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The map label for this gene is mscL

Identifier: 221229469

GI number: 221229469

Start: 244802

End: 245266

Strand: Reverse

Name: mscL

Synonym: MLBr_00178

Alternate gene names: 221229469

Gene position: 245266-244802 (Counterclockwise)

Preceding gene: 221229470

Following gene: 221229462

Centisome position: 7.5

GC content: 54.41

Gene sequence:

>465_bases
ATGTTCAGAGGGTTCAAGGAGTTTCTCTCACGAGGCAATATCGTCGACCTGGCCGTAGCGGTGGTTATCGGTACCGCGTT
CACCGCGTTGATAACCAAGTTCACCGACAGCATCATCACGCCATTGATCAATCGGGTCGGCGTCAACCAACAAACCAATA
TCAGCCCCTTGAGGATCGACATAGGGGGAGATCAGGCCATCGACTTGAACATCGTGCTCTCGGCCGCGATCAACTTCTTG
CTGATCGCTTTGGTAGTGTATTTCCTGGTCGTGTTGCCCTACACCACGATACGCAAGCATGGTGAGGTCGAACAATTCGA
CACCGACCTAATCGGGAACCAAGTTGTCCTACTCGCCGAAATCCGTGATCTTCTCGCCCAAAGTAACGGGGCTCCGTCCG
GCAGGCACGTCGATACAGCCGATCTGACGCCAACCCCAAACCACGAACCTCGCGCAGACACATGA

Upstream 100 bases:

>100_bases
GCTCGGTTAGATGGTGACTTTGACCCTGCAACTGAATGCCTGGCAATCGCTCAACACATGACACACAGCAAGCTGTTGTC
CAGCCCAGAAAGGACCTCCC

Downstream 100 bases:

>100_bases
CCGCAGCGGCGCCAGCCTAGATTTCCAGACTCGATAATTGCCCAATGATCTGGGTGGCCAGCGGATTCAATGTAGCCATT
CCGTCGCGCACCGCATAACG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MFRGFKEFLSRGNIVDLAVAVVIGTAFTALITKFTDSIITPLINRVGVNQQTNISPLRIDIGGDQAIDLNIVLSAAINFL
LIALVVYFLVVLPYTTIRKHGEVEQFDTDLIGNQVVLLAEIRDLLAQSNGAPSGRHVDTADLTPTPNHEPRADT

Sequences:

>Translated_154_residues
MFRGFKEFLSRGNIVDLAVAVVIGTAFTALITKFTDSIITPLINRVGVNQQTNISPLRIDIGGDQAIDLNIVLSAAINFL
LIALVVYFLVVLPYTTIRKHGEVEQFDTDLIGNQVVLLAEIRDLLAQSNGAPSGRHVDTADLTPTPNHEPRADT
>Mature_154_residues
MFRGFKEFLSRGNIVDLAVAVVIGTAFTALITKFTDSIITPLINRVGVNQQTNISPLRIDIGGDQAIDLNIVLSAAINFL
LIALVVYFLVVLPYTTIRKHGEVEQFDTDLIGNQVVLLAEIRDLLAQSNGAPSGRHVDTADLTPTPNHEPRADT

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_MYCLB (B8ZU13)

Other databases:

- EMBL:   FM211192
- RefSeq:   YP_002502885.1
- ProteinModelPortal:   B8ZU13
- SMR:   B8ZU13
- EnsemblBacteria:   EBMYCT00000086094
- GeneID:   7324912
- GenomeReviews:   FM211192_GR
- GeneTree:   EBGT00050000017393
- HOGENOM:   HBG727604
- OMA:   AIVTAFT
- ProtClustDB:   PRK00567
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089
- Gene3D:   G3DSA:1.20.5.220
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16752; Mature: 16752

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x218bb3a8)-; HASH(0x22868cbc)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRGFKEFLSRGNIVDLAVAVVIGTAFTALITKFTDSIITPLINRVGVNQQTNISPLRID
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IGGDQAIDLNIVLSAAINFLLIALVVYFLVVLPYTTIRKHGEVEQFDTDLIGNQVVLLAE
ECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHCCCCHHHHH
IRDLLAQSNGAPSGRHVDTADLTPTPNHEPRADT
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MFRGFKEFLSRGNIVDLAVAVVIGTAFTALITKFTDSIITPLINRVGVNQQTNISPLRID
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
IGGDQAIDLNIVLSAAINFLLIALVVYFLVVLPYTTIRKHGEVEQFDTDLIGNQVVLLAE
ECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHCCCCHHHHH
IRDLLAQSNGAPSGRHVDTADLTPTPNHEPRADT
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA