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The map label for this gene is ansP2 [H]

Identifier: 221230152

GI number: 221230152

Start: 1554188

End: 1555705

Strand: Reverse

Name: ansP2 [H]

Synonym: MLBr_01305

Alternate gene names: 221230152

Gene position: 1555705-1554188 (Counterclockwise)

Preceding gene: 221230153

Following gene: 221230151

Centisome position: 47.6

GC content: 59.49

Gene sequence:

>1518_bases
ATGGCCACACTGGCTGAGAGCCCCGAACCAAAATCCGGCGCGTCGAGAGCTGGTGTCCTCGGCGAGGAGGCTGGTTACCA
CAAGGGGCTCAAACCTCGACAACTGCAGATGATCGGGATCGGCGGCGCGATCGGTACCGGGTTGTTCCTCGGCGCCGGCG
GCCGGCTGGCCAAGGCCGGGCCCGGATTGTTCCTGGTGTATGCCGTCTGCGGGGTGTTCGTCTTCCTGATACTGCGCGCA
CTGGGCGAGCTGGTGCTGCATCGTCCTTCATCGGGATCCTTCGTGTCTTACGCACGTGAGTTCTTCGGCGAGAAAGCCGC
CTACGTCGTCGGCTGGTTGTACTTCCTGGACTGGGCGATGACAGCGATCGTGGACACCACTGCCATCGCCACCTACCTGC
ATCGCTGGACAATCTTCACGGCGTTGCCACAATGGACTCTTGCGCTGCTCGCCCTGGCGGTGGTGTTGGTAATGAACCTG
ATCTCCGTGGAATGGTTCGGCGAGCTCGAATTTTGGGCCGCGCTGATCAAGGTTTGCGCGCTGATGGCATTTTTGGTCGT
AGGAACTATCTTCCTCGGCGGGCGTTACCCGGTCGACGGGCACAACACTGGGTTAAGCCTCTGGACTAGTCACGGCGGAT
TGTTCCCCACCGGCGTAGCGCCGCTAATAGTTGTCAGCTCCGGAGTGATGTTCGCCTACGCTGCCGTCGAACTGGTCGGC
ACCGCAGCAGGTGAAACCGTCGAGCCGAAAAAAATCATGCCGCGAGCGATCAACTCGGTAATCGCTCGCATCGCGATCTT
CTACGTCGGATCGGTGATTCTGCTGGCATTGTTGCTGCCCTACAGCGCATTCAAGGCCAGCGAAAGCCCGTTCGTCACGT
TCTTCTCCAAGGTTGGGTTCTACGGCGCCGGCGATCTGATGAACATCGTAGTGCTCACCGCCGCGCTGTCCAGCCTCAAC
GCCGGACTGTACGCAACCGGCCGTGTCATGCACTCGATCGCGATAAATGGTAGCGGACCGAAGTTCACCGCGCGAATGTC
GAAAAATGGTGTGCCATATGGCGGAATTCTTTTAGCCGCCGTCATCTGCCTGTGCGGTGTCGCACTAAACGCCTTCAACC
CCGGTCAAGCCTTCGAGATCGTGCTCAGCGTCGCCGCACTGGGCATCATCGCCGGGTGGGGAACAATCGTACTGTGCCAG
CTTCGACTACACAAGATGGCCAAAGCCGGGATCATGCGCCGACCCCGCTTTCGCATGCCGCTGGCCCCATACAGTGGCTA
CCTCACTTTGGCCTTCTTGTTCGCGGTACTGGTTGTGATGGCGTTCGACAAACCGATCGGCACCTGGACAGTAGCGTCAC
TGATCGTTATCGTCCCAGCCTTGATTGCAGGCTGGTACTCAATACGCAAGCGCGTGATGACCATCGCCCGTGAAAGAATG
GGCTATACCGGACCTTTCCCGGCGATCGCCAACCCACCGGTGCAACCATCAGAACGATCCCATAGCCAGAATCCGTAG

Upstream 100 bases:

>100_bases
GTCATCCGTTTGAGTAGGAAAAGATCGCTAGCTAACCCTGTCTTCACGATTCGCGGCAAGCCCACCGAACTCCGGTAGGA
CAACGATTAGGACCCCATGT

Downstream 100 bases:

>100_bases
ATCAACACAGTTACTAGGCCTATTGCGGTCGAGTTCCCCTAACCTAAGAAATCAGTACAACCTCACGACCAAATTGCACA
CTAACCCTGTCTTCACGATT

Product: putative L-asparagine permease

Products: Proton [Cytoplasm]; L-asparagine [Cytoplasm] [C]

Alternate protein names: L-asparagine transport protein 1 [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MATLAESPEPKSGASRAGVLGEEAGYHKGLKPRQLQMIGIGGAIGTGLFLGAGGRLAKAGPGLFLVYAVCGVFVFLILRA
LGELVLHRPSSGSFVSYAREFFGEKAAYVVGWLYFLDWAMTAIVDTTAIATYLHRWTIFTALPQWTLALLALAVVLVMNL
ISVEWFGELEFWAALIKVCALMAFLVVGTIFLGGRYPVDGHNTGLSLWTSHGGLFPTGVAPLIVVSSGVMFAYAAVELVG
TAAGETVEPKKIMPRAINSVIARIAIFYVGSVILLALLLPYSAFKASESPFVTFFSKVGFYGAGDLMNIVVLTAALSSLN
AGLYATGRVMHSIAINGSGPKFTARMSKNGVPYGGILLAAVICLCGVALNAFNPGQAFEIVLSVAALGIIAGWGTIVLCQ
LRLHKMAKAGIMRRPRFRMPLAPYSGYLTLAFLFAVLVVMAFDKPIGTWTVASLIVIVPALIAGWYSIRKRVMTIARERM
GYTGPFPAIANPPVQPSERSHSQNP

Sequences:

>Translated_505_residues
MATLAESPEPKSGASRAGVLGEEAGYHKGLKPRQLQMIGIGGAIGTGLFLGAGGRLAKAGPGLFLVYAVCGVFVFLILRA
LGELVLHRPSSGSFVSYAREFFGEKAAYVVGWLYFLDWAMTAIVDTTAIATYLHRWTIFTALPQWTLALLALAVVLVMNL
ISVEWFGELEFWAALIKVCALMAFLVVGTIFLGGRYPVDGHNTGLSLWTSHGGLFPTGVAPLIVVSSGVMFAYAAVELVG
TAAGETVEPKKIMPRAINSVIARIAIFYVGSVILLALLLPYSAFKASESPFVTFFSKVGFYGAGDLMNIVVLTAALSSLN
AGLYATGRVMHSIAINGSGPKFTARMSKNGVPYGGILLAAVICLCGVALNAFNPGQAFEIVLSVAALGIIAGWGTIVLCQ
LRLHKMAKAGIMRRPRFRMPLAPYSGYLTLAFLFAVLVVMAFDKPIGTWTVASLIVIVPALIAGWYSIRKRVMTIARERM
GYTGPFPAIANPPVQPSERSHSQNP
>Mature_504_residues
ATLAESPEPKSGASRAGVLGEEAGYHKGLKPRQLQMIGIGGAIGTGLFLGAGGRLAKAGPGLFLVYAVCGVFVFLILRAL
GELVLHRPSSGSFVSYAREFFGEKAAYVVGWLYFLDWAMTAIVDTTAIATYLHRWTIFTALPQWTLALLALAVVLVMNLI
SVEWFGELEFWAALIKVCALMAFLVVGTIFLGGRYPVDGHNTGLSLWTSHGGLFPTGVAPLIVVSSGVMFAYAAVELVGT
AAGETVEPKKIMPRAINSVIARIAIFYVGSVILLALLLPYSAFKASESPFVTFFSKVGFYGAGDLMNIVVLTAALSSLNA
GLYATGRVMHSIAINGSGPKFTARMSKNGVPYGGILLAAVICLCGVALNAFNPGQAFEIVLSVAALGIIAGWGTIVLCQL
RLHKMAKAGIMRRPRFRMPLAPYSGYLTLAFLFAVLVVMAFDKPIGTWTVASLIVIVPALIAGWYSIRKRVMTIARERMG
YTGPFPAIANPPVQPSERSHSQNP

Specific function: Unknown

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI181337167, Length=343, Percent_Identity=23.3236151603499, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI87081915, Length=475, Percent_Identity=58.3157894736842, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI1790653, Length=456, Percent_Identity=35.3070175438597, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1786302, Length=424, Percent_Identity=37.2641509433962, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1786789, Length=439, Percent_Identity=35.0797266514806, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI48994972, Length=422, Percent_Identity=38.1516587677725, Blast_Score=275, Evalue=7e-75,
Organism=Escherichia coli, GI1786602, Length=455, Percent_Identity=33.6263736263736, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI87081708, Length=431, Percent_Identity=34.8027842227378, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788480, Length=467, Percent_Identity=32.5481798715203, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789017, Length=429, Percent_Identity=33.1002331002331, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6324061, Length=462, Percent_Identity=30.5194805194805, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6320772, Length=440, Percent_Identity=29.7727272727273, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322892, Length=452, Percent_Identity=30.5309734513274, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324990, Length=446, Percent_Identity=27.8026905829596, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324059, Length=463, Percent_Identity=29.3736501079914, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321053, Length=423, Percent_Identity=26.241134751773, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324924, Length=477, Percent_Identity=26.8343815513627, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324553, Length=398, Percent_Identity=29.1457286432161, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6322967, Length=414, Percent_Identity=27.0531400966184, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321629, Length=425, Percent_Identity=26.3529411764706, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6320717, Length=403, Percent_Identity=26.0545905707196, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319824, Length=404, Percent_Identity=26.2376237623762, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6324981, Length=395, Percent_Identity=27.8481012658228, Blast_Score=126, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6319608, Length=455, Percent_Identity=25.2747252747253, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319543, Length=401, Percent_Identity=26.6832917705736, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320251, Length=419, Percent_Identity=23.1503579952267, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319542, Length=409, Percent_Identity=24.9388753056235, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320364, Length=489, Percent_Identity=23.5173824130879, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 53803; Mature: 53672

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLAESPEPKSGASRAGVLGEEAGYHKGLKPRQLQMIGIGGAIGTGLFLGAGGRLAKAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCC
PGLFLVYAVCGVFVFLILRALGELVLHRPSSGSFVSYAREFFGEKAAYVVGWLYFLDWAM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
TAIVDTTAIATYLHRWTIFTALPQWTLALLALAVVLVMNLISVEWFGELEFWAALIKVCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LMAFLVVGTIFLGGRYPVDGHNTGLSLWTSHGGLFPTGVAPLIVVSSGVMFAYAAVELVG
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
TAAGETVEPKKIMPRAINSVIARIAIFYVGSVILLALLLPYSAFKASESPFVTFFSKVGF
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
YGAGDLMNIVVLTAALSSLNAGLYATGRVMHSIAINGSGPKFTARMSKNGVPYGGILLAA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCEEEECCCCCCCHHHHHHHH
VICLCGVALNAFNPGQAFEIVLSVAALGIIAGWGTIVLCQLRLHKMAKAGIMRRPRFRMP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LAPYSGYLTLAFLFAVLVVMAFDKPIGTWTVASLIVIVPALIAGWYSIRKRVMTIARERM
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYTGPFPAIANPPVQPSERSHSQNP
CCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATLAESPEPKSGASRAGVLGEEAGYHKGLKPRQLQMIGIGGAIGTGLFLGAGGRLAKAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCC
PGLFLVYAVCGVFVFLILRALGELVLHRPSSGSFVSYAREFFGEKAAYVVGWLYFLDWAM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
TAIVDTTAIATYLHRWTIFTALPQWTLALLALAVVLVMNLISVEWFGELEFWAALIKVCA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LMAFLVVGTIFLGGRYPVDGHNTGLSLWTSHGGLFPTGVAPLIVVSSGVMFAYAAVELVG
HHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
TAAGETVEPKKIMPRAINSVIARIAIFYVGSVILLALLLPYSAFKASESPFVTFFSKVGF
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
YGAGDLMNIVVLTAALSSLNAGLYATGRVMHSIAINGSGPKFTARMSKNGVPYGGILLAA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCEEEECCCCCCCHHHHHHHH
VICLCGVALNAFNPGQAFEIVLSVAALGIIAGWGTIVLCQLRLHKMAKAGIMRRPRFRMP
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LAPYSGYLTLAFLFAVLVVMAFDKPIGTWTVASLIVIVPALIAGWYSIRKRVMTIARERM
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYTGPFPAIANPPVQPSERSHSQNP
CCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-asparagine [Periplasm] + Proton [Periplasm] = Proton [Cytoplasm] + L-asparagine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]