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The map label for this gene is adhE2 [C]

Identifier: 221230427

GI number: 221230427

Start: 2160202

End: 2161287

Strand: Direct

Name: adhE2 [C]

Synonym: MLBr_01784

Alternate gene names: 221230427

Gene position: 2160202-2161287 (Clockwise)

Preceding gene: 221230423

Following gene: 221230430

Centisome position: 66.1

GC content: 62.15

Gene sequence:

>1086_bases
ATGAGTCAAACAGTACGCGGAGTAATCTCGCGCAAGAAGGACGAACCCGTGGAACTGGTGGACATCGTCATCCCAGATCC
CGGGCCGGGGGAGGCCGTGGTCGACGTCACCGCGTGCGGAGTGTGCCACACTGACCTGACCTACCGCGAAGGCGGCATCA
ATGACAGGTATCCGTTCCTGCTCGGCCACGAAGCAGCCGGCACGGTCGAGGTCGTCGGGCCGGGCGTGACCGCGGTCGAA
CCGGGCGACTTCGTAATCCTGAACTGGCGAGCCGTGTGCGGGCAGTGCCGGGCCTGCAAACGCGGCAGGCCAAGCTACTG
TTTCGACACATTCAACGCCCAACAGAAAATGACACTGATCGACGGCACCGAACTTACCCCGGCGCTGGGCATCGGAGCCT
TCGCCGACAAGACGCTAGTGCACTCTGGTCAATGCACCAAAGTCGATCCCGCTGCCGATCCAGCGGTCGCCGGCCTGCTG
GGCTGCGGGGTGATGGCCGGGTTAGGCGCCGCAATCAATACCGCCGCAGTGAGCCGCGACGACACCGTCGCGGTGATCGG
CTGTGGCGGTGTGGGTGATGCCGCAATCTCCGGGGCCGCGCTGGTCGGTGCCAACCGGATCATCGCGGTCGACATCGATG
ACACGAAACTGGAGTGGGCACGCACGTTCGGTGCCACCCACACCGTTAACGCCCTTGAACTCGAAGTCGTCAAAACCATC
CAGGATCTCACCAGTGGCTTCGGCGTCGACGTGGTAATCGACACGGTCGGCCGACCCGAAACCTGGAAGCAGGCCTTCTA
CGCCCGCGATCTCGCCGGAACCGTTGTACTGGTGGGTGTACCGACACCCGACATGCGCCTGGACATGCCGCTGCTAGACT
TCTTCTCTCATGGCGGCTCGCTTAAGTCATCGTGGTACGGTGATTGCCTACCTGAACGCGATTTCCCCACCCTTGTTGAC
CTCTATCTGCAGGGACGCCTTCCATTGGGCAAGTTCGTCTCCGAGCGCATTGGGCTGGGCGACGTCGAAGAAGCATTCCA
CAAGATACACGGTGGTAAAGTGCTGCGCTCGGTGGTTATGCTGTGA

Upstream 100 bases:

>100_bases
CATGAGCGTCGGACATCCTCTCGATGAATGCTTCGGTGGTTGCGAAGCCGGTCTCGGGCGCCGTCATACTAAGCAACGCT
ACAACCGTAGGTTGGACACC

Downstream 100 bases:

>100_bases
TTGAATGGGTAGTCCCCCAAAGAACCTTCGAACTCGACAGTAGCAGTTAGGAAATCGATAACAACATCTGGATAGTCGGT
GACGACACCGAGGTCGTGAT

Product: putative alcohol dehydrogenase (Zn dependent)

Products: NA

Alternate protein names: NAD/mycothiol-dependent formaldehyde dehydrogenase; MD-FALDH [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSQTVRGVISRKKDEPVELVDIVIPDPGPGEAVVDVTACGVCHTDLTYREGGINDRYPFLLGHEAAGTVEVVGPGVTAVE
PGDFVILNWRAVCGQCRACKRGRPSYCFDTFNAQQKMTLIDGTELTPALGIGAFADKTLVHSGQCTKVDPAADPAVAGLL
GCGVMAGLGAAINTAAVSRDDTVAVIGCGGVGDAAISGAALVGANRIIAVDIDDTKLEWARTFGATHTVNALELEVVKTI
QDLTSGFGVDVVIDTVGRPETWKQAFYARDLAGTVVLVGVPTPDMRLDMPLLDFFSHGGSLKSSWYGDCLPERDFPTLVD
LYLQGRLPLGKFVSERIGLGDVEEAFHKIHGGKVLRSVVML

Sequences:

>Translated_361_residues
MSQTVRGVISRKKDEPVELVDIVIPDPGPGEAVVDVTACGVCHTDLTYREGGINDRYPFLLGHEAAGTVEVVGPGVTAVE
PGDFVILNWRAVCGQCRACKRGRPSYCFDTFNAQQKMTLIDGTELTPALGIGAFADKTLVHSGQCTKVDPAADPAVAGLL
GCGVMAGLGAAINTAAVSRDDTVAVIGCGGVGDAAISGAALVGANRIIAVDIDDTKLEWARTFGATHTVNALELEVVKTI
QDLTSGFGVDVVIDTVGRPETWKQAFYARDLAGTVVLVGVPTPDMRLDMPLLDFFSHGGSLKSSWYGDCLPERDFPTLVD
LYLQGRLPLGKFVSERIGLGDVEEAFHKIHGGKVLRSVVML
>Mature_360_residues
SQTVRGVISRKKDEPVELVDIVIPDPGPGEAVVDVTACGVCHTDLTYREGGINDRYPFLLGHEAAGTVEVVGPGVTAVEP
GDFVILNWRAVCGQCRACKRGRPSYCFDTFNAQQKMTLIDGTELTPALGIGAFADKTLVHSGQCTKVDPAADPAVAGLLG
CGVMAGLGAAINTAAVSRDDTVAVIGCGGVGDAAISGAALVGANRIIAVDIDDTKLEWARTFGATHTVNALELEVVKTIQ
DLTSGFGVDVVIDTVGRPETWKQAFYARDLAGTVVLVGVPTPDMRLDMPLLDFFSHGGSLKSSWYGDCLPERDFPTLVDL
YLQGRLPLGKFVSERIGLGDVEEAFHKIHGGKVLRSVVML

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI34577061, Length=359, Percent_Identity=33.7047353760446, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI4501929, Length=359, Percent_Identity=33.7047353760446, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI4501933, Length=360, Percent_Identity=31.3888888888889, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=30.7065217391304, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI71565154, Length=369, Percent_Identity=31.1653116531165, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4501939, Length=360, Percent_Identity=30.8333333333333, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI262073058, Length=361, Percent_Identity=29.6398891966759, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI71565152, Length=372, Percent_Identity=30.6451612903226, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI71743840, Length=361, Percent_Identity=29.6398891966759, Blast_Score=158, Evalue=6e-39,
Organism=Escherichia coli, GI1786552, Length=367, Percent_Identity=33.2425068119891, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87081918, Length=281, Percent_Identity=30.2491103202847, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790045, Length=356, Percent_Identity=23.5955056179775, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788073, Length=342, Percent_Identity=26.3157894736842, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788407, Length=341, Percent_Identity=25.8064516129032, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787863, Length=341, Percent_Identity=23.7536656891496, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082125, Length=357, Percent_Identity=25.4901960784314, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786518, Length=285, Percent_Identity=27.0175438596491, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786825, Length=251, Percent_Identity=29.0836653386454, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788075, Length=334, Percent_Identity=25.4491017964072, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI226510992, Length=362, Percent_Identity=23.7569060773481, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790718, Length=336, Percent_Identity=23.5119047619048, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=382, Percent_Identity=31.9371727748691, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71997431, Length=367, Percent_Identity=31.3351498637602, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17565904, Length=332, Percent_Identity=33.1325301204819, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71988145, Length=365, Percent_Identity=26.3013698630137, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562582, Length=362, Percent_Identity=25.414364640884, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562584, Length=366, Percent_Identity=27.0491803278689, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320033, Length=361, Percent_Identity=32.9639889196676, Blast_Score=182, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6319621, Length=368, Percent_Identity=28.2608695652174, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=366, Percent_Identity=27.0491803278689, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=360, Percent_Identity=24.7222222222222, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322619, Length=349, Percent_Identity=27.2206303724928, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324486, Length=364, Percent_Identity=25.5494505494505, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319955, Length=349, Percent_Identity=27.2206303724928, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=301, Percent_Identity=24.5847176079734, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737895, Length=370, Percent_Identity=33.2432432432432, Blast_Score=174, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR017816
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.306 [H]

Molecular weight: Translated: 38110; Mature: 37979

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTVRGVISRKKDEPVELVDIVIPDPGPGEAVVDVTACGVCHTDLTYREGGINDRYPFL
CCHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEEECCEEECCCEECCCCCCCCCCEE
LGHEAAGTVEVVGPGVTAVEPGDFVILNWRAVCGQCRACKRGRPSYCFDTFNAQQKMTLI
EECCCCCEEEEECCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEE
DGTELTPALGIGAFADKTLVHSGQCTKVDPAADPAVAGLLGCGVMAGLGAAINTAAVSRD
CCCCCCHHHCCCCHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHEECCC
DTVAVIGCGGVGDAAISGAALVGANRIIAVDIDDTKLEWARTFGATHTVNALELEVVKTI
CCEEEEECCCCCCHHHCCEEEECCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHH
QDLTSGFGVDVVIDTVGRPETWKQAFYARDLAGTVVLVGVPTPDMRLDMPLLDFFSHGGS
HHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCHHHHHHHCCCC
LKSSWYGDCLPERDFPTLVDLYLQGRLPLGKFVSERIGLGDVEEAFHKIHGGKVLRSVVM
CCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHC
L
C
>Mature Secondary Structure 
SQTVRGVISRKKDEPVELVDIVIPDPGPGEAVVDVTACGVCHTDLTYREGGINDRYPFL
CHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEEECCEEECCCEECCCCCCCCCCEE
LGHEAAGTVEVVGPGVTAVEPGDFVILNWRAVCGQCRACKRGRPSYCFDTFNAQQKMTLI
EECCCCCEEEEECCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEE
DGTELTPALGIGAFADKTLVHSGQCTKVDPAADPAVAGLLGCGVMAGLGAAINTAAVSRD
CCCCCCHHHCCCCHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHEECCC
DTVAVIGCGGVGDAAISGAALVGANRIIAVDIDDTKLEWARTFGATHTVNALELEVVKTI
CCEEEEECCCCCCHHHCCEEEECCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHH
QDLTSGFGVDVVIDTVGRPETWKQAFYARDLAGTVVLVGVPTPDMRLDMPLLDFFSHGGS
HHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCHHHHHHHCCCC
LKSSWYGDCLPERDFPTLVDLYLQGRLPLGKFVSERIGLGDVEEAFHKIHGGKVLRSVVM
CCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9346279; 1597190; 9202149 [H]