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The map label for this gene is thiG

Identifier: 221229548

GI number: 221229548

Start: 380661

End: 381446

Strand: Reverse

Name: thiG

Synonym: MLBr_00297

Alternate gene names: 221229548

Gene position: 381446-380661 (Counterclockwise)

Preceding gene: 221229549

Following gene: 221229545

Centisome position: 11.67

GC content: 59.29

Gene sequence:

>786_bases
GTGGTTGAATCAAAATTCACAATTGGGGACCGCACCTTCACCTCGCGGCTCATCATGGGTACCGGGGGAGCCGCTAACTT
GGCTATATTAGAAGAGGCGTTGATCGCCTCAGGCACCGAGCTGACGACAGTAGCGATCCGCCGGGTCGACACCGATGGTG
GATCCGGATTGCTCAAGCTACTCAGCCGGCTTGACATCATGCCGCTGCCCAATACCGCCGGGTGTCGCAGCGCTGCAGAG
GCGGTGTTGACCGCACAACTGGCCCGCGAGGCGCTGAACACCAACTGGATCAAACTCGAAGTGATCGCAGACGAACGCAC
CCTACTGCCGGATGGACTTGAATTAGTAAGAGCAGCAGAACAATTAGTTGACGCTGGCTTTGTGGTTCTGCCGTATACTA
ACGACGACCCTGCGCTGGCTCATAGACTAGAGGGCACTGGCTGCGCGGCAGTGATGCCGCTAGGTTCGCCGATTGGCACT
GGCCTCGGCATCAACAATCCACACAATATCGAGATTATCGTAGCGCAGGCTAGAGTCCCGGTGGTGCTCGACGCCGGAAT
CGGTACTACAAGCGACGCTGCCTTAGCGATGGAATTAGGCTGCGACGCTGTGCTATTGGCTTCTGCGGTGACTCGAGCCG
TCGACCCTCCGACCATGGCCGCCGCGATGGCCTCTGCTGTGACCGCCGGCTATCTTGCACGCCGGGCCGGGAGGATCCCA
AAACGCTTCTGGGCGCAGGCTTCTAGCCCGGAACTGATGCGGACCGGTGAGGAGTTGGGCAATTGA

Upstream 100 bases:

>100_bases
GTGTTGCCTCGATCTTACTGGGCAACAAAAATTTCTGAGCTCCCCGCTGTTACTGGGAGGTCTGAGCCAATCAGACTCGA
AGTGGTTACGGCAGTGCAAG

Downstream 100 bases:

>100_bases
ACTTCCTCCCGGAGGTGAGCCGGTATATTGCACTGGGTAGTTCGATGGTGGATGGTACCAATATTCGTTTCCCGTGCTGC
TGGCGTGCCATGTAGATTAG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MVESKFTIGDRTFTSRLIMGTGGAANLAILEEALIASGTELTTVAIRRVDTDGGSGLLKLLSRLDIMPLPNTAGCRSAAE
AVLTAQLAREALNTNWIKLEVIADERTLLPDGLELVRAAEQLVDAGFVVLPYTNDDPALAHRLEGTGCAAVMPLGSPIGT
GLGINNPHNIEIIVAQARVPVVLDAGIGTTSDAALAMELGCDAVLLASAVTRAVDPPTMAAAMASAVTAGYLARRAGRIP
KRFWAQASSPELMRTGEELGN

Sequences:

>Translated_261_residues
MVESKFTIGDRTFTSRLIMGTGGAANLAILEEALIASGTELTTVAIRRVDTDGGSGLLKLLSRLDIMPLPNTAGCRSAAE
AVLTAQLAREALNTNWIKLEVIADERTLLPDGLELVRAAEQLVDAGFVVLPYTNDDPALAHRLEGTGCAAVMPLGSPIGT
GLGINNPHNIEIIVAQARVPVVLDAGIGTTSDAALAMELGCDAVLLASAVTRAVDPPTMAAAMASAVTAGYLARRAGRIP
KRFWAQASSPELMRTGEELGN
>Mature_261_residues
MVESKFTIGDRTFTSRLIMGTGGAANLAILEEALIASGTELTTVAIRRVDTDGGSGLLKLLSRLDIMPLPNTAGCRSAAE
AVLTAQLAREALNTNWIKLEVIADERTLLPDGLELVRAAEQLVDAGFVVLPYTNDDPALAHRLEGTGCAAVMPLGSPIGT
GLGINNPHNIEIIVAQARVPVVLDAGIGTTSDAALAMELGCDAVLLASAVTRAVDPPTMAAAMASAVTAGYLARRAGRIP
KRFWAQASSPELMRTGEELGN

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=244, Percent_Identity=51.2295081967213, Blast_Score=231, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_MYCLB (B8ZU92)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502964.1
- ProteinModelPortal:   B8ZU92
- SMR:   B8ZU92
- EnsemblBacteria:   EBMYCT00000085679
- GeneID:   7326363
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000015733
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27139; Mature: 27139

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: ACT_SITE 98-98 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVESKFTIGDRTFTSRLIMGTGGAANLAILEEALIASGTELTTVAIRRVDTDGGSGLLKL
CCCCCEECCCHHHHHEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHH
LSRLDIMPLPNTAGCRSAAEAVLTAQLAREALNTNWIKLEVIADERTLLPDGLELVRAAE
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
QLVDAGFVVLPYTNDDPALAHRLEGTGCAAVMPLGSPIGTGLGINNPHNIEIIVAQARVP
HHHHCCEEEEEECCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCC
VVLDAGIGTTSDAALAMELGCDAVLLASAVTRAVDPPTMAAAMASAVTAGYLARRAGRIP
EEEECCCCCCCCCCEEHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
KRFWAQASSPELMRTGEELGN
HHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MVESKFTIGDRTFTSRLIMGTGGAANLAILEEALIASGTELTTVAIRRVDTDGGSGLLKL
CCCCCEECCCHHHHHEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHH
LSRLDIMPLPNTAGCRSAAEAVLTAQLAREALNTNWIKLEVIADERTLLPDGLELVRAAE
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
QLVDAGFVVLPYTNDDPALAHRLEGTGCAAVMPLGSPIGTGLGINNPHNIEIIVAQARVP
HHHHCCEEEEEECCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCC
VVLDAGIGTTSDAALAMELGCDAVLLASAVTRAVDPPTMAAAMASAVTAGYLARRAGRIP
EEEECCCCCCCCCCEEHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
KRFWAQASSPELMRTGEELGN
HHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA