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The map label for this gene is asnB [H]

Identifier: 221229874

GI number: 221229874

Start: 1037649

End: 1039682

Strand: Reverse

Name: asnB [H]

Synonym: MLBr_00874

Alternate gene names: 221229874

Gene position: 1039682-1037649 (Counterclockwise)

Preceding gene: 221229879

Following gene: 221229872

Centisome position: 31.81

GC content: 60.87

Gene sequence:

>2034_bases
ATGGATGATCGGCGCCTACGAACATTGCCGAATGAGGCATACTGGATTGCCGTGTGTGGACTGCTGGCGTTTGTAGTGGC
TTCGGCGCGCGCTGATAGCCGCGGCTCTGGTGGGACCAACAACATTCTTCAGGCCGATCATTCCATGGCCCGCGCGGTAC
ATTTGATGCGTCACCGCGGGCCTGACGAGCTAAGCACCTGGTCCGACCCGGACATTGATGGGTTCTCGGGAGGGGTTGCG
TTTGGGTTTAACCGGTTGTCCATCATCGACATTGCGCATTCGCATCAGCCGCTGCGGTGGGGACCACCGGAGACACCCAA
CCGCTACATCCTGGTATTCAACGGCGAGATCTACAACTACCTGGAGCTGCGCGACGAGCTGGTCATCCGGCACGGCGCTG
CTTTCGCCACCGACGGAGACGGTGAATCTATCGTCGCCGGCTTCCATTACTGGGGCACCGAGATACTAACACGGCTGCGT
GGCATGTTCGCCTTCGCGCTGTGGGACACCATCGCCCACGAATTGTTCTGTGCCCGAGACCCTTTCGGGATCAAACCACT
GTTCATGGCGACCGGAACCAACGGCACCGCGGTGGCCAGCGAAAAGAAATGCCTGCTTGAACTGGCCGAAGTGATCAGAT
TCGACACGCAGATTGACACCCGCGCACTACAGCACTACACGGTCCTGCAGTACGTGCCCGAGCCCGAGACGCTGCATCGC
GGGGTGCACCGACTCGAATCTGGCTGTTACGCACGTATCCGTCCCGAACAGCTCGATCCAGCGACCACCCGCTACTTCGT
GCCGCAATTTGCCGCCACACCGATCACCAGCAGCACTGAACAGACCCGCTACGGCGAAATCACCGCAGTGCTTGCGGACT
CAGTGGCCAAGCACATGCGCGCCGACGTCACCGTTGGCTCGTTTCTGTCCGGCGGTATCGACTCCACAGCCATCGCAGCA
CTGGCGATCCGACACAATCCGCGGCTGATTACATTTACCACCGGCTTCGAGCATGAGGGATTCTCCGAGATCGACGTGGC
GGCTGCTTCGGCTGAAGCAATCGGTGCGCGCCACATCGTGAAGGTGGTTACCCCCAACGAGTTCGTCGCCGCCCTGCCGG
AGATCGTCTGGTATCTCGACGAGCCAATTGCCGACCCGGCCCTGGTTCCGCTGTTTTTCGTCGCCCGCGAAGCTCGCAAG
CACGTCAAGGTGGTGTTGTCCGGCGAGGGCGCCGACGAGCTGTTCGGCGGCTACAGAATCTATCGAGAGCCGCTGTCGTT
GAAGCCCTTCGACTACCTACCCGGGCCACTGCGACGCTCGATGGGCAAGGTGTCCAAATCTCTGCCCGAAGGTATGCGCG
GCAAGAATCTGCTACACCGCGGATCACTCACGCTCGAGGAACGCTACTACGGCAATGCCCGCAATTTCTCGGAAGCGCAG
TTGCAGGCTGTGCTACCCAGGTTCCACACGGGCTGGACCCACACCGACGTCACGGCAGCGGTGTACGCCGAATCCGTCGG
CTGGGACCCGGTAGCCCGCATGCAGCACATCGATCTGTTCACCTGGCTACGTGGCGACATTTTGGCCAAGGCCGACAAGA
TGACAATGGCCAATTCGCTGGAGCTGCGGGTACCGTTCCTTGATCCCGAAGTGTTCGCCGTGGCGTCCCGGTTACCCATG
CAGGCAAAGATCACCCGCACCACCACGAAATACGCACTGCGGCGCGCACTGGAATCCATCGTCCCGGCACACGTGTTGCA
TCGCCCCAAACTCGGGTTCCCGGTCCCAATCCGGCATTGGCTGCGCGCCGGCGAACTGCTGGAATGGGCGTACGCGCTGG
TGAACTCGTCGCAGGCCGAACATCTGGTCAACCTAGCCGCCGTGCACCGGATGCTTGACGAGCACCGAGGTGGCATCAGC
GATCATAGTCGCCGGCTGTGGACGGTGCTAATTTTTATGCTGTGGCACGCCATCTTCATCGAACACAGTGTGGTGCCACA
GATCAGCGAGCCACAGTATCCGGTACAGCCGTAA

Upstream 100 bases:

>100_bases
ACCCGCTTAAAGGCGGGATTCTTCGGCTAACTCGGATGTCTAATACTACGCAGCGTAGACCACGGCACTCGCCCAAGAGG
CGATACGGGTCCCAACGACG

Downstream 100 bases:

>100_bases
GTAACTAACCGGGCCGCAGTTAAGCCGGATGCGCGGATAAGGGTACAGCCATGCGATGAAGGCAATAATTTAATTTAAGG
TGACGACACCGAGCAGCTAG

Product: putative asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MDDRRLRTLPNEAYWIAVCGLLAFVVASARADSRGSGGTNNILQADHSMARAVHLMRHRGPDELSTWSDPDIDGFSGGVA
FGFNRLSIIDIAHSHQPLRWGPPETPNRYILVFNGEIYNYLELRDELVIRHGAAFATDGDGESIVAGFHYWGTEILTRLR
GMFAFALWDTIAHELFCARDPFGIKPLFMATGTNGTAVASEKKCLLELAEVIRFDTQIDTRALQHYTVLQYVPEPETLHR
GVHRLESGCYARIRPEQLDPATTRYFVPQFAATPITSSTEQTRYGEITAVLADSVAKHMRADVTVGSFLSGGIDSTAIAA
LAIRHNPRLITFTTGFEHEGFSEIDVAAASAEAIGARHIVKVVTPNEFVAALPEIVWYLDEPIADPALVPLFFVAREARK
HVKVVLSGEGADELFGGYRIYREPLSLKPFDYLPGPLRRSMGKVSKSLPEGMRGKNLLHRGSLTLEERYYGNARNFSEAQ
LQAVLPRFHTGWTHTDVTAAVYAESVGWDPVARMQHIDLFTWLRGDILAKADKMTMANSLELRVPFLDPEVFAVASRLPM
QAKITRTTTKYALRRALESIVPAHVLHRPKLGFPVPIRHWLRAGELLEWAYALVNSSQAEHLVNLAAVHRMLDEHRGGIS
DHSRRLWTVLIFMLWHAIFIEHSVVPQISEPQYPVQP

Sequences:

>Translated_677_residues
MDDRRLRTLPNEAYWIAVCGLLAFVVASARADSRGSGGTNNILQADHSMARAVHLMRHRGPDELSTWSDPDIDGFSGGVA
FGFNRLSIIDIAHSHQPLRWGPPETPNRYILVFNGEIYNYLELRDELVIRHGAAFATDGDGESIVAGFHYWGTEILTRLR
GMFAFALWDTIAHELFCARDPFGIKPLFMATGTNGTAVASEKKCLLELAEVIRFDTQIDTRALQHYTVLQYVPEPETLHR
GVHRLESGCYARIRPEQLDPATTRYFVPQFAATPITSSTEQTRYGEITAVLADSVAKHMRADVTVGSFLSGGIDSTAIAA
LAIRHNPRLITFTTGFEHEGFSEIDVAAASAEAIGARHIVKVVTPNEFVAALPEIVWYLDEPIADPALVPLFFVAREARK
HVKVVLSGEGADELFGGYRIYREPLSLKPFDYLPGPLRRSMGKVSKSLPEGMRGKNLLHRGSLTLEERYYGNARNFSEAQ
LQAVLPRFHTGWTHTDVTAAVYAESVGWDPVARMQHIDLFTWLRGDILAKADKMTMANSLELRVPFLDPEVFAVASRLPM
QAKITRTTTKYALRRALESIVPAHVLHRPKLGFPVPIRHWLRAGELLEWAYALVNSSQAEHLVNLAAVHRMLDEHRGGIS
DHSRRLWTVLIFMLWHAIFIEHSVVPQISEPQYPVQP
>Mature_677_residues
MDDRRLRTLPNEAYWIAVCGLLAFVVASARADSRGSGGTNNILQADHSMARAVHLMRHRGPDELSTWSDPDIDGFSGGVA
FGFNRLSIIDIAHSHQPLRWGPPETPNRYILVFNGEIYNYLELRDELVIRHGAAFATDGDGESIVAGFHYWGTEILTRLR
GMFAFALWDTIAHELFCARDPFGIKPLFMATGTNGTAVASEKKCLLELAEVIRFDTQIDTRALQHYTVLQYVPEPETLHR
GVHRLESGCYARIRPEQLDPATTRYFVPQFAATPITSSTEQTRYGEITAVLADSVAKHMRADVTVGSFLSGGIDSTAIAA
LAIRHNPRLITFTTGFEHEGFSEIDVAAASAEAIGARHIVKVVTPNEFVAALPEIVWYLDEPIADPALVPLFFVAREARK
HVKVVLSGEGADELFGGYRIYREPLSLKPFDYLPGPLRRSMGKVSKSLPEGMRGKNLLHRGSLTLEERYYGNARNFSEAQ
LQAVLPRFHTGWTHTDVTAAVYAESVGWDPVARMQHIDLFTWLRGDILAKADKMTMANSLELRVPFLDPEVFAVASRLPM
QAKITRTTTKYALRRALESIVPAHVLHRPKLGFPVPIRHWLRAGELLEWAYALVNSSQAEHLVNLAAVHRMLDEHRGGIS
DHSRRLWTVLIFMLWHAIFIEHSVVPQISEPQYPVQP

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=375, Percent_Identity=28.5333333333333, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI168229252, Length=375, Percent_Identity=28.5333333333333, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI168229250, Length=375, Percent_Identity=28.5333333333333, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI296010848, Length=375, Percent_Identity=28.5333333333333, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI296010852, Length=298, Percent_Identity=28.1879194630872, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI296010850, Length=298, Percent_Identity=28.1879194630872, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1786889, Length=382, Percent_Identity=28.7958115183246, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71993933, Length=429, Percent_Identity=27.972027972028, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=429, Percent_Identity=27.972027972028, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17560178, Length=379, Percent_Identity=29.5514511873351, Blast_Score=120, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321563, Length=413, Percent_Identity=27.3607748184019, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=412, Percent_Identity=27.1844660194175, Blast_Score=120, Evalue=9e-28,
Organism=Drosophila melanogaster, GI45553209, Length=384, Percent_Identity=29.9479166666667, Blast_Score=143, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 75742; Mature: 75742

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRRLRTLPNEAYWIAVCGLLAFVVASARADSRGSGGTNNILQADHSMARAVHLMRHRG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
PDELSTWSDPDIDGFSGGVAFGFNRLSIIDIAHSHQPLRWGPPETPNRYILVFNGEIYNY
CCCCCCCCCCCCCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHH
LELRDELVIRHGAAFATDGDGESIVAGFHYWGTEILTRLRGMFAFALWDTIAHELFCARD
HHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFGIKPLFMATGTNGTAVASEKKCLLELAEVIRFDTQIDTRALQHYTVLQYVPEPETLHR
CCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCHHHHHH
GVHRLESGCYARIRPEQLDPATTRYFVPQFAATPITSSTEQTRYGEITAVLADSVAKHMR
HHHHHCCCCEEEECCCCCCCCHHHEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ADVTVGSFLSGGIDSTAIAALAIRHNPRLITFTTGFEHEGFSEIDVAAASAEAIGARHIV
HCCCHHHHHCCCCCHHHHHHEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEE
KVVTPNEFVAALPEIVWYLDEPIADPALVPLFFVAREARKHVKVVLSGEGADELFGGYRI
EEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCHHE
YREPLSLKPFDYLPGPLRRSMGKVSKSLPEGMRGKNLLHRGSLTLEERYYGNARNFSEAQ
EECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEHHHHCCCCCCCHHHH
LQAVLPRFHTGWTHTDVTAAVYAESVGWDPVARMQHIDLFTWLRGDILAKADKMTMANSL
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELRVPFLDPEVFAVASRLPMQAKITRTTTKYALRRALESIVPAHVLHRPKLGFPVPIRHW
EEEEECCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LRAGELLEWAYALVNSSQAEHLVNLAAVHRMLDEHRGGISDHSRRLWTVLIFMLWHAIFI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EHSVVPQISEPQYPVQP
HHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDDRRLRTLPNEAYWIAVCGLLAFVVASARADSRGSGGTNNILQADHSMARAVHLMRHRG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
PDELSTWSDPDIDGFSGGVAFGFNRLSIIDIAHSHQPLRWGPPETPNRYILVFNGEIYNY
CCCCCCCCCCCCCCCCCCHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHH
LELRDELVIRHGAAFATDGDGESIVAGFHYWGTEILTRLRGMFAFALWDTIAHELFCARD
HHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PFGIKPLFMATGTNGTAVASEKKCLLELAEVIRFDTQIDTRALQHYTVLQYVPEPETLHR
CCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEECCCHHHHHH
GVHRLESGCYARIRPEQLDPATTRYFVPQFAATPITSSTEQTRYGEITAVLADSVAKHMR
HHHHHCCCCEEEECCCCCCCCHHHEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ADVTVGSFLSGGIDSTAIAALAIRHNPRLITFTTGFEHEGFSEIDVAAASAEAIGARHIV
HCCCHHHHHCCCCCHHHHHHEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEE
KVVTPNEFVAALPEIVWYLDEPIADPALVPLFFVAREARKHVKVVLSGEGADELFGGYRI
EEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCHHE
YREPLSLKPFDYLPGPLRRSMGKVSKSLPEGMRGKNLLHRGSLTLEERYYGNARNFSEAQ
EECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEHHHHCCCCCCCHHHH
LQAVLPRFHTGWTHTDVTAAVYAESVGWDPVARMQHIDLFTWLRGDILAKADKMTMANSL
HHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELRVPFLDPEVFAVASRLPMQAKITRTTTKYALRRALESIVPAHVLHRPKLGFPVPIRHW
EEEEECCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
LRAGELLEWAYALVNSSQAEHLVNLAAVHRMLDEHRGGISDHSRRLWTVLIFMLWHAIFI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EHSVVPQISEPQYPVQP
HHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]