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The map label for this gene is purA

Identifier: 221229536

GI number: 221229536

Start: 361966

End: 363264

Strand: Reverse

Name: purA

Synonym: MLBr_00280

Alternate gene names: 221229536

Gene position: 363264-361966 (Counterclockwise)

Preceding gene: 221229539

Following gene: 221229535

Centisome position: 11.12

GC content: 60.74

Gene sequence:

>1299_bases
ATGCCGGCAGTCGTCCTCATCGGCGCTCAGTGGGGCGACGAGGGCAAAGGTAAGGTCACAGACCTGCTCGGCGGACGTGC
CCAGTGGGTGGTGCGCTATCAGGGTGGCAATAACGCTGGGCATACCGTTGTCTTGCCTACTGGCGAGAACTTCACACTGC
ATCTGATCCCGTCGGGGGTGCTGACGCCGGGTGTTACCAACGTCATCGGTAACGGTGTGGTGGTGGATCCCGGAGTGCTG
CTCAGCGAACTGCAAGGCCTGGAAGATCGTGGTGTCGACACCTCCCAGCTGCTGATCTCAGCCGATGCGCACCTGCTGAT
GCCCTACCACGTGGCCATCGACAAGGTCACCGAGCGTTACATGGGCAACAAGAAGATCGGCACTACCGGCCGCGGTATCG
GGCCCTGTTACCAGGACAAGATCGCCCGCATGGGGATACGGGTCGCCGATGTCTTAGAACCTGGTGAGTTGACCCACAAG
ATCGAGGCTGCGCTCGAGTTCAAAAACCAGGTGTTGGTCAAGATCTACAACCGCAAGGCGTTGGACCTGGCTCAGGTTGT
CGAAACGCTGCTGGAGCAGGCGCAGCAATTTCGACATCGCATCACCGACACTCGTCTGTTGCTCAACGACGCCCTCGAGG
CCGGTGAGACGGTGCTGCTTGAAGGCGCACAAGGCACCCTGCTCGACGTTGATCACGGCACCTACCCGTATGTGACATCG
TCGAATCCGACGGCGGGCGGTGCGGCGTTGGGCTCCGGTATCGGACCGACCCGGATCCACACTGTGCTAGGCATTCTCAA
GGCCTACACTACTCGGGTGGGTTCTGGTCCTTTCCCTACCGAACTGTTCGACGAGAACGGCGAGTACTTGGCCAAGACTG
GTAGCGAGATCGGCGTGACGACAGGACGACGGCGGCGTTGCGGGTGGTTCGACGCCGTGATAGCCCGCTACGCCACCCGG
GTCAACGGGATCACCGACTATTTCCTGACCAAGCTTGACGTGCTGTCCAGCTTGGAAACGGTGCCGGTCTGCGTCGGCTA
TCAGATCGCCGGTGTGCGCACCCACGATATGCCGATAACCCAAAGCGATCTCGCCCGTGCCGAGCCGATCTATGAGGAGC
TGCCGGGTTGGTGGGAAGACATCTCCGGAGCGCGGGAATTCGAGGACCTGCCCGCCAAAGCGCGTGACTACGTATTGAGA
CTGGAAGAACTTGCTGGAGCACAGGTTGCGTGCATAGGTGTCGGTCCGGGACGGGATCAGACCATCGTGCGATGCGATGT
ATTGAGGAGCCGTCGGTGA

Upstream 100 bases:

>100_bases
CGGGCCGAGCACGGCTCGCATCATTGCAGGGCTAAGTTGATCACCGGCTGGACCACAATAAGCAGGCGTAAACACGCGGG
ATTTAGGTGGGAGCAAGTCA

Downstream 100 bases:

>100_bases
CCTACTATTCTTCCCTGCGCCCAGAAGACCTACCTCCAGAACGCCCTAAACACGAACACTATAGCGGCTTTCCTGAATAC
GAACTGGCTAACCCTGGTGT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPAVVLIGAQWGDEGKGKVTDLLGGRAQWVVRYQGGNNAGHTVVLPTGENFTLHLIPSGVLTPGVTNVIGNGVVVDPGVL
LSELQGLEDRGVDTSQLLISADAHLLMPYHVAIDKVTERYMGNKKIGTTGRGIGPCYQDKIARMGIRVADVLEPGELTHK
IEAALEFKNQVLVKIYNRKALDLAQVVETLLEQAQQFRHRITDTRLLLNDALEAGETVLLEGAQGTLLDVDHGTYPYVTS
SNPTAGGAALGSGIGPTRIHTVLGILKAYTTRVGSGPFPTELFDENGEYLAKTGSEIGVTTGRRRRCGWFDAVIARYATR
VNGITDYFLTKLDVLSSLETVPVCVGYQIAGVRTHDMPITQSDLARAEPIYEELPGWWEDISGAREFEDLPAKARDYVLR
LEELAGAQVACIGVGPGRDQTIVRCDVLRSRR

Sequences:

>Translated_432_residues
MPAVVLIGAQWGDEGKGKVTDLLGGRAQWVVRYQGGNNAGHTVVLPTGENFTLHLIPSGVLTPGVTNVIGNGVVVDPGVL
LSELQGLEDRGVDTSQLLISADAHLLMPYHVAIDKVTERYMGNKKIGTTGRGIGPCYQDKIARMGIRVADVLEPGELTHK
IEAALEFKNQVLVKIYNRKALDLAQVVETLLEQAQQFRHRITDTRLLLNDALEAGETVLLEGAQGTLLDVDHGTYPYVTS
SNPTAGGAALGSGIGPTRIHTVLGILKAYTTRVGSGPFPTELFDENGEYLAKTGSEIGVTTGRRRRCGWFDAVIARYATR
VNGITDYFLTKLDVLSSLETVPVCVGYQIAGVRTHDMPITQSDLARAEPIYEELPGWWEDISGAREFEDLPAKARDYVLR
LEELAGAQVACIGVGPGRDQTIVRCDVLRSRR
>Mature_431_residues
PAVVLIGAQWGDEGKGKVTDLLGGRAQWVVRYQGGNNAGHTVVLPTGENFTLHLIPSGVLTPGVTNVIGNGVVVDPGVLL
SELQGLEDRGVDTSQLLISADAHLLMPYHVAIDKVTERYMGNKKIGTTGRGIGPCYQDKIARMGIRVADVLEPGELTHKI
EAALEFKNQVLVKIYNRKALDLAQVVETLLEQAQQFRHRITDTRLLLNDALEAGETVLLEGAQGTLLDVDHGTYPYVTSS
NPTAGGAALGSGIGPTRIHTVLGILKAYTTRVGSGPFPTELFDENGEYLAKTGSEIGVTTGRRRRCGWFDAVIARYATRV
NGITDYFLTKLDVLSSLETVPVCVGYQIAGVRTHDMPITQSDLARAEPIYEELPGWWEDISGAREFEDLPAKARDYVLRL
EELAGAQVACIGVGPGRDQTIVRCDVLRSRR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=44.3661971830986, Blast_Score=327, Evalue=9e-90,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=42.5233644859813, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=42.8211586901763, Blast_Score=291, Evalue=9e-79,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=51.0588235294118, Blast_Score=435, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=45.1612903225806, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=45.1612903225806, Blast_Score=349, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=42.093023255814, Blast_Score=347, Evalue=3e-96,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=43.2624113475177, Blast_Score=322, Evalue=3e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_MYCLB (B8ZU80)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502952.1
- ProteinModelPortal:   B8ZU80
- SMR:   B8ZU80
- EnsemblBacteria:   EBMYCT00000086402
- GeneID:   7326351
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000016822
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46794; Mature: 46663

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 224-224 BINDING 239-239 BINDING 303-303 BINDING 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVVLIGAQWGDEGKGKVTDLLGGRAQWVVRYQGGNNAGHTVVLPTGENFTLHLIPSGV
CCEEEEEECCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCC
LTPGVTNVIGNGVVVDPGVLLSELQGLEDRGVDTSQLLISADAHLLMPYHVAIDKVTERY
CCCCHHHHHCCCEEECCHHHHHHHCCHHHCCCCHHHEEEECCCEEEEHHHHHHHHHHHHH
MGNKKIGTTGRGIGPCYQDKIARMGIRVADVLEPGELTHKIEAALEFKNQVLVKIYNRKA
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHEEEEEECCCCH
LDLAQVVETLLEQAQQFRHRITDTRLLLNDALEAGETVLLEGAQGTLLDVDHGTYPYVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTAGGAALGSGIGPTRIHTVLGILKAYTTRVGSGPFPTELFDENGEYLAKTGSEIGVT
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEECCCCCCCCC
TGRRRRCGWFDAVIARYATRVNGITDYFLTKLDVLSSLETVPVCVGYQIAGVRTHDMPIT
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECEEEECEEECCCCCC
QSDLARAEPIYEELPGWWEDISGAREFEDLPAKARDYVLRLEELAGAQVACIGVGPGRDQ
HHHHHHCCCHHHHCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCC
TIVRCDVLRSRR
EEEEEEHHHCCC
>Mature Secondary Structure 
PAVVLIGAQWGDEGKGKVTDLLGGRAQWVVRYQGGNNAGHTVVLPTGENFTLHLIPSGV
CEEEEEECCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCC
LTPGVTNVIGNGVVVDPGVLLSELQGLEDRGVDTSQLLISADAHLLMPYHVAIDKVTERY
CCCCHHHHHCCCEEECCHHHHHHHCCHHHCCCCHHHEEEECCCEEEEHHHHHHHHHHHHH
MGNKKIGTTGRGIGPCYQDKIARMGIRVADVLEPGELTHKIEAALEFKNQVLVKIYNRKA
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHEEEEEECCCCH
LDLAQVVETLLEQAQQFRHRITDTRLLLNDALEAGETVLLEGAQGTLLDVDHGTYPYVTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTAGGAALGSGIGPTRIHTVLGILKAYTTRVGSGPFPTELFDENGEYLAKTGSEIGVT
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEECCCCCCCCC
TGRRRRCGWFDAVIARYATRVNGITDYFLTKLDVLSSLETVPVCVGYQIAGVRTHDMPIT
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECEEEECEEECCCCCC
QSDLARAEPIYEELPGWWEDISGAREFEDLPAKARDYVLRLEELAGAQVACIGVGPGRDQ
HHHHHHCCCHHHHCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCC
TIVRCDVLRSRR
EEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA