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The map label for this gene is est [H]

Identifier: 221229557

GI number: 221229557

Start: 400788

End: 401795

Strand: Reverse

Name: est [H]

Synonym: MLBr_00314

Alternate gene names: 221229557

Gene position: 401795-400788 (Counterclockwise)

Preceding gene: 221229558

Following gene: 221229556

Centisome position: 12.29

GC content: 60.62

Gene sequence:

>1008_bases
TTGGCCATCAGCGCCGGAGCTCCGGTCGAGATTGTCGAGTCTGGCCCAAGTATTGCTGCGCGATTAGCTTCGCTGACGTC
ACGGCTGACAATCCGGCCAATTCTGGCGGTCGGCAGCTACGTCCCGCAGCTTCCATGGCCATTCGGGTTGATCGACCATG
CTGCTCGTGTGCTACTCCCGGTCACAAACGCGGCTCGGGCCGAAGTGAACTTATCTAACACGTCAGCGCAGCTGGTCCGC
GCAGCTGGAGTGCGGCCCGCTGATGGCAGCGGGCGGATAGTTCTTTACCTGCATGGTGGAGCGTTTCTGGGGTGTGGGGC
GAACTCGCACCGTCGACTTGTCGAGACACTCTCGAAGTTGGCTGACTCCCCAATTCTGGTGGTCAACTACCGGCTGCTGC
CTAAGCACTCAATCGGGATGGCACTCGATGACTGTCACGATGGCTACCAGTGGCTGCGACGGTTGGGTTACGACCCAGAG
CAGATAGTGTTGGCGGGCGATTCCGCCGGTGGGTACCTTGCACTGGCACTTGCCCAGCGACTCCAGGACGTGGGTGAGGA
ACCAGCGGCGTTGGTGGCAATTTCACCACTGCTGCAGTTGGCTAAAAAGGGTAAGCAGGCGCACCCCAACGCCAAGACTG
ACGCAATGTTCCCGCCGAAGGCGTTCGATGCTCTTGGCAGGTTGGTTGCTAGCTCAGCCGCGAAGAACAAAGTCGACGAC
AAGCCCGAAGAGCTCTACGAACCGCTAGACCACATAGCACCGGGTCTGCCACGAACACTGATTCACGTGTCCGGATCCGA
GGTGTTGCTACACGACGCACAGTTAGCCGCGACGAAACTGGCGGCCGTAGGTGTGCCGGCCGAGATCCGCGTGTGGCCAG
GACAGGTGCACGTATTCCAGGCCGCCGCGCCGATGGTGCCCGAAGCCACCCTGTCGCTGCGCCAGATCAGCCAGTACATC
CGAGAGGCTACTGCCTCTAAAGCCTCTAGAGACAGGCAAAGCCTCTAG

Upstream 100 bases:

>100_bases
AAAGTAGAGCATGACTACGCTCAGTAAACTATCTGGCTCTCCCAGGGTTACTACGCGTCCGTGCGATGCACTGAAGGCCT
ACAGACGTGGGGCACGAGGA

Downstream 100 bases:

>100_bases
AGACAGGCCTGATATGGGATGGTGCTGGCCGCCGTCCCCGTTATCCGTTCAGCAACCCTGCAACACCCTATTCTGATGAT
TATGTTGTAGCTATGGCCCT

Product: putative esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAISAGAPVEIVESGPSIAARLASLTSRLTIRPILAVGSYVPQLPWPFGLIDHAARVLLPVTNAARAEVNLSNTSAQLVR
AAGVRPADGSGRIVLYLHGGAFLGCGANSHRRLVETLSKLADSPILVVNYRLLPKHSIGMALDDCHDGYQWLRRLGYDPE
QIVLAGDSAGGYLALALAQRLQDVGEEPAALVAISPLLQLAKKGKQAHPNAKTDAMFPPKAFDALGRLVASSAAKNKVDD
KPEELYEPLDHIAPGLPRTLIHVSGSEVLLHDAQLAATKLAAVGVPAEIRVWPGQVHVFQAAAPMVPEATLSLRQISQYI
REATASKASRDRQSL

Sequences:

>Translated_335_residues
MAISAGAPVEIVESGPSIAARLASLTSRLTIRPILAVGSYVPQLPWPFGLIDHAARVLLPVTNAARAEVNLSNTSAQLVR
AAGVRPADGSGRIVLYLHGGAFLGCGANSHRRLVETLSKLADSPILVVNYRLLPKHSIGMALDDCHDGYQWLRRLGYDPE
QIVLAGDSAGGYLALALAQRLQDVGEEPAALVAISPLLQLAKKGKQAHPNAKTDAMFPPKAFDALGRLVASSAAKNKVDD
KPEELYEPLDHIAPGLPRTLIHVSGSEVLLHDAQLAATKLAAVGVPAEIRVWPGQVHVFQAAAPMVPEATLSLRQISQYI
REATASKASRDRQSL
>Mature_334_residues
AISAGAPVEIVESGPSIAARLASLTSRLTIRPILAVGSYVPQLPWPFGLIDHAARVLLPVTNAARAEVNLSNTSAQLVRA
AGVRPADGSGRIVLYLHGGAFLGCGANSHRRLVETLSKLADSPILVVNYRLLPKHSIGMALDDCHDGYQWLRRLGYDPEQ
IVLAGDSAGGYLALALAQRLQDVGEEPAALVAISPLLQLAKKGKQAHPNAKTDAMFPPKAFDALGRLVASSAAKNKVDDK
PEELYEPLDHIAPGLPRTLIHVSGSEVLLHDAQLAATKLAAVGVPAEIRVWPGQVHVFQAAAPMVPEATLSLRQISQYIR
EATASKASRDRQSL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Caenorhabditis elegans, GI72001146, Length=380, Percent_Identity=22.1052631578947, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 35573; Mature: 35442

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISAGAPVEIVESGPSIAARLASLTSRLTIRPILAVGSYVPQLPWPFGLIDHAARVLLP
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEE
VTNAARAEVNLSNTSAQLVRAAGVRPADGSGRIVLYLHGGAFLGCGANSHRRLVETLSKL
CCCCCEEEEECCCHHHHHHHHCCCCCCCCCCEEEEEEECCEEEECCCCCHHHHHHHHHHH
ADSPILVVNYRLLPKHSIGMALDDCHDGYQWLRRLGYDPEQIVLAGDSAGGYLALALAQR
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
LQDVGEEPAALVAISPLLQLAKKGKQAHPNAKTDAMFPPKAFDALGRLVASSAAKNKVDD
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
KPEELYEPLDHIAPGLPRTLIHVSGSEVLLHDAQLAATKLAAVGVPAEIRVWPGQVHVFQ
CHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHCCCCCEEEECCCEEEEEE
AAAPMVPEATLSLRQISQYIREATASKASRDRQSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AISAGAPVEIVESGPSIAARLASLTSRLTIRPILAVGSYVPQLPWPFGLIDHAARVLLP
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEE
VTNAARAEVNLSNTSAQLVRAAGVRPADGSGRIVLYLHGGAFLGCGANSHRRLVETLSKL
CCCCCEEEEECCCHHHHHHHHCCCCCCCCCCEEEEEEECCEEEECCCCCHHHHHHHHHHH
ADSPILVVNYRLLPKHSIGMALDDCHDGYQWLRRLGYDPEQIVLAGDSAGGYLALALAQR
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
LQDVGEEPAALVAISPLLQLAKKGKQAHPNAKTDAMFPPKAFDALGRLVASSAAKNKVDD
HHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
KPEELYEPLDHIAPGLPRTLIHVSGSEVLLHDAQLAATKLAAVGVPAEIRVWPGQVHVFQ
CHHHHHHHHHHHCCCCCCEEEEECCCEEEEEHHHHHHHHHHHCCCCCEEEECCCEEEEEE
AAAPMVPEATLSLRQISQYIREATASKASRDRQSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]