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The map label for this gene is tuf [H]

Identifier: 221230481

GI number: 221230481

Start: 2255715

End: 2256905

Strand: Reverse

Name: tuf [H]

Synonym: MLBr_01877

Alternate gene names: 221230481

Gene position: 2256905-2255715 (Counterclockwise)

Preceding gene: 221230482

Following gene: 221230480

Centisome position: 69.06

GC content: 59.28

Gene sequence:

>1191_bases
GTGGCGAAGGCGAAGTTCGAGCGGACGAAGCCGCACGTCAACATCGGGACCATCGGTCACGTTGACCACGGCAAGACCAC
ATTGACCGCGGCTATTACCAAGGTCCTGCATGACAAGTTCCCCAACCTGAATGAGTCGCGCGCCTTTGACCAGATTGACA
ACGCGCCCGAGGAGCGTCAGCGCGGTATCACCATCAACATTTCCCACGTGGAGTATCAGACCGAGAAGCGTCACTATGCT
CACGTCGACGCCCCGGGGCACGCCGACTACATTAAGAACATGATCACCGGTGCGGCCCAGATGGATGGTGCGATTCTGGT
GGTCGCTGCTACAGACGGCCCGATGCCGCAGACTCGCGAGCACGTGCTGCTCGCTCGTCAGGTGGGTGTACCTTACATCC
TGGTCGCACTTAACAAGTCCGACGCCGTGGACGACGAGGAACTACTCGAGCTTGTCGAGATGGAAGTCCGTGAGTTGCTG
GCTGCCCAGGAATTCGACGAGGACGCCCCGGTTGTGCGTGTCTCGGCATTGAAGGCGCTCGAGGGTGACGCCAAGTGGGT
CGAGTCTGTCACACAGTTGATGGACGCTGTCGACGAGTCGATCCCGGCTCCAGTCCGCGAGACTGACAAGCCGTTCCTGA
TGCCCGTTGAGGACGTCTTCACTATCACCGGTCGTGGCACCGTGGTCACCGGTCGGGTGGAGCGCGGCGTGGTCAACGTG
AACGAGGAAGTTGAGATCGTCGGCATTCGTCAGACGACCACCAAGACCACCGTCACGGGTGTGGAAATGTTTCGTAAGCT
GCTCGACCAAGGTCAGGCCGGTGATAACGTTGGTCTGTTGTTGCGTGGCATCAAGCGCGAGGACGTCGAGCGTGGTCAGG
TGGTCATCAAACCCGGCACCACCACCCCGCATACCGAGTTCGAAGGCCAAGTATACATCCTGTCCAAGGACGAAGGTGGT
CGGCACACACCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGTACCACTGATGTGACCGGTGTGGTGACTTTGCC
GGAGGGCACCGAGATGGTGATGCCAGGTGACAACACCAACATCTCGGTGACGTTGATTCAGCCCGTTGCCATGGACGAGG
GTTTGCGCTTCGCGATCCGCGAAGGTGGTCGCACTGTTGGTGCCGGCCGGGTCGTCAAGATCATCAAGTAA

Upstream 100 bases:

>100_bases
TGGTCGGAAAAGCCTACGAGTTATGCTGACGCGGTCACTACCGCGGATAAATTACTAACATTGCTTTTTACAAGCACCAA
TAGTCCAGGAGGACACAGAA

Downstream 100 bases:

>100_bases
GTCGCATCAACGGGCCAGGTGGTTACAACGGTCCACATTTGCGAAACGGCGCTCATCCGAAAGGATAGGCGCCGTTTCTT
TTGAGTTTATGCGGCTCGTG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPNLNESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKSDAVDDEELLELVEMEVRELL
AAQEFDEDAPVVRVSALKALEGDAKWVESVTQLMDAVDESIPAPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVVNV
NEEVEIVGIRQTTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVIKPGTTTPHTEFEGQVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVTLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPNLNESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKSDAVDDEELLELVEMEVRELL
AAQEFDEDAPVVRVSALKALEGDAKWVESVTQLMDAVDESIPAPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVVNV
NEEVEIVGIRQTTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVIKPGTTTPHTEFEGQVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVTLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPNLNESRAFDQIDNAPEERQRGITINISHVEYQTEKRHYAH
VDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKSDAVDDEELLELVEMEVRELLA
AQEFDEDAPVVRVSALKALEGDAKWVESVTQLMDAVDESIPAPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVVNVN
EEVEIVGIRQTTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVIKPGTTTPHTEFEGQVYILSKDEGGR
HTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVTLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=401, Percent_Identity=54.8628428927681, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=31.25, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI4503475, Length=358, Percent_Identity=33.5195530726257, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=29.8876404494382, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=29.8876404494382, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI194018522, Length=446, Percent_Identity=24.8878923766816, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI194097354, Length=446, Percent_Identity=24.8878923766816, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI194018520, Length=446, Percent_Identity=24.8878923766816, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI46094014, Length=447, Percent_Identity=25.2796420581655, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI54607086, Length=325, Percent_Identity=27.0769230769231, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI4503507, Length=307, Percent_Identity=26.0586319218241, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI157426893, Length=289, Percent_Identity=26.643598615917, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=75, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=75, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=387, Percent_Identity=30.2325581395349, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI48994988, Length=278, Percent_Identity=33.4532374100719, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1789108, Length=329, Percent_Identity=28.2674772036474, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788922, Length=331, Percent_Identity=25.6797583081571, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=396, Percent_Identity=49.7474747474747, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25141371, Length=374, Percent_Identity=44.6524064171123, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17552884, Length=446, Percent_Identity=30.0448430493274, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=446, Percent_Identity=30.0448430493274, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=28.735632183908, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=28.7037037037037, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=28.5057471264368, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=31.3868613138686, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=24.7787610619469, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=150, Percent_Identity=42, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=296, Percent_Identity=26.6891891891892, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=26.7241379310345, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17565438, Length=423, Percent_Identity=22.6950354609929, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=401, Percent_Identity=62.5935162094763, Blast_Score=491, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6325337, Length=328, Percent_Identity=34.7560975609756, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=328, Percent_Identity=34.7560975609756, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=25.6410256410256, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=350, Percent_Identity=28.8571428571429, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320863, Length=285, Percent_Identity=25.9649122807018, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=59.0909090909091, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=59.0909090909091, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=50.1285347043702, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=439, Percent_Identity=30.5239179954442, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45553816, Length=439, Percent_Identity=30.5239179954442, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24651721, Length=439, Percent_Identity=30.5239179954442, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17864154, Length=439, Percent_Identity=30.5239179954442, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=29.8206278026906, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=29.8206278026906, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45550900, Length=340, Percent_Identity=31.4705882352941, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=30.4918032786885, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=29.2899408284024, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=26.4285714285714, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=26.4285714285714, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78706572, Length=257, Percent_Identity=26.4591439688716, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28572034, Length=187, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43668; Mature: 43537

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPNLNESRAFDQIDNAPEERQ
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKSDAVDDEELLELVEMEVRELLAAQEFDEDAPVVRVSALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
EGDAKWVESVTQLMDAVDESIPAPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVVNV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEEEECCCCEEEC
NEEVEIVGIRQTTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVIKPGT
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCEEEEECCC
TTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTN
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
ISVTLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK
EEEEEEEEEECCCCCEEEECCCCCEECCCCEEEEEC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPNLNESRAFDQIDNAPEERQ
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKSDAVDDEELLELVEMEVRELLAAQEFDEDAPVVRVSALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHH
EGDAKWVESVTQLMDAVDESIPAPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVVNV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEEEECCCCEEEC
NEEVEIVGIRQTTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGIKREDVERGQVVIKPGT
CCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCCCCCEEEEECCC
TTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTN
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
ISVTLIQPVAMDEGLRFAIREGGRTVGAGRVVKIIK
EEEEEEEEEECCCCCEEEECCCCCEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA