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The map label for this gene is gmk

Identifier: 221229675

GI number: 221229675

Start: 657275

End: 657907

Strand: Direct

Name: gmk

Synonym: MLBr_00541

Alternate gene names: 221229675

Gene position: 657275-657907 (Clockwise)

Preceding gene: 221229674

Following gene: 221229676

Centisome position: 20.11

GC content: 63.35

Gene sequence:

>633_bases
GTGCCAGTGAGCGCGCGCGGGGCACCGGATGCTGAGCACTGGGCATGGAGTGAGCAAACGGATAAAGGACGTGTGGTTGT
GCTGTCCGGTCCCTCGGCGGTCGGCAAGTCAACGGTGGTCAGGTGTCTGCGCGAACGGGTGTCGAACCTGCATTTCAGTG
TGTCGGCCACGACGCGAGAGCCTAGGCCAGATGAGATGGACGGTGTCGATTATCACTTCGTCAGCCCCGCCCGATTCCAA
CAGCTGATCGACCAGGGTGCATTGCTGGAATGGGCTGAAATCCACGGCGGGATGCACCGTTCGGGCACCCTGGCCGAGCC
GGTGCGGGTCGCCGCTGCGGCCGGTTTTCCGGTGCTTATCGAGGTTGACCTGGCCGGAGCCAGGGCGGTCAAGAAGGCGA
TGCCTGAGGCCATCGCCGTGTTTTTAGCGCCGCCCAGCTGGGAGGATTTGGAAGCCAGGCTGGTTGGCCGCGGCACCGAA
ACACCCGAGGCCATTCGGCGCCGGCTCGAGACCGCGCGCATCGAATTGGCGGCACAAGATGACTTCGATGAAGTTGTGGT
CAACCGTCGATTGGAGTCTGCGTGTGCCGAATTGGTATCCTTGCTGGTGGGAGCCGTGTCGGGCTCGGCATGA

Upstream 100 bases:

>100_bases
GCGAGCGTCAGCGCAAAGCGCTACTGGAAAAGTTCGGCTCCGCTTAGCTAGCCCAGCCGACGATGCAGGCTGCTTGATCT
ATGGAGGCACCGGCGCAGGA

Downstream 100 bases:

>100_bases
GCCATCCAGCCAACCGGACCCGAGCACCGAATTCCTTAACTGCATTCATCACCGTTCTATCCCGCCAGGAGATTGTCTTA
CGTGAGTATTCCGCAGTCCA

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MPVSARGAPDAEHWAWSEQTDKGRVVVLSGPSAVGKSTVVRCLRERVSNLHFSVSATTREPRPDEMDGVDYHFVSPARFQ
QLIDQGALLEWAEIHGGMHRSGTLAEPVRVAAAAGFPVLIEVDLAGARAVKKAMPEAIAVFLAPPSWEDLEARLVGRGTE
TPEAIRRRLETARIELAAQDDFDEVVVNRRLESACAELVSLLVGAVSGSA

Sequences:

>Translated_210_residues
MPVSARGAPDAEHWAWSEQTDKGRVVVLSGPSAVGKSTVVRCLRERVSNLHFSVSATTREPRPDEMDGVDYHFVSPARFQ
QLIDQGALLEWAEIHGGMHRSGTLAEPVRVAAAAGFPVLIEVDLAGARAVKKAMPEAIAVFLAPPSWEDLEARLVGRGTE
TPEAIRRRLETARIELAAQDDFDEVVVNRRLESACAELVSLLVGAVSGSA
>Mature_209_residues
PVSARGAPDAEHWAWSEQTDKGRVVVLSGPSAVGKSTVVRCLRERVSNLHFSVSATTREPRPDEMDGVDYHFVSPARFQQ
LIDQGALLEWAEIHGGMHRSGTLAEPVRVAAAAGFPVLIEVDLAGARAVKKAMPEAIAVFLAPPSWEDLEARLVGRGTET
PEAIRRRLETARIELAAQDDFDEVVVNRRLESACAELVSLLVGAVSGSA

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874833, Length=179, Percent_Identity=40.7821229050279, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI4504221, Length=179, Percent_Identity=40.7821229050279, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI226874831, Length=179, Percent_Identity=40.7821229050279, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI38570142, Length=194, Percent_Identity=29.8969072164948, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI14780902, Length=219, Percent_Identity=28.310502283105, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1790080, Length=183, Percent_Identity=33.879781420765, Blast_Score=123, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI193203132, Length=184, Percent_Identity=37.5, Blast_Score=116, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17508835, Length=184, Percent_Identity=37.5, Blast_Score=116, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6320662, Length=184, Percent_Identity=41.8478260869565, Blast_Score=139, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21356141, Length=184, Percent_Identity=34.7826086956522, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_MYCLE (Q9CCQ7)

Other databases:

- EMBL:   AL583918
- PIR:   E86976
- RefSeq:   NP_301460.1
- ProteinModelPortal:   Q9CCQ7
- SMR:   Q9CCQ7
- EnsemblBacteria:   EBMYCT00000028833
- GeneID:   909307
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0541
- NMPDR:   fig|272631.1.peg.332
- Leproma:   ML0541
- GeneTree:   EBGT00050000017746
- HOGENOM:   HBG671982
- OMA:   EWAEFAG
- ProtClustDB:   PRK00300
- BioCyc:   MLEP272631:ML0541-MONOMER
- BRENDA:   2.7.4.8
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 22658; Mature: 22527

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSARGAPDAEHWAWSEQTDKGRVVVLSGPSAVGKSTVVRCLRERVSNLHFSVSATTRE
CCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEECCCC
PRPDEMDGVDYHFVSPARFQQLIDQGALLEWAEIHGGMHRSGTLAEPVRVAAAAGFPVLI
CCCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEE
EVDLAGARAVKKAMPEAIAVFLAPPSWEDLEARLVGRGTETPEAIRRRLETARIELAAQD
EEECCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHEEEECCC
DFDEVVVNRRLESACAELVSLLVGAVSGSA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PVSARGAPDAEHWAWSEQTDKGRVVVLSGPSAVGKSTVVRCLRERVSNLHFSVSATTRE
CCCCCCCCCHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEECCCC
PRPDEMDGVDYHFVSPARFQQLIDQGALLEWAEIHGGMHRSGTLAEPVRVAAAAGFPVLI
CCCCCCCCCCEEEECHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEE
EVDLAGARAVKKAMPEAIAVFLAPPSWEDLEARLVGRGTETPEAIRRRLETARIELAAQD
EEECCCHHHHHHHHHHHEEEEECCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHEEEECCC
DFDEVVVNRRLESACAELVSLLVGAVSGSA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002