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The map label for this gene is ag45

Identifier: 221229978

GI number: 221229978

Start: 1199277

End: 1200566

Strand: Reverse

Name: ag45

Synonym: MLBr_01036

Alternate gene names: 221229978

Gene position: 1200566-1199277 (Counterclockwise)

Preceding gene: 221229979

Following gene: 221229977

Centisome position: 36.74

GC content: 56.36

Gene sequence:

>1290_bases
ATGAGTGTCATTGCGATCACCGTGTTCATCGTCACCTATGCACTAATCGCCAGCGATCGGGTCAACAAGACGCTGGCGGC
CCTGACGGGCGCGGCGATCGTGGTCACTTTGCCCATTATCAATTCCGAAGACGTCTTCTACTCCCATGAAACCGGAATCG
ATTGGGAAGTCATCTTTTTGCTGTTAAGCATGATGATCATCGTCAGTGTGCTACGCCAAACCGGTGTGTTCGAATACGTC
GCAATCTGGACCGCCAAACGCTCACATGGTTCCCCGCTGCGCATCCTGCTCCTACTGGTGCTAGTGATGGCGCTCGGGTC
GGCCTTACTGGACAACGTCACCACGGTGCTTTTGATTGCTCCGGTCACGCTGTTGGTGTGCGAGCGCCTGACAATCAATG
CGGCACCATTTCTGATGGCCGAAGTCTTCGCCTCCAACATCGGCGGCGCAGCGACGTTGGTCGGCGACCCGCCCAACATC
ATCATCGCCAGTCGAGCAGGATTCTCTTTCAACGACTTCCTAATCCACCTGACACCGATCGTGATCATCGTGACAGTAGT
GCTCAGTGCATTGCTGCCACGCCTTTTTCGCGGCGCATTCGCGGTCGATCCCGAGCGGGTAGCCGATATCATGTCACTGA
ACGAGCGTGAAGCAATCCGCGATCGCTGGCTGCTCATCAAATGCGGCGTTGTCTTGTTGCTGGTATTCGTAGCCTTCATC
GCCCATCCGGTGCTGCACACCGGGCCGTCCCTGGTGGGGATGCTGGGCGCCGGCATTCTGATCGTGATCTCCAAGTTGGA
GCGGTCCGATTACCTGTCCAGCGTCAAGTGGGAGACATTGCTGTTTTTCGCCGGCCTGTTCATTATGGTCGGGGCTTTGG
TGAAAACCGATGTGGTCAATCAGCTGGCACGGGCAACGACCACCTTGACTGGTGGACACGAATTACTCACCGTCGTCCTG
ACTCTCGGCGTCTCAACTCTAGTATCCAGTATCATAGACAACATTCCTTACGTCGCCACGATGACACCCATTGTCTCGGA
GCTGGTAGCATCCATGCCGGATCAAAGCCACACCGACATCCTATGGTGGGCGCTGGCGTTGGGCGCCGATTTCGGCGGCA
ACCTTACCGCCGTCGGCGCCAGTGCCAATGTCGTTATGCTTGAAATCGCCAAGAGCGCAGGAACTCCCATCTCGTTCTGG
GAATTCACCCGCAAAGGCATTGCGGTCACTGTGATCTCGATCGCTCTCGCCGGGATCTACTTATGGTTGCGTTACCTCGT
GATGAGCTGA

Upstream 100 bases:

>100_bases
GGTGTGATCACAGCATCGCGCCTTCTAAGAGCGGCCCTGAAACATTGATCCCGACCGATGCAGTGACAAGTCACCGGCTG
ACAGCAGCGCAGGAAGCTAG

Downstream 100 bases:

>100_bases
GCTCGGTTATTCAGGTGTGCTTGGCACGAAATGATCGAAACATCAGCCAGGAGATAGCGAAGTTGTAATCAAAACCAGCA
GCCAGATAAGGCCATAACAT

Product: putative membrane transport protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSVIAITVFIVTYALIASDRVNKTLAALTGAAIVVTLPIINSEDVFYSHETGIDWEVIFLLLSMMIIVSVLRQTGVFEYV
AIWTAKRSHGSPLRILLLLVLVMALGSALLDNVTTVLLIAPVTLLVCERLTINAAPFLMAEVFASNIGGAATLVGDPPNI
IIASRAGFSFNDFLIHLTPIVIIVTVVLSALLPRLFRGAFAVDPERVADIMSLNEREAIRDRWLLIKCGVVLLLVFVAFI
AHPVLHTGPSLVGMLGAGILIVISKLERSDYLSSVKWETLLFFAGLFIMVGALVKTDVVNQLARATTTLTGGHELLTVVL
TLGVSTLVSSIIDNIPYVATMTPIVSELVASMPDQSHTDILWWALALGADFGGNLTAVGASANVVMLEIAKSAGTPISFW
EFTRKGIAVTVISIALAGIYLWLRYLVMS

Sequences:

>Translated_429_residues
MSVIAITVFIVTYALIASDRVNKTLAALTGAAIVVTLPIINSEDVFYSHETGIDWEVIFLLLSMMIIVSVLRQTGVFEYV
AIWTAKRSHGSPLRILLLLVLVMALGSALLDNVTTVLLIAPVTLLVCERLTINAAPFLMAEVFASNIGGAATLVGDPPNI
IIASRAGFSFNDFLIHLTPIVIIVTVVLSALLPRLFRGAFAVDPERVADIMSLNEREAIRDRWLLIKCGVVLLLVFVAFI
AHPVLHTGPSLVGMLGAGILIVISKLERSDYLSSVKWETLLFFAGLFIMVGALVKTDVVNQLARATTTLTGGHELLTVVL
TLGVSTLVSSIIDNIPYVATMTPIVSELVASMPDQSHTDILWWALALGADFGGNLTAVGASANVVMLEIAKSAGTPISFW
EFTRKGIAVTVISIALAGIYLWLRYLVMS
>Mature_428_residues
SVIAITVFIVTYALIASDRVNKTLAALTGAAIVVTLPIINSEDVFYSHETGIDWEVIFLLLSMMIIVSVLRQTGVFEYVA
IWTAKRSHGSPLRILLLLVLVMALGSALLDNVTTVLLIAPVTLLVCERLTINAAPFLMAEVFASNIGGAATLVGDPPNII
IASRAGFSFNDFLIHLTPIVIIVTVVLSALLPRLFRGAFAVDPERVADIMSLNEREAIRDRWLLIKCGVVLLLVFVAFIA
HPVLHTGPSLVGMLGAGILIVISKLERSDYLSSVKWETLLFFAGLFIMVGALVKTDVVNQLARATTTLTGGHELLTVVLT
LGVSTLVSSIIDNIPYVATMTPIVSELVASMPDQSHTDILWWALALGADFGGNLTAVGASANVVMLEIAKSAGTPISFWE
FTRKGIAVTVISIALAGIYLWLRYLVMS

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CitM (TC 2.A.11) transporter family

Homologues:

Organism=Homo sapiens, GI157266326, Length=165, Percent_Identity=39.3939393939394, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI87082277, Length=388, Percent_Identity=23.7113402061856, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581759, Length=498, Percent_Identity=32.5301204819277, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI19920704, Length=498, Percent_Identity=32.9317269076305, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28574683, Length=498, Percent_Identity=32.9317269076305, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28574681, Length=498, Percent_Identity=32.9317269076305, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28574685, Length=498, Percent_Identity=32.9317269076305, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28574678, Length=498, Percent_Identity=32.9317269076305, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI45550608, Length=471, Percent_Identity=31.6348195329087, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI85724998, Length=475, Percent_Identity=25.8947368421053, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI62484377, Length=475, Percent_Identity=25.8947368421053, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI221472711, Length=220, Percent_Identity=32.2727272727273, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24656008, Length=481, Percent_Identity=26.4033264033264, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24581764, Length=220, Percent_Identity=32.2727272727273, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AG45_MYCLE (P46838)

Other databases:

- EMBL:   L10660
- EMBL:   U15181
- EMBL:   AL583920
- PIR:   F87038
- RefSeq:   NP_301764.1
- ProteinModelPortal:   P46838
- EnsemblBacteria:   EBMYCT00000029034
- GeneID:   910118
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML1036
- NMPDR:   fig|272631.1.peg.636
- Leproma:   ML1036
- GeneTree:   EBGT00050000016378
- HOGENOM:   HBG727257
- OMA:   IFFMSIF
- ProtClustDB:   CLSK792003
- BioCyc:   MLEP272631:ML1036-MONOMER
- InterPro:   IPR000802
- InterPro:   IPR004680
- PRINTS:   PR00758

Pfam domain/function: PF03600 CitMHS

EC number: NA

Molecular weight: Translated: 46110; Mature: 45978

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x21de47b0)-; HASH(0x29b64700)-; HASH(0x29b606a4)-; HASH(0x29b6cc14)-; HASH(0x29b12800)-; HASH(0x29bae554)-; HASH(0x29b613dc)-; HASH(0x29bb5bf8)-; HASH(0x29b582b0)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIAITVFIVTYALIASDRVNKTLAALTGAAIVVTLPIINSEDVFYSHETGIDWEVIFL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCEEECCCCCCHHHHHH
LLSMMIIVSVLRQTGVFEYVAIWTAKRSHGSPLRILLLLVLVMALGSALLDNVTTVLLIA
HHHHHHHHHHHHHCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVTLLVCERLTINAAPFLMAEVFASNIGGAATLVGDPPNIIIASRAGFSFNDFLIHLTPI
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHH
VIIVTVVLSALLPRLFRGAFAVDPERVADIMSLNEREAIRDRWLLIKCGVVLLLVFVAFI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AHPVLHTGPSLVGMLGAGILIVISKLERSDYLSSVKWETLLFFAGLFIMVGALVKTDVVN
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLARATTTLTGGHELLTVVLTLGVSTLVSSIIDNIPYVATMTPIVSELVASMPDQSHTDI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHH
LWWALALGADFGGNLTAVGASANVVMLEIAKSAGTPISFWEFTRKGIAVTVISIALAGIY
HHHHHHHCCCCCCCEEEECCCCCEEEEEEHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
LWLRYLVMS
HHHHHHHCH
>Mature Secondary Structure 
SVIAITVFIVTYALIASDRVNKTLAALTGAAIVVTLPIINSEDVFYSHETGIDWEVIFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCEEECCCCCCHHHHHH
LLSMMIIVSVLRQTGVFEYVAIWTAKRSHGSPLRILLLLVLVMALGSALLDNVTTVLLIA
HHHHHHHHHHHHHCCCHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PVTLLVCERLTINAAPFLMAEVFASNIGGAATLVGDPPNIIIASRAGFSFNDFLIHLTPI
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHH
VIIVTVVLSALLPRLFRGAFAVDPERVADIMSLNEREAIRDRWLLIKCGVVLLLVFVAFI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AHPVLHTGPSLVGMLGAGILIVISKLERSDYLSSVKWETLLFFAGLFIMVGALVKTDVVN
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLARATTTLTGGHELLTVVLTLGVSTLVSSIIDNIPYVATMTPIVSELVASMPDQSHTDI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHH
LWWALALGADFGGNLTAVGASANVVMLEIAKSAGTPISFWEFTRKGIAVTVISIALAGIY
HHHHHHHCCCCCCCEEEECCCCCEEEEEEHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
LWLRYLVMS
HHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002