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The map label for this gene is metE

Identifier: 221229937

GI number: 221229937

Start: 1127818

End: 1130100

Strand: Reverse

Name: metE

Synonym: MLBr_00961

Alternate gene names: 221229937

Gene position: 1130100-1127818 (Counterclockwise)

Preceding gene: 221229941

Following gene: 221229934

Centisome position: 34.58

GC content: 60.8

Gene sequence:

>2283_bases
ATGGACGAACTCGTGACCACTCAATCATTCACCGCAACCGTAACTGGCTCTCCACGCATTGGCCCGCGCCGCGAACTTAA
ACGGGCGACCGAAGGCTATTGGGCCAAGCGTACCAGCCGATCAGAACTGGAGTCCGTCGCCTCAACATTGCGCCGCGACA
TGTGGTCGGACTTAGCCGCCGCCGGCCTGGACTCCGTACCGGTGAACACCTTCTCTTACTACGACCAGATGCTCGACACG
GCATTCATGCTCGGCGCGCTGCCTGCCCGGGTAGCACAAGTGTCCGACGACCTAGATCAGTACTTCGCCCTCGCACGCGG
CAACAACGACATCAAGCCGCTGGAGATGACTAAGTGGTTCGACACCAACTACCACTACCTGGTTCCTGAAATCGAGCCCG
CGACCACCTTCTCACTGAACCCAGGCAAGATACTCGGTGAGCTGAAAGAAGCACTTGAGCAAAGAATTCCGTCCCGACCG
GTCATTATCGGTCCGGTCACCTTCCTGTTACTGAGCAAGGGCATCAATGGCGGGGGCGCACCGATACAGCGGCTCGAGGA
GCTGGTGGGAATCTACTGCACGCTGCTATCACTGCTCGCCGAGAATGGCGCACGATGGGTACAGTTCGACGAGCCGGCGC
TGGTGACTGATCTATCCCCCGATGCACCGGCGTTGGCGGAAGCAGTTTACACTGCACTCGGCTCAGTTAGCAAACGACCC
GCCATTTACGTGGCCACTTACTTCGGTAACCCCGGCGCTTCCTTGGCGGGGCTAGCCCGCACGCCCATCGAGGCGATCGG
TGTCGACTTCGTTTGTGGTGCCGACACGTCGGTCGCGGCGGTGCCCGAGCTGGCCGGCAAGACTCTGGTGGCTGGCATCG
TCGACGGACGCAACATCTGGCGCACTGACCTGGAATCGGCGTTGAGCAAGTTGGCTACTCTGCTGGGTTCAGCAGCCACC
GTTGCTGTTTCGACGTCGTGCTCTACGCTGCATGTGCCGTATTCGTTGGAACCAGAAACCGACCTGGACGACAATTTGCG
CAGCTGGCTGGCGTTCGGTGCGGAAAAGGTGGCCGAAGTCGTTGTGCTGGCACGCGCACTTCGCGACGGGCGCGACGCGG
TCGCCGATGAGATCGCGGCGTCCAATGCCGCCGTTGCCTCGCGACGCAGCGACCCGCGGCTGCACAACGGGCAGGTACGC
GCGCGTATTGACTCGATTGTCGCTTCCGGTACGCACCGCGGTGACGCAGCGCAGCGCCGCACCAGCCAGGACGCGCGCCT
ACACTTACCGCCGCTGCCGACCACGACGATCGGCTCCTACCCGCAGACCTCAGCGATCCGCAAAGCGCGAGCGGCACTGC
AGGACGCTGAGATCGACGAGGCCGAGTACATCAGCAGGATGAAAAAAGAAGTCGCCGACGCCATCAAACTGCAGGAGCAA
CTCGGGCTAGATGTACTGGTCCATGGCGAGCCGGAGCGCAACGACATGGTACAGTATTTCGCTGAGCAACTGGGCGGCTT
CTTCGCCACGCAGAACGGTTGGGTGCAGTCCTACGGCAGCCGTTGTGTACGTCCGCCGATCCTCTACGGTGACGTGTCCC
GGCCTCACCCGATGACAATCGAGTGGATCACCTACGCGCAGTCCCTAACTGACAAGCCAGTTAAGGGCATGTTGACCGGA
CCGGTCACGATCTTAGCCTGGTCGTTTGTTCGTGACGACCAGCCGCTGGCCGATACCGCGAACCAAGTAGCACTGGCGAT
TCGCGATGAGACCGTAGATCTACAATCCGCCGGTATCGCAATCATCCAGGTTGACGAGCCCGCGCTACGTGAGCTGCTGC
CGCTGCGTAGGGCTGATCAAGACGAATACTTATGTTGGGCAGTAAAGGCTTTCCGCCTAGCTACCTCGGGGGTCGCCGAC
TCGACGCAAATCCACACTCATCTGTGCTACTCCGAGTTCGGCGAAGTGATTGGAGCTATCGCCGACCTGGACGCCGACGT
CACATCCATCGAAGCGGCGCGCTCACACATGGAAGTATTGGATGACCTGAACGCAGTCGGCTTCGCTAACAGCATAGGCC
CGGGAGTCTACGACATCCACTCGCCGCGGGTACCAAGCACTGACGAGATTGCCAAGTCGCTACGCGCAGCATTAAAAGCC
ATACCGATGCAACGGCTTTGGGTTAACCCCGACTGCGGGCTGAAGACCCGATCAGTTGACGAGGTGAGCGCGTCGCTGCA
GAACATGGTCGCAGCAGCACGCCAGGTGCGGGCAGGGGCCTAA

Upstream 100 bases:

>100_bases
AAACCGGTGCGGTTATGTACCTGAAGTGCTGCTGCAATCGGATCGGCTGTGCGTCCGCTGGCGGTGACCACCCCGTCGAT
TTAAGGATGAAGTCGATCAA

Downstream 100 bases:

>100_bases
GCCATCCCAGAATCGTCGGCTAACTCGAACATCCCTGGGCCGGCACATCGTCGGCCCAGGGATGCTGGGTCAGCATGTCG
GCCACTGCACGACCACACTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MDELVTTQSFTATVTGSPRIGPRRELKRATEGYWAKRTSRSELESVASTLRRDMWSDLAAAGLDSVPVNTFSYYDQMLDT
AFMLGALPARVAQVSDDLDQYFALARGNNDIKPLEMTKWFDTNYHYLVPEIEPATTFSLNPGKILGELKEALEQRIPSRP
VIIGPVTFLLLSKGINGGGAPIQRLEELVGIYCTLLSLLAENGARWVQFDEPALVTDLSPDAPALAEAVYTALGSVSKRP
AIYVATYFGNPGASLAGLARTPIEAIGVDFVCGADTSVAAVPELAGKTLVAGIVDGRNIWRTDLESALSKLATLLGSAAT
VAVSTSCSTLHVPYSLEPETDLDDNLRSWLAFGAEKVAEVVVLARALRDGRDAVADEIAASNAAVASRRSDPRLHNGQVR
ARIDSIVASGTHRGDAAQRRTSQDARLHLPPLPTTTIGSYPQTSAIRKARAALQDAEIDEAEYISRMKKEVADAIKLQEQ
LGLDVLVHGEPERNDMVQYFAEQLGGFFATQNGWVQSYGSRCVRPPILYGDVSRPHPMTIEWITYAQSLTDKPVKGMLTG
PVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAIIQVDEPALRELLPLRRADQDEYLCWAVKAFRLATSGVAD
STQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNAVGFANSIGPGVYDIHSPRVPSTDEIAKSLRAALKA
IPMQRLWVNPDCGLKTRSVDEVSASLQNMVAAARQVRAGA

Sequences:

>Translated_760_residues
MDELVTTQSFTATVTGSPRIGPRRELKRATEGYWAKRTSRSELESVASTLRRDMWSDLAAAGLDSVPVNTFSYYDQMLDT
AFMLGALPARVAQVSDDLDQYFALARGNNDIKPLEMTKWFDTNYHYLVPEIEPATTFSLNPGKILGELKEALEQRIPSRP
VIIGPVTFLLLSKGINGGGAPIQRLEELVGIYCTLLSLLAENGARWVQFDEPALVTDLSPDAPALAEAVYTALGSVSKRP
AIYVATYFGNPGASLAGLARTPIEAIGVDFVCGADTSVAAVPELAGKTLVAGIVDGRNIWRTDLESALSKLATLLGSAAT
VAVSTSCSTLHVPYSLEPETDLDDNLRSWLAFGAEKVAEVVVLARALRDGRDAVADEIAASNAAVASRRSDPRLHNGQVR
ARIDSIVASGTHRGDAAQRRTSQDARLHLPPLPTTTIGSYPQTSAIRKARAALQDAEIDEAEYISRMKKEVADAIKLQEQ
LGLDVLVHGEPERNDMVQYFAEQLGGFFATQNGWVQSYGSRCVRPPILYGDVSRPHPMTIEWITYAQSLTDKPVKGMLTG
PVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAIIQVDEPALRELLPLRRADQDEYLCWAVKAFRLATSGVAD
STQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNAVGFANSIGPGVYDIHSPRVPSTDEIAKSLRAALKA
IPMQRLWVNPDCGLKTRSVDEVSASLQNMVAAARQVRAGA
>Mature_760_residues
MDELVTTQSFTATVTGSPRIGPRRELKRATEGYWAKRTSRSELESVASTLRRDMWSDLAAAGLDSVPVNTFSYYDQMLDT
AFMLGALPARVAQVSDDLDQYFALARGNNDIKPLEMTKWFDTNYHYLVPEIEPATTFSLNPGKILGELKEALEQRIPSRP
VIIGPVTFLLLSKGINGGGAPIQRLEELVGIYCTLLSLLAENGARWVQFDEPALVTDLSPDAPALAEAVYTALGSVSKRP
AIYVATYFGNPGASLAGLARTPIEAIGVDFVCGADTSVAAVPELAGKTLVAGIVDGRNIWRTDLESALSKLATLLGSAAT
VAVSTSCSTLHVPYSLEPETDLDDNLRSWLAFGAEKVAEVVVLARALRDGRDAVADEIAASNAAVASRRSDPRLHNGQVR
ARIDSIVASGTHRGDAAQRRTSQDARLHLPPLPTTTIGSYPQTSAIRKARAALQDAEIDEAEYISRMKKEVADAIKLQEQ
LGLDVLVHGEPERNDMVQYFAEQLGGFFATQNGWVQSYGSRCVRPPILYGDVSRPHPMTIEWITYAQSLTDKPVKGMLTG
PVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIAIIQVDEPALRELLPLRRADQDEYLCWAVKAFRLATSGVAD
STQIHTHLCYSEFGEVIGAIADLDADVTSIEAARSHMEVLDDLNAVGFANSIGPGVYDIHSPRVPSTDEIAKSLRAALKA
IPMQRLWVNPDCGLKTRSVDEVSASLQNMVAAARQVRAGA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=752, Percent_Identity=46.9414893617021, Blast_Score=674, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=768, Percent_Identity=45.9635416666667, Blast_Score=632, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_MYCLE (O05564)

Other databases:

- EMBL:   Z94723
- EMBL:   AL583920
- PIR:   C87029
- RefSeq:   NP_301723.1
- ProteinModelPortal:   O05564
- SMR:   O05564
- EnsemblBacteria:   EBMYCT00000028777
- GeneID:   909974
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0961
- NMPDR:   fig|272631.1.peg.595
- Leproma:   ML0961
- GeneTree:   EBGT00050000017657
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   MLEP272631:ML0961-MONOMER
- BRENDA:   2.1.1.14
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 82236; Mature: 82236

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELVTTQSFTATVTGSPRIGPRRELKRATEGYWAKRTSRSELESVASTLRRDMWSDLAA
CCCCEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AGLDSVPVNTFSYYDQMLDTAFMLGALPARVAQVSDDLDQYFALARGNNDIKPLEMTKWF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
DTNYHYLVPEIEPATTFSLNPGKILGELKEALEQRIPSRPVIIGPVTFLLLSKGINGGGA
CCCCEEECCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCH
PIQRLEELVGIYCTLLSLLAENGARWVQFDEPALVTDLSPDAPALAEAVYTALGSVSKRP
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCC
AIYVATYFGNPGASLAGLARTPIEAIGVDFVCGADTSVAAVPELAGKTLVAGIVDGRNIW
EEEEEEEECCCCCCHHHHHHCCHHHHCCEEEECCCCCHHHHHHHHCCEEEEEECCCCHHH
RTDLESALSKLATLLGSAATVAVSTSCSTLHVPYSLEPETDLDDNLRSWLAFGAEKVAEV
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCEECCCCCCCHHHHHHHHHHHHHHHHHH
VVLARALRDGRDAVADEIAASNAAVASRRSDPRLHNGQVRARIDSIVASGTHRGDAAQRR
HHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
TSQDARLHLPPLPTTTIGSYPQTSAIRKARAALQDAEIDEAEYISRMKKEVADAIKLQEQ
CCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGLDVLVHGEPERNDMVQYFAEQLGGFFATQNGWVQSYGSRCVRPPILYGDVSRPHPMTI
CCCEEEEECCCCHHHHHHHHHHHHCCEEECCCCHHHHHCCCCCCCCEEECCCCCCCCCEE
EWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIA
HHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCHHHCHHHEEEEEECCCEEHHHCCEE
IIQVDEPALRELLPLRRADQDEYLCWAVKAFRLATSGVADSTQIHTHLCYSEFGEVIGAI
EEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ADLDADVTSIEAARSHMEVLDDLNAVGFANSIGPGVYDIHSPRVPSTDEIAKSLRAALKA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHH
IPMQRLWVNPDCGLKTRSVDEVSASLQNMVAAARQVRAGA
CCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDELVTTQSFTATVTGSPRIGPRRELKRATEGYWAKRTSRSELESVASTLRRDMWSDLAA
CCCCEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AGLDSVPVNTFSYYDQMLDTAFMLGALPARVAQVSDDLDQYFALARGNNDIKPLEMTKWF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
DTNYHYLVPEIEPATTFSLNPGKILGELKEALEQRIPSRPVIIGPVTFLLLSKGINGGGA
CCCCEEECCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCH
PIQRLEELVGIYCTLLSLLAENGARWVQFDEPALVTDLSPDAPALAEAVYTALGSVSKRP
HHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCC
AIYVATYFGNPGASLAGLARTPIEAIGVDFVCGADTSVAAVPELAGKTLVAGIVDGRNIW
EEEEEEEECCCCCCHHHHHHCCHHHHCCEEEECCCCCHHHHHHHHCCEEEEEECCCCHHH
RTDLESALSKLATLLGSAATVAVSTSCSTLHVPYSLEPETDLDDNLRSWLAFGAEKVAEV
HHHHHHHHHHHHHHHCCCCEEEEECCCCEEECCEECCCCCCCHHHHHHHHHHHHHHHHHH
VVLARALRDGRDAVADEIAASNAAVASRRSDPRLHNGQVRARIDSIVASGTHRGDAAQRR
HHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHH
TSQDARLHLPPLPTTTIGSYPQTSAIRKARAALQDAEIDEAEYISRMKKEVADAIKLQEQ
CCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGLDVLVHGEPERNDMVQYFAEQLGGFFATQNGWVQSYGSRCVRPPILYGDVSRPHPMTI
CCCEEEEECCCCHHHHHHHHHHHHCCEEECCCCHHHHHCCCCCCCCEEECCCCCCCCCEE
EWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQSAGIA
HHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCHHHCHHHEEEEEECCCEEHHHCCEE
IIQVDEPALRELLPLRRADQDEYLCWAVKAFRLATSGVADSTQIHTHLCYSEFGEVIGAI
EEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ADLDADVTSIEAARSHMEVLDDLNAVGFANSIGPGVYDIHSPRVPSTDEIAKSLRAALKA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHH
IPMQRLWVNPDCGLKTRSVDEVSASLQNMVAAARQVRAGA
CCHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002