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The map label for this gene is gloB [H]

Identifier: 221230504

GI number: 221230504

Start: 2295449

End: 2296165

Strand: Direct

Name: gloB [H]

Synonym: MLBr_01912

Alternate gene names: 221230504

Gene position: 2295449-2296165 (Clockwise)

Preceding gene: 221230494

Following gene: 221230505

Centisome position: 70.24

GC content: 58.58

Gene sequence:

>717_bases
GTGTCAGATTCGGACCGGCTGTACTTTCGGCAACTGCTCTCCGGACGCGATTTCGCCGTTGACGATGTTGTCGCAACGCA
GATGCGCAATTTTGCCTACCTGATCGGCGACCGCCAGACTGGGGACTGCGTCGTGGTCGACCCGGCGTACGCCGCTGGTG
ATCTGGTGGACGCACTCGAAGCGGACGGTATGCGGTTGTCTGGAGTGCTGGTGACTCACCATCACCCCGACCATGTGGGC
GGGGTGATGATGGGCTTTCAGCTAAAGGGATTGACCGAGCTGCTCGAGCGAGTAACTGTGCCCGTGCACGTTAATACCCA
TGAGGCGCTATGGATTTCACGAGTCACAGGAATTGACATCGGTCATCTGACCACTCATGAGCACCGCGACAAGCTCAGCG
TTGGCGATATCGAAATTGAGCTGCTGCACACACCGGGTCATACGCCGGGCAGCCAGTGTTTTCTGCTCGATGGCCTGCTG
GTCGCCGGAGACACGTTGTTTCTGGAGGGTTGCGGCCGTACCGACTTTCCGGGTGGCAATTCCGACGAGATGTACCGCAG
CCTGCAGCAGCTCGCGCAACTTCCGGGTAACCCTACAGTGTTCCCAGGGCACTGGTATTCAACCGCTCCCAGTGCCACGC
TATCGATGGTCAAGTGCTCCAACTACGTGTACCGTGCCGCGGATCTCAATCAGTGGCGAATGTTGATGGACAGCTGA

Upstream 100 bases:

>100_bases
CACTTAATATACGCCACCAATCGGTAGCCTGCCGACAATTTATGCAATGGTGCTCTAGACAGTCTTGGACGAGTATTTAG
ACGAGACCTAGTCTAGTGAG

Downstream 100 bases:

>100_bases
GGAATTGCTGTGCAATGCACTGACCTACCAGAACTACGGACCACAAGGGAAATGCGTCCGACATATTACGCGCCGGGATC
GAGATCCAGCCCCTTACAGC

Product: putative glyoxylase II

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSDSDRLYFRQLLSGRDFAVDDVVATQMRNFAYLIGDRQTGDCVVVDPAYAAGDLVDALEADGMRLSGVLVTHHHPDHVG
GVMMGFQLKGLTELLERVTVPVHVNTHEALWISRVTGIDIGHLTTHEHRDKLSVGDIEIELLHTPGHTPGSQCFLLDGLL
VAGDTLFLEGCGRTDFPGGNSDEMYRSLQQLAQLPGNPTVFPGHWYSTAPSATLSMVKCSNYVYRAADLNQWRMLMDS

Sequences:

>Translated_238_residues
MSDSDRLYFRQLLSGRDFAVDDVVATQMRNFAYLIGDRQTGDCVVVDPAYAAGDLVDALEADGMRLSGVLVTHHHPDHVG
GVMMGFQLKGLTELLERVTVPVHVNTHEALWISRVTGIDIGHLTTHEHRDKLSVGDIEIELLHTPGHTPGSQCFLLDGLL
VAGDTLFLEGCGRTDFPGGNSDEMYRSLQQLAQLPGNPTVFPGHWYSTAPSATLSMVKCSNYVYRAADLNQWRMLMDS
>Mature_237_residues
SDSDRLYFRQLLSGRDFAVDDVVATQMRNFAYLIGDRQTGDCVVVDPAYAAGDLVDALEADGMRLSGVLVTHHHPDHVGG
VMMGFQLKGLTELLERVTVPVHVNTHEALWISRVTGIDIGHLTTHEHRDKLSVGDIEIELLHTPGHTPGSQCFLLDGLLV
AGDTLFLEGCGRTDFPGGNSDEMYRSLQQLAQLPGNPTVFPGHWYSTAPSATLSMVKCSNYVYRAADLNQWRMLMDS

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=197, Percent_Identity=31.4720812182741, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI94538322, Length=197, Percent_Identity=31.4720812182741, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI41327741, Length=243, Percent_Identity=29.6296296296296, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI116642887, Length=187, Percent_Identity=35.2941176470588, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI21703352, Length=187, Percent_Identity=35.2941176470588, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1787158, Length=213, Percent_Identity=30.5164319248826, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786406, Length=178, Percent_Identity=31.4606741573034, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536925, Length=213, Percent_Identity=28.6384976525822, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356335, Length=189, Percent_Identity=33.8624338624339, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24667703, Length=189, Percent_Identity=33.8624338624339, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24667711, Length=207, Percent_Identity=32.3671497584541, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221330176, Length=209, Percent_Identity=28.2296650717703, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001018
- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 26196; Mature: 26065

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSDRLYFRQLLSGRDFAVDDVVATQMRNFAYLIGDRQTGDCVVVDPAYAAGDLVDALE
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEECCCCCCEEEECCCCHHHHHHHHHH
ADGMRLSGVLVTHHHPDHVGGVMMGFQLKGLTELLERVTVPVHVNTHEALWISRVTGIDI
CCCEEEEEEEEECCCCCHHCCEEEEEEHHHHHHHHHHCEEEEEECCCCEEEEEEECCCEE
GHLTTHEHRDKLSVGDIEIELLHTPGHTPGSQCFLLDGLLVAGDTLFLEGCGRTDFPGGN
CCCCCCCCCCCCEECCEEEEEEECCCCCCCCCEEEECCHHHHCCEEEEECCCCCCCCCCC
SDEMYRSLQQLAQLPGNPTVFPGHWYSTAPSATLSMVKCSNYVYRAADLNQWRMLMDS
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHEECCCEEEEECCCCHHHHCCCC
>Mature Secondary Structure 
SDSDRLYFRQLLSGRDFAVDDVVATQMRNFAYLIGDRQTGDCVVVDPAYAAGDLVDALE
CCCHHHHHHHHHCCCCCCHHHHHHHHHHCEEEEEECCCCCCEEEECCCCHHHHHHHHHH
ADGMRLSGVLVTHHHPDHVGGVMMGFQLKGLTELLERVTVPVHVNTHEALWISRVTGIDI
CCCEEEEEEEEECCCCCHHCCEEEEEEHHHHHHHHHHCEEEEEECCCCEEEEEEECCCEE
GHLTTHEHRDKLSVGDIEIELLHTPGHTPGSQCFLLDGLLVAGDTLFLEGCGRTDFPGGN
CCCCCCCCCCCCEECCEEEEEEECCCCCCCCCEEEECCHHHHCCEEEEECCCCCCCCCCC
SDEMYRSLQQLAQLPGNPTVFPGHWYSTAPSATLSMVKCSNYVYRAADLNQWRMLMDS
HHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHEECCCEEEEECCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA