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The map label for this gene is yqhE [C]

Identifier: 221230361

GI number: 221230361

Start: 2012983

End: 2013831

Strand: Direct

Name: yqhE [C]

Synonym: MLBr_01669

Alternate gene names: 221230361

Gene position: 2012983-2013831 (Clockwise)

Preceding gene: 221230352

Following gene: 221230365

Centisome position: 61.6

GC content: 57.83

Gene sequence:

>849_bases
ATGATGCCGAGCACCCCCCGCACCTCTATACCCTCAATAACCCTTAACGACGAGAACACTATGCCGTTGCTCGGCCTTGG
CGTCGCGGAATTGTCGGAGGACGAGACCGAGCGCGCGGTTTTGGCAGCGCTGGAAATCGGCTGCCGGCTCATCGATACCG
CAGCAGCTTATGGCAACGAAGCCGCAGTCAGCCGCGCAATTGCCGCCTCCGGAATTCCCCGCGCCCAGTTGTTCGTCACC
ACCAAGTTGGCTACCCCCGACCAGGGTTTCACCAAGTCCCAAGATGCCTGCAACGCCAGCTTGGATCGACTTGGCATGGA
CTACGTCGACCTCTACCTGATTCACTGGCCGGCCCCGCCAGTGGGCCAGTATGTTGATGCCTGGGGAGGCATGATCCAAT
CCCGTGGCGAAGGCCATGCCCGCTCGATCGGCGTGTGCAATTTCACCGAGGAGCATTTATCGGCGATTATCGATCTCACC
TTTGTCACGCCAGCGGTCAACCAGATCGAGCTACACCCACTGCTCAACCAAGACGAGATGCGCAAATCAAATGCACAGCA
CAACGTTATCACCCAGTCCTACACCCCATTGGTTCTTGGCCGGTTGATGGACAATTCGACCCTGACCGCGATCGCCGCCG
AATATGGCAAAACACCTGCACAAGTACTGCTTCGGTGGAACTTGCAGCTGGGTAATGCAGTAGTCTTCCGGTCCGCCAAA
GCCGAGCACATTGCCAGCAACTTCGACGTCTTCGATTTCGAATTGGCCGTCAATCACATGGACGCAATGAATGAGTTGCA
CGACGGCACCCGGTTGCGCCCAGATCCAGAAACCTACGCCGGCAGCTGA

Upstream 100 bases:

>100_bases
TTCAAACTCAGCGCCGCGGAAATGGCATCGATCTCCTCGATCGATGACCGAACCCGGCTTGAACGCAACCCATGCACCTT
CGATTTCACAGGCAGGTGAA

Downstream 100 bases:

>100_bases
TCTCGCCCCGTAACTGCTACCCGACCTGACAAGGTCACTACAACTTACAGGTAGTACGCTCATCGACACCCTCGTCTACC
GGTAGTTGGTAACCAGCATA

Product: putative oxidoreductase of Aldo/keto reductase family

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MMPSTPRTSIPSITLNDENTMPLLGLGVAELSEDETERAVLAALEIGCRLIDTAAAYGNEAAVSRAIAASGIPRAQLFVT
TKLATPDQGFTKSQDACNASLDRLGMDYVDLYLIHWPAPPVGQYVDAWGGMIQSRGEGHARSIGVCNFTEEHLSAIIDLT
FVTPAVNQIELHPLLNQDEMRKSNAQHNVITQSYTPLVLGRLMDNSTLTAIAAEYGKTPAQVLLRWNLQLGNAVVFRSAK
AEHIASNFDVFDFELAVNHMDAMNELHDGTRLRPDPETYAGS

Sequences:

>Translated_282_residues
MMPSTPRTSIPSITLNDENTMPLLGLGVAELSEDETERAVLAALEIGCRLIDTAAAYGNEAAVSRAIAASGIPRAQLFVT
TKLATPDQGFTKSQDACNASLDRLGMDYVDLYLIHWPAPPVGQYVDAWGGMIQSRGEGHARSIGVCNFTEEHLSAIIDLT
FVTPAVNQIELHPLLNQDEMRKSNAQHNVITQSYTPLVLGRLMDNSTLTAIAAEYGKTPAQVLLRWNLQLGNAVVFRSAK
AEHIASNFDVFDFELAVNHMDAMNELHDGTRLRPDPETYAGS
>Mature_282_residues
MMPSTPRTSIPSITLNDENTMPLLGLGVAELSEDETERAVLAALEIGCRLIDTAAAYGNEAAVSRAIAASGIPRAQLFVT
TKLATPDQGFTKSQDACNASLDRLGMDYVDLYLIHWPAPPVGQYVDAWGGMIQSRGEGHARSIGVCNFTEEHLSAIIDLT
FVTPAVNQIELHPLLNQDEMRKSNAQHNVITQSYTPLVLGRLMDNSTLTAIAAEYGKTPAQVLLRWNLQLGNAVVFRSAK
AEHIASNFDVFDFELAVNHMDAMNELHDGTRLRPDPETYAGS

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family

Homologues:

Organism=Homo sapiens, GI24497577, Length=292, Percent_Identity=29.7945205479452, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI5174391, Length=292, Percent_Identity=29.7945205479452, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=32.1428571428571, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI310109920, Length=298, Percent_Identity=30.8724832214765, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI45446745, Length=298, Percent_Identity=30.8724832214765, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI4503285, Length=298, Percent_Identity=30.8724832214765, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI93277124, Length=306, Percent_Identity=31.3725490196078, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI5453543, Length=299, Percent_Identity=30.4347826086957, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI24497585, Length=298, Percent_Identity=31.2080536912752, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=32.6388888888889, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=28.7625418060201, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=30.0341296928328, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI223468663, Length=283, Percent_Identity=28.2685512367491, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=29.2418772563177, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI291291012, Length=294, Percent_Identity=28.2312925170068, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI300116271, Length=279, Percent_Identity=28.673835125448, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=30.3225806451613, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI207028673, Length=112, Percent_Identity=38.3928571428571, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI310109926, Length=112, Percent_Identity=38.3928571428571, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI87082198, Length=271, Percent_Identity=46.1254612546125, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1786400, Length=257, Percent_Identity=36.5758754863813, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1788081, Length=265, Percent_Identity=29.0566037735849, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=34.9442379182156, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17552492, Length=263, Percent_Identity=32.319391634981, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17537075, Length=301, Percent_Identity=29.5681063122924, Blast_Score=140, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17537077, Length=296, Percent_Identity=28.7162162162162, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17566692, Length=297, Percent_Identity=30.976430976431, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17564128, Length=302, Percent_Identity=30.1324503311258, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17550248, Length=296, Percent_Identity=31.4189189189189, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17537079, Length=301, Percent_Identity=25.5813953488372, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17561298, Length=277, Percent_Identity=27.4368231046931, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17561300, Length=259, Percent_Identity=28.1853281853282, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17562292, Length=278, Percent_Identity=26.2589928057554, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320576, Length=306, Percent_Identity=32.6797385620915, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324694, Length=294, Percent_Identity=33.6734693877551, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6322556, Length=259, Percent_Identity=28.1853281853282, Blast_Score=131, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6320079, Length=293, Percent_Identity=30.0341296928328, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319625, Length=301, Percent_Identity=31.5614617940199, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321896, Length=315, Percent_Identity=27.6190476190476, Blast_Score=115, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=34.8275862068965, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI20129731, Length=293, Percent_Identity=34.1296928327645, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45553081, Length=297, Percent_Identity=34.006734006734, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24663317, Length=295, Percent_Identity=32.8813559322034, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24662789, Length=299, Percent_Identity=33.4448160535117, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21356425, Length=300, Percent_Identity=32, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=32, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=30.3125, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI281366140, Length=262, Percent_Identity=32.4427480916031, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24662781, Length=299, Percent_Identity=30.1003344481605, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24662785, Length=297, Percent_Identity=30.6397306397306, Blast_Score=117, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1669_MYCLB (B8ZS00)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503777.1
- ProteinModelPortal:   B8ZS00
- SMR:   B8ZS00
- EnsemblBacteria:   EBMYCT00000085757
- GeneID:   7325397
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017291
- HOGENOM:   HBG605727
- OMA:   HNVVTQS
- ProtClustDB:   CLSK792199
- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210
- Gene3D:   G3DSA:3.20.20.100
- PANTHER:   PTHR11732
- PIRSF:   PIRSF000097
- PRINTS:   PR00069

Pfam domain/function: PF00248 Aldo_ket_red; SSF51430 Aldo/ket_red

EC number: NA

Molecular weight: Translated: 30693; Mature: 30693

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1; PS00062 ALDOKETO_REDUCTASE_2; PS00063 ALDOKETO_REDUCTASE_3

Important sites: ACT_SITE 57-57 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPSTPRTSIPSITLNDENTMPLLGLGVAELSEDETERAVLAALEIGCRLIDTAAAYGNE
CCCCCCCCCCCEEEECCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
AAVSRAIAASGIPRAQLFVTTKLATPDQGFTKSQDACNASLDRLGMDYVDLYLIHWPAPP
HHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCC
VGQYVDAWGGMIQSRGEGHARSIGVCNFTEEHLSAIIDLTFVTPAVNQIELHPLLNQDEM
HHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
RKSNAQHNVITQSYTPLVLGRLMDNSTLTAIAAEYGKTPAQVLLRWNLQLGNAVVFRSAK
HHCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHCCCHHHEEEEECCEECCEEEEECCC
AEHIASNFDVFDFELAVNHMDAMNELHDGTRLRPDPETYAGS
HHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MMPSTPRTSIPSITLNDENTMPLLGLGVAELSEDETERAVLAALEIGCRLIDTAAAYGNE
CCCCCCCCCCCEEEECCCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
AAVSRAIAASGIPRAQLFVTTKLATPDQGFTKSQDACNASLDRLGMDYVDLYLIHWPAPP
HHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCC
VGQYVDAWGGMIQSRGEGHARSIGVCNFTEEHLSAIIDLTFVTPAVNQIELHPLLNQDEM
HHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHH
RKSNAQHNVITQSYTPLVLGRLMDNSTLTAIAAEYGKTPAQVLLRWNLQLGNAVVFRSAK
HHCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHCCCHHHEEEEECCEECCEEEEECCC
AEHIASNFDVFDFELAVNHMDAMNELHDGTRLRPDPETYAGS
HHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA