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The map label for this gene is nfo

Identifier: 221230485

GI number: 221230485

Start: 2268112

End: 2268870

Strand: Reverse

Name: nfo

Synonym: MLBr_01889

Alternate gene names: 221230485

Gene position: 2268870-2268112 (Counterclockwise)

Preceding gene: 221230486

Following gene: 221230484

Centisome position: 69.43

GC content: 59.16

Gene sequence:

>759_bases
GTGCTCATCGGTTCGCATGTCAGCTCCACCGATCCGCTCGCCGCAGCGGAGGTTGAAGGCGCTGACGTAGTGCAGATTTT
CCTCGGTAACCCGCAGAGCTGGAAGGCGCCCACACTGCGTAGCGATGCCGATGTTCTGAAGGCTACGGCTTTGCCGGTGT
ACGTTCATGCGCCCTACTTGATCAACGTCGCGTCGGCTAACAGCCGCGTGCGGATCCCATCACGCAAGATCCTGCAGCAA
ACTTGTGACGCAGCCGCCGACATCGGCGCTGCTGCCGTGGTCGTGCATGGTGGGTACGTCGCCGATGACAATGACCTCGA
GGATGGTTTCCAACGCTGGCGTAAAGCACTCGACCAACTGCAAACTGACGTTCCGGTCTACCTCGAAAACACGGCCGGCG
GCGATCACGCGATGGCTCGCCGCTTTGATACCATCGCCCGACTCTGGGATGTTATTGGTGAAACCGGTATCGGTTTTTGC
CTGGACACCTGTCATGCTTGGGCGGCTGGCGAGGGGCTGATTCATGTTGTGGATCGCATCAAGGCCATCACTGGTCGGAT
CGACCTGGTGCACTGCAATGATTCCAAGGACGAAGCGGGTTCTGGTCGTGACCGTCACGCCAACTTGGGTAGTGGTCAAA
TCGATGCCGAGTTGCTGGTAGCAGCAGTCAAGGTAGCGGGTGCGCCGGTGATCTGCGAAACCGCCGAGGAAGGTCGCAAA
GACGACATTGCGTTCTTGCGCGAAAAGACCAGCGGCTGA

Upstream 100 bases:

>100_bases
GTGGGCGCTGGTGCTTGTTGTGCCCCCAAAGAGTTAGACACCCATGGCCACATTGTGACGGTAGCGCTGGGCGTAGGCAT
CTTTCGCTAAACTGGGCGGT

Downstream 100 bases:

>100_bases
CGCAGCAGCGGGATCGCTCCGTCTACTCGAGGGCGTTCATGGTCCCCTTCCCCAATGCCTCCGCGTTAGTGTGACGTGTA
TTATGCGATTGTCGGAGAAA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLIGSHVSSTDPLAAAEVEGADVVQIFLGNPQSWKAPTLRSDADVLKATALPVYVHAPYLINVASANSRVRIPSRKILQQ
TCDAAADIGAAAVVVHGGYVADDNDLEDGFQRWRKALDQLQTDVPVYLENTAGGDHAMARRFDTIARLWDVIGETGIGFC
LDTCHAWAAGEGLIHVVDRIKAITGRIDLVHCNDSKDEAGSGRDRHANLGSGQIDAELLVAAVKVAGAPVICETAEEGRK
DDIAFLREKTSG

Sequences:

>Translated_252_residues
MLIGSHVSSTDPLAAAEVEGADVVQIFLGNPQSWKAPTLRSDADVLKATALPVYVHAPYLINVASANSRVRIPSRKILQQ
TCDAAADIGAAAVVVHGGYVADDNDLEDGFQRWRKALDQLQTDVPVYLENTAGGDHAMARRFDTIARLWDVIGETGIGFC
LDTCHAWAAGEGLIHVVDRIKAITGRIDLVHCNDSKDEAGSGRDRHANLGSGQIDAELLVAAVKVAGAPVICETAEEGRK
DDIAFLREKTSG
>Mature_252_residues
MLIGSHVSSTDPLAAAEVEGADVVQIFLGNPQSWKAPTLRSDADVLKATALPVYVHAPYLINVASANSRVRIPSRKILQQ
TCDAAADIGAAAVVVHGGYVADDNDLEDGFQRWRKALDQLQTDVPVYLENTAGGDHAMARRFDTIARLWDVIGETGIGFC
LDTCHAWAAGEGLIHVVDRIKAITGRIDLVHCNDSKDEAGSGRDRHANLGSGQIDAELLVAAVKVAGAPVICETAEEGRK
DDIAFLREKTSG

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=260, Percent_Identity=30, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17531193, Length=262, Percent_Identity=24.8091603053435, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322735, Length=278, Percent_Identity=25.5395683453237, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_MYCLB (B8ZSC5)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503901.1
- ProteinModelPortal:   B8ZSC5
- SMR:   B8ZSC5
- EnsemblBacteria:   EBMYCT00000086940
- GeneID:   7325283
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017851
- HOGENOM:   HBG565018
- OMA:   NRIRIPS
- ProtClustDB:   PRK01060
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 26861; Mature: 26861

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGSHVSSTDPLAAAEVEGADVVQIFLGNPQSWKAPTLRSDADVLKATALPVYVHAPYL
CEECCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCEEEEECCEE
INVASANSRVRIPSRKILQQTCDAAADIGAAAVVVHGGYVADDNDLEDGFQRWRKALDQL
EEEECCCCEEECCHHHHHHHHHHHHHHCCCEEEEEECCEECCCCCHHHHHHHHHHHHHHH
QTDVPVYLENTAGGDHAMARRFDTIARLWDVIGETGIGFCLDTCHAWAAGEGLIHVVDRI
HCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
KAITGRIDLVHCNDSKDEAGSGRDRHANLGSGQIDAELLVAAVKVAGAPVICETAEEGRK
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECHHCCCH
DDIAFLREKTSG
HHHHHHHHCCCC
>Mature Secondary Structure
MLIGSHVSSTDPLAAAEVEGADVVQIFLGNPQSWKAPTLRSDADVLKATALPVYVHAPYL
CEECCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCEEEEECCEE
INVASANSRVRIPSRKILQQTCDAAADIGAAAVVVHGGYVADDNDLEDGFQRWRKALDQL
EEEECCCCEEECCHHHHHHHHHHHHHHCCCEEEEEECCEECCCCCHHHHHHHHHHHHHHH
QTDVPVYLENTAGGDHAMARRFDTIARLWDVIGETGIGFCLDTCHAWAAGEGLIHVVDRI
HCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
KAITGRIDLVHCNDSKDEAGSGRDRHANLGSGQIDAELLVAAVKVAGAPVICETAEEGRK
HHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECHHCCCH
DDIAFLREKTSG
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA