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The map label for this gene is rmlA2 [H]

Identifier: 221229798

GI number: 221229798

Start: 895486

End: 896562

Strand: Direct

Name: rmlA2 [H]

Synonym: MLBr_00753

Alternate gene names: 221229798

Gene position: 895486-896562 (Clockwise)

Preceding gene: 221229797

Following gene: 221229804

Centisome position: 27.4

GC content: 61.1

Gene sequence:

>1077_bases
TTGGCAACCTTGCAAGTCGATGCGGTGGTCCTGGTCGGCGGCAAGGGCACCCGGCTGCGGCCGCTGACCTTGTCTGCGCC
CAAGCCCATGCTGCCCACCGCCGGGTTGCCGTTTCTTACTCATCTGCTGTCGCGGATCGCTGCGGCGGGCATTGAGCACG
TGATTCTGAGTACCTCATATCGGGACGCAGTGTTTGAGGCGGAGTTCGGCGATGGGTCCAAGCTGGGCCTGCAAATCGAC
TACGTGATTGAGGAGTCTCCGCTGGGAACTGGAGGTGGTATCGCCAACGTCATCGATCAGCTGCGTCACGACACCGTCAT
GGTGTTCAACGGCGATGTGTTGTCCGGTGTTGACCTCGGGCAGTTGCTGGGATTCCAACGAACCAACTTCGCTGACGTCA
CTTTGCACCTGGTGCGAGTGGGTGACCCACGGGCTTTCGGTTGTGTGTCCACCGAAGATGGTCGAGTTACCGCCTTTCTG
GAGAAAACGCAAGATCCGCCTACCGACCAGATTAACGCCGGCTGTTACGTCTTCGAGCGCAATGTTATCGACCGGATTCC
TCGAGGCCGTGAGGTATCGGTTGAACGCGAGGTGTTCCCGACCCTACTCTCCGACGCCGACGTTAAGGTCTGTGGTTACG
TTGATGCCACGTATTGGCGGGACATGGGTACCCCAGAAGACTTTGTTCGCGGGTCGGCGGATCTGGTGCGCGGCATTGCC
CCATCACCGGCCTTGGGCGGCCACCGCGGCGAGCACTTAGTGCATGACGGTGCGGCGGTGTCTCCTGGTGCGGTGCTGAT
CGGCGGCACGGTTGTTGGGCGTGGTGCTGAGATCGGCCCCGGTGTCCGGTTAGACGGAGCGGTGATTTTTGACGGGGCCA
AGGTTGAGGCCGGCAGTGTGATCGAGCGCTCGATCCTCGGCTTCGGTGTCCGCATTGGGCCGCGGGCACTGATACGCGAC
GGTGTAATTGGAGACGGCGCTGATATCGGTGCGCGTTGTGAGTTGTTGCGTGGTGCTCGAGTGTGGCCCGGTGTTTCGAT
TCCCGACGGCGGGATTCGTTACTCCTCCGATGTCTAG

Upstream 100 bases:

>100_bases
CGTGGCTCGTTGGTGGTGCGTTCTCGCCTGATGGTGCGTAGTTCGCGTCGTAAACAGGCTGCGAAGCAGGCCAGGCAACT
GGCAGAAGGGCGACGTTGAG

Downstream 100 bases:

>100_bases
CTTCTTGGGCATCGAAATCGACGCTACCGGGATGACTACTCGAACTTTTCCGCGCTGACGTCGATCTCCGGTGCTCATGT
CGGTGGGCTAGACGACGATC

Product: putative sugar-phosphate nucleotidyl transferase

Products: NA

Alternate protein names: ATP-mannose-1-phosphate guanylyltransferase; GDP-mannose pyrophosphorylase; NDP-hexose pyrophosphorylase [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MATLQVDAVVLVGGKGTRLRPLTLSAPKPMLPTAGLPFLTHLLSRIAAAGIEHVILSTSYRDAVFEAEFGDGSKLGLQID
YVIEESPLGTGGGIANVIDQLRHDTVMVFNGDVLSGVDLGQLLGFQRTNFADVTLHLVRVGDPRAFGCVSTEDGRVTAFL
EKTQDPPTDQINAGCYVFERNVIDRIPRGREVSVEREVFPTLLSDADVKVCGYVDATYWRDMGTPEDFVRGSADLVRGIA
PSPALGGHRGEHLVHDGAAVSPGAVLIGGTVVGRGAEIGPGVRLDGAVIFDGAKVEAGSVIERSILGFGVRIGPRALIRD
GVIGDGADIGARCELLRGARVWPGVSIPDGGIRYSSDV

Sequences:

