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The map label for this gene is glyS [H]

Identifier: 221229841

GI number: 221229841

Start: 978884

End: 980275

Strand: Direct

Name: glyS [H]

Synonym: MLBr_00826

Alternate gene names: 221229841

Gene position: 978884-980275 (Clockwise)

Preceding gene: 221229839

Following gene: 221229842

Centisome position: 29.95

GC content: 57.18

Gene sequence:

>1392_bases
GTGCCCTGCCTCGTGGCATCCGTCATCGATACAGTAGTTAACCTGGCCAAGCGGCGCGGCTTTGTCTATCCTGCGGGTGA
GATTTACGGCGGCACAAAATCGGCGTGGGATTACGGTCCGCTCGGTGTAGAGTTCAAGGAGAACATCAAGCGGCAGTGGT
GGCGCTCGGTGGTGACCGGACGTGAGGATGTCGTGGGAATCGATTCGTCGATCATCCTGCCGCGACAAGTATGGGTGGCG
TCTGGCCACCTCGAGATCTTCCACGACCCACTGGTCGAGTCATTGATCACCCACAAGCGCTACCGTGCTGACCATCTGAT
CGAGGCCTACGAGGCTAAACATGGGCAGTCGCCGTGTAACGGCCTGGCTGATATTCGCGATCCGGACACCGGCGAGCCCG
GTCAGTGGACGGAGCCTCGGGAGTTTAACTTGATGCTGAAGACTTATCTCGGACCGATCGAGACTGAGGAGGGGTTGCAC
TATCTGCGGCCCGAGACAGCGCAGGGCATCTTCATCAACTTTGCAAACGTGGTGACGACCGCCCGCCGCAAGCCTCCGTT
CGGTATTGGACAAATTGGCAAGAGTTTTCGTAACGAGATCACCCCGGGAAACTTTATCTTCCGCACCCGCGAGTTCGAAC
AGATGGAGATGGAGTTTTTCGTGGAGCCATCGACCGCGCGGGACTGGCACCAGTACTGGATCGACACCCGGATGCAGTGG
TATGTCGAACTTGGTATCGATCCGCAGAACCTGCGGCTGTTTGAACACCCGAAGGACAAGTTGTCGCACTATTCCGACCG
TACCGTCGATATCGAATACAAGTTCGGTTTTGTCGGTAACCCGTGGGGAGAATTGGAAGGTGTTGCCAACAGAACCGACT
TCGACTTGTCAACGCACGCAAGGTATTCCGGAGTTGACCTGTCGTTCTACGATCAGGTCAACGATACTCGGTACACGCCA
TATGTCATAGAACCGGCAGCCGGTCTGACTCGATCGTTCATGGCGTTCTTGATTGACGCCTACACCGAGGATGAAGCCCC
AAATACCAGGGGCGGGATGGACAAACGTGCGCTGCTTCGACTGGATCCGCGGTTGGCACCGATTAAGGCCGCTGTGTTGC
CGCTATTCCGGCACGTCGATTTGAGCCCCAAAGCCCGTGACTTGGCCGCAGAATTGCGTAAATGCTGGAACAGCGAATTC
GACGATGCCGGCGCCATTGGGCGACGCTACCGACGCCAGGACGAGGTTGGTACGCCGTTCTGTGTGACAGTGGACTTCGA
GTCACTTGACGACAACGCCGTTACCGTGCGCGAGCGTGACGCCATGACGCAGGAGCGGGTCGCGATGACTAACGTTGCCG
ATTATCTTGCAGTGCGGCTCAAAGGTAGCTAG

Upstream 100 bases:

>100_bases
CGTGATGGCGCATGTCAAAGCTGGTGGAGGTGATCGCTACCATGACTGATGCTTTGCGCACACGGATCGTTGTTGCGCGA
CCGTTCGGATTAGAAAAGGA

Downstream 100 bases:

>100_bases
TGGAAGTGTTCTCGGGTCGTTTGAGCGTGTCGGGGCCCTACTGTCAATGATATACCGCGAAGCGCTGCAGGGTGTCAGTG
TGAACGAAGTTGTTCGCTGA

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPCLVASVIDTVVNLAKRRGFVYPAGEIYGGTKSAWDYGPLGVEFKENIKRQWWRSVVTGREDVVGIDSSIILPRQVWVA
SGHLEIFHDPLVESLITHKRYRADHLIEAYEAKHGQSPCNGLADIRDPDTGEPGQWTEPREFNLMLKTYLGPIETEEGLH
YLRPETAQGIFINFANVVTTARRKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEFFVEPSTARDWHQYWIDTRMQW
YVELGIDPQNLRLFEHPKDKLSHYSDRTVDIEYKFGFVGNPWGELEGVANRTDFDLSTHARYSGVDLSFYDQVNDTRYTP
YVIEPAAGLTRSFMAFLIDAYTEDEAPNTRGGMDKRALLRLDPRLAPIKAAVLPLFRHVDLSPKARDLAAELRKCWNSEF
DDAGAIGRRYRRQDEVGTPFCVTVDFESLDDNAVTVRERDAMTQERVAMTNVADYLAVRLKGS

Sequences:

