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The map label for this gene is thiO/thiG [H]

Identifier: 221229550

GI number: 221229550

Start: 381671

End: 382693

Strand: Reverse

Name: thiO/thiG [H]

Synonym: MLBr_00299

Alternate gene names: 221229550

Gene position: 382693-381671 (Counterclockwise)

Preceding gene: 221229555

Following gene: 221229549

Centisome position: 11.71

GC content: 63.83

Gene sequence:

>1023_bases
ATGCCGTCAGGCCCACCCGCGGGGTCGCTGGCCGTTATCGGCGGCGGCGTCATCGGGCTATCGGTGGCACGCCGCGCAGT
GCAGGCCGGGTGGTCCGTGCGTGTACACCGGACCCACGAGCAAGGTGCGTCCTGGGTTGCCGGGGGCATGCTGGCCCCGC
ACAGCGAAGGCCGGCCCGGTGAGGAACAGTTGTTGCAGCTGGGCCTGAGGTCGCTACGGCTGTGGCACGAAGGTGGATTT
ACCGACAGGCTGGCTCCGGAGGTTATTACCGCGCGTGAGTCGCTGGTGGTGGCCGTCGACCAGGCTGATGTCGCTGACCT
ACGCACGGTTGCGGACTGGTTGTCCACGCAAGGCCACCCGGTGATCTGGGAATCTGCTGCCCGCGACATAGAACCCCTAC
TGGCACAAGGCATCCGACATGGTTTCTGGGCGCCTAACGAACTAGCAGTGGACAACCGGGCCTTGCTCGCCGGGCTGTGC
TTGGACTGTGAGCGTCTAGGGGTGCGATGGGCCTCGCCGGTCAACGATCTGTCCTGTGTCAACGCAGACGCGGTAGTGAT
TGCCAATGGCATTGGCGCTCCTGCGCTGTGGCCCGGCCTGCCGGTCCGTCCGGTCAAGGGGGAAGTGCTGCGTTTGCGCT
GGCGAAATGGTTGTATGTCATTGCTAAAGAGGTTAATTCGAGCCCATGTGCACGGCAGGCAGGTGTACCTGGTTCCGCGT
GCGGACGGAGTAGTCGTCGGTGCCACCCAGTACGAACACGGTTACGATACCGCCCCGGTCGTGTCGGGCGTGCGTGATCT
GCTTGACGATGCGTGTGCCGTGCTGCCAACGTTGGGCGAGTACGAGCTGGCTGAGTGCGCGGCTGGACTGCGCCCAACGA
CACCGAATAACCTGCCACTTGTGCACCGCCTAGATAACCGTACGCTGGTAGCTGCCGGCCATGGCAGATCGGGATTTCTG
TTGGCACCATGGACAGCTGAGCAGATCATGTTCGAACTTGCCGCGGTCGGAGCCCAAGCGTGA

Upstream 100 bases:

>100_bases
CCGAATCTGCGGGGCTGAGAGTGGGCACAGCCAGAGTGACTGAACCTACCCTTACCCTCCCACACCTGATCCGGGTCATG
CCGGCGAAGGGAGGTTCAAG

Downstream 100 bases:

>100_bases
TCGTCTTATGATTGTCGTAGTCAACGAACGACCGGTCGAGGTTAACGAGCAGACCACCGTTGCTGCGCTGTTGGAGTCTC
TGGGCTTCCCGGCTAGTGGT

Product: putative oxidoreductase

Products: NA

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MPSGPPAGSLAVIGGGVIGLSVARRAVQAGWSVRVHRTHEQGASWVAGGMLAPHSEGRPGEEQLLQLGLRSLRLWHEGGF
TDRLAPEVITARESLVVAVDQADVADLRTVADWLSTQGHPVIWESAARDIEPLLAQGIRHGFWAPNELAVDNRALLAGLC
LDCERLGVRWASPVNDLSCVNADAVVIANGIGAPALWPGLPVRPVKGEVLRLRWRNGCMSLLKRLIRAHVHGRQVYLVPR
ADGVVVGATQYEHGYDTAPVVSGVRDLLDDACAVLPTLGEYELAECAAGLRPTTPNNLPLVHRLDNRTLVAAGHGRSGFL
LAPWTAEQIMFELAAVGAQA

Sequences:

>Translated_340_residues
MPSGPPAGSLAVIGGGVIGLSVARRAVQAGWSVRVHRTHEQGASWVAGGMLAPHSEGRPGEEQLLQLGLRSLRLWHEGGF
TDRLAPEVITARESLVVAVDQADVADLRTVADWLSTQGHPVIWESAARDIEPLLAQGIRHGFWAPNELAVDNRALLAGLC
LDCERLGVRWASPVNDLSCVNADAVVIANGIGAPALWPGLPVRPVKGEVLRLRWRNGCMSLLKRLIRAHVHGRQVYLVPR
ADGVVVGATQYEHGYDTAPVVSGVRDLLDDACAVLPTLGEYELAECAAGLRPTTPNNLPLVHRLDNRTLVAAGHGRSGFL
LAPWTAEQIMFELAAVGAQA
>Mature_339_residues
PSGPPAGSLAVIGGGVIGLSVARRAVQAGWSVRVHRTHEQGASWVAGGMLAPHSEGRPGEEQLLQLGLRSLRLWHEGGFT
DRLAPEVITARESLVVAVDQADVADLRTVADWLSTQGHPVIWESAARDIEPLLAQGIRHGFWAPNELAVDNRALLAGLCL
DCERLGVRWASPVNDLSCVNADAVVIANGIGAPALWPGLPVRPVKGEVLRLRWRNGCMSLLKRLIRAHVHGRQVYLVPRA
DGVVVGATQYEHGYDTAPVVSGVRDLLDDACAVLPTLGEYELAECAAGLRPTTPNNLPLVHRLDNRTLVAAGHGRSGFLL
APWTAEQIMFELAAVGAQA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 36312; Mature: 36181

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSGPPAGSLAVIGGGVIGLSVARRAVQAGWSVRVHRTHEQGASWVAGGMLAPHSEGRPG
CCCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECHHCCCCCHHCCCCCCCCCCCCC
EEQLLQLGLRSLRLWHEGGFTDRLAPEVITARESLVVAVDQADVADLRTVADWLSTQGHP
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
VIWESAARDIEPLLAQGIRHGFWAPNELAVDNRALLAGLCLDCERLGVRWASPVNDLSCV
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHC
NADAVVIANGIGAPALWPGLPVRPVKGEVLRLRWRNGCMSLLKRLIRAHVHGRQVYLVPR
CCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEC
ADGVVVGATQYEHGYDTAPVVSGVRDLLDDACAVLPTLGEYELAECAAGLRPTTPNNLPL
CCCEEEECEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCE
VHRLDNRTLVAAGHGRSGFLLAPWTAEQIMFELAAVGAQA
EEECCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSGPPAGSLAVIGGGVIGLSVARRAVQAGWSVRVHRTHEQGASWVAGGMLAPHSEGRPG
CCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEEECHHCCCCCHHCCCCCCCCCCCCC
EEQLLQLGLRSLRLWHEGGFTDRLAPEVITARESLVVAVDQADVADLRTVADWLSTQGHP
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
VIWESAARDIEPLLAQGIRHGFWAPNELAVDNRALLAGLCLDCERLGVRWASPVNDLSCV
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHC
NADAVVIANGIGAPALWPGLPVRPVKGEVLRLRWRNGCMSLLKRLIRAHVHGRQVYLVPR
CCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEC
ADGVVVGATQYEHGYDTAPVVSGVRDLLDDACAVLPTLGEYELAECAAGLRPTTPNNLPL
CCCEEEECEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCE
VHRLDNRTLVAAGHGRSGFLLAPWTAEQIMFELAAVGAQA
EEECCCCEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]