGGACAGAATACGGCTC
CCTGGTGGGTGTCCCAATCGAATTGGGAAGCTGA

Upstream 100 bases:

>100_bases
TGTCAGCTCTACCCGGTTGTGGTGTGGTTTCGACGCACTGCCGCTCCACGTAACCGTAACCCTGGTTGACCACCCCAAGG
TAAGTGAAAGGACTCTAACC

Downstream 100 bases:

>100_bases
CATGTGCTCTGAGCCTAAACAAAGACTGGCGTTGCCCGCCAACGTGAACCTCGAGAAAGAAACGGTGATCACCGGTCGCG
TGGTCGATCGCAAAGGGCAA

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MARSDVLVSADWAESNLDSANIVFVEVDEDTSTYDGDHIAGAIKLDWRADLQDPIKRDFIDTQQFSKLLGDRGISNDNTV
ILYGGNNNWFAAYAYWYFKLYRHDKVKLLDGGRKKWELDGRPLSTDTVTRPATSYAAAAPDNTIRAFRDEVIASIKIKNL
VDVRSPDEFSGKLLAPAHLPQEQSQRPGHIPSAINIPWSKAANEDGTFKSDEQLAKLYADAGLDRLKETIVYCRIGERSS
HTWFVLRELLGYQNVKNYDGSWTEYGSLVGVPIELGS

Sequences:

>Translated_277_residues
MARSDVLVSADWAESNLDSANIVFVEVDEDTSTYDGDHIAGAIKLDWRADLQDPIKRDFIDTQQFSKLLGDRGISNDNTV
ILYGGNNNWFAAYAYWYFKLYRHDKVKLLDGGRKKWELDGRPLSTDTVTRPATSYAAAAPDNTIRAFRDEVIASIKIKNL
VDVRSPDEFSGKLLAPAHLPQEQSQRPGHIPSAINIPWSKAANEDGTFKSDEQLAKLYADAGLDRLKETIVYCRIGERSS
HTWFVLRELLGYQNVKNYDGSWTEYGSLVGVPIELGS
>Mature_276_residues
ARSDVLVSADWAESNLDSANIVFVEVDEDTSTYDGDHIAGAIKLDWRADLQDPIKRDFIDTQQFSKLLGDRGISNDNTVI
LYGGNNNWFAAYAYWYFKLYRHDKVKLLDGGRKKWELDGRPLSTDTVTRPATSYAAAAPDNTIRAFRDEVIASIKIKNLV
DVRSPDEFSGKLLAPAHLPQEQSQRPGHIPSAINIPWSKAANEDGTFKSDEQLAKLYADAGLDRLKETIVYCRIGERSSH
TWFVLRELLGYQNVKNYDGSWTEYGSLVGVPIELGS

Specific function: May be a sulfotransferase involved in the formation of thiosulfate

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains

Homologues:

Organism=Homo sapiens, GI17402865, Length=243, Percent_Identity=28.8065843621399, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI194473668, Length=238, Percent_Identity=26.890756302521, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI61835204, Length=238, Percent_Identity=26.890756302521, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI194473681, Length=238, Percent_Identity=26.890756302521, Blast_Score=91, Evalue=8e-19,
Organism=Escherichia coli, GI87082121, Length=280, Percent_Identity=27.5, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87081967, Length=248, Percent_Identity=28.2258064516129, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17561888, Length=242, Percent_Identity=30.5785123966942, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71997283, Length=256, Percent_Identity=30.078125, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115534702, Length=211, Percent_Identity=30.3317535545024, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): THTR_MYCLE (Q50036)

Other databases:

- EMBL:   U15182
- EMBL:   AL583924
- PIR:   A87184
- RefSeq:   NP_302440.1
- ProteinModelPortal:   Q50036
- SMR:   Q50036
- EnsemblBacteria:   EBMYCT00000029233
- GeneID:   908836
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML2198
- NMPDR:   fig|272631.1.peg.1312
- Leproma:   ML2198
- GeneTree:   EBGT00050000015573
- HOGENOM:   HBG709927
- OMA:   PGVVFVE
- ProtClustDB:   CLSK2460599
- BioCyc:   MLEP272631:ML2198-MONOMER
- BRENDA:   2.8.1.1
- InterPro:   IPR001763
- InterPro:   IPR001307
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 31095; Mature: 30964

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00380 RHODANESE_1; PS00683 RHODANESE_2; PS50206 RHODANESE_3

Important sites: ACT_SITE 233-233 BINDING 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSDVLVSADWAESNLDSANIVFVEVDEDTSTYDGDHIAGAIKLDWRADLQDPIKRDFI
CCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHCC
DTQQFSKLLGDRGISNDNTVILYGGNNNWFAAYAYWYFKLYRHDKVKLLDGGRKKWELDG
CHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEHHEEEHHHHHCCEEEEECCCCEEEEECC
RPLSTDTVTRPATSYAAAAPDNTIRAFRDEVIASIKIKNLVDVRSPDEFSGKLLAPAHLP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEHHHEECCCCCCCCCCCEECCCCCC
QEQSQRPGHIPSAINIPWSKAANEDGTFKSDEQLAKLYADAGLDRLKETIVYCRIGERSS
HHHCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHEEEEEEECCCCC
HTWFVLRELLGYQNVKNYDGSWTEYGSLVGVPIELGS
CHHHHHHHHHCCCCCCCCCCCCHHCCCEEEEEEEECC
>Mature Secondary Structure 
ARSDVLVSADWAESNLDSANIVFVEVDEDTSTYDGDHIAGAIKLDWRADLQDPIKRDFI
CCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCHHHHHHHCC
DTQQFSKLLGDRGISNDNTVILYGGNNNWFAAYAYWYFKLYRHDKVKLLDGGRKKWELDG
CHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEHHEEEHHHHHCCEEEEECCCCEEEEECC
RPLSTDTVTRPATSYAAAAPDNTIRAFRDEVIASIKIKNLVDVRSPDEFSGKLLAPAHLP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEHHHEECCCCCCCCCCCEECCCCCC
QEQSQRPGHIPSAINIPWSKAANEDGTFKSDEQLAKLYADAGLDRLKETIVYCRIGERSS
HHHCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHEEEEEEECCCCC
HTWFVLRELLGYQNVKNYDGSWTEYGSLVGVPIELGS
CHHHHHHHHHCCCCCCCCCCCCHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002