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The map label for this gene is rmlB [H]

Identifier: 221230542

GI number: 221230542

Start: 2348258

End: 2349259

Strand: Direct

Name: rmlB [H]

Synonym: MLBr_01964

Alternate gene names: 221230542

Gene position: 2348258-2349259 (Clockwise)

Preceding gene: 221230533

Following gene: 221230543

Centisome position: 71.85

GC content: 59.08

Gene sequence:

>1002_bases
TTGACCATGCGGTTATTGGTCACCGGCGGAGCGGGCTTTATCGGAGCCAATTTTGTGCATAACGCGGTGCGCGACCATCC
CGACGATACGGTGACGGTGCTAGACGCCATGACTTACGCAGGCAGACACGAGTCGCTGGCCGATGTCGACCATGCTGTCA
GGCTGGTGCAAGGCAATATCACCGACACCAAGCTGGTTTTCCGACTGGTCGCCGAGTCCGACGTGGTTGTGCATTTCGCC
GCCGAAACCCATGTGGACAACGCGCTCGACGACCCAGAACCGTTCCTACGCACCAACGTCATCGGGACGTTCACCATCCT
GGAGGCAGTGCGACACCACAGTGTGCGGCTGCACCACATCTCGACCGACGAAGTCTATGGCGACCTAGAGCTCGACGAAC
CAACAAGGTTTACTGAGTCAACGCCCTACAACCCGTCGAGTCCGTACTCGGCAACCAAAGCCGCCGGCGACATGTTGGTC
CGGGCTTGGGTGCGCTCCTACGGCGTGCGTGCGACGATTTCCAACTGCTCCAACAACTACGGACCATACCAGCACGTCGA
AAAGTTCATTCCGCGCCAAATCACCAATGTGCTCACCGGCCGCCGGCCCAAGTTATACGGCACCGGCGTCAACGTCCGCG
ACTGGATCCACGTCGACGACCACAACAGCGCCGTCTGGCAAATCCTCGAGAAAGGCCAGATAGGCCGCACTTACCTCATC
GGAGCCGAAAACGGGCATAACAACCTGACCGTGTTGCGCACACTGCTACAGATGATGGGCCGAGACCCCAACGATTTCGA
CCATGTCACCGACCGCGTCGGCCACGACTTACGGTACGCGATCGACCCAACACCGCTGTACAACGAATTATGCTGGGCTC
CAAAGCACGCCAACTTCGACGAGGGATTGCGCGCCACCATTGACTGGTATCGGAACAACGAATCGTGGTGGAGACCGTTG
AAGGACGCCGTGGAAGCGCGCTACGAAGAACGAGGTCGGTGA

Upstream 100 bases:

>100_bases
AAGGAGGATCACCTGGCTTCAGCACTGGCCGAACTGACAGGGCCGCTCTGACTGATTCACCTGTGACCAGGCCCTTACCT
GGTTAACGTTCCGCTAGGGT

Downstream 100 bases:

>100_bases
AATGAACGTCAAAGAACTGGATATCTCGGGCGCCTGGGAGATCACCCCTACTATTCATAGCGATTCCCGCGGAATGTTTT
TCGAATGGCTCACAGCCAGT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTMRLLVTGGAGFIGANFVHNAVRDHPDDTVTVLDAMTYAGRHESLADVDHAVRLVQGNITDTKLVFRLVAESDVVVHFA
AETHVDNALDDPEPFLRTNVIGTFTILEAVRHHSVRLHHISTDEVYGDLELDEPTRFTESTPYNPSSPYSATKAAGDMLV
RAWVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGTGVNVRDWIHVDDHNSAVWQILEKGQIGRTYLI
GAENGHNNLTVLRTLLQMMGRDPNDFDHVTDRVGHDLRYAIDPTPLYNELCWAPKHANFDEGLRATIDWYRNNESWWRPL
KDAVEARYEERGR

Sequences:

