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The map label for this gene is ribH

Identifier: 221229689

GI number: 221229689

Start: 677615

End: 678097

Strand: Direct

Name: ribH

Synonym: MLBr_00560

Alternate gene names: 221229689

Gene position: 677615-678097 (Clockwise)

Preceding gene: 221229688

Following gene: 221229690

Centisome position: 20.73

GC content: 60.04

Gene sequence:

>483_bases
ATGAGTGGTGGAGCCGGTATTCCGGAGGTTCCGGGGATCGACGCTTCCGGTCTGCGGCTAGGTATTGTCGCAAGCACCTG
GCACAGCAGAATTTGCGATGCGCTGTTGGCGGGCGCGCGCAAAGTTGCTGCCGACTCGGGTATCGATGGCCCGACAGTAG
TCCGTGTGCTCGGTGCCATCGAAATTCCGGTAGTGGTGCAGGAGCTCGCTCGCCACCACGATGCAGTTGTTGCCCTGGGT
GTCGTGATCCGTGGCGATACACCACATTTCGATTATGTTTGCAATTCCGTAACGCAAGGCTTGACGCGCATAGCGCTAGA
TACCTCGACGCCGGTTGGCAACGGTGTGCTGACCACCAATACCGAGAAACAGGCGCTGGATCGGGCTGGGCTTCCGACGT
CGGCCGAAGACAAGGGGGCCCAGGCGGCAGCTGCTGCGCTGACCACCGCATTGACCCTGCTTAACTTGCGTTCTCGGATA
TGA

Upstream 100 bases:

>100_bases
GTTACTTGATGACCAAGCGTGACAAGATGGGTCACGACCTGGGCGGGTTGGATGATTTTCATGAATCAAACTATTTTCCA
GGTGAATTCGGCGGTGCTTT

Downstream 100 bases:

>100_bases
CCGCACAGTTGGATCGGGACGATTGGGATGTGGAATTGCGTCCCTACTGGACGCCGTTATTTGCCTACGCTGCAGCGTTT
CTGATCGCGGCAGCGCATAT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MSGGAGIPEVPGIDASGLRLGIVASTWHSRICDALLAGARKVAADSGIDGPTVVRVLGAIEIPVVVQELARHHDAVVALG
VVIRGDTPHFDYVCNSVTQGLTRIALDTSTPVGNGVLTTNTEKQALDRAGLPTSAEDKGAQAAAAALTTALTLLNLRSRI

Sequences:

>Translated_160_residues
MSGGAGIPEVPGIDASGLRLGIVASTWHSRICDALLAGARKVAADSGIDGPTVVRVLGAIEIPVVVQELARHHDAVVALG
VVIRGDTPHFDYVCNSVTQGLTRIALDTSTPVGNGVLTTNTEKQALDRAGLPTSAEDKGAQAAAAALTTALTLLNLRSRI
>Mature_159_residues
SGGAGIPEVPGIDASGLRLGIVASTWHSRICDALLAGARKVAADSGIDGPTVVRVLGAIEIPVVVQELARHHDAVVALGV
VIRGDTPHFDYVCNSVTQGLTRIALDTSTPVGNGVLTTNTEKQALDRAGLPTSAEDKGAQAAAAALTTALTLLNLRSRI

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=147, Percent_Identity=42.1768707482993, Blast_Score=93, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6324429, Length=151, Percent_Identity=33.7748344370861, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_MYCLB (B8ZUN3)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503105.1
- ProteinModelPortal:   B8ZUN3
- SMR:   B8ZUN3
- EnsemblBacteria:   EBMYCT00000086412
- GeneID:   7326620
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016935
- HOGENOM:   HBG311126
- OMA:   SSRALMD
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16337; Mature: 16206

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGAGIPEVPGIDASGLRLGIVASTWHSRICDALLAGARKVAADSGIDGPTVVRVLGAI
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EIPVVVQELARHHDAVVALGVVIRGDTPHFDYVCNSVTQGLTRIALDTSTPVGNGVLTTN
HHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECC
TEKQALDRAGLPTSAEDKGAQAAAAALTTALTLLNLRSRI
CHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGGAGIPEVPGIDASGLRLGIVASTWHSRICDALLAGARKVAADSGIDGPTVVRVLGAI
CCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EIPVVVQELARHHDAVVALGVVIRGDTPHFDYVCNSVTQGLTRIALDTSTPVGNGVLTTN
HHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECC
TEKQALDRAGLPTSAEDKGAQAAAAALTTALTLLNLRSRI
CHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA