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The map label for this gene is pdxT [H]

Identifier: 221229634

GI number: 221229634

Start: 575478

End: 576149

Strand: Direct

Name: pdxT [H]

Synonym: MLBr_00474

Alternate gene names: 221229634

Gene position: 575478-576149 (Clockwise)

Preceding gene: 221229633

Following gene: 221229635

Centisome position: 17.61

GC content: 63.69

Gene sequence:

>672_bases
GTGGCAGCCGTCATGCAGGAGGGGCTGATCCGTTTTCCCGCGGGATACCCGGCTGACTCCGCAGTGGAGCGTGTTGTGAG
CTTTCCGAGGGTGGGCGTCTTGGCGCTGCAGGGCGATACCCGTGAGCATCTCACCGCGCTGCGCGAAGCCGGGGCCGACT
CCATGCCGGTACGTCGCCGCGGTGAGCTGGACGAGGTTGACGCGCTGGTCATTCCCGGCGGCGAATCCACTACCATCAGC
CACCTGCTGCTCGACTGCGAGTTGCTAGAGCCGTTGCGTGCGCGTCTGGCCGACGGGTTGCCGGCTTACGGTGCGTGCAC
GGGTATGATTCTACTGGCCAGTGAGATCCTCGACGCTGGCGTGTGTGGGCGTGAGGCGCTGCCGTTGGGTGCTATCGATA
TTACAGTGCGGCGCAACGCTTTTGGACGCCAAGTCGATTCATTTGAAGGCGATATTGGGTTCGCTGGTCTTGTTGATCCG
GTGCGTGCGGTGTTCATCCGTGCGCCCTGGGTGGAACGGGCCGGCGATGGCGTGCAGGTGCTGGCTCAAGCTGCGGGCCA
CGCTGTCGCGGTGCGGCAGGGCTCAATGTTGGCTACGGCGTTTCACCCGGAGATGACCAGCGACCGGCGCATCCACCAGT
TATTCGTTGACATTGTCAACGGAATCGCCTGA

Upstream 100 bases:

>100_bases
TGGTGGATGCATTGCTAAATGAGCAGCTACCTGAACTGTTTGAGTCGTAACAGGTTCCAAATGCCTCTTTTTCGCTTGCT
TATGCCACAACGCGGCTATG

Downstream 100 bases:

>100_bases
GTTTCAGGAAAGTGCACATCAGTCACATCTGCACCATCAACAGGTGACGGGGTGATTTCGAAAAATTCATAGCGCATGCG
TTATCACTCCTTACAAACCG

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MAAVMQEGLIRFPAGYPADSAVERVVSFPRVGVLALQGDTREHLTALREAGADSMPVRRRGELDEVDALVIPGGESTTIS
HLLLDCELLEPLRARLADGLPAYGACTGMILLASEILDAGVCGREALPLGAIDITVRRNAFGRQVDSFEGDIGFAGLVDP
VRAVFIRAPWVERAGDGVQVLAQAAGHAVAVRQGSMLATAFHPEMTSDRRIHQLFVDIVNGIA

Sequences:

>Translated_223_residues
MAAVMQEGLIRFPAGYPADSAVERVVSFPRVGVLALQGDTREHLTALREAGADSMPVRRRGELDEVDALVIPGGESTTIS
HLLLDCELLEPLRARLADGLPAYGACTGMILLASEILDAGVCGREALPLGAIDITVRRNAFGRQVDSFEGDIGFAGLVDP
VRAVFIRAPWVERAGDGVQVLAQAAGHAVAVRQGSMLATAFHPEMTSDRRIHQLFVDIVNGIA
>Mature_222_residues
AAVMQEGLIRFPAGYPADSAVERVVSFPRVGVLALQGDTREHLTALREAGADSMPVRRRGELDEVDALVIPGGESTTISH
LLLDCELLEPLRARLADGLPAYGACTGMILLASEILDAGVCGREALPLGAIDITVRRNAFGRQVDSFEGDIGFAGLVDPV
RAVFIRAPWVERAGDGVQVLAQAAGHAVAVRQGSMLATAFHPEMTSDRRIHQLFVDIVNGIA

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS [H]

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=211, Percent_Identity=34.1232227488152, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323995, Length=211, Percent_Identity=31.2796208530806, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321048, Length=211, Percent_Identity=30.8056872037915, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002161
- InterPro:   IPR021196 [H]

Pfam domain/function: PF01174 SNO [H]

EC number: NA

Molecular weight: Translated: 23705; Mature: 23573

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS51130 PDXT_SNO_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVMQEGLIRFPAGYPADSAVERVVSFPRVGVLALQGDTREHLTALREAGADSMPVRRR
CCCHHHCCCEECCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCC
GELDEVDALVIPGGESTTISHLLLDCELLEPLRARLADGLPAYGACTGMILLASEILDAG
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
VCGREALPLGAIDITVRRNAFGRQVDSFEGDIGFAGLVDPVRAVFIRAPWVERAGDGVQV
CCCCCCCCCCEEEEEEECCHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHH
LAQAAGHAVAVRQGSMLATAFHPEMTSDRRIHQLFVDIVNGIA
HHHHCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAVMQEGLIRFPAGYPADSAVERVVSFPRVGVLALQGDTREHLTALREAGADSMPVRRR
CCHHHCCCEECCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCC
GELDEVDALVIPGGESTTISHLLLDCELLEPLRARLADGLPAYGACTGMILLASEILDAG
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
VCGREALPLGAIDITVRRNAFGRQVDSFEGDIGFAGLVDPVRAVFIRAPWVERAGDGVQV
CCCCCCCCCCEEEEEEECCHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHH
LAQAAGHAVAVRQGSMLATAFHPEMTSDRRIHQLFVDIVNGIA
HHHHCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]