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The map label for this gene is ilvX [H]

Identifier: 221229573

GI number: 221229573

Start: 447423

End: 448970

Strand: Direct

Name: ilvX [H]

Synonym: MLBr_00354

Alternate gene names: 221229573

Gene position: 447423-448970 (Clockwise)

Preceding gene: 221229572

Following gene: 221229574

Centisome position: 13.69

GC content: 64.79

Gene sequence:

>1548_bases
GTGAACGGTGCTCAGGCCATGATCAGTACCCTGGTCGACGGTGGTGTCGATGTGTGCTTCGCCAATCCCGGCACCTCGGA
GATGCACTTCGTGGCCGCACTGGACACCGTTGCCGGAATGCGTGGCGTGCTAACCCTTTTCGAAGGTGTGGCCACTGGTG
CGGCCGACGGCTACGCTCGTATAGCTGGCCGGCCGGCAGTGGTGCTGCTGCACCTGGGTCCCGGATTAGGGAACGGCCTG
GCTAACCTGCACAACGCGCGCCGCGCACGGGTGCCCATGGTGGTGGTCGTCGGCGACCATGCCACCTATCACAAGAAGTA
TGATGCCCCACTGGAATCCGACATCGACGCTCTGGCAGGAACCGTTTCAGGATGGCTGCACCGGACAATGGACACCGCCG
ACGTTGGGGTCGACGCAGCGGAGGCGATCTCTGCCAGCCTGGTGGGCTCGCAGATCTCGACGCTGATCCTGCCCGCGGAC
GTATCCTGGTCTGACGGCGCCATTCCCGCCGCGTCGGTGTCAGACCAGCCAGCCAGTGCCGGTGTCGACGTGGGGGCGGT
TCGCACGGCCCTGGAATCGGGAGAACCCACGGTGATTTTGATCGGTGCAGACGCCACCGGCTGGTCGGGGTTGGCAGCGG
TCGCGCGGATCGGTGCGGCGACCGGCGCCCGCTGGTTCTGCGAGACGTTCCCGGCCCGATTGGAGCGGGGCGCTGGTGTC
CCTGCTGTCGAACGACTCGCCTACTTTGCCGAGGTCGCCGCGGCTCAGCTGGACGGTGCCAAGCATCTAGTGCTGGCCGG
TGCCCAGTCGCCGGTGTCGTTCTTTGCCTACCCTGGCATGCCTAGTGACTTGGTACCAGCCGGCTGTGAGGTGCACGTGC
TTGCCGAGCACGGCGGTGCCGCTTACGCGCTAAACGCATTGGCTGACGAGGTGGCATCCGGGACCGCTGCGCCGGTGGTG
GCCGCATCGCGCCCGCAGCTGCCGTCAGGTCCGCTGACCTCTGGGGCGGCGGCTAACGTGATTGGCGCGCTGCTGCCCGA
ACGGGCGATTGTCGTCGACGAATCGAATACCTCAGGGGTGCTGCTAGCCGCGGCTACCGCTGGAGCACCGGCCCATGATT
GGCTGACTCTGACCGGCGGGGCGATCGGTCACGGCATCCCGGCCTCGGTGGGTGCCGCGGTGGCCGCCCCCGACCGCCCT
GTGCTGTGCCTGGAATCCGACGGGTCGGCGATGTACACGATTTCGGGGTTGTGGACTCAAGCACGGGAAAACCTCGACGT
AACCACAATAATCTACAACAACGGTGCTTACAACATTCTGCGGATTGAGTTGCAACGGATGGGTGCCGGGTCGACGCCTG
GCCCCAAAGCGCTGAGTCTGCTCGATTTATCTAGCCCTACAATGGATTTCGTTAAGATCGCGGAGGGTATGGGAGTTTCT
GCTCGTCGTGTCAATACCACAGAGGGGTTGGCCGATGCCCTGCGCCTGGCCTTCACTGAACCCGGACCGCACCTGATCGA
CACGGTAGTGCCATCAATCGTGGGGTGA

Upstream 100 bases:

>100_bases
ATGCTCTAGCGGGGTTGGACAAACTCGGGCGCAGTGATCAGGCTCGCCGAGACTTCCTGCACGGCAACGCGGGACATGTT
TTTGGATTGGAGGAAAACAG

Downstream 100 bases:

>100_bases
CGCCCCCGACTCGGTTGACCGGTGAAATCGGCCACACCGAACCAGCACAGTCAAATGCCGTCGGCCGACAATTGCCGTTT
AAGCACCTTGAAGGTAACGG

Product: hypothetical protein

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNGAQAMISTLVDGGVDVCFANPGTSEMHFVAALDTVAGMRGVLTLFEGVATGAADGYARIAGRPAVVLLHLGPGLGNGL
ANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDALAGTVSGWLHRTMDTADVGVDAAEAISASLVGSQISTLILPAD
VSWSDGAIPAASVSDQPASAGVDVGAVRTALESGEPTVILIGADATGWSGLAAVARIGAATGARWFCETFPARLERGAGV
PAVERLAYFAEVAAAQLDGAKHLVLAGAQSPVSFFAYPGMPSDLVPAGCEVHVLAEHGGAAYALNALADEVASGTAAPVV
AASRPQLPSGPLTSGAAANVIGALLPERAIVVDESNTSGVLLAAATAGAPAHDWLTLTGGAIGHGIPASVGAAVAAPDRP
VLCLESDGSAMYTISGLWTQARENLDVTTIIYNNGAYNILRIELQRMGAGSTPGPKALSLLDLSSPTMDFVKIAEGMGVS
ARRVNTTEGLADALRLAFTEPGPHLIDTVVPSIVG

Sequences:

>Translated_515_residues
MNGAQAMISTLVDGGVDVCFANPGTSEMHFVAALDTVAGMRGVLTLFEGVATGAADGYARIAGRPAVVLLHLGPGLGNGL
ANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDALAGTVSGWLHRTMDTADVGVDAAEAISASLVGSQISTLILPAD
VSWSDGAIPAASVSDQPASAGVDVGAVRTALESGEPTVILIGADATGWSGLAAVARIGAATGARWFCETFPARLERGAGV
PAVERLAYFAEVAAAQLDGAKHLVLAGAQSPVSFFAYPGMPSDLVPAGCEVHVLAEHGGAAYALNALADEVASGTAAPVV
AASRPQLPSGPLTSGAAANVIGALLPERAIVVDESNTSGVLLAAATAGAPAHDWLTLTGGAIGHGIPASVGAAVAAPDRP
VLCLESDGSAMYTISGLWTQARENLDVTTIIYNNGAYNILRIELQRMGAGSTPGPKALSLLDLSSPTMDFVKIAEGMGVS
ARRVNTTEGLADALRLAFTEPGPHLIDTVVPSIVG
>Mature_515_residues
MNGAQAMISTLVDGGVDVCFANPGTSEMHFVAALDTVAGMRGVLTLFEGVATGAADGYARIAGRPAVVLLHLGPGLGNGL
ANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDALAGTVSGWLHRTMDTADVGVDAAEAISASLVGSQISTLILPAD
VSWSDGAIPAASVSDQPASAGVDVGAVRTALESGEPTVILIGADATGWSGLAAVARIGAATGARWFCETFPARLERGAGV
PAVERLAYFAEVAAAQLDGAKHLVLAGAQSPVSFFAYPGMPSDLVPAGCEVHVLAEHGGAAYALNALADEVASGTAAPVV
AASRPQLPSGPLTSGAAANVIGALLPERAIVVDESNTSGVLLAAATAGAPAHDWLTLTGGAIGHGIPASVGAAVAAPDRP
VLCLESDGSAMYTISGLWTQARENLDVTTIIYNNGAYNILRIELQRMGAGSTPGPKALSLLDLSSPTMDFVKIAEGMGVS
ARRVNTTEGLADALRLAFTEPGPHLIDTVVPSIVG

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=182, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081685, Length=115, Percent_Identity=37.3913043478261, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 51986; Mature: 51986

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAQAMISTLVDGGVDVCFANPGTSEMHFVAALDTVAGMRGVLTLFEGVATGAADGYAR
CCHHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHE
IAGRPAVVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDALAG
ECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHH
TVSGWLHRTMDTADVGVDAAEAISASLVGSQISTLILPADVSWSDGAIPAASVSDQPASA
HHHHHHHHHCCHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
GVDVGAVRTALESGEPTVILIGADATGWSGLAAVARIGAATGARWFCETFPARLERGAGV
CCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
PAVERLAYFAEVAAAQLDGAKHLVLAGAQSPVSFFAYPGMPSDLVPAGCEVHVLAEHGGA
CHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCC
AYALNALADEVASGTAAPVVAASRPQLPSGPLTSGAAANVIGALLPERAIVVDESNTSGV
EEHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCE
LLAAATAGAPAHDWLTLTGGAIGHGIPASVGAAVAAPDRPVLCLESDGSAMYTISGLWTQ
EEEEECCCCCCCCEEEEECCCCCCCCCHHCCCEEECCCCCEEEEECCCCEEEEEEHHHHH
ARENLDVTTIIYNNGAYNILRIELQRMGAGSTPGPKALSLLDLSSPTMDFVKIAEGMGVS
HHCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCC
ARRVNTTEGLADALRLAFTEPGPHLIDTVVPSIVG
EEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNGAQAMISTLVDGGVDVCFANPGTSEMHFVAALDTVAGMRGVLTLFEGVATGAADGYAR
CCHHHHHHHHHHCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHE
IAGRPAVVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDALAG
ECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHH
TVSGWLHRTMDTADVGVDAAEAISASLVGSQISTLILPADVSWSDGAIPAASVSDQPASA
HHHHHHHHHCCHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
GVDVGAVRTALESGEPTVILIGADATGWSGLAAVARIGAATGARWFCETFPARLERGAGV
CCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
PAVERLAYFAEVAAAQLDGAKHLVLAGAQSPVSFFAYPGMPSDLVPAGCEVHVLAEHGGA
CHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCC
AYALNALADEVASGTAAPVVAASRPQLPSGPLTSGAAANVIGALLPERAIVVDESNTSGV
EEHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCE
LLAAATAGAPAHDWLTLTGGAIGHGIPASVGAAVAAPDRPVLCLESDGSAMYTISGLWTQ
EEEEECCCCCCCCEEEEECCCCCCCCCHHCCCEEECCCCCEEEEECCCCEEEEEEHHHHH
ARENLDVTTIIYNNGAYNILRIELQRMGAGSTPGPKALSLLDLSSPTMDFVKIAEGMGVS
HHCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCC
ARRVNTTEGLADALRLAFTEPGPHLIDTVVPSIVG
EEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]