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The map label for this gene is efpA [H]

Identifier: 221230300

GI number: 221230300

Start: 1886219

End: 1887823

Strand: Reverse

Name: efpA [H]

Synonym: MLBr_01562

Alternate gene names: 221230300

Gene position: 1887823-1886219 (Counterclockwise)

Preceding gene: 221230302

Following gene: 221230297

Centisome position: 57.77

GC content: 61.0

Gene sequence:

>1605_bases
ATGGTGACTCTTTACGACCATGAGCGGGCAGTCCACAACTGGACAGCTGCACGATCGGATCGTCCGGCCCCGTCGCGACT
GACGCAGCAGGTGGAGCCCGCCTCAGAACGCACAAGCAAGTACCCGACGTTGCTGCCTTCTGGTCGTTTCCCTTCTGGTC
GTTTCATTGCAGCGGTCATCGTGATCGGTGGCATGCAGCTATTGGCGACCATGGACAGCACCGTCGCCATCGTCGCGCTT
CCCAAGATTCAAAACGAGCTGAGCCTGTCCGACGCCGGCCGTAGTTGGGGGATTACTGCCTACGTGCTGACATTCGGCGG
GCTGATGTTGCTCGGTGGCCGCCTCGGCGACACCATCGGGCGCAAGCGCACGTTCATCGTCGGCGTCGCGCTGTTTACTA
TTTCCTCGGTGCTTTGCGCTGTGGCCTGGGACGAAGTGACCTTGGTCATTGCTCGACTATTGCAGGGCATCGGGTCGGCT
ATCGCATCACCGACCGGTCTCGCACTGGTTGCAACCACCTTCTCTAAGGGCTCAGCGCGCAATACCGCTACGGCGGTGTT
CGCCGCGATGACGGCAATCGGCTCGGTGATGGGCCTCGTGGTCGGCGGGGCGCTTACCGAGGTGTCGTGGCGACTGGCGT
TTTTAGTGAACGTGCCGATTGGGTTGGTGATGATCTACCTGGCCCGCACCGCTTTGCATGAGACCCACAAGGAGCGGATG
AAGCTGGACGCGGCTGGGGCCATTCTGGCCACGCTGGCTTGCACCGCGGCCGTTTTCGCCTTCTCGATGGGACCGGAGAA
GGGTTGGATTTCGCTGACCACGATCGGTTCGGGCTTGGTGGCATTGGTAGCCGCTATCGCATTTGCCGTCGTGGAACGTA
CTGCTGAAAATCCTGTCGTACCGTTTGACCTGTTCCGGGACCGTAATCGGCTGGTCACCTTCATCGCGATTTTCCTGGCC
GGTGGCGTCATTTTCAGCTTGACTGTATCCATCGGCCTATACATACAGGACATCTTGGGCTACAGCGCGCTACGCGCTGG
CATCGGCTTTATCCCGTTCGTCATCGCGATGGGAATCGGCCTGGGCGTGTCTTCACAGTTGGTGTCTCGGTTTTCGCCAC
GGGTATTGACGATAGGTGGTGGATGGCTGCTCATGGTCGCTATGCTGTACGGTTGGGCGTTCATGAACCGCGGCGTTCCC
TATTTTCCCAACCTGTTAGCACCCATCGTCGTTGGTGGAATCGGCATCGGCATGGCCGTCGTCCCGCTGACGTTGTCTGC
TATCGCCGGGGTAGGTTTCGATCGGATCGGCCCGGTGTCCGCGATTGCGCTAATGCTGCAGAGCCTCGGCGGGCCACTCG
TGCTGGCTGTCATCCAAGCGGTCATCACCTCGCGCACGCTGTACCTGGGCGGTACCACCGGCCCAGTGAAGTTTATGAAC
GACGCGCAGTTGCGTGCACTTGATCACGGCTACACCTACGGCCTGCTGTGGGTGGCCGGAGCGGTCGTCATCGTCGGCGT
GGCGGCGCTGTTCATCGGGTACACCCCCGAGCAGGTGGCCTACGCGCAGGAGGTCAAGGAAGCGGTCGACGCCGGCGAAC
TGTAG

Upstream 100 bases:

>100_bases
TCCGTGCAGAAGGGATCTGCCCTCGCGCGCTTACTGTCATTGGGCTGTCGCGGCATTCGGCCATTAAACTTCTCTTAAGA
TTACTGTAGGGTAACCGGCT

Downstream 100 bases:

>100_bases
GCCGTCGTCGGGGTGCTACCCGCGCTATTCGTTGCTGCCAGGGAAGGTGGTGGTGATGGGCCAGGCGCCCAGCATTTTGT
TCCATCGGGCGGCCATGACT

Product: putative transmembrane efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux protein A [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MVTLYDHERAVHNWTAARSDRPAPSRLTQQVEPASERTSKYPTLLPSGRFPSGRFIAAVIVIGGMQLLATMDSTVAIVAL
PKIQNELSLSDAGRSWGITAYVLTFGGLMLLGGRLGDTIGRKRTFIVGVALFTISSVLCAVAWDEVTLVIARLLQGIGSA
IASPTGLALVATTFSKGSARNTATAVFAAMTAIGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMIYLARTALHETHKERM
KLDAAGAILATLACTAAVFAFSMGPEKGWISLTTIGSGLVALVAAIAFAVVERTAENPVVPFDLFRDRNRLVTFIAIFLA
GGVIFSLTVSIGLYIQDILGYSALRAGIGFIPFVIAMGIGLGVSSQLVSRFSPRVLTIGGGWLLMVAMLYGWAFMNRGVP
YFPNLLAPIVVGGIGIGMAVVPLTLSAIAGVGFDRIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMN
DAQLRALDHGYTYGLLWVAGAVVIVGVAALFIGYTPEQVAYAQEVKEAVDAGEL

