Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 221229769

GI number: 221229769

Start: 827886

End: 829121

Strand: Reverse

Name: dacB [H]

Synonym: MLBr_00691

Alternate gene names: 221229769

Gene position: 829121-827886 (Counterclockwise)

Preceding gene: 221229770

Following gene: 221229766

Centisome position: 25.37

GC content: 62.7

Gene sequence:

>1236_bases
ATGTCTTTTTTACGTTCCACCGCATGCCTGGCAGCAGCAGTTTTCGCGATCGTTGGACCAGTCGCGCTGGACCTGCCTAC
CGCCGTGCCAGTCGGACATGCCGAACCGAATGCCGGACCCAACGCCGGTCCAGCAACATGCCCATACCAGGTGTCCACTC
CACCAGCGGTGGACTCGTCGGAAGTCCCCCAAACCGGTAACCCACCGATGCCGCTGGCAGTGCCGCCCAAACCGGTCGGC
GGCGCGGCACTGAACAGCTGCGGAATCGTCACCGCTCCAGACACGCCGCCAGTACCAGGTGACCTCTCCGCCGAGGCGTG
GCTGGTGGCGGATCTGAACAGCGGCGCCGTCATCGCTGCCCGCGATCCACACGGCCGACACCGCCCGGCCAGCATCATTA
AAGTGCTGTTAGCCATGGCGTCCATCAACACGCTCAACCTTAACAAATCGGTCGCCGGAACTGCCGAGGACGCCGCCGCC
GAGGGCACCAAGGTCGGCGTCCACGACGGCGGCACATACACTATCAACCAACTACTGCACGGGTTGTTGATGCAGTCGGG
CAATGACGCCGCGCACTCGTTGGCCATGCAGCTCGGCGGTATGCAGCAAGCGCTGCAGAAGATCAACGTGCTGGCCGCCA
AACTCGGCGGCCAGGATACTCGGGTAGCAACGCCATCGGGGCTGGACGGGCCGGGCATGAGCACGTCGGCCTACGACATC
GGCTTGTTCTACCGGTACGCCTGGCAGAACCCCTCGTTCAGCAACATCGTCGCTACCCGGACCTTCAATTTCCCCGGTCC
CGGCGACCATCCCGGCTACCAGCTGGAAAACGACAACCAGTTGCTCTACCAATATCCGGGTGCGTTGGGCGGCAAGACCG
GCTACACCGACGACGCTGGCCAAACGTTTGTGGGCGCCGCCAACCGTAATGGTCGACGGCTGATGGCGGTGCTGCTACAC
GGGACCCGGCAGCCGATTGCGCCGTGGGAACAAGCAGCGCACTTGTTGGACTATGGCTTTAGCACCGCAGTCGGAACCCA
GATAGGCACACTGATCGAACCCGACCCCACGGTGGCCGGTAACGCAGATCGGCACGGCCATGGCCTACAAGCCGCCGGAA
TCATGTCATCAAACGACACGCTTCCGGTACGAATAGGCGTAGCGGTCGTCGGTACGTTGATCGTGTTCGGCTTAATCATG
ATCACGCGTTCGCTAAACCGCCGGCCACAACACTGA

Upstream 100 bases:

>100_bases
CGCCTGCCAAGGTGAGTTAGCACACTTTGCAACTTCTGTACCATCAATCACAGCGTGGCTTACGCTAACTAGACGCCCGA
TCGCCTACTCTGCACACACG

Downstream 100 bases:

>100_bases
ACAGGCCTGAACAATCTGGATTTCATTCACAACATTTTGGTTGTGGTGGCGTCTCTGCCGTGATGAAGACCCTTAACCAG
GACGTTTCCCTGGTTCTCGA

Product: putative D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSFLRSTACLAAAVFAIVGPVALDLPTAVPVGHAEPNAGPNAGPATCPYQVSTPPAVDSSEVPQTGNPPMPLAVPPKPVG
GAALNSCGIVTAPDTPPVPGDLSAEAWLVADLNSGAVIAARDPHGRHRPASIIKVLLAMASINTLNLNKSVAGTAEDAAA
EGTKVGVHDGGTYTINQLLHGLLMQSGNDAAHSLAMQLGGMQQALQKINVLAAKLGGQDTRVATPSGLDGPGMSTSAYDI
GLFYRYAWQNPSFSNIVATRTFNFPGPGDHPGYQLENDNQLLYQYPGALGGKTGYTDDAGQTFVGAANRNGRRLMAVLLH
GTRQPIAPWEQAAHLLDYGFSTAVGTQIGTLIEPDPTVAGNADRHGHGLQAAGIMSSNDTLPVRIGVAVVGTLIVFGLIM
ITRSLNRRPQH

Sequences:

>Translated_411_residues
MSFLRSTACLAAAVFAIVGPVALDLPTAVPVGHAEPNAGPNAGPATCPYQVSTPPAVDSSEVPQTGNPPMPLAVPPKPVG
GAALNSCGIVTAPDTPPVPGDLSAEAWLVADLNSGAVIAARDPHGRHRPASIIKVLLAMASINTLNLNKSVAGTAEDAAA
EGTKVGVHDGGTYTINQLLHGLLMQSGNDAAHSLAMQLGGMQQALQKINVLAAKLGGQDTRVATPSGLDGPGMSTSAYDI
GLFYRYAWQNPSFSNIVATRTFNFPGPGDHPGYQLENDNQLLYQYPGALGGKTGYTDDAGQTFVGAANRNGRRLMAVLLH
GTRQPIAPWEQAAHLLDYGFSTAVGTQIGTLIEPDPTVAGNADRHGHGLQAAGIMSSNDTLPVRIGVAVVGTLIVFGLIM
ITRSLNRRPQH
>Mature_410_residues
SFLRSTACLAAAVFAIVGPVALDLPTAVPVGHAEPNAGPNAGPATCPYQVSTPPAVDSSEVPQTGNPPMPLAVPPKPVGG
AALNSCGIVTAPDTPPVPGDLSAEAWLVADLNSGAVIAARDPHGRHRPASIIKVLLAMASINTLNLNKSVAGTAEDAAAE
GTKVGVHDGGTYTINQLLHGLLMQSGNDAAHSLAMQLGGMQQALQKINVLAAKLGGQDTRVATPSGLDGPGMSTSAYDIG
LFYRYAWQNPSFSNIVATRTFNFPGPGDHPGYQLENDNQLLYQYPGALGGKTGYTDDAGQTFVGAANRNGRRLMAVLLHG
TRQPIAPWEQAAHLLDYGFSTAVGTQIGTLIEPDPTVAGNADRHGHGLQAAGIMSSNDTLPVRIGVAVVGTLIVFGLIMI
TRSLNRRPQH

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=266, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786851, Length=291, Percent_Identity=29.2096219931272, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787062, Length=281, Percent_Identity=28.4697508896797, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42452; Mature: 42320

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLRSTACLAAAVFAIVGPVALDLPTAVPVGHAEPNAGPNAGPATCPYQVSTPPAVDSS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCC
EVPQTGNPPMPLAVPPKPVGGAALNSCGIVTAPDTPPVPGDLSAEAWLVADLNSGAVIAA
CCCCCCCCCCCCCCCCCCCCCCHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCEEEEE
RDPHGRHRPASIIKVLLAMASINTLNLNKSVAGTAEDAAAEGTKVGVHDGGTYTINQLLH
CCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHCCCCEEEECCCCCEEHHHHHH
GLLMQSGNDAAHSLAMQLGGMQQALQKINVLAAKLGGQDTRVATPSGLDGPGMSTSAYDI
HHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEE
GLFYRYAWQNPSFSNIVATRTFNFPGPGDHPGYQLENDNQLLYQYPGALGGKTGYTDDAG
EEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCC
QTFVGAANRNGRRLMAVLLHGTRQPIAPWEQAAHLLDYGFSTAVGTQIGTLIEPDPTVAG
CEEEECCCCCCCEEEEEEHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCEECCCCCCCC
NADRHGHGLQAAGIMSSNDTLPVRIGVAVVGTLIVFGLIMITRSLNRRPQH
CCCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SFLRSTACLAAAVFAIVGPVALDLPTAVPVGHAEPNAGPNAGPATCPYQVSTPPAVDSS
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCC
EVPQTGNPPMPLAVPPKPVGGAALNSCGIVTAPDTPPVPGDLSAEAWLVADLNSGAVIAA
CCCCCCCCCCCCCCCCCCCCCCHHCCCCEEECCCCCCCCCCCCCCEEEEEECCCCEEEEE
RDPHGRHRPASIIKVLLAMASINTLNLNKSVAGTAEDAAAEGTKVGVHDGGTYTINQLLH
CCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCHHHHCCCCEEEECCCCCEEHHHHHH
GLLMQSGNDAAHSLAMQLGGMQQALQKINVLAAKLGGQDTRVATPSGLDGPGMSTSAYDI
HHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEE
GLFYRYAWQNPSFSNIVATRTFNFPGPGDHPGYQLENDNQLLYQYPGALGGKTGYTDDAG
EEEEEEECCCCCCCCEEEEEEECCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCC
QTFVGAANRNGRRLMAVLLHGTRQPIAPWEQAAHLLDYGFSTAVGTQIGTLIEPDPTVAG
CEEEECCCCCCCEEEEEEHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCEECCCCCCCC
NADRHGHGLQAAGIMSSNDTLPVRIGVAVVGTLIVFGLIMITRSLNRRPQH
CCCCCCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]