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The map label for this gene is echA1 [H]

Identifier: 221229431

GI number: 221229431

Start: 161768

End: 162604

Strand: Reverse

Name: echA1 [H]

Synonym: MLBr_00120

Alternate gene names: 221229431

Gene position: 162604-161768 (Counterclockwise)

Preceding gene: 221229432

Following gene: 221229430

Centisome position: 4.98

GC content: 61.17

Gene sequence:

>837_bases
ATGGGCTCAGACATAAATCGGGCATATGAATCCGTTGCCGTCGAAATCAAAGATCGGGTTGCACAGGTAACACTGATCGG
GCCAGGCAAGGGCAACGCGATGGGGCCGGCCTTCTGGTCGGAGATGCCGGAGGTGTTCGCAGAGCTAGATACCAACCGTG
AGGTACGGGCCGTCGTCATCACCGGATCAGGAAAAGATTTTAGCTACGGCCTGGACGTGCCCGCGATGGGCGGAAAGTTC
GTCCCATTGCTGACCGACGGCGCACTGGCACGCCCCCGCACCGACTTCCACGCCGAAGTGCTGCGGATGCAGAAGGCGAT
AAACGCCGTCGCTGATTGCCGCACGCCCACGATCGCGGCGGTGCACGGCTGGTGCATTGGTGGCGCTTTAGACCTGATCT
CCGCGGTCGATATCCGCTACGCCAGCGCCGACGCCAAGTTCTCAATGCGGGAAGTCAAACTGGCCATAGTCGCCGACATG
GGCAGTCTCGCCCGCCTTCCACTGATCTTGAGCGACGGGCATCTGCGTGAACTGGCGTTGACGGGAAAAGACATCGACGC
GACCCGCGCCGAGAAAATTGGCCTTGTAAATGATGTCTACGACAATGCCGACAAGACGCTGGCTGCCGCCCATGCCACCG
CTGCCGAGATCGCCGCCAACCCGCCACTAGCGGTCTACGGCGTCAAGGATGTCCTTTACCAACAACGCACCTCCGCGATC
TCAGAGAGCCTACGCTACGTCGCTGCGTGGAACGCGGCCTTTCTGCCGTCCAAGGATCTGACCGAGGGCATATCGGCCAC
GTTCGCCAAGCGGGCACCGCAGTTCATCGGGGAGTAG

Upstream 100 bases:

>100_bases
TGGTACGCGCCCTTTCCCTGCGTGGACACGCCAAGCAGGTGGAGACCTTCGTCCTTACCTCGTTAACCTCGGCAGACCGC
TGCCGCGCTATGGTCTTGAT

Downstream 100 bases:

>100_bases
GACACCCACTTTGACTTCAGACGGCAAGATCCGCGTCCCAACCGACCTCGACGCCGTