Click here to switch to the map view.

The map label for this gene is manB [H]

Identifier: 221229807

GI number: 221229807

Start: 904684

End: 906090

Strand: Direct

Name: manB [H]

Synonym: MLBr_00763

Alternate gene names: 221229807

Gene position: 904684-906090 (Clockwise)

Preceding gene: 221229806

Following gene: 221229808

Centisome position: 27.68

GC content: 60.2

Gene sequence:

>1407_bases
ATGTCTCGGCCCGCCGCGGCTGTTCACCGTGTCGTCAAGGCATATGACGTACGCGGGCTAGTGGGTAAAGAAATAGATGA
GTCGCTTAGCGCCGATTTCGGCGCGGCATTTGCGAGGTTAATGCGCGCTGAAGGTGCTCGACAGGTGGTCCTCGGCTACG
ACATGCGTGACAGTTCGCCATCGCTGGCCGCGGCTTTTGCGACAGGGGTAACTGGTCAGGGAGTTGATGTGGTGCGGATT
GGTCTATCCTCTACCGATCAGCTTTATTTCGCCGCGGGGTTGCTGGATTGCCCTGGTGCGATGTTCACCGCGAGCCACAA
TCCGGCGGCCTACAACGGCATCAAGCTGTGTCTTGCCGGCGCCAAACCGGTTGGTGCAGATACTGGGCTGGCCATCATCC
GCGACGATGTGATCGCCGGGATCCCACCGTACCCGCTTTCCCGAGGGCGGCCCGGAACCATTGCCGATCGTGATGTGCTG
GCCGACTATGGGGTGTTTTTGCGGTCGTTGGTGAACACCGCTGGGCTGCGTCCATTGCGGGTCGCCGTGGACGCCGGCAA
CGGCATGGCCGGTCACACTACACCTGCTGTGCTTGGCGCCATTGATTCGATCACCTTGTTGCCGTTGTACTTCGAGCTCG
ATGGCTCGTTTCCCAATCACGAAGCCAACCCGCTGGACCCGGCGAACCTGGTGGATCTGCAGGCCTATGTGCGTGACACC
GGCGCCGATATCGGGCTGGCCTTCGACGGGGACGCCGACCGTTGCTTCGTGGTTGACGAACGCGGTTTGTCGGTCTCACC
GTCGACGGTAACCAGCTTGGTGGCCGCTCGTGCGCTCAAACGGGAGATTGGTGCCACGATTATCTACAACGTGATCACCT
CTCGCGCGGTGCCTGAACTGGTCAGCGAGTGTGGCGGCACACCGCTGCGTTCGCGTGTTGGACATTCCTACATCAAGGCG
CTGATGGCTGAGACTGGAGCGATTTTCGGTGGTGAACATTCGGCGCACTACTACTTCCGCGACTTTTGGGGTGCCGACTC
CGGGATGCTGGCCGCGCTATATGTGCTAGCCGCTCTCGGTGAGCAGCAAAGGCCGCTCTCGGAGCTGGCCGCTGACTATC
AGCGCTACGAATCGTCCGGTGAGATCAACTTTACCGTGGCGGACGCGCCGAGCTGTATGGATGCTGTAGTGGAGTTTTTT
GGTAGTCAGATTCATTCCATCGATCACCTTGACGGGGTGACAGTGGATTTGGGCGGCGGCAGCTGGTTCAACCTGCGTAA
CTCCAACACCGAGCCGTTGCTGCGGCTCAATGTGGAGGGCCGTAGTACCGAGGATGTTGACGTGGTGGTCGCCCAGATTA
GTGCTCAAATTGCTGCCCACATATCCGGTCCGGAGGCGGAACCGTGA

Upstream 100 bases:

>100_bases
GCAGCCGGTGATTCACCGATGTCGTTACAGGCCGTTAGTGCCCGTATCTCTGTTGGGCCGATAGGCTGGCGCCAAGAGTC
CCCGTCATCAGGAGGCCCGC

Downstream 100 bases:

>100_bases
ACGCCACTCGGTTGATTGATCTCGAAGACACCAAGGGCTTGATTGCTGCTGACCGGGACGGCTTGCTGCGGGCCGCCTCG
TCGGCCGGTGCCCAAGTGCG

Product: phosphomannomutase/phosphoglucomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSRPAAAVHRVVKAYDVRGLVGKEIDESLSADFGAAFARLMRAEGARQVVLGYDMRDSSPSLAAAFATGVTGQGVDVVRI
GLSSTDQLYFAAGLLDCPGAMFTASHNPAAYNGIKLCLAGAKPVGADTGLAIIRDDVIAGIPPYPLSRGRPGTIADRDVL
ADYGVFLRSLVNTAGLRPLRVAVDAGNGMAGHTTPAVLGAIDSITLLPLYFELDGSFPNHEANPLDPANLVDLQAYVRDT
GADIGLAFDGDADRCFVVDERGLSVSPSTVTSLVAARALKREIGATIIYNVITSRAVPELVSECGGTPLRSRVGHSYIKA
LMAETGAIFGGEHSAHYYFRDFWGADSGMLAALYVLAALGEQQRPLSELAADYQRYESSGEINFTVADAPSCMDAVVEFF
GSQIHSIDHLDGVTVDLGGGSWFNLRNSNTEPLLRLNVEGRSTEDVDVVVAQISAQIAAHISGPEAEP

Sequences:

>Translated_468_residues
MSRPAAAVHRVVKAYDVRGLVGKEIDESLSADFGAAFARLMRAEGARQVVLGYDMRDSSPSLAAAFATGVTGQGVDVVRI
GLSSTDQLYFAAGLLDCPGAMFTASHNPAAYNGIKLCLAGAKPVGADTGLAIIRDDVIAGIPPYPLSRGRPGTIADRDVL
ADYGVFLRSLVNTAGLRPLRVAVDAGNGMAGHTTPAVLGAIDSITLLPLYFELDGSFPNHEANPLDPANLVDLQAYVRDT
GADIGLAFDGDADRCFVVDERGLSVSPSTVTSLVAARALKREIGATIIYNVITSRAVPELVSECGGTPLRSRVGHSYIKA
LMAETGAIFGGEHSAHYYFRDFWGADSGMLAALYVLAALGEQQRPLSELAADYQRYESSGEINFTVADAPSCMDAVVEFF
GSQIHSIDHLDGVTVDLGGGSWFNLRNSNTEPLLRLNVEGRSTEDVDVVVAQISAQIAAHISGPEAEP
>Mature_467_residues
SRPAAAVHRVVKAYDVRGLVGKEIDESLSADFGAAFARLMRAEGARQVVLGYDMRDSSPSLAAAFATGVTGQGVDVVRIG
LSSTDQLYFAAGLLDCPGAMFTASHNPAAYNGIKLCLAGAKPVGADTGLAIIRDDVIAGIPPYPLSRGRPGTIADRDVLA
DYGVFLRSLVNTAGLRPLRVAVDAGNGMAGHTTPAVLGAIDSITLLPLYFELDGSFPNHEANPLDPANLVDLQAYVRDTG
ADIGLAFDGDADRCFVVDERGLSVSPSTVTSLVAARALKREIGATIIYNVITSRAVPELVSECGGTPLRSRVGHSYIKAL
MAETGAIFGGEHSAHYYFRDFWGADSGMLAALYVLAALGEQQRPLSELAADYQRYESSGEINFTVADAPSCMDAVVEFFG
SQIHSIDHLDGVTVDLGGGSWFNLRNSNTEPLLRLNVEGRSTEDVDVVVAQISAQIAAHISGPEAEP

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=440, Percent_Identity=39.3181818181818, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1789566, Length=463, Percent_Identity=26.5658747300216, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 49316; Mature: 49185

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPAAAVHRVVKAYDVRGLVGKEIDESLSADFGAAFARLMRAEGARQVVLGYDMRDSSP
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
SLAAAFATGVTGQGVDVVRIGLSSTDQLYFAAGLLDCPGAMFTASHNPAAYNGIKLCLAG
CHHHHHHCCCCCCCCEEEEECCCCCCCEEEEECHHCCCCCEEECCCCCCCCCCEEEEEEC
AKPVGADTGLAIIRDDVIAGIPPYPLSRGRPGTIADRDVLADYGVFLRSLVNTAGLRPLR
CCCCCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
VAVDAGNGMAGHTTPAVLGAIDSITLLPLYFELDGSFPNHEANPLDPANLVDLQAYVRDT
EEEECCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GADIGLAFDGDADRCFVVDERGLSVSPSTVTSLVAARALKREIGATIIYNVITSRAVPEL
CCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSECGGTPLRSRVGHSYIKALMAETGAIFGGEHSAHYYFRDFWGADSGMLAALYVLAALG
HHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEHHHCCCCCHHHHHHHHHHHHC
EQQRPLSELAADYQRYESSGEINFTVADAPSCMDAVVEFFGSQIHSIDHLDGVTVDLGGG
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SWFNLRNSNTEPLLRLNVEGRSTEDVDVVVAQISAQIAAHISGPEAEP
CEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRPAAAVHRVVKAYDVRGLVGKEIDESLSADFGAAFARLMRAEGARQVVLGYDMRDSSP
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
SLAAAFATGVTGQGVDVVRIGLSSTDQLYFAAGLLDCPGAMFTASHNPAAYNGIKLCLAG
CHHHHHHCCCCCCCCEEEEECCCCCCCEEEEECHHCCCCCEEECCCCCCCCCCEEEEEEC
AKPVGADTGLAIIRDDVIAGIPPYPLSRGRPGTIADRDVLADYGVFLRSLVNTAGLRPLR
CCCCCCCCCCEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
VAVDAGNGMAGHTTPAVLGAIDSITLLPLYFELDGSFPNHEANPLDPANLVDLQAYVRDT
EEEECCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
GADIGLAFDGDADRCFVVDERGLSVSPSTVTSLVAARALKREIGATIIYNVITSRAVPEL
CCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSECGGTPLRSRVGHSYIKALMAETGAIFGGEHSAHYYFRDFWGADSGMLAALYVLAALG
HHHHCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEHHHCCCCCHHHHHHHHHHHHC
EQQRPLSELAADYQRYESSGEINFTVADAPSCMDAVVEFFGSQIHSIDHLDGVTVDLGGG
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
SWFNLRNSNTEPLLRLNVEGRSTEDVDVVVAQISAQIAAHISGPEAEP
CEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9673232; 10222209; 11206551; 11258796 [H]