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The map label for this gene is birA [H]

Identifier: 221229787

GI number: 221229787

Start: 873729

End: 874553

Strand: Direct

Name: birA [H]

Synonym: MLBr_00732

Alternate gene names: 221229787

Gene position: 873729-874553 (Clockwise)

Preceding gene: 221229780

Following gene: 221229788

Centisome position: 26.74

GC content: 63.52

Gene sequence:

>825_bases
TTGGGTAGTGATCTACTCAAGCCGCCGCTGGATCCAGCGGCACTGCGTGCTGAGCTGATTGGGACGGGGCTCCGGTGGCG
TCAACTGGACGTCGTCACGCAAACCGGTTCCACGAACGCTGACCTGTTGGCGCGGGCAGCGTCAGGCATCGACATCGACG
GAGCGGTGCTTATTGCCGAGCACCAGACCGCTGGCCGCGGGCGTCACGGCCGCGGCTGGTCGGCTAGCCCTCGCACACAA
ATCACGATGTCGATCGGTGTGAGTATCGCTGACGTTCCGGTTGCCACGTGGGCCTGGTTGTCGCTGGCCACTGGGGTAGC
GGTGCTGGACACGGTGGCCCCGTTGATTGACGTGACCGGCGTTGACGCCGGCCTCAAGTGGCCCAACGATGTTCTGGCCG
GTCCACCAGGCTCCAAAGGTAAACTCGCGGGCATCCTGGCTGAGGTTGTGCGACCAGTCATCGTCGTCGGTGTGGGACTT
AACGTCACGCAGGCCCCAAAAGAGGTCGACGGTCCTGGGGCTACCTCGTTGCTTGACCTTGGCGTGCCGGCACCCGATCG
GGAGCAGCTGGTGTGCTGGCTGTTGCGAGAACTCGATGCGCGGGTTGGCCAGTGGCGAAGCGCCGACCCACAGTTGATGG
CCGATTATCGGGCGCGTAGCCCGATAATCGGGTCACGTGTGCGTGCGGAGCTCCCCGGCGGCAAAGACGTGGTCGGAACC
GTGCGCGGCATCGATGACCAGGGAAGGTTGTGCTTGAAAACAGAAGGGCCTACCAAAGGACAAACCGTCGTGATTTCGGC
TGGTGACGTGGTGCATTTGCGCTAG

Upstream 100 bases:

>100_bases
TCCATTGAATGAGCCGAGTGGTCGGTAACGCTTGTCATGGGAGTCGATGTTATCGGCAGCTGCTGTGGTGGTGGTTACGC
TGGGTTTGCGAGGAGATGAG

Downstream 100 bases:

>100_bases
TTTCATCCGGGGCGCCGCTTGCGTTGGGATAGGCGTTTTGCAGGTATTCTCTATAGATTTTCTTTGCAAAACGTCAATCT
CGACGATTCCACCTCAGACG

Product: biotin apo-protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MGSDLLKPPLDPAALRAELIGTGLRWRQLDVVTQTGSTNADLLARAASGIDIDGAVLIAEHQTAGRGRHGRGWSASPRTQ
ITMSIGVSIADVPVATWAWLSLATGVAVLDTVAPLIDVTGVDAGLKWPNDVLAGPPGSKGKLAGILAEVVRPVIVVGVGL
NVTQAPKEVDGPGATSLLDLGVPAPDREQLVCWLLRELDARVGQWRSADPQLMADYRARSPIIGSRVRAELPGGKDVVGT
VRGIDDQGRLCLKTEGPTKGQTVVISAGDVVHLR

Sequences:

>Translated_274_residues
MGSDLLKPPLDPAALRAELIGTGLRWRQLDVVTQTGSTNADLLARAASGIDIDGAVLIAEHQTAGRGRHGRGWSASPRTQ
ITMSIGVSIADVPVATWAWLSLATGVAVLDTVAPLIDVTGVDAGLKWPNDVLAGPPGSKGKLAGILAEVVRPVIVVGVGL
NVTQAPKEVDGPGATSLLDLGVPAPDREQLVCWLLRELDARVGQWRSADPQLMADYRARSPIIGSRVRAELPGGKDVVGT
VRGIDDQGRLCLKTEGPTKGQTVVISAGDVVHLR
>Mature_273_residues
GSDLLKPPLDPAALRAELIGTGLRWRQLDVVTQTGSTNADLLARAASGIDIDGAVLIAEHQTAGRGRHGRGWSASPRTQI
TMSIGVSIADVPVATWAWLSLATGVAVLDTVAPLIDVTGVDAGLKWPNDVLAGPPGSKGKLAGILAEVVRPVIVVGVGLN
VTQAPKEVDGPGATSLLDLGVPAPDREQLVCWLLRELDARVGQWRSADPQLMADYRARSPIIGSRVRAELPGGKDVVGTV
RGIDDQGRLCLKTEGPTKGQTVVISAGDVVHLR

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=234, Percent_Identity=32.0512820512821, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28585; Mature: 28454

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDLLKPPLDPAALRAELIGTGLRWRQLDVVTQTGSTNADLLARAASGIDIDGAVLIAE
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEE
HQTAGRGRHGRGWSASPRTQITMSIGVSIADVPVATWAWLSLATGVAVLDTVAPLIDVTG
CCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDAGLKWPNDVLAGPPGSKGKLAGILAEVVRPVIVVGVGLNVTQAPKEVDGPGATSLLDL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEC
GVPAPDREQLVCWLLRELDARVGQWRSADPQLMADYRARSPIIGSRVRAELPGGKDVVGT
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCEEEH
VRGIDDQGRLCLKTEGPTKGQTVVISAGDVVHLR
EECCCCCCCEEEEECCCCCCCEEEEECCCEEEEC
>Mature Secondary Structure 
GSDLLKPPLDPAALRAELIGTGLRWRQLDVVTQTGSTNADLLARAASGIDIDGAVLIAE
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEE
HQTAGRGRHGRGWSASPRTQITMSIGVSIADVPVATWAWLSLATGVAVLDTVAPLIDVTG
CCCCCCCCCCCCCCCCCCEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDAGLKWPNDVLAGPPGSKGKLAGILAEVVRPVIVVGVGLNVTQAPKEVDGPGATSLLDL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEC
GVPAPDREQLVCWLLRELDARVGQWRSADPQLMADYRARSPIIGSRVRAELPGGKDVVGT
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCCCEEEH
VRGIDDQGRLCLKTEGPTKGQTVVISAGDVVHLR
EECCCCCCCEEEEECCCCCCCEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]