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The map label for this gene is ilvB

Identifier: 221230380

GI number: 221230380

Start: 2044262

End: 2046139

Strand: Reverse

Name: ilvB

Synonym: MLBr_01696

Alternate gene names: 221230380

Gene position: 2046139-2044262 (Counterclockwise)

Preceding gene: 221230381

Following gene: 221230379

Centisome position: 62.61

GC content: 60.92

Gene sequence:

>1878_bases
GTGAGCGCACCGACCAAGCCACACGCCCGCCCGCAGGGGGCGGGTAACAGCGTGCCAAACACCGTGAAACCCGCCACGCA
ATTTCCCTCAAAGCCAGCTGTCGCAAAACTAGAACGCGTTTCACCTGAGCAACTTACCGGTGCTCAATCGGTGATTCGGT
CGTTGGAAGAACTCGATGTCGAGGTCATCTTCGGAATTCCCGGCGGTGCCGTCCTTCTGGTATATGACCCGCTGTTCTAC
TCAAAAAAGTTACGTCATGTCCTGGTCCGCCACGAGCAGGGCGCCGGCCACGCGGCCAGCGGCTACGCACACGTGACCGG
CAAGGTGGGGGTGTGCATGGCGACTTCTGGTCCTGGAGCCACCAATCTGGTGACACCGTTGGCCGATGCGCAGATGGACT
CAGTGCCGGTGGTCGCCATTACTGGGCAGGTCGGGCGCAGTCTGATCGGGACGGATGCCTTCCAAGAGGCCGACATCTCC
GGCATCACAATGCCGATAACCAAGCATAACTTTCTGGTTCGTGCCGGCGACGATATTCCCCGGGTACTGGCTGAGGCCTT
CCACATCGCGTCGTCGGGACGGCCGGGTGCGGTGCTCGTCGATATCCCGAAGGATGTGCTGCAGGGCCAGTGCAAGTTCA
GCTGGCCACCGAAAATGGACTTGCCCGGGTACAAGCCGAACACCAAACCGCACAACCGGCAGATCCGTGCGGCAGCCAAG
CTGATCGCTGACGCCCGCAAGCCTGTGCTGTATGTCGGCGGGGGCGTTATCCGCGGCGAAGCGACCGAGCAGCTACGGGA
TTTGGCCGAACTGACCGGCATCCCGGTGGTTAGCACGTTGATGGCACGTGGCGCGTTCCCCGACAGCCATCACCAGAACT
TGGGCATGCCCGGCATGCACGGCACCGTTGCGGCGGTGGCAGCGCTGCAGCGCAGCGATTTACTGATCGCACTGGGCACT
CGCTTTGACGACCGGGTGACTGGCAAGCTCGACTCCTTCGCGCCCGACGCCAAGGTTATACATGCCGACATCGACCCGGC
GGAGATCGGCAAGAACCGGCACGCTGACGTGCCGATTGTGGGCGACGTCAAAGCTGTTATTGTAGAGCTCATCGCGATGC
TGCGCCATTACGAGGTCCCCGGCAACATCGAAATGACCGACTGGTGGTCATATTTGGACGGTGTTCGCAAGACGTATCCG
TTGAGCTACAGTCCACAAAGCGACGGCACTCTGAGCCCGGAGTACGTTATCGAAAAGCTTGGTGAAATTGTTGGCCCGGA
GGCCGTATATGTCGCGGGTGTAGGTCAGCATCAGATGTGGGCGGCGCAGTTCATCTCCTACGAGAAGCCGCGTACTTGGC
TCAACTCAGGCGGTCTGGGCACCATGGGGTTTGCTATTCCGGCGGCCATGGGCGCCAAGATTGCTCGCCCGGAGGCAGAA
GTCTGGGCGATCGACGGCGATGGCTGCTTCCAGATGACCAACCAGGAGTTGGCTACGTGCGCTATCGAGGGCGCGCCGAT
CAAGGTGGCGTTGATCAACAACGGCAACCTGGGCATGGTGCGGCAATGGCAAGCCTTGTTTTATCAGGAGCGTTACTCGC
AGACCGACCTGGCCACCCACTCACACCGCATTCCGGACTTCGTGAAGTTGGCCGAGGCGTTGGGTTGCGTCGGATTGCGT
TGCGAGTGTGAAGAGGACGTTGTCGATGTGATCAATCAGGCCAGGGCGATCAACAACCGCCCTGTGGTGATCGACTTCAT
CGTCGGCGCCGATGCGCAGGTGTGGCCGATGGTCGCCGCTGGGGCCAGCAACGACGAGATCCAAGCTGCCCGCGGTATCC
GCCCGCTCTTTGATGACGAAAGCGAAGGGCATGTTTGA

Upstream 100 bases:

>100_bases
GCCAACGCGCAACCCTCGTGCAGCTAGCTCAGCTGTCGAGGGTTTTTTGTTGCCCGTCAACGTGACTGTCGAAGCGAATA
AGGACTAAAGAGGACACACA

Downstream 100 bases:

>100_bases
CTGTGCGTCGGCGACGATGCAGAGCCTAGCGATGAGGAGGAATGGCGCATAATGGTAGGGTTGCCAACGCACACCTTGTC
GGTTCTGGTCGAGGACACTC

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSAPTKPHARPQGAGNSVPNTVKPATQFPSKPAVAKLERVSPEQLTGAQSVIRSLEELDVEVIFGIPGGAVLLVYDPLFY
SKKLRHVLVRHEQGAGHAASGYAHVTGKVGVCMATSGPGATNLVTPLADAQMDSVPVVAITGQVGRSLIGTDAFQEADIS
GITMPITKHNFLVRAGDDIPRVLAEAFHIASSGRPGAVLVDIPKDVLQGQCKFSWPPKMDLPGYKPNTKPHNRQIRAAAK
LIADARKPVLYVGGGVIRGEATEQLRDLAELTGIPVVSTLMARGAFPDSHHQNLGMPGMHGTVAAVAALQRSDLLIALGT
RFDDRVTGKLDSFAPDAKVIHADIDPAEIGKNRHADVPIVGDVKAVIVELIAMLRHYEVPGNIEMTDWWSYLDGVRKTYP
LSYSPQSDGTLSPEYVIEKLGEIVGPEAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKIARPEAE
VWAIDGDGCFQMTNQELATCAIEGAPIKVALINNGNLGMVRQWQALFYQERYSQTDLATHSHRIPDFVKLAEALGCVGLR
CECEEDVVDVINQARAINNRPVVIDFIVGADAQVWPMVAAGASNDEIQAARGIRPLFDDESEGHV

Sequences:

>Translated_625_residues
MSAPTKPHARPQGAGNSVPNTVKPATQFPSKPAVAKLERVSPEQLTGAQSVIRSLEELDVEVIFGIPGGAVLLVYDPLFY
SKKLRHVLVRHEQGAGHAASGYAHVTGKVGVCMATSGPGATNLVTPLADAQMDSVPVVAITGQVGRSLIGTDAFQEADIS
GITMPITKHNFLVRAGDDIPRVLAEAFHIASSGRPGAVLVDIPKDVLQGQCKFSWPPKMDLPGYKPNTKPHNRQIRAAAK
LIADARKPVLYVGGGVIRGEATEQLRDLAELTGIPVVSTLMARGAFPDSHHQNLGMPGMHGTVAAVAALQRSDLLIALGT
RFDDRVTGKLDSFAPDAKVIHADIDPAEIGKNRHADVPIVGDVKAVIVELIAMLRHYEVPGNIEMTDWWSYLDGVRKTYP
LSYSPQSDGTLSPEYVIEKLGEIVGPEAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKIARPEAE
VWAIDGDGCFQMTNQELATCAIEGAPIKVALINNGNLGMVRQWQALFYQERYSQTDLATHSHRIPDFVKLAEALGCVGLR
CECEEDVVDVINQARAINNRPVVIDFIVGADAQVWPMVAAGASNDEIQAARGIRPLFDDESEGHV
>Mature_624_residues
SAPTKPHARPQGAGNSVPNTVKPATQFPSKPAVAKLERVSPEQLTGAQSVIRSLEELDVEVIFGIPGGAVLLVYDPLFYS
KKLRHVLVRHEQGAGHAASGYAHVTGKVGVCMATSGPGATNLVTPLADAQMDSVPVVAITGQVGRSLIGTDAFQEADISG
ITMPITKHNFLVRAGDDIPRVLAEAFHIASSGRPGAVLVDIPKDVLQGQCKFSWPPKMDLPGYKPNTKPHNRQIRAAAKL
IADARKPVLYVGGGVIRGEATEQLRDLAELTGIPVVSTLMARGAFPDSHHQNLGMPGMHGTVAAVAALQRSDLLIALGTR
FDDRVTGKLDSFAPDAKVIHADIDPAEIGKNRHADVPIVGDVKAVIVELIAMLRHYEVPGNIEMTDWWSYLDGVRKTYPL
SYSPQSDGTLSPEYVIEKLGEIVGPEAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKIARPEAEV
WAIDGDGCFQMTNQELATCAIEGAPIKVALINNGNLGMVRQWQALFYQERYSQTDLATHSHRIPDFVKLAEALGCVGLRC
ECEEDVVDVINQARAINNRPVVIDFIVGADAQVWPMVAAGASNDEIQAARGIRPLFDDESEGHV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=27.5261324041812, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI21361361, Length=600, Percent_Identity=26.5, Blast_Score=158, Evalue=2e-38,
Organism=Escherichia coli, GI87081685, Length=579, Percent_Identity=45.4231433506045, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1790104, Length=567, Percent_Identity=43.7389770723104, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=35.6756756756757, Blast_Score=334, Evalue=1e-92,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=28.2142857142857, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788716, Length=563, Percent_Identity=26.4653641207815, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=584, Percent_Identity=25.6849315068493, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=584, Percent_Identity=25.6849315068493, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=559, Percent_Identity=27.3703041144902, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=616, Percent_Identity=41.8831168831169, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320816, Length=494, Percent_Identity=23.4817813765182, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323163, Length=506, Percent_Identity=22.7272727272727, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=503, Percent_Identity=22.6640159045726, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321524, Length=499, Percent_Identity=22.6452905811623, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=26.5734265734266, Blast_Score=186, Evalue=4e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): ILVB_MYCLE (O33112)

Other databases:

- EMBL:   Z99263
- EMBL:   AL583923
- PIR:   T45413
- RefSeq:   NP_302166.1
- ProteinModelPortal:   O33112
- SMR:   O33112
- EnsemblBacteria:   EBMYCT00000029089
- GeneID:   910798
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1696
- NMPDR:   fig|272631.1.peg.1038
- Leproma:   ML1696
- GeneTree:   EBGT00050000014643
- HOGENOM:   HBG323037
- OMA:   RLVFIDI
- ProtClustDB:   PRK07789
- BioCyc:   MLEP272631:ML1696-MONOMER
- BRENDA:   2.2.1.6
- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00118

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 67021; Mature: 66890

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 92-92 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTKPHARPQGAGNSVPNTVKPATQFPSKPAVAKLERVSPEQLTGAQSVIRSLEELDV
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCE
EVIFGIPGGAVLLVYDPLFYSKKLRHVLVRHEQGAGHAASGYAHVTGKVGVCMATSGPGA
EEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCC
TNLVTPLADAQMDSVPVVAITGQVGRSLIGTDAFQEADISGITMPITKHNFLVRAGDDIP
CCCCCCCCCCCCCCCCEEEEECHHHHHHHCCCCHHHCCCCCEEEEEECCCEEEECCCHHH
RVLAEAFHIASSGRPGAVLVDIPKDVLQGQCKFSWPPKMDLPGYKPNTKPHNRQIRAAAK
HHHHHHHHHHCCCCCCEEEEECCHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
LIADARKPVLYVGGGVIRGEATEQLRDLAELTGIPVVSTLMARGAFPDSHHQNLGMPGMH
HHHHCCCCEEEECCEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
GTVAAVAALQRSDLLIALGTRFDDRVTGKLDSFAPDAKVIHADIDPAEIGKNRHADVPIV
HHHHHHHHHHHCCEEEEECCCCCCCCCCCHHCCCCCCEEEEECCCHHHCCCCCCCCCCEE
GDVKAVIVELIAMLRHYEVPGNIEMTDWWSYLDGVRKTYPLSYSPQSDGTLSPEYVIEKL
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
GEIVGPEAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKIARPEAE
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCE
VWAIDGDGCFQMTNQELATCAIEGAPIKVALINNGNLGMVRQWQALFYQERYSQTDLATH
EEEECCCCCEEECCCHHEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
SHRIPDFVKLAEALGCVGLRCECEEDVVDVINQARAINNRPVVIDFIVGADAQVWPMVAA
CCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEC
GASNDEIQAARGIRPLFDDESEGHV
CCCCCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAPTKPHARPQGAGNSVPNTVKPATQFPSKPAVAKLERVSPEQLTGAQSVIRSLEELDV
CCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCE
EVIFGIPGGAVLLVYDPLFYSKKLRHVLVRHEQGAGHAASGYAHVTGKVGVCMATSGPGA
EEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCC
TNLVTPLADAQMDSVPVVAITGQVGRSLIGTDAFQEADISGITMPITKHNFLVRAGDDIP
CCCCCCCCCCCCCCCCEEEEECHHHHHHHCCCCHHHCCCCCEEEEEECCCEEEECCCHHH
RVLAEAFHIASSGRPGAVLVDIPKDVLQGQCKFSWPPKMDLPGYKPNTKPHNRQIRAAAK
HHHHHHHHHHCCCCCCEEEEECCHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
LIADARKPVLYVGGGVIRGEATEQLRDLAELTGIPVVSTLMARGAFPDSHHQNLGMPGMH
HHHHCCCCEEEECCEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
GTVAAVAALQRSDLLIALGTRFDDRVTGKLDSFAPDAKVIHADIDPAEIGKNRHADVPIV
HHHHHHHHHHHCCEEEEECCCCCCCCCCCHHCCCCCCEEEEECCCHHHCCCCCCCCCCEE
GDVKAVIVELIAMLRHYEVPGNIEMTDWWSYLDGVRKTYPLSYSPQSDGTLSPEYVIEKL
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
GEIVGPEAVYVAGVGQHQMWAAQFISYEKPRTWLNSGGLGTMGFAIPAAMGAKIARPEAE
HHHCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCE
VWAIDGDGCFQMTNQELATCAIEGAPIKVALINNGNLGMVRQWQALFYQERYSQTDLATH
EEEECCCCCEEECCCHHEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHH
SHRIPDFVKLAEALGCVGLRCECEEDVVDVINQARAINNRPVVIDFIVGADAQVWPMVAA
CCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEC
GASNDEIQAARGIRPLFDDESEGHV
CCCCCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002