Click here to switch to the map view.

The map label for this gene is deoD

Identifier: 221229777

GI number: 221229777

Start: 845469

End: 846275

Strand: Reverse

Name: deoD

Synonym: MLBr_00707

Alternate gene names: 221229777

Gene position: 846275-845469 (Counterclockwise)

Preceding gene: 221229778

Following gene: 221229776

Centisome position: 25.9

GC content: 64.31

Gene sequence:

>807_bases
GTGACTTACACCCTGCTCGATCCCGACGAACTCGCTCGGCGGGCCGCCCAGGTTATTGGTGAGCGCACCGGTATCCTTAA
GCACGACGTCGCAGTCGTCCTCGGATCGGGATGGTCCTCGGCGGTTGCAGCGCTCGGCTCATCGAGAGCCGTGTTCCCCC
AGGCCGAGCTGCCCGGGTTCATAACGCCCAACGCAGCCGGGCATACCGGCGAGTTGTTGTCGGTGCGTATTGGCGCGCAT
CGGGTGTTGGTGCTGGCCGGTCGCATCCATCCCTACGAGGGGCATGACCTTAGGCACGTCGTCCATCCAGTACGCACGGC
GTGCGCGGCCGGTGCACGCATCATCGTTCTCACTAATGCGGCCGGCGGACTGCGTGCAGACATGGCGGTCGGCCAACTGG
TGCTGATTAGTGACCACCTGAACCTGACGACACGTTCGCCGCTAGTCGGCACGCACTTCGTCGACTTAACCAACGCGTAC
ACAACGCGGCTCCGAAAACTCGCCAGCGACACCGACCCGACACTGACCGAAGGCGTGTACGCGGCCCAGCCCGGCCCACA
CTATGAGACTCCCGCGGAAATCCGGATGCTGCGGATGCTGGGTGCTGACCTAGTGGGCATGTCAACGGTGCACGAGACCA
TCGCAGCACGGGCTGCGGGCGCTGAGGTGTTGGGCGTGTCACTGGTGACAAACCTGGCGGCCGGGATCACCGGCAAGCCA
CTTAACCATGCTGAGGTGCTTGCCGCGGGGACTGCGTCAGCGAACCGGATCGGGTCCCTGCTGGCCGACATCATAGCCCG
GTTTTGA

Upstream 100 bases:

>100_bases
CAGTATCGCACCGACCTGCAGCTGCTCTGACCACAATGATGGCTCACTCGTCACTCACGCCGTTCCGGCACTCATCGTCG
CTGGGCTAAGCTCGCCGACT

Downstream 100 bases:

>100_bases
AGGCTGCCGTGACACCCGAAGAGTGGATCACGCACGATCCCGACCCACAGACAGCCGCCGAGCTGGCCGCGTGCGACCCC
GATGAACTTGCGGCGCGGTT

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: PNP; Inosine phosphorylase

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTYTLLDPDELARRAAQVIGERTGILKHDVAVVLGSGWSSAVAALGSSRAVFPQAELPGFITPNAAGHTGELLSVRIGAH
RVLVLAGRIHPYEGHDLRHVVHPVRTACAAGARIIVLTNAAGGLRADMAVGQLVLISDHLNLTTRSPLVGTHFVDLTNAY
TTRLRKLASDTDPTLTEGVYAAQPGPHYETPAEIRMLRMLGADLVGMSTVHETIAARAAGAEVLGVSLVTNLAAGITGKP
LNHAEVLAAGTASANRIGSLLADIIARF

Sequences:

>Translated_268_residues
MTYTLLDPDELARRAAQVIGERTGILKHDVAVVLGSGWSSAVAALGSSRAVFPQAELPGFITPNAAGHTGELLSVRIGAH
RVLVLAGRIHPYEGHDLRHVVHPVRTACAAGARIIVLTNAAGGLRADMAVGQLVLISDHLNLTTRSPLVGTHFVDLTNAY
TTRLRKLASDTDPTLTEGVYAAQPGPHYETPAEIRMLRMLGADLVGMSTVHETIAARAAGAEVLGVSLVTNLAAGITGKP
LNHAEVLAAGTASANRIGSLLADIIARF
>Mature_267_residues
TYTLLDPDELARRAAQVIGERTGILKHDVAVVLGSGWSSAVAALGSSRAVFPQAELPGFITPNAAGHTGELLSVRIGAHR
VLVLAGRIHPYEGHDLRHVVHPVRTACAAGARIIVLTNAAGGLRADMAVGQLVLISDHLNLTTRSPLVGTHFVDLTNAYT
TRLRKLASDTDPTLTEGVYAAQPGPHYETPAEIRMLRMLGADLVGMSTVHETIAARAAGAEVLGVSLVTNLAAGITGKPL
NHAEVLAAGTASANRIGSLLADIIARF

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family

Homologues:

Organism=Homo sapiens, GI157168362, Length=260, Percent_Identity=36.9230769230769, Blast_Score=147, Evalue=8e-36,
Organism=Escherichia coli, GI1788746, Length=249, Percent_Identity=36.5461847389558, Blast_Score=156, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17541190, Length=282, Percent_Identity=36.8794326241135, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323238, Length=270, Percent_Identity=34.4444444444444, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI45552887, Length=282, Percent_Identity=32.2695035460993, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45552885, Length=282, Percent_Identity=32.2695035460993, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24656090, Length=278, Percent_Identity=32.0143884892086, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24656093, Length=278, Percent_Identity=32.0143884892086, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24762376, Length=222, Percent_Identity=29.2792792792793, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUNA_MYCLE (P46862)

Other databases:

- EMBL:   U00022
- EMBL:   AL583919
- PIR:   S73042
- RefSeq:   NP_301562.1
- ProteinModelPortal:   P46862
- SMR:   P46862
- EnsemblBacteria:   EBMYCT00000027913
- GeneID:   910380
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0707
- NMPDR:   fig|272631.1.peg.434
- Leproma:   ML0707
- GeneTree:   EBGT00050000015947
- HOGENOM:   HBG292914
- OMA:   VLISDHI
- ProtClustDB:   PRK08202
- BioCyc:   MLEP272631:ML0707-MONOMER
- BRENDA:   2.4.2.1
- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011269
- InterPro:   IPR001369
- InterPro:   IPR018099
- PANTHER:   PTHR11904
- PANTHER:   PTHR11904:SF9
- TIGRFAMs:   TIGR01697
- TIGRFAMs:   TIGR01698

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =2.4.2.1

Molecular weight: Translated: 27981; Mature: 27849

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYTLLDPDELARRAAQVIGERTGILKHDVAVVLGSGWSSAVAALGSSRAVFPQAELPGF
CEEEECCHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCC
ITPNAAGHTGELLSVRIGAHRVLVLAGRIHPYEGHDLRHVVHPVRTACAAGARIIVLTNA
CCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
AGGLRADMAVGQLVLISDHLNLTTRSPLVGTHFVDLTNAYTTRLRKLASDTDPTLTEGVY
CCCCCHHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCEE
AAQPGPHYETPAEIRMLRMLGADLVGMSTVHETIAARAAGAEVLGVSLVTNLAAGITGKP
ECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
LNHAEVLAAGTASANRIGSLLADIIARF
CCHHHHEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYTLLDPDELARRAAQVIGERTGILKHDVAVVLGSGWSSAVAALGSSRAVFPQAELPGF
EEEECCHHHHHHHHHHHHHHHCCCHHHCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCC
ITPNAAGHTGELLSVRIGAHRVLVLAGRIHPYEGHDLRHVVHPVRTACAAGARIIVLTNA
CCCCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECC
AGGLRADMAVGQLVLISDHLNLTTRSPLVGTHFVDLTNAYTTRLRKLASDTDPTLTEGVY
CCCCCHHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCEE
AAQPGPHYETPAEIRMLRMLGADLVGMSTVHETIAARAAGAEVLGVSLVTNLAAGITGKP
ECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
LNHAEVLAAGTASANRIGSLLADIIARF
CCHHHHEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002