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The map label for this gene is nusB

Identifier: 221229663

GI number: 221229663

Start: 633542

End: 634114

Strand: Direct

Name: nusB

Synonym: MLBr_00523

Alternate gene names: 221229663

Gene position: 633542-634114 (Clockwise)

Preceding gene: 221229662

Following gene: 221229664

Centisome position: 19.39

GC content: 60.21

Gene sequence:

>573_bases
ATGTCTAATCCGGTCAAGGGACGCCATCAGGCCCGTAAGCGTGCCGTCGACCTGTTGTTCGAAGCCGAGGCCCGCGATCT
GAGCCCACTCGAGATAATCGAGGTTCGTAGCGCATTGGCGAAATCCAAGCTGGATGTAGCGCCGCTGCATCCGTATACGG
TCGTGGTAGCCCAAGGTGTTAGCGAGCACACCGCCCGTATTGACGAACTGATAATCTCGCATCTACAAGGCTGGAAGCTC
GATCGGCTGCCAGCAGTGGATCGCGCCATTTTGCGAGTCTCGATATGGGAGCTGCTATACGCTGACGATGTGCCCGAACC
GGTCGCTGTCGACGAGGCGGTCGAGCTGGCCAAGGAGCTGTCTACTGACGACTCACCTGGCTTCGTCAACGGATTGCTGG
GCAAAGTTATGCTGGTTACGCCGCAGATCCGTGCGGCCGCTCAAGCAGTCCAGCAGGCGGTGCGCATGGCTGCCGGTACT
TCCGAGGATCACGTACCGCAGCGTGAGCCGGCTGCTGGCCAATTGGGTCAGGACGATTCGAACGGCGGCCAAGTCGCTGC
TGTCTGCCGGTGA

Upstream 100 bases:

>100_bases
CCGGCGCTCAGATAAATGTTCCGCTGTTCATTAATACCGGCGACAAGCTCAAGGTGGATTCACGCGACGGCAGCTACCTG
GGACGTGTGAACGTCTGATC

Downstream 100 bases:

>100_bases
TGGTCGCGATCATGGGTTTGACGCAGGGTGTGCTAAGTTCGCCCAGCGATGTCACTGGCCTGTTTGCGCGCCGTTGGGAG
CTTCCAGTTCCGCGCTGGAT

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSNPVKGRHQARKRAVDLLFEAEARDLSPLEIIEVRSALAKSKLDVAPLHPYTVVVAQGVSEHTARIDELIISHLQGWKL
DRLPAVDRAILRVSIWELLYADDVPEPVAVDEAVELAKELSTDDSPGFVNGLLGKVMLVTPQIRAAAQAVQQAVRMAAGT
SEDHVPQREPAAGQLGQDDSNGGQVAAVCR

Sequences:

>Translated_190_residues
MSNPVKGRHQARKRAVDLLFEAEARDLSPLEIIEVRSALAKSKLDVAPLHPYTVVVAQGVSEHTARIDELIISHLQGWKL
DRLPAVDRAILRVSIWELLYADDVPEPVAVDEAVELAKELSTDDSPGFVNGLLGKVMLVTPQIRAAAQAVQQAVRMAAGT
SEDHVPQREPAAGQLGQDDSNGGQVAAVCR
>Mature_189_residues
SNPVKGRHQARKRAVDLLFEAEARDLSPLEIIEVRSALAKSKLDVAPLHPYTVVVAQGVSEHTARIDELIISHLQGWKLD
RLPAVDRAILRVSIWELLYADDVPEPVAVDEAVELAKELSTDDSPGFVNGLLGKVMLVTPQIRAAAQAVQQAVRMAAGTS
EDHVPQREPAAGQLGQDDSNGGQVAAVCR

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=86, Percent_Identity=43.0232558139535, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_MYCLB (B8ZUK7)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002503079.1
- ProteinModelPortal:   B8ZUK7
- SMR:   B8ZUK7
- EnsemblBacteria:   EBMYCT00000085777
- GeneID:   7325221
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017513
- HOGENOM:   HBG734409
- OMA:   DDSPSFV
- ProtClustDB:   PRK00202
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 20520; Mature: 20388

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPVKGRHQARKRAVDLLFEAEARDLSPLEIIEVRSALAKSKLDVAPLHPYTVVVAQGV
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCH
SEHTARIDELIISHLQGWKLDRLPAVDRAILRVSIWELLYADDVPEPVAVDEAVELAKEL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
STDDSPGFVNGLLGKVMLVTPQIRAAAQAVQQAVRMAAGTSEDHVPQREPAAGQLGQDDS
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
NGGQVAAVCR
CCCEEEEEEC
>Mature Secondary Structure 
SNPVKGRHQARKRAVDLLFEAEARDLSPLEIIEVRSALAKSKLDVAPLHPYTVVVAQGV
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCH
SEHTARIDELIISHLQGWKLDRLPAVDRAILRVSIWELLYADDVPEPVAVDEAVELAKEL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
STDDSPGFVNGLLGKVMLVTPQIRAAAQAVQQAVRMAAGTSEDHVPQREPAAGQLGQDDS
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
NGGQVAAVCR
CCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA