Click here to switch to the map view.

The map label for this gene is panB

Identifier: 221230340

GI number: 221230340

Start: 1969054

End: 1969914

Strand: Direct

Name: panB

Synonym: MLBr_01635

Alternate gene names: 221230340

Gene position: 1969054-1969914 (Clockwise)

Preceding gene: 221230333

Following gene: 221230344

Centisome position: 60.25

GC content: 58.65

Gene sequence:

>861_bases
ATGTCTGAGCAGAATGTTAATGTTTATGGGGCTGATCCTTCTACATCCTCCATTCAATCAATACAGCGCACCAAAATACG
CACCAAGCATCTCCAAAAAATGAAGGCCGAAGGCCACAAGTGGGCCATGCTGACAGCCTATGATTATTCGACTGCTCGGG
TTTTCGACGAAGCCGGTATCCCGGTGTTGCTGGTGGGTGATTCAGCGGCCAACGTGGTGTACGGCTACGACACCACGGTG
CCGGTTTCGGCCGACGAACTACTCCCGCTGGTTCGCGGCGTGGTGCGGGGTGCGGGGCACGCTCTGGTCGTCGCCGACCT
GCCATTCGGCAGCTACGAACCCGGACCCACTGCCGCGCTGGCTGTCGCCACCCGATTTATGAAGGAGGGCGGTGCACACG
CCGTAAAACTCGAAGGCGGCCAGCGGGTAGCTGAACAAATCGCGTGCCTGACCGCAGCAGGCATTCCGGTAATGGCGCAC
ATCGGATTCACACCGCAAAGCGTCAACAGCCTCGGCGGTTTCCGGGTACAGGGCCGCGGCGGCGACGCCGAACAAACCGT
CGCTGATGCCGTTGCCGTTGCTGAAGCCGGAGCATTTTCCGTCGTAATGGAAATGGTGCCGACCGAGTTAGCCACCCAGA
TCACCGGCAAATTAACCATTCCGACAATCGGGATCGGTGCGGGTCTTAACTGCGACGCCCAGGTCTTAGTTTGGCAAGAT
ATGGCCGGTTTAAGCAGCGGTAAGGTGGCACGTTTCGTCAAGCAGTATGCTGATATCGCTGGCGAGTTGCGGCGTGCCGC
AATGCAGTACGCAGAGGAAGTGGCCAGCGCGGTTTTCCCTGCTGAGGAGCACTGCTTCTGA

Upstream 100 bases:

>100_bases
GATGGCAGACTATACAGGGTCAGACGAACCCGGGGACCCTACCGAACCAGCTTCTTCAGGGCCACGAGAAGCGAACCCGA
CCTAACCACAAAGGCCAGTG

Downstream 100 bases:

>100_bases
AAAGCTAGCCGCGTCTCCGCGCAATCTCTCTAAAGTCGTTGGCCGACAAGTGTTTCCACACCGACTAGCAGGTCTTTTAG
GTGTGGTCAGGATCGCAAAA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSEQNVNVYGADPSTSSIQSIQRTKIRTKHLQKMKAEGHKWAMLTAYDYSTARVFDEAGIPVLLVGDSAANVVYGYDTTV
PVSADELLPLVRGVVRGAGHALVVADLPFGSYEPGPTAALAVATRFMKEGGAHAVKLEGGQRVAEQIACLTAAGIPVMAH
IGFTPQSVNSLGGFRVQGRGGDAEQTVADAVAVAEAGAFSVVMEMVPTELATQITGKLTIPTIGIGAGLNCDAQVLVWQD
MAGLSSGKVARFVKQYADIAGELRRAAMQYAEEVASAVFPAEEHCF

Sequences:

>Translated_286_residues
MSEQNVNVYGADPSTSSIQSIQRTKIRTKHLQKMKAEGHKWAMLTAYDYSTARVFDEAGIPVLLVGDSAANVVYGYDTTV
PVSADELLPLVRGVVRGAGHALVVADLPFGSYEPGPTAALAVATRFMKEGGAHAVKLEGGQRVAEQIACLTAAGIPVMAH
IGFTPQSVNSLGGFRVQGRGGDAEQTVADAVAVAEAGAFSVVMEMVPTELATQITGKLTIPTIGIGAGLNCDAQVLVWQD
MAGLSSGKVARFVKQYADIAGELRRAAMQYAEEVASAVFPAEEHCF
>Mature_285_residues
SEQNVNVYGADPSTSSIQSIQRTKIRTKHLQKMKAEGHKWAMLTAYDYSTARVFDEAGIPVLLVGDSAANVVYGYDTTVP
VSADELLPLVRGVVRGAGHALVVADLPFGSYEPGPTAALAVATRFMKEGGAHAVKLEGGQRVAEQIACLTAAGIPVMAHI
GFTPQSVNSLGGFRVQGRGGDAEQTVADAVAVAEAGAFSVVMEMVPTELATQITGKLTIPTIGIGAGLNCDAQVLVWQDM
AGLSSGKVARFVKQYADIAGELRRAAMQYAEEVASAVFPAEEHCF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=257, Percent_Identity=45.9143968871595, Blast_Score=219, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319653, Length=270, Percent_Identity=38.1481481481481, Blast_Score=174, Evalue=2e-44,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_MYCLE (Q9CBT0)

Other databases:

- EMBL:   AL583922
- PIR:   E87113
- RefSeq:   NP_302126.1
- ProteinModelPortal:   Q9CBT0
- SMR:   Q9CBT0
- EnsemblBacteria:   EBMYCT00000028975
- GeneID:   909845
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1635
- NMPDR:   fig|272631.1.peg.998
- Leproma:   ML1635
- GeneTree:   EBGT00050000015555
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   MLEP272631:ML1635-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30012; Mature: 29880

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: ACT_SITE 204-204 BINDING 106-106 BINDING 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQNVNVYGADPSTSSIQSIQRTKIRTKHLQKMKAEGHKWAMLTAYDYSTARVFDEAGI
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHCCCC
PVLLVGDSAANVVYGYDTTVPVSADELLPLVRGVVRGAGHALVVADLPFGSYEPGPTAAL
EEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
AVATRFMKEGGAHAVKLEGGQRVAEQIACLTAAGIPVMAHIGFTPQSVNSLGGFRVQGRG
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCEEEECCC
GDAEQTVADAVAVAEAGAFSVVMEMVPTELATQITGKLTIPTIGIGAGLNCDAQVLVWQD
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEECEEECCCCCCCCCEEEEEEC
MAGLSSGKVARFVKQYADIAGELRRAAMQYAEEVASAVFPAEEHCF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
SEQNVNVYGADPSTSSIQSIQRTKIRTKHLQKMKAEGHKWAMLTAYDYSTARVFDEAGI
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHCCCC
PVLLVGDSAANVVYGYDTTVPVSADELLPLVRGVVRGAGHALVVADLPFGSYEPGPTAAL
EEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHH
AVATRFMKEGGAHAVKLEGGQRVAEQIACLTAAGIPVMAHIGFTPQSVNSLGGFRVQGRG
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCEEEECCC
GDAEQTVADAVAVAEAGAFSVVMEMVPTELATQITGKLTIPTIGIGAGLNCDAQVLVWQD
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEECEEECCCCCCCCCEEEEEEC
MAGLSSGKVARFVKQYADIAGELRRAAMQYAEEVASAVFPAEEHCF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002