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The map label for this gene is rsmE [H]

Identifier: 221229734

GI number: 221229734

Start: 759050

End: 759823

Strand: Direct

Name: rsmE [H]

Synonym: MLBr_00626

Alternate gene names: 221229734

Gene position: 759050-759823 (Clockwise)

Preceding gene: 221229733

Following gene: 221229735

Centisome position: 23.23

GC content: 65.89

Gene sequence:

>774_bases
GTGGTGAGCACATTGTTCTACGTCGACGCATTGCCCGATACCGGTACGGTAGCTGTGGTGGGTGGCGACGAAGGGTTTCA
CGCGGTCACGGTGCGTCGGATCCGTCCCGGCGAGGAGCTGGTGCTCGGTGACGGTGTCGGTGGCCTTGCTCACTGCTTGG
TCGAACGGGCCGGGCGCGATGGGCTATATGCCCGGGTGCTGGGGCACTGGACCGTAGCGCCGGGGCTACCGCCGGCGACT
GTGGTGCAGGCGTTGCCCAAGGCTGAACGCTCAGAGTTGGCTATCGAAATGGCCACCGAGGCCGGTGCCGATGCGTTCGT
GGCGTGGCAGTCTGATCGTTGCGTGGCCAGCTGGTACGGGGTCCGGGTGGACAAGGGTCTGCGCCGGTGGCGTGCGGTAG
CCCGCGCGGCAGCTCGGCAGTCTCGCCGGGCGCACATACCTTCGGTCGATGGGGTGTTGACCACTGCGGCGCTGCTACAG
CGGATCCGGGATGAGGTAGCCGCCGGTGCCACTGTGCTGGCTTTGCACGAGTCGGCGACCGATCGGTTGTCCGATGTCTT
TGCGGATATCTCTGTGGCGCAAGCAAACTCGCTAGTGTTGTTGGTCGGTCCCGAAGGAGGTATCGCGCCGGACGAGATTG
CCGCATTGACTGAGGCGGGCGCCGTCGCGGTCCGGCTCGGTCCCACCGTGCTGCGGTCATCGACCGCGGCTGCGGTGGCA
TTGGGCGCGTTAGGCGTGCTCACTTCCCGGTGGGATGCTTCATCGATTCTGTAA

Upstream 100 bases:

>100_bases
GTTCGTTCGACGCATGCGGCCGGCGGCGGACTGTTCAGCCGGCTGCGCGAGACCTTTACCAATCGTTAGCACGGTTTTAA
CGGTTTTAGGGGCTCCACAT

Downstream 100 bases:

>100_bases
TCCAAGTCACGTTTCTACCGCGTCGTTACAGTCTCACGAAGTACGAATAGGGGGCTCAAGCGATGACCAGCAGCTGCGAT
CAGGGGTGTTTGCTGGTGCA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MVSTLFYVDALPDTGTVAVVGGDEGFHAVTVRRIRPGEELVLGDGVGGLAHCLVERAGRDGLYARVLGHWTVAPGLPPAT
VVQALPKAERSELAIEMATEAGADAFVAWQSDRCVASWYGVRVDKGLRRWRAVARAAARQSRRAHIPSVDGVLTTAALLQ
RIRDEVAAGATVLALHESATDRLSDVFADISVAQANSLVLLVGPEGGIAPDEIAALTEAGAVAVRLGPTVLRSSTAAAVA
LGALGVLTSRWDASSIL

Sequences:

>Translated_257_residues
MVSTLFYVDALPDTGTVAVVGGDEGFHAVTVRRIRPGEELVLGDGVGGLAHCLVERAGRDGLYARVLGHWTVAPGLPPAT
VVQALPKAERSELAIEMATEAGADAFVAWQSDRCVASWYGVRVDKGLRRWRAVARAAARQSRRAHIPSVDGVLTTAALLQ
RIRDEVAAGATVLALHESATDRLSDVFADISVAQANSLVLLVGPEGGIAPDEIAALTEAGAVAVRLGPTVLRSSTAAAVA
LGALGVLTSRWDASSIL
>Mature_257_residues
MVSTLFYVDALPDTGTVAVVGGDEGFHAVTVRRIRPGEELVLGDGVGGLAHCLVERAGRDGLYARVLGHWTVAPGLPPAT
VVQALPKAERSELAIEMATEAGADAFVAWQSDRCVASWYGVRVDKGLRRWRAVARAAARQSRRAHIPSVDGVLTTAALLQ
RIRDEVAAGATVLALHESATDRLSDVFADISVAQANSLVLLVGPEGGIAPDEIAALTEAGAVAVRLGPTVLRSSTAAAVA
LGALGVLTSRWDASSIL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=246, Percent_Identity=26.4227642276423, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26699; Mature: 26699

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSTLFYVDALPDTGTVAVVGGDEGFHAVTVRRIRPGEELVLGDGVGGLAHCLVERAGRD
CCCEEEEEECCCCCCEEEEEECCCCEEEEEEEEECCCCEEEEECCCHHHHHHHHHHCCCC
GLYARVLGHWTVAPGLPPATVVQALPKAERSELAIEMATEAGADAFVAWQSDRCVASWYG
CCEEHHEECEEECCCCCHHHHHHHCCCCCHHHHEEEEHHHCCCCEEEEECCCCEEEHHHC
VRVDKGLRRWRAVARAAARQSRRAHIPSVDGVLTTAALLQRIRDEVAAGATVLALHESAT
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHH
DRLSDVFADISVAQANSLVLLVGPEGGIAPDEIAALTEAGAVAVRLGPTVLRSSTAAAVA
HHHHHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECHHHHHCCHHHHHH
LGALGVLTSRWDASSIL
HHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MVSTLFYVDALPDTGTVAVVGGDEGFHAVTVRRIRPGEELVLGDGVGGLAHCLVERAGRD
CCCEEEEEECCCCCCEEEEEECCCCEEEEEEEEECCCCEEEEECCCHHHHHHHHHHCCCC
GLYARVLGHWTVAPGLPPATVVQALPKAERSELAIEMATEAGADAFVAWQSDRCVASWYG
CCEEHHEECEEECCCCCHHHHHHHCCCCCHHHHEEEEHHHCCCCEEEEECCCCEEEHHHC
VRVDKGLRRWRAVARAAARQSRRAHIPSVDGVLTTAALLQRIRDEVAAGATVLALHESAT
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHH
DRLSDVFADISVAQANSLVLLVGPEGGIAPDEIAALTEAGAVAVRLGPTVLRSSTAAAVA
HHHHHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECHHHHHCCHHHHHH
LGALGVLTSRWDASSIL
HHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]