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The map label for this gene is ogt

Identifier: 221230059

GI number: 221230059

Start: 1346400

End: 1346897

Strand: Reverse

Name: ogt

Synonym: MLBr_01151

Alternate gene names: 221230059

Gene position: 1346897-1346400 (Counterclockwise)

Preceding gene: 221230061

Following gene: 221230057

Centisome position: 41.21

GC content: 54.82

Gene sequence:

>498_bases
ATGATTCATTGCCGTAGCATGGACAGCCCAATCGGACCGTTGATCCTAGCCGGGCACGGTTCGGTCTTGACGAATCTACA
GATGGTCGATCAGACCTACGAACCCAGTCGCGTCGGCTGGCTACCCGAGAACGGTGCATTTGCTGAGGCCGTCAGCCAAC
TTGATGCCTACTTCGCCGGCGAACTTACCGAATTCGCTATTGAGTTGGATCTATGTGGAACAGAATTCCAACAACGAGTC
TGGCAGGCTCTGCTGACAATTCCTTATGGGGAAACCCGGTCGTACGGAGAGATCGCAGAACAGGTCGGGGCTCCTGGCGC
TGCGCGTGCCGTGGGATTGGCCAACAGCAGAAATCCCATCGCCATTATTGTGCCCTGCCATCGAGTGATTGGCGCAAGCG
GACAATTGATCGGCTACGGCGGGGGACTCAACCGGAAGCTTACACTGCTCGAATTGGAGAAACACCAGGTACTGACAAGT
TTGACATTATTCGACTAG

Upstream 100 bases:

>100_bases
CACGTTGGCGTTCCTATGGCCACCCAACACCTCTGGACCACACCCTTCCTCCATCCAGTAAACCAATGGCCACTAAAAGA
ACATCCCGAAAGGGGGTTGC

Downstream 100 bases:

>100_bases
GCATGTGCAGAAAATTCAGGGAATAACAAAACCGCCTTTGACGAAAATGTTTTGTTTATGTTCGGCAGTGTCCTACTTTT
CCACCCGAATGGGCAGTATC

Product: putative methylated-DNA-protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIHCRSMDSPIGPLILAGHGSVLTNLQMVDQTYEPSRVGWLPENGAFAEAVSQLDAYFAGELTEFAIELDLCGTEFQQRV
WQALLTIPYGETRSYGEIAEQVGAPGAARAVGLANSRNPIAIIVPCHRVIGASGQLIGYGGGLNRKLTLLELEKHQVLTS
LTLFD

Sequences:

>Translated_165_residues
MIHCRSMDSPIGPLILAGHGSVLTNLQMVDQTYEPSRVGWLPENGAFAEAVSQLDAYFAGELTEFAIELDLCGTEFQQRV
WQALLTIPYGETRSYGEIAEQVGAPGAARAVGLANSRNPIAIIVPCHRVIGASGQLIGYGGGLNRKLTLLELEKHQVLTS
LTLFD
>Mature_165_residues
MIHCRSMDSPIGPLILAGHGSVLTNLQMVDQTYEPSRVGWLPENGAFAEAVSQLDAYFAGELTEFAIELDLCGTEFQQRV
WQALLTIPYGETRSYGEIAEQVGAPGAARAVGLANSRNPIAIIVPCHRVIGASGQLIGYGGGLNRKLTLLELEKHQVLTS
LTLFD

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

Organism=Homo sapiens, GI197304670, Length=167, Percent_Identity=33.5329341317365, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1787596, Length=108, Percent_Identity=51.8518518518518, Blast_Score=109, Evalue=7e-26,
Organism=Escherichia coli, GI1788542, Length=112, Percent_Identity=46.4285714285714, Blast_Score=100, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=52.5, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=52.5, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=89, Percent_Identity=43.8202247191011, Blast_Score=88, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17137554, Length=86, Percent_Identity=40.6976744186046, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_MYCLE (P52982)

Other databases:

- EMBL:   U00014
- EMBL:   AL583920
- PIR:   S72810
- RefSeq:   NP_301845.1
- ProteinModelPortal:   P52982
- SMR:   P52982
- EnsemblBacteria:   EBMYCT00000029011
- GeneID:   910248
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1151
- NMPDR:   fig|272631.1.peg.717
- Leproma:   ML1151
- GeneTree:   EBGT00050000016345
- HOGENOM:   HBG752398
- OMA:   THARAVG
- ProtClustDB:   CLSK791095
- BioCyc:   MLEP272631:ML1151-MONOMER
- BRENDA:   2.1.1.63
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 17796; Mature: 17796

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHCRSMDSPIGPLILAGHGSVLTNLQMVDQTYEPSRVGWLPENGAFAEAVSQLDAYFAG
CEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
ELTEFAIELDLCGTEFQQRVWQALLTIPYGETRSYGEIAEQVGAPGAARAVGLANSRNPI
CHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHEEECCCCCCCE
AIIVPCHRVIGASGQLIGYGGGLNRKLTLLELEKHQVLTSLTLFD
EEEEECHHHHCCCCCEEEECCCCCCEEEEEEEHHHHHHHHHCCCC
>Mature Secondary Structure
MIHCRSMDSPIGPLILAGHGSVLTNLQMVDQTYEPSRVGWLPENGAFAEAVSQLDAYFAG
CEECCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
ELTEFAIELDLCGTEFQQRVWQALLTIPYGETRSYGEIAEQVGAPGAARAVGLANSRNPI
CHHHEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHEEECCCCCCCE
AIIVPCHRVIGASGQLIGYGGGLNRKLTLLELEKHQVLTSLTLFD
EEEEECHHHHCCCCCEEEECCCCCCEEEEEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002