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The map label for this gene is cofE

Identifier: 221229802

GI number: 221229802

Start: 900402

End: 901775

Strand: Reverse

Name: cofE

Synonym: MLBr_00758

Alternate gene names: 221229802

Gene position: 901775-900402 (Counterclockwise)

Preceding gene: 221229803

Following gene: 221229801

Centisome position: 27.59

GC content: 64.7

Gene sequence:

>1374_bases
GTGACCAGTTCGGATTCGCACCGCTCTGCACCGTCGCCTGAGCATGGCACCGCCTCGACGATCGAGATCCTTCCTGTCGC
CGGGCTTCCCGAATTCCGTCCCGGCGACGATCTGAGTGCCGCGCTCGCCGCGGCCGCACCGTGGCTGCGCGACGGCGACG
TCGTGGTGGTTACCAGCAAGGTGGTATCCAAGTGCGAGGGTCGGCTGGTCCCGGCTCCCGAGGATACTAGAGGACGAAAT
GAACTGCGCCGCAAGTTAATCAACGATGAAACGATACGCGTGCTGGCACGCAAAGGCAGAACCTTGATCATCGAGAACGG
GCTCGGGCTGGTGCAAGCTGCCGCCGGTGTGGACGGATCTAACGTCGGCCGGGGCGAACTCGCGCTGCTACCTGTCAATC
CCGACGCCAGCGCCGCAGTGCTGCGCATTGGGTTGCGCGCGATGCTCGGCGTCAACGTCGCGGTGGTCATCACTGATACG
ATGGGCCGCGCTTGGCGCAATGGCCAGACCGACGTCGCGATAGGTGCGGCTGGTTTGGCTGTGCTGCACAACTATTCGGG
TGCCGTCGACCGCTACGGCAACGAGTTAGTGGTCACCGAAATCGCGGTGGCCGACGAGGTCGCGGCGGCCACCGATCTGG
TCAAGGGCAAATTGACTGCGATGCCGGTGGCCGTAGTGCGCGGCCTTAGCCCGACTGACGACGGCTCGACCGCACAGCAC
CTGCTGCGAAATGGCCCCGACGACCTGTTCTGGCTCGGTACCACCGAAGCCCTCGAGCTGGGTCGCCAACAAGCCCAACT
CTTGCGCAGGTCAGTGCGCCAGTTCAGCGATGAACCAATAGCAGCGGAGCTCATCGAAACAGCCGTGGCCGAGGCCCTCA
CCGCCCCAGCCCCACACCACACCCGGCCGGTACGGTTTGTGTGGCTACAAACCCCGGCTGTCCGGACCCGGCTGCTAGAC
CGGATGGCCGACAAGTGGCGCTTAGACCTCGCCAGTGACGCCTTGCCCGCCGACGCAATAGCACAGCGTGTGGCGCGCGG
CCAGATCCTCTACGACGCACCCGAGGTCATCATTCCCTTCATGGTTCCCGACGGCGCACATGCCTACCCCGACGCCGCCC
GTGCCAGTGCCGAGCATACAATGTTCATTGTCGCTGTCGGAGCAGCGGTGCAAGCCCTGCTGGTCGCGTTGGCGGTGCGC
GGGCTGGGAAGTTGCTGGATTGGCTCAACGATCTTCGCCGACGACCTGGTCCGCGCCGAGCTCGAACTACCAGCCGACTG
GGAGCCGTTGGGCGCCATCGCGATCGGGTATGCTCACGAGCCGACCGATTTACGCGAACCGGTAAGGGTCGCCGACCTGT
TGCTACGCAAATGA

Upstream 100 bases:

>100_bases
TCGAGGGGGTCGCGGTGCGGTCGATACCGCTGCTGATGAGCAGCCCGAAGACAACGGCTGACATGGTTAACATCGGGCTT
CAGCTCGCGGGCGTGTCGGC

Downstream 100 bases:

>100_bases
ATCCTCACTGCGGCGATTAAGCTTCGCACCCGTGGCCGTCTTGCAGCCGATGCCTGGGATCAACCTGCCCAAAGATGAGC
TCACCGCTTTCGGCCGAAAA

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTSSDSHRSAPSPEHGTASTIEILPVAGLPEFRPGDDLSAALAAAAPWLRDGDVVVVTSKVVSKCEGRLVPAPEDTRGRN
ELRRKLINDETIRVLARKGRTLIIENGLGLVQAAAGVDGSNVGRGELALLPVNPDASAAVLRIGLRAMLGVNVAVVITDT
MGRAWRNGQTDVAIGAAGLAVLHNYSGAVDRYGNELVVTEIAVADEVAAATDLVKGKLTAMPVAVVRGLSPTDDGSTAQH
LLRNGPDDLFWLGTTEALELGRQQAQLLRRSVRQFSDEPIAAELIETAVAEALTAPAPHHTRPVRFVWLQTPAVRTRLLD
RMADKWRLDLASDALPADAIAQRVARGQILYDAPEVIIPFMVPDGAHAYPDAARASAEHTMFIVAVGAAVQALLVALAVR
GLGSCWIGSTIFADDLVRAELELPADWEPLGAIAIGYAHEPTDLREPVRVADLLLRK

Sequences:

>Translated_457_residues
MTSSDSHRSAPSPEHGTASTIEILPVAGLPEFRPGDDLSAALAAAAPWLRDGDVVVVTSKVVSKCEGRLVPAPEDTRGRN
ELRRKLINDETIRVLARKGRTLIIENGLGLVQAAAGVDGSNVGRGELALLPVNPDASAAVLRIGLRAMLGVNVAVVITDT
MGRAWRNGQTDVAIGAAGLAVLHNYSGAVDRYGNELVVTEIAVADEVAAATDLVKGKLTAMPVAVVRGLSPTDDGSTAQH
LLRNGPDDLFWLGTTEALELGRQQAQLLRRSVRQFSDEPIAAELIETAVAEALTAPAPHHTRPVRFVWLQTPAVRTRLLD
RMADKWRLDLASDALPADAIAQRVARGQILYDAPEVIIPFMVPDGAHAYPDAARASAEHTMFIVAVGAAVQALLVALAVR
GLGSCWIGSTIFADDLVRAELELPADWEPLGAIAIGYAHEPTDLREPVRVADLLLRK
>Mature_456_residues
TSSDSHRSAPSPEHGTASTIEILPVAGLPEFRPGDDLSAALAAAAPWLRDGDVVVVTSKVVSKCEGRLVPAPEDTRGRNE
LRRKLINDETIRVLARKGRTLIIENGLGLVQAAAGVDGSNVGRGELALLPVNPDASAAVLRIGLRAMLGVNVAVVITDTM
GRAWRNGQTDVAIGAAGLAVLHNYSGAVDRYGNELVVTEIAVADEVAAATDLVKGKLTAMPVAVVRGLSPTDDGSTAQHL
LRNGPDDLFWLGTTEALELGRQQAQLLRRSVRQFSDEPIAAELIETAVAEALTAPAPHHTRPVRFVWLQTPAVRTRLLDR
MADKWRLDLASDALPADAIAQRVARGQILYDAPEVIIPFMVPDGAHAYPDAARASAEHTMFIVAVGAAVQALLVALAVRG
LGSCWIGSTIFADDLVRAELELPADWEPLGAIAIGYAHEPTDLREPVRVADLLLRK

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the CofE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFE_MYCLE (Q9CCK2)

Other databases:

- EMBL:   AL583919
- PIR:   G87003
- RefSeq:   NP_301587.1
- ProteinModelPortal:   Q9CCK2
- SMR:   Q9CCK2
- EnsemblBacteria:   EBMYCT00000028521
- GeneID:   909714
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0758
- NMPDR:   fig|272631.1.peg.459
- Leproma:   ML0758
- GeneTree:   EBGT00050000015749
- HOGENOM:   HBG578188
- OMA:   PAPHHTT
- ProtClustDB:   PRK13294
- BioCyc:   MLEP272631:ML0758-MONOMER
- HAMAP:   MF_01259
- InterPro:   IPR002847
- InterPro:   IPR008225
- InterPro:   IPR019943
- InterPro:   IPR000415
- TIGRFAMs:   TIGR03553
- TIGRFAMs:   TIGR01916

Pfam domain/function: PF01996 F420_ligase; PF00881 Nitroreductase; SSF55469 Nitroreductase

EC number: NA

Molecular weight: Translated: 48562; Mature: 48431

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSDSHRSAPSPEHGTASTIEILPVAGLPEFRPGDDLSAALAAAAPWLRDGDVVVVTSK
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHH
VVSKCEGRLVPAPEDTRGRNELRRKLINDETIRVLARKGRTLIIENGLGLVQAAAGVDGS
HHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCC
NVGRGELALLPVNPDASAAVLRIGLRAMLGVNVAVVITDTMGRAWRNGQTDVAIGAAGLA
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHCCCCCCEEEHHHHHH
VLHNYSGAVDRYGNELVVTEIAVADEVAAATDLVKGKLTAMPVAVVRGLSPTDDGSTAQH
HHHCCCCHHHHCCCEEEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHH
LLRNGPDDLFWLGTTEALELGRQQAQLLRRSVRQFSDEPIAAELIETAVAEALTAPAPHH
HHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
TRPVRFVWLQTPAVRTRLLDRMADKWRLDLASDALPADAIAQRVARGQILYDAPEVIIPF
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCEEEEE
MVPDGAHAYPDAARASAEHTMFIVAVGAAVQALLVALAVRGLGSCWIGSTIFADDLVRAE
ECCCCCCCCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHH
LELPADWEPLGAIAIGYAHEPTDLREPVRVADLLLRK
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSDSHRSAPSPEHGTASTIEILPVAGLPEFRPGDDLSAALAAAAPWLRDGDVVVVTSK
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHH
VVSKCEGRLVPAPEDTRGRNELRRKLINDETIRVLARKGRTLIIENGLGLVQAAAGVDGS
HHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCC
NVGRGELALLPVNPDASAAVLRIGLRAMLGVNVAVVITDTMGRAWRNGQTDVAIGAAGLA
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHHCCCCCCEEEHHHHHH
VLHNYSGAVDRYGNELVVTEIAVADEVAAATDLVKGKLTAMPVAVVRGLSPTDDGSTAQH
HHHCCCCHHHHCCCEEEEEEEHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHHH
LLRNGPDDLFWLGTTEALELGRQQAQLLRRSVRQFSDEPIAAELIETAVAEALTAPAPHH
HHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
TRPVRFVWLQTPAVRTRLLDRMADKWRLDLASDALPADAIAQRVARGQILYDAPEVIIPF
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCEEEEE
MVPDGAHAYPDAARASAEHTMFIVAVGAAVQALLVALAVRGLGSCWIGSTIFADDLVRAE
ECCCCCCCCCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHH
LELPADWEPLGAIAIGYAHEPTDLREPVRVADLLLRK
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002