>Translated_463_residues
MPCLVASVIDTVVNLAKRRGFVYPAGEIYGGTKSAWDYGPLGVEFKENIKRQWWRSVVTGREDVVGIDSSIILPRQVWVA
SGHLEIFHDPLVESLITHKRYRADHLIEAYEAKHGQSPCNGLADIRDPDTGEPGQWTEPREFNLMLKTYLGPIETEEGLH
YLRPETAQGIFINFANVVTTARRKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEFFVEPSTARDWHQYWIDTRMQW
YVELGIDPQNLRLFEHPKDKLSHYSDRTVDIEYKFGFVGNPWGELEGVANRTDFDLSTHARYSGVDLSFYDQVNDTRYTP
YVIEPAAGLTRSFMAFLIDAYTEDEAPNTRGGMDKRALLRLDPRLAPIKAAVLPLFRHVDLSPKARDLAAELRKCWNSEF
DDAGAIGRRYRRQDEVGTPFCVTVDFESLDDNAVTVRERDAMTQERVAMTNVADYLAVRLKGS
>Mature_462_residues
PCLVASVIDTVVNLAKRRGFVYPAGEIYGGTKSAWDYGPLGVEFKENIKRQWWRSVVTGREDVVGIDSSIILPRQVWVAS
GHLEIFHDPLVESLITHKRYRADHLIEAYEAKHGQSPCNGLADIRDPDTGEPGQWTEPREFNLMLKTYLGPIETEEGLHY
LRPETAQGIFINFANVVTTARRKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEFFVEPSTARDWHQYWIDTRMQWY
VELGIDPQNLRLFEHPKDKLSHYSDRTVDIEYKFGFVGNPWGELEGVANRTDFDLSTHARYSGVDLSFYDQVNDTRYTPY
VIEPAAGLTRSFMAFLIDAYTEDEAPNTRGGMDKRALLRLDPRLAPIKAAVLPLFRHVDLSPKARDLAAELRKCWNSEFD
DAGAIGRRYRRQDEVGTPFCVTVDFESLDDNAVTVRERDAMTQERVAMTNVADYLAVRLKGS

Specific function: Catalyzes the attachment of glycine to tRNA(Gly) [H]

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI116805340, Length=382, Percent_Identity=31.6753926701571, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI70887790, Length=440, Percent_Identity=21.8181818181818, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17554996, Length=363, Percent_Identity=29.4765840220386, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32564541, Length=363, Percent_Identity=29.4765840220386, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6325338, Length=457, Percent_Identity=26.0393873085339, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6319597, Length=396, Percent_Identity=26.5151515151515, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24664462, Length=363, Percent_Identity=31.9559228650138, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21357965, Length=363, Percent_Identity=31.9559228650138, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 52990; Mature: 52859

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCLVASVIDTVVNLAKRRGFVYPAGEIYGGTKSAWDYGPLGVEFKENIKRQWWRSVVTG
CCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
REDVVGIDSSIILPRQVWVASGHLEIFHDPLVESLITHKRYRADHLIEAYEAKHGQSPCN
CCCEEECCCCEECCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GLADIRDPDTGEPGQWTEPREFNLMLKTYLGPIETEEGLHYLRPETAQGIFINFANVVTT
CCCCCCCCCCCCCCCCCCCHHHHEEHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
ARRKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEFFVEPSTARDWHQYWIDTRMQW
HCCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHCCEEEEECCCCCHHHHHHHHHCCEEE
YVELGIDPQNLRLFEHPKDKLSHYSDRTVDIEYKFGFVGNPWGELEGVANRTDFDLSTHA
EEEECCCHHHCEEEECCHHHHHCCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCC
RYSGVDLSFYDQVNDTRYTPYVIEPAAGLTRSFMAFLIDAYTEDEAPNTRGGMDKRALLR
EECCCCEEEHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEE
LDPRLAPIKAAVLPLFRHVDLSPKARDLAAELRKCWNSEFDDAGAIGRRYRRQDEVGTPF
ECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCE
CVTVDFESLDDNAVTVRERDAMTQERVAMTNVADYLAVRLKGS
EEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEECC
>Mature Secondary Structure 
PCLVASVIDTVVNLAKRRGFVYPAGEIYGGTKSAWDYGPLGVEFKENIKRQWWRSVVTG
CHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
REDVVGIDSSIILPRQVWVASGHLEIFHDPLVESLITHKRYRADHLIEAYEAKHGQSPCN
CCCEEECCCCEECCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GLADIRDPDTGEPGQWTEPREFNLMLKTYLGPIETEEGLHYLRPETAQGIFINFANVVTT
CCCCCCCCCCCCCCCCCCCHHHHEEHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
ARRKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEFFVEPSTARDWHQYWIDTRMQW
HCCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHCCEEEEECCCCCHHHHHHHHHCCEEE
YVELGIDPQNLRLFEHPKDKLSHYSDRTVDIEYKFGFVGNPWGELEGVANRTDFDLSTHA
EEEECCCHHHCEEEECCHHHHHCCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCCCCCCC
RYSGVDLSFYDQVNDTRYTPYVIEPAAGLTRSFMAFLIDAYTEDEAPNTRGGMDKRALLR
EECCCCEEEHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEE
LDPRLAPIKAAVLPLFRHVDLSPKARDLAAELRKCWNSEFDDAGAIGRRYRRQDEVGTPF
ECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCE
CVTVDFESLDDNAVTVRERDAMTQERVAMTNVADYLAVRLKGS
EEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]