Sequences:

>Translated_534_residues
MVTLYDHERAVHNWTAARSDRPAPSRLTQQVEPASERTSKYPTLLPSGRFPSGRFIAAVIVIGGMQLLATMDSTVAIVAL
PKIQNELSLSDAGRSWGITAYVLTFGGLMLLGGRLGDTIGRKRTFIVGVALFTISSVLCAVAWDEVTLVIARLLQGIGSA
IASPTGLALVATTFSKGSARNTATAVFAAMTAIGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMIYLARTALHETHKERM
KLDAAGAILATLACTAAVFAFSMGPEKGWISLTTIGSGLVALVAAIAFAVVERTAENPVVPFDLFRDRNRLVTFIAIFLA
GGVIFSLTVSIGLYIQDILGYSALRAGIGFIPFVIAMGIGLGVSSQLVSRFSPRVLTIGGGWLLMVAMLYGWAFMNRGVP
YFPNLLAPIVVGGIGIGMAVVPLTLSAIAGVGFDRIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMN
DAQLRALDHGYTYGLLWVAGAVVIVGVAALFIGYTPEQVAYAQEVKEAVDAGEL
>Mature_534_residues
MVTLYDHERAVHNWTAARSDRPAPSRLTQQVEPASERTSKYPTLLPSGRFPSGRFIAAVIVIGGMQLLATMDSTVAIVAL
PKIQNELSLSDAGRSWGITAYVLTFGGLMLLGGRLGDTIGRKRTFIVGVALFTISSVLCAVAWDEVTLVIARLLQGIGSA
IASPTGLALVATTFSKGSARNTATAVFAAMTAIGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMIYLARTALHETHKERM
KLDAAGAILATLACTAAVFAFSMGPEKGWISLTTIGSGLVALVAAIAFAVVERTAENPVVPFDLFRDRNRLVTFIAIFLA
GGVIFSLTVSIGLYIQDILGYSALRAGIGFIPFVIAMGIGLGVSSQLVSRFSPRVLTIGGGWLLMVAMLYGWAFMNRGVP
YFPNLLAPIVVGGIGIGMAVVPLTLSAIAGVGFDRIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMN
DAQLRALDHGYTYGLLWVAGAVVIVGVAALFIGYTPEQVAYAQEVKEAVDAGEL

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=406, Percent_Identity=26.1083743842365, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790195, Length=308, Percent_Identity=31.1688311688312, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1789042, Length=413, Percent_Identity=26.3922518159806, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081983, Length=416, Percent_Identity=26.6826923076923, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788392, Length=347, Percent_Identity=27.6657060518732, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790146, Length=313, Percent_Identity=25.5591054313099, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081723, Length=134, Percent_Identity=35.8208955223881, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323520, Length=301, Percent_Identity=25.2491694352159, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324954, Length=317, Percent_Identity=25.8675078864353, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=315, Percent_Identity=26.3492063492064, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323935, Length=301, Percent_Identity=24.9169435215947, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=139, Percent_Identity=33.8129496402878, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55990; Mature: 55990

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLYDHERAVHNWTAARSDRPAPSRLTQQVEPASERTSKYPTLLPSGRFPSGRFIAAVI
CEEEECCHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHH
VIGGMQLLATMDSTVAIVALPKIQNELSLSDAGRSWGITAYVLTFGGLMLLGGRLGDTIG
HHHHHHHHHHCCCCEEEEEECHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHC
RKRTFIVGVALFTISSVLCAVAWDEVTLVIARLLQGIGSAIASPTGLALVATTFSKGSAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
NTATAVFAAMTAIGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMIYLARTALHETHKERM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
KLDAAGAILATLACTAAVFAFSMGPEKGWISLTTIGSGLVALVAAIAFAVVERTAENPVV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
PFDLFRDRNRLVTFIAIFLAGGVIFSLTVSIGLYIQDILGYSALRAGIGFIPFVIAMGIG
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LGVSSQLVSRFSPRVLTIGGGWLLMVAMLYGWAFMNRGVPYFPNLLAPIVVGGIGIGMAV
CCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VPLTLSAIAGVGFDRIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMN
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCEECC
DAQLRALDHGYTYGLLWVAGAVVIVGVAALFIGYTPEQVAYAQEVKEAVDAGEL
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVTLYDHERAVHNWTAARSDRPAPSRLTQQVEPASERTSKYPTLLPSGRFPSGRFIAAVI
CEEEECCHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHHH
VIGGMQLLATMDSTVAIVALPKIQNELSLSDAGRSWGITAYVLTFGGLMLLGGRLGDTIG
HHHHHHHHHHCCCCEEEEEECHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHC
RKRTFIVGVALFTISSVLCAVAWDEVTLVIARLLQGIGSAIASPTGLALVATTFSKGSAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
NTATAVFAAMTAIGSVMGLVVGGALTEVSWRLAFLVNVPIGLVMIYLARTALHETHKERM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
KLDAAGAILATLACTAAVFAFSMGPEKGWISLTTIGSGLVALVAAIAFAVVERTAENPVV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCC
PFDLFRDRNRLVTFIAIFLAGGVIFSLTVSIGLYIQDILGYSALRAGIGFIPFVIAMGIG
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
LGVSSQLVSRFSPRVLTIGGGWLLMVAMLYGWAFMNRGVPYFPNLLAPIVVGGIGIGMAV
CCCHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VPLTLSAIAGVGFDRIGPVSAIALMLQSLGGPLVLAVIQAVITSRTLYLGGTTGPVKFMN
HHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCEECC
DAQLRALDHGYTYGLLWVAGAVVIVGVAALFIGYTPEQVAYAQEVKEAVDAGEL
CCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]