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The map label for this gene is ruvC

Identifier: 221229636

GI number: 221229636

Start: 581233

End: 581799

Strand: Direct

Name: ruvC

Synonym: MLBr_00481

Alternate gene names: 221229636

Gene position: 581233-581799 (Clockwise)

Preceding gene: 221229635

Following gene: 221229637

Centisome position: 17.79

GC content: 61.55

Gene sequence:

>567_bases
ATGCGCGTGATGGGCGTCGATCCCGGGTTGACGCGGTGCGGGTTGTCTGTCGTCGAAAATGGGCGCGGTAGCCAAGTTGT
TGCGTTGGATGTCGATGTGGTGCGCACACCATCGGACGCACCGGTATCCAAGCGCTTGCTGGCTGTCAGCGATGTCGTCG
AACACTGGCTGGACGCCCATCATCCGGATGTTATGGCCATCGAACGGGTTTTTTCTCAGCAGAATGTGTCGACGGTGATG
GGTACGGCACAAGCCGGCGGTGTGATCGCGTTGGCCGCGGCGAGACGCGGTATCGACGTACATTTCCATACCCCAAGTGA
GGTTAAGGCCGCAGTTACCGGCAATGGTGCCGCCAACAAAGCGCAAGTTACCGCGATGGTGACCAGAATCCTTGCACTGC
AAGCTAAACCGACACCGGCCGATGCGGCCGACGCGTTGGCCCTGGCGATCTGCCACTGCTGGAGAGCACCAATGATCGCG
CGGATGGCAGAAGCCGAAGCGCTGGGCGCGCGGCACCGTCAGGCCTATCGGGCCAAGGTAGCTGGCGAGGTTAAGGCCAC
CCGGTGA

Upstream 100 bases:

>100_bases
GGCCGGCCGACAAGCCATGCTACCGCGTGTCCTACCAGATGCTGTCTAGACTGGCCTCTAGGCTATCGAACATTGTGCGC
TGTTAGATGGGGAGTATGTG

Downstream 100 bases:

>100_bases
TTTTCTCGGTACGCGGCGAGGTACTCGAGGTGGCACTTGACCACGCCGTCATCGAAGCCGCCGGTATCGGCTATCGGGTA
AACGCGACGCCGTCGGCGTT

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MRVMGVDPGLTRCGLSVVENGRGSQVVALDVDVVRTPSDAPVSKRLLAVSDVVEHWLDAHHPDVMAIERVFSQQNVSTVM
GTAQAGGVIALAAARRGIDVHFHTPSEVKAAVTGNGAANKAQVTAMVTRILALQAKPTPADAADALALAICHCWRAPMIA
RMAEAEALGARHRQAYRAKVAGEVKATR

Sequences:

>Translated_188_residues
MRVMGVDPGLTRCGLSVVENGRGSQVVALDVDVVRTPSDAPVSKRLLAVSDVVEHWLDAHHPDVMAIERVFSQQNVSTVM
GTAQAGGVIALAAARRGIDVHFHTPSEVKAAVTGNGAANKAQVTAMVTRILALQAKPTPADAADALALAICHCWRAPMIA
RMAEAEALGARHRQAYRAKVAGEVKATR
>Mature_188_residues
MRVMGVDPGLTRCGLSVVENGRGSQVVALDVDVVRTPSDAPVSKRLLAVSDVVEHWLDAHHPDVMAIERVFSQQNVSTVM
GTAQAGGVIALAAARRGIDVHFHTPSEVKAAVTGNGAANKAQVTAMVTRILALQAKPTPADAADALALAICHCWRAPMIA
RMAEAEALGARHRQAYRAKVAGEVKATR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=171, Percent_Identity=36.8421052631579, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_MYCLE (P40834)

Other databases:

- EMBL:   U00011
- EMBL:   AL583918
- PIR:   S72733
- RefSeq:   NP_301421.1
- ProteinModelPortal:   P40834
- SMR:   P40834
- EnsemblBacteria:   EBMYCT00000028413
- GeneID:   909230
- GenomeReviews:   AL450380_GR
- KEGG:   mle:ML0481
- NMPDR:   fig|272631.1.peg.293
- Leproma:   ML0481
- GeneTree:   EBGT00050000016429
- HOGENOM:   HBG437552
- OMA:   RIAYQMT
- ProtClustDB:   PRK00039
- BioCyc:   MLEP272631:ML0481-MONOMER
- BRENDA:   3.1.22.4
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 19785; Mature: 19785

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS01321 RUVC; PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVMGVDPGLTRCGLSVVENGRGSQVVALDVDVVRTPSDAPVSKRLLAVSDVVEHWLDAH
CEEECCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
HPDVMAIERVFSQQNVSTVMGTAQAGGVIALAAARRGIDVHFHTPSEVKAAVTGNGAANK
CCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCCCCEEEEECCHHHEEEEECCCCCCH
AQVTAMVTRILALQAKPTPADAADALALAICHCWRAPMIARMAEAEALGARHRQAYRAKV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AGEVKATR
HCCCCCCC
>Mature Secondary Structure
MRVMGVDPGLTRCGLSVVENGRGSQVVALDVDVVRTPSDAPVSKRLLAVSDVVEHWLDAH
CEEECCCCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
HPDVMAIERVFSQQNVSTVMGTAQAGGVIALAAARRGIDVHFHTPSEVKAAVTGNGAANK
CCCHHHHHHHHHHCCHHHHHHCCCCCCEEEEEECCCCCEEEEECCHHHEEEEECCCCCCH
AQVTAMVTRILALQAKPTPADAADALALAICHCWRAPMIARMAEAEALGARHRQAYRAKV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AGEVKATR
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002