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The map label for this gene is ansA

Identifier: 221230086

GI number: 221230086

Start: 1416049

End: 1416981

Strand: Reverse

Name: ansA

Synonym: MLBr_01198

Alternate gene names: 221230086

Gene position: 1416981-1416049 (Counterclockwise)

Preceding gene: 221230092

Following gene: 221230080

Centisome position: 43.36

GC content: 65.38

Gene sequence:

>933_bases
ATGAGCCGCCTCACCGTCATCGCCACTGGAGGTACCATATCGACCAGTACCGACCACGATGGGGTGCGCCGCCCCGCCTA
CAGCGGAACGGAACTGACCGCACACGTGGCCGTCGATGTCGACGTGGTGGACCTGATGACTGTCGACAGCTCACAGCTGA
TACCGACCGATTGGGATCGGATTCGCGCTGCAGTGCACACAGCGACCAGAAACGGCGCCAGGGGTGTGGTCGTCACCCAC
GGGACCGACACCATGGAAGAGACCGCACTGTGGCTGGACCTCACCTACGCGGGGAACGTCCCAGTAGTCTTGACCGGCGC
CATGCGCAGCGCCGACGCTCCCAATGCAGACGGTCCGACCAATCTGCGCGAAGCACTTGCGGTGGCGGCTAGTCCGGCCG
CTCGCGGCGTGGGCGTACTGGTGTCTTTCGCCGGCCGGGTATTACAACCGTTAGGCCTACGCAAGGCAGCTACGCAGGAT
CTGAGCGGCTTCGCCGGCGAGTTGCTCGGCACCAGCTCCAGCGGTTTTGCGCTGACAGCAGCCAAGACCCGCCCGTATCT
CGGGGACCTGTGCGCCGCCGATGCACCGCGAGTCGATATTGTCGCGGCATACTTGGGCAGTGATTCGGTGGCGTTGGACG
CTTATGTGTCCACCGGAGCGCGTGGCATCGTCCTGGAGGCACTGGGCTCGGGCAATGCCGGGGCCGCGGTAGTCGACGGG
GTACGCCGCCATTGCCACAATGGGATCGTGATCGCGGTGTCCACCCGCGTGCCCGAAGGCCATGTCAGCGCAGGCTACGG
TCCTGGCCACGACCTGGTAGAAGCCGGCGCGGTGCTGGTGCCATGGCTACGACCCTCGCAGGTCCGGGTGCTGTTGATGG
CTGCGCTGGCCGCAAACCTACCGATCGCTGACGTCATCGAACGCTGGGGCTAA

Upstream 100 bases:

>100_bases
GGTCCGTCAGGCACTCTACCCATCATCCCCCAAGGGTAATACTGTCCTACCGACCCCCGCGATCGATACGGACCGACCAA
TCGTGCAGGATGATCCCACC

Downstream 100 bases:

>100_bases
CGTACCCACCGAATCCTCGACCCACCACTTGACTATATAGTCCGACCAGTCGAGATAGTCCGACCAGTCGAGACTGTCAA
GCGGGTTGACTTTAACGACA

Product: L-asparaginase/L-glutaminase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSRLTVIATGGTISTSTDHDGVRRPAYSGTELTAHVAVDVDVVDLMTVDSSQLIPTDWDRIRAAVHTATRNGARGVVVTH
GTDTMEETALWLDLTYAGNVPVVLTGAMRSADAPNADGPTNLREALAVAASPAARGVGVLVSFAGRVLQPLGLRKAATQD
LSGFAGELLGTSSSGFALTAAKTRPYLGDLCAADAPRVDIVAAYLGSDSVALDAYVSTGARGIVLEALGSGNAGAAVVDG
VRRHCHNGIVIAVSTRVPEGHVSAGYGPGHDLVEAGAVLVPWLRPSQVRVLLMAALAANLPIADVIERWG

Sequences:

>Translated_310_residues
MSRLTVIATGGTISTSTDHDGVRRPAYSGTELTAHVAVDVDVVDLMTVDSSQLIPTDWDRIRAAVHTATRNGARGVVVTH
GTDTMEETALWLDLTYAGNVPVVLTGAMRSADAPNADGPTNLREALAVAASPAARGVGVLVSFAGRVLQPLGLRKAATQD
LSGFAGELLGTSSSGFALTAAKTRPYLGDLCAADAPRVDIVAAYLGSDSVALDAYVSTGARGIVLEALGSGNAGAAVVDG
VRRHCHNGIVIAVSTRVPEGHVSAGYGPGHDLVEAGAVLVPWLRPSQVRVLLMAALAANLPIADVIERWG
>Mature_309_residues
SRLTVIATGGTISTSTDHDGVRRPAYSGTELTAHVAVDVDVVDLMTVDSSQLIPTDWDRIRAAVHTATRNGARGVVVTHG
TDTMEETALWLDLTYAGNVPVVLTGAMRSADAPNADGPTNLREALAVAASPAARGVGVLVSFAGRVLQPLGLRKAATQDL
SGFAGELLGTSSSGFALTAAKTRPYLGDLCAADAPRVDIVAAYLGSDSVALDAYVSTGARGIVLEALGSGNAGAAVVDGV
RRHCHNGIVIAVSTRVPEGHVSAGYGPGHDLVEAGAVLVPWLRPSQVRVLLMAALAANLPIADVIERWG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family

Homologues:

Organism=Escherichia coli, GI1789327, Length=231, Percent_Identity=35.0649350649351, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788065, Length=241, Percent_Identity=27.8008298755187, Blast_Score=61, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320527, Length=329, Percent_Identity=27.6595744680851, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323189, Length=236, Percent_Identity=33.0508474576271, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323187, Length=236, Percent_Identity=33.0508474576271, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323186, Length=236, Percent_Identity=33.0508474576271, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323184, Length=236, Percent_Identity=33.0508474576271, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASPG_MYCLE (Q9X7E6)

Other databases:

- EMBL:   AL049478
- EMBL:   AL583921
- PIR:   H87058
- RefSeq:   NP_301872.1
- ProteinModelPortal:   Q9X7E6
- SMR:   Q9X7E6
- EnsemblBacteria:   EBMYCT00000029320
- GeneID:   910300
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1198
- NMPDR:   fig|272631.1.peg.744
- Leproma:   ML1198
- GeneTree:   EBGT00050000016418
- HOGENOM:   HBG497495
- OMA:   LAMVAMT
- ProtClustDB:   CLSK791234
- BioCyc:   MLEP272631:ML1198-MONOMER
- BRENDA:   3.5.1.1
- GO:   GO:0005737
- InterPro:   IPR006034
- InterPro:   IPR020827
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: =3.5.1.1

Molecular weight: Translated: 31798; Mature: 31667

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLTVIATGGTISTSTDHDGVRRPAYSGTELTAHVAVDVDVVDLMTVDSSQLIPTDWDR
CCCEEEEEECCEEECCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEECCCCCCCCCHHH
IRAAVHTATRNGARGVVVTHGTDTMEETALWLDLTYAGNVPVVLTGAMRSADAPNADGPT
HHHHHHHHCCCCCCEEEEECCCCCHHHEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCH
NLREALAVAASPAARGVGVLVSFAGRVLQPLGLRKAATQDLSGFAGELLGTSSSGFALTA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
AKTRPYLGDLCAADAPRVDIVAAYLGSDSVALDAYVSTGARGIVLEALGSGNAGAAVVDG
ECCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEHHCCCCEEEEEECCCCCCCHHHHHH
VRRHCHNGIVIAVSTRVPEGHVSAGYGPGHDLVEAGAVLVPWLRPSQVRVLLMAALAANL
HHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCCC
PIADVIERWG
CHHHHHHHCC
>Mature Secondary Structure 
SRLTVIATGGTISTSTDHDGVRRPAYSGTELTAHVAVDVDVVDLMTVDSSQLIPTDWDR
CCEEEEEECCEEECCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEECCCCCCCCCHHH
IRAAVHTATRNGARGVVVTHGTDTMEETALWLDLTYAGNVPVVLTGAMRSADAPNADGPT
HHHHHHHHCCCCCCEEEEECCCCCHHHEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCH
NLREALAVAASPAARGVGVLVSFAGRVLQPLGLRKAATQDLSGFAGELLGTSSSGFALTA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
AKTRPYLGDLCAADAPRVDIVAAYLGSDSVALDAYVSTGARGIVLEALGSGNAGAAVVDG
ECCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEHHCCCCEEEEEECCCCCCCHHHHHH
VRRHCHNGIVIAVSTRVPEGHVSAGYGPGHDLVEAGAVLVPWLRPSQVRVLLMAALAANL
HHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCCC
PIADVIERWG
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002