>Translated_333_residues
MTMRLLVTGGAGFIGANFVHNAVRDHPDDTVTVLDAMTYAGRHESLADVDHAVRLVQGNITDTKLVFRLVAESDVVVHFA
AETHVDNALDDPEPFLRTNVIGTFTILEAVRHHSVRLHHISTDEVYGDLELDEPTRFTESTPYNPSSPYSATKAAGDMLV
RAWVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGTGVNVRDWIHVDDHNSAVWQILEKGQIGRTYLI
GAENGHNNLTVLRTLLQMMGRDPNDFDHVTDRVGHDLRYAIDPTPLYNELCWAPKHANFDEGLRATIDWYRNNESWWRPL
KDAVEARYEERGR
>Mature_332_residues
TMRLLVTGGAGFIGANFVHNAVRDHPDDTVTVLDAMTYAGRHESLADVDHAVRLVQGNITDTKLVFRLVAESDVVVHFAA
ETHVDNALDDPEPFLRTNVIGTFTILEAVRHHSVRLHHISTDEVYGDLELDEPTRFTESTPYNPSSPYSATKAAGDMLVR
AWVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGTGVNVRDWIHVDDHNSAVWQILEKGQIGRTYLIG
AENGHNNLTVLRTLLQMMGRDPNDFDHVTDRVGHDLRYAIDPTPLYNELCWAPKHANFDEGLRATIDWYRNNESWWRPLK
DAVEARYEERGR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=334, Percent_Identity=33.5329341317365, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI42516563, Length=313, Percent_Identity=24.2811501597444, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI4504031, Length=290, Percent_Identity=23.7931034482759, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=44.6376811594203, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI1788353, Length=362, Percent_Identity=42.5414364640884, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI1788366, Length=187, Percent_Identity=31.0160427807487, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=325, Percent_Identity=32.6153846153846, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI115532424, Length=318, Percent_Identity=31.7610062893082, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17539422, Length=192, Percent_Identity=29.6875, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI133901788, Length=192, Percent_Identity=29.6875, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI133901790, Length=192, Percent_Identity=29.6875, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI133901786, Length=192, Percent_Identity=29.6875, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=192, Percent_Identity=29.6875, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=185, Percent_Identity=29.1891891891892, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=335, Percent_Identity=22.9850746268657, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=327, Percent_Identity=26.2996941896024, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24158427, Length=299, Percent_Identity=24.4147157190635, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356223, Length=314, Percent_Identity=23.5668789808917, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 37799; Mature: 37667

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMRLLVTGGAGFIGANFVHNAVRDHPDDTVTVLDAMTYAGRHESLADVDHAVRLVQGNI
CEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
TDTKLVFRLVAESDVVVHFAAETHVDNALDDPEPFLRTNVIGTFTILEAVRHHSVRLHHI
CHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEE
STDEVYGDLELDEPTRFTESTPYNPSSPYSATKAAGDMLVRAWVRSYGVRATISNCSNNY
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHCCCCC
GPYQHVEKFIPRQITNVLTGRRPKLYGTGVNVRDWIHVDDHNSAVWQILEKGQIGRTYLI
CCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEE
GAENGHNNLTVLRTLLQMMGRDPNDFDHVTDRVGHDLRYAIDPTPLYNELCWAPKHANFD
ECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCC
EGLRATIDWYRNNESWWRPLKDAVEARYEERGR
CCHHEEEHEEECCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TMRLLVTGGAGFIGANFVHNAVRDHPDDTVTVLDAMTYAGRHESLADVDHAVRLVQGNI
EEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
TDTKLVFRLVAESDVVVHFAAETHVDNALDDPEPFLRTNVIGTFTILEAVRHHSVRLHHI
CHHHHHHHHHHCCCEEEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEE
STDEVYGDLELDEPTRFTESTPYNPSSPYSATKAAGDMLVRAWVRSYGVRATISNCSNNY
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHCCCCC
GPYQHVEKFIPRQITNVLTGRRPKLYGTGVNVRDWIHVDDHNSAVWQILEKGQIGRTYLI
CCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEE
GAENGHNNLTVLRTLLQMMGRDPNDFDHVTDRVGHDLRYAIDPTPLYNELCWAPKHANFD
ECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCCCCC
EGLRATIDWYRNNESWWRPLKDAVEARYEERGR
CCHHEEEHEEECCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]