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The map label for this gene is fadD28 [H]

Identifier: 221229447

GI number: 221229447

Start: 192040

End: 193779

Strand: Reverse

Name: fadD28 [H]

Synonym: MLBr_00138

Alternate gene names: 221229447

Gene position: 193779-192040 (Counterclockwise)

Preceding gene: 221229449

Following gene: 221229446

Centisome position: 5.93

GC content: 54.89

Gene sequence:

>1740_bases
ATGAGTGTGCGTTCCATCCCCGCCGTGCTGCGCACGTGCGCACGTCTACAACCGAACGACCCGGTCTTCACGTTTATGGA
TTACGAACAGGACTGGGACGGTGTCAAGATAACCTTGACGTGGTCGCAGCTGTACCGACGAACGCTGAACGTGGCACAAG
AATTGAACCGTTGTGGTTCAACGGGTGATCGGGTCGCCATCTCCGCTCCACAAGGACTCGACTACGTTGTCGCTTTTCTC
GGCGCATTGCAAGCCGGGCGTATCGCTGTTCCCCTGTCGGTGCCACAGGGCGGCGTCACGGATGAACGTTTCGAGTCAGT
GCTCAACGATTCATCGCCTGTGGCCATCCTCACAACATCGCCCGCCGTGGACGATGTTGTGCATCACGTTACGCAGCGTC
CTGATGAATCTACGCCATCAATCATTGAAGTTGATTTGCTAGATCTTGACTCGCCGAACGGGTCCGGCGACGGTAACGGC
GACCACCTATCCACCGCCTATTTGCAATACACTTCAGGGTCGACTCGCACTCCCGCGGGAGTGATGATATCGCATCACAA
CCTTCGGGCCAATTTCGAACAGCTGATGTCGACCTACTTGGCGGACACTGACGGGGTCTTCCCGGTGGGTGGGATGAGCG
TATCGTGGCTACCGTTCTACCACGACATGGGTCTGATACTGGGAGTTTGCGTACCGATTTTAGGGGGATGTCCGGCAGTA
CTCACCAGTCCAGTGTCGTTCCTACAGCGCCCAGCCCGGTGGATGCACATGTTAGCCAGTGAGTTTCCAGTCTTTTCGGC
GGCACCGAACTTCGCCTTTGAACTGGCGACAAAGAAGATATCGGACGACGATATGATCGGGGTCGACCTAGGTAATATAC
ACATTATCCTGAATGGTAGCGAACGAGTACAACCCGCGACGATTAAGCGCTTCAACAACCGTTTTGCTCGTTTCAATTTG
AAGGAAAAAGCGGTTCGGCCTTCATACGGGCTCGCAGAAGCAACAGTGTACGTAGCAACCAGCAGATCGGGCCAGCCCGC
GGAACTCGTTGACTTCGATGCTGAGAGTTTGTCCACCGGCCAGGCCAAGACTTGCACAAGTGGCAACGGTACATCGTTGA
TCAGCTACGTTTTGCCGCCGTCACCGATCGTACGGATTGTCGACTCGGACACCTGCATACAATGTCAAGATGGAACCGTC
GGTGAGATTTGGGTACACGGTGACAACGTCGCTGACGGCTATTGGCAAAAACCTGACGAGAGTGAGCGCACGTTCGGCGG
AAAGATTGCCGCCCCTTCGAGAGGAACACCCGAAGGTCCTTGGTTGAGAACGGGAGACTCGGGCTTCGTAATCGACGGCA
AGATGTTCATCATTGGCCGTATAAAGGATCTGCTAATCGTGTACGGCCGCAACCATTCCCCGGACGATATTGAGGGGACG
ATCCAGGAAATCACCCGAGGCCGCTGCGCGGCAATATCGGTTCCTGACCGCAACACCGAAAAACTAGTTGCCATAATCGA
GCTCAAGAAGCTGGGTGACGCAGACCAGTATGCGATGGACCGACTAGGCGCTATCAAACGTGAAGTCATCTCGGCTTTAT
CAAATTCACACGGACTAAGCGTCTCAGATGTAGTCCTGGTAGCGCCTGGCTCGATCCCGATTACCACAAGCGGCAAAGTC
AGACGATCGACATGTGTCGAACGGTATCGACAGGATCAGTTCGACCGCCTAGATGCTTAG

Upstream 100 bases:

>100_bases
TCTGGCCAAAATGTGATTTCGCCGACACTGTTGAGCCGGCACGTGAAGTTATTCGGCAACTTGCGCCGACGTCAGAATCT
AGTCCAGTAACGTTATCGCC

Downstream 100 bases:

>100_bases
CCAAAGACCCTACTGTGTAGAATTTAGTACATCCATCTGAGATTCACGAGCGTGTCAACCGACGACAGTCATAGGTCGCT
CCGGCTAGCCAACCTCCTTG

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MSVRSIPAVLRTCARLQPNDPVFTFMDYEQDWDGVKITLTWSQLYRRTLNVAQELNRCGSTGDRVAISAPQGLDYVVAFL
GALQAGRIAVPLSVPQGGVTDERFESVLNDSSPVAILTTSPAVDDVVHHVTQRPDESTPSIIEVDLLDLDSPNGSGDGNG
DHLSTAYLQYTSGSTRTPAGVMISHHNLRANFEQLMSTYLADTDGVFPVGGMSVSWLPFYHDMGLILGVCVPILGGCPAV
LTSPVSFLQRPARWMHMLASEFPVFSAAPNFAFELATKKISDDDMIGVDLGNIHIILNGSERVQPATIKRFNNRFARFNL
KEKAVRPSYGLAEATVYVATSRSGQPAELVDFDAESLSTGQAKTCTSGNGTSLISYVLPPSPIVRIVDSDTCIQCQDGTV
GEIWVHGDNVADGYWQKPDESERTFGGKIAAPSRGTPEGPWLRTGDSGFVIDGKMFIIGRIKDLLIVYGRNHSPDDIEGT
IQEITRGRCAAISVPDRNTEKLVAIIELKKLGDADQYAMDRLGAIKREVISALSNSHGLSVSDVVLVAPGSIPITTSGKV
RRSTCVERYRQDQFDRLDA

Sequences:

>Translated_579_residues
MSVRSIPAVLRTCARLQPNDPVFTFMDYEQDWDGVKITLTWSQLYRRTLNVAQELNRCGSTGDRVAISAPQGLDYVVAFL
GALQAGRIAVPLSVPQGGVTDERFESVLNDSSPVAILTTSPAVDDVVHHVTQRPDESTPSIIEVDLLDLDSPNGSGDGNG
DHLSTAYLQYTSGSTRTPAGVMISHHNLRANFEQLMSTYLADTDGVFPVGGMSVSWLPFYHDMGLILGVCVPILGGCPAV
LTSPVSFLQRPARWMHMLASEFPVFSAAPNFAFELATKKISDDDMIGVDLGNIHIILNGSERVQPATIKRFNNRFARFNL
KEKAVRPSYGLAEATVYVATSRSGQPAELVDFDAESLSTGQAKTCTSGNGTSLISYVLPPSPIVRIVDSDTCIQCQDGTV
GEIWVHGDNVADGYWQKPDESERTFGGKIAAPSRGTPEGPWLRTGDSGFVIDGKMFIIGRIKDLLIVYGRNHSPDDIEGT
IQEITRGRCAAISVPDRNTEKLVAIIELKKLGDADQYAMDRLGAIKREVISALSNSHGLSVSDVVLVAPGSIPITTSGKV
RRSTCVERYRQDQFDRLDA
>Mature_578_residues
SVRSIPAVLRTCARLQPNDPVFTFMDYEQDWDGVKITLTWSQLYRRTLNVAQELNRCGSTGDRVAISAPQGLDYVVAFLG
ALQAGRIAVPLSVPQGGVTDERFESVLNDSSPVAILTTSPAVDDVVHHVTQRPDESTPSIIEVDLLDLDSPNGSGDGNGD
HLSTAYLQYTSGSTRTPAGVMISHHNLRANFEQLMSTYLADTDGVFPVGGMSVSWLPFYHDMGLILGVCVPILGGCPAVL
TSPVSFLQRPARWMHMLASEFPVFSAAPNFAFELATKKISDDDMIGVDLGNIHIILNGSERVQPATIKRFNNRFARFNLK
EKAVRPSYGLAEATVYVATSRSGQPAELVDFDAESLSTGQAKTCTSGNGTSLISYVLPPSPIVRIVDSDTCIQCQDGTVG
EIWVHGDNVADGYWQKPDESERTFGGKIAAPSRGTPEGPWLRTGDSGFVIDGKMFIIGRIKDLLIVYGRNHSPDDIEGTI
QEITRGRCAAISVPDRNTEKLVAIIELKKLGDADQYAMDRLGAIKREVISALSNSHGLSVSDVVLVAPGSIPITTSGKVR
RSTCVERYRQDQFDRLDA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=603, Percent_Identity=25.5389718076285, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI225735629, Length=607, Percent_Identity=24.7116968698517, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI45827692, Length=607, Percent_Identity=24.7116968698517, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI55749758, Length=580, Percent_Identity=24.4827586206897, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI225735625, Length=621, Percent_Identity=23.0273752012882, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI45827694, Length=561, Percent_Identity=23.1729055258467, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788107, Length=516, Percent_Identity=27.3255813953488, Blast_Score=112, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI193202679, Length=602, Percent_Identity=23.7541528239203, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24654656, Length=466, Percent_Identity=24.2489270386266, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18859661, Length=535, Percent_Identity=23.9252336448598, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62836; Mature: 62704

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRSIPAVLRTCARLQPNDPVFTFMDYEQDWDGVKITLTWSQLYRRTLNVAQELNRCGS
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
TGDRVAISAPQGLDYVVAFLGALQAGRIAVPLSVPQGGVTDERFESVLNDSSPVAILTTS
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECC
PAVDDVVHHVTQRPDESTPSIIEVDLLDLDSPNGSGDGNGDHLSTAYLQYTSGSTRTPAG
CCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
VMISHHNLRANFEQLMSTYLADTDGVFPVGGMSVSWLPFYHDMGLILGVCVPILGGCPAV
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHH
LTSPVSFLQRPARWMHMLASEFPVFSAAPNFAFELATKKISDDDMIGVDLGNIHIILNGS
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEHHHCCCCCCEEEEEECCEEEEECCC
ERVQPATIKRFNNRFARFNLKEKAVRPSYGLAEATVYVATSRSGQPAELVDFDAESLSTG
CCCCHHHHHHHHCHHEECCCHHHCCCCCCCCCEEEEEEEECCCCCCCCEEECCHHHCCCC
QAKTCTSGNGTSLISYVLPPSPIVRIVDSDTCIQCQDGTVGEIWVHGDNVADGYWQKPDE
CCCEECCCCCCEEEEEECCCCCEEEEECCCCEEEECCCCEEEEEEECCCCCCCCCCCCCC
SERTFGGKIAAPSRGTPEGPWLRTGDSGFVIDGKMFIIGRIKDLLIVYGRNHSPDDIEGT
CCCCCCCEECCCCCCCCCCCEEEECCCCEEEECCEEEEEEECEEEEEECCCCCCCHHHHH
IQEITRGRCAAISVPDRNTEKLVAIIELKKLGDADQYAMDRLGAIKREVISALSNSHGLS
HHHHCCCCEEEEECCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VSDVVLVAPGSIPITTSGKVRRSTCVERYRQDQFDRLDA
EEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVRSIPAVLRTCARLQPNDPVFTFMDYEQDWDGVKITLTWSQLYRRTLNVAQELNRCGS
CCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
TGDRVAISAPQGLDYVVAFLGALQAGRIAVPLSVPQGGVTDERFESVLNDSSPVAILTTS
CCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECC
PAVDDVVHHVTQRPDESTPSIIEVDLLDLDSPNGSGDGNGDHLSTAYLQYTSGSTRTPAG
CCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
VMISHHNLRANFEQLMSTYLADTDGVFPVGGMSVSWLPFYHDMGLILGVCVPILGGCPAV
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHH
LTSPVSFLQRPARWMHMLASEFPVFSAAPNFAFELATKKISDDDMIGVDLGNIHIILNGS
HHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEHHHCCCCCCEEEEEECCEEEEECCC
ERVQPATIKRFNNRFARFNLKEKAVRPSYGLAEATVYVATSRSGQPAELVDFDAESLSTG
CCCCHHHHHHHHCHHEECCCHHHCCCCCCCCCEEEEEEEECCCCCCCCEEECCHHHCCCC
QAKTCTSGNGTSLISYVLPPSPIVRIVDSDTCIQCQDGTVGEIWVHGDNVADGYWQKPDE
CCCEECCCCCCEEEEEECCCCCEEEEECCCCEEEECCCCEEEEEEECCCCCCCCCCCCCC
SERTFGGKIAAPSRGTPEGPWLRTGDSGFVIDGKMFIIGRIKDLLIVYGRNHSPDDIEGT
CCCCCCCEECCCCCCCCCCCEEEECCCCEEEECCEEEEEEECEEEEEECCCCCCCHHHHH
IQEITRGRCAAISVPDRNTEKLVAIIELKKLGDADQYAMDRLGAIKREVISALSNSHGLS
HHHHCCCCEEEEECCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VSDVVLVAPGSIPITTSGKVRRSTCVERYRQDQFDRLDA
EEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972; 1527058 [H]