>Translated_358_residues
MATLQVDAVVLVGGKGTRLRPLTLSAPKPMLPTAGLPFLTHLLSRIAAAGIEHVILSTSYRDAVFEAEFGDGSKLGLQID
YVIEESPLGTGGGIANVIDQLRHDTVMVFNGDVLSGVDLGQLLGFQRTNFADVTLHLVRVGDPRAFGCVSTEDGRVTAFL
EKTQDPPTDQINAGCYVFERNVIDRIPRGREVSVEREVFPTLLSDADVKVCGYVDATYWRDMGTPEDFVRGSADLVRGIA
PSPALGGHRGEHLVHDGAAVSPGAVLIGGTVVGRGAEIGPGVRLDGAVIFDGAKVEAGSVIERSILGFGVRIGPRALIRD
GVIGDGADIGARCELLRGARVWPGVSIPDGGIRYSSDV
>Mature_357_residues
ATLQVDAVVLVGGKGTRLRPLTLSAPKPMLPTAGLPFLTHLLSRIAAAGIEHVILSTSYRDAVFEAEFGDGSKLGLQIDY
VIEESPLGTGGGIANVIDQLRHDTVMVFNGDVLSGVDLGQLLGFQRTNFADVTLHLVRVGDPRAFGCVSTEDGRVTAFLE
KTQDPPTDQINAGCYVFERNVIDRIPRGREVSVEREVFPTLLSDADVKVCGYVDATYWRDMGTPEDFVRGSADLVRGIAP
SPALGGHRGEHLVHDGAAVSPGAVLIGGTVVGRGAEIGPGVRLDGAVIFDGAKVEAGSVIERSILGFGVRIGPRALIRDG
VIGDGADIGARCELLRGARVWPGVSIPDGGIRYSSDV

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=361, Percent_Identity=33.7950138504155, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI11761619, Length=349, Percent_Identity=32.9512893982808, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI31881779, Length=399, Percent_Identity=27.0676691729323, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI45447090, Length=399, Percent_Identity=27.0676691729323, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788351, Length=234, Percent_Identity=26.9230769230769, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790224, Length=184, Percent_Identity=27.7173913043478, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI133931050, Length=359, Percent_Identity=31.7548746518106, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17509979, Length=337, Percent_Identity=26.1127596439169, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509981, Length=324, Percent_Identity=24.6913580246914, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320148, Length=354, Percent_Identity=29.0960451977401, Blast_Score=153, Evalue=5e-38,
Organism=Drosophila melanogaster, GI21355443, Length=352, Percent_Identity=33.5227272727273, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24644084, Length=352, Percent_Identity=33.5227272727273, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24653912, Length=372, Percent_Identity=27.9569892473118, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 37713; Mature: 37582

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLQVDAVVLVGGKGTRLRPLTLSAPKPMLPTAGLPFLTHLLSRIAAAGIEHVILSTSY
CCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHEEEEECCC
RDAVFEAEFGDGSKLGLQIDYVIEESPLGTGGGIANVIDQLRHDTVMVFNGDVLSGVDLG
CCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCEECCCCHH
QLLGFQRTNFADVTLHLVRVGDPRAFGCVSTEDGRVTAFLEKTQDPPTDQINAGCYVFER
HHHCCCCCCCEEEEEEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEEEEC
NVIDRIPRGREVSVEREVFPTLLSDADVKVCGYVDATYWRDMGTPEDFVRGSADLVRGIA
HHHHHCCCCCCEEEHHHHHHHHHCCCCEEEEEEECHHHHHCCCCHHHHHCCCHHHHHCCC
PSPALGGHRGEHLVHDGAAVSPGAVLIGGTVVGRGAEIGPGVRLDGAVIFDGAKVEAGSV
CCCCCCCCCCCEEEECCCCCCCCEEEEECEEEECCCCCCCCEEECCEEEECCCEECCCHH
IERSILGFGVRIGPRALIRDGVIGDGADIGARCELLRGARVWPGVSIPDGGIRYSSDV
HHHHHHHCCEEECCHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCCC
>Mature Secondary Structure 
ATLQVDAVVLVGGKGTRLRPLTLSAPKPMLPTAGLPFLTHLLSRIAAAGIEHVILSTSY
CEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHEEEEECCC
RDAVFEAEFGDGSKLGLQIDYVIEESPLGTGGGIANVIDQLRHDTVMVFNGDVLSGVDLG
CCEEEEEECCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCEECCCCHH
QLLGFQRTNFADVTLHLVRVGDPRAFGCVSTEDGRVTAFLEKTQDPPTDQINAGCYVFER
HHHCCCCCCCEEEEEEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCEEEEEC
NVIDRIPRGREVSVEREVFPTLLSDADVKVCGYVDATYWRDMGTPEDFVRGSADLVRGIA
HHHHHCCCCCCEEEHHHHHHHHHCCCCEEEEEEECHHHHHCCCCHHHHHCCCHHHHHCCC
PSPALGGHRGEHLVHDGAAVSPGAVLIGGTVVGRGAEIGPGVRLDGAVIFDGAKVEAGSV
CCCCCCCCCCCEEEECCCCCCCCEEEEECEEEECCCCCCCCEEECCEEEECCCEECCCHH
IERSILGFGVRIGPRALIRDGVIGDGADIGARCELLRGARVWPGVSIPDGGIRYSSDV
HHHHHHHCCEEECCHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8334170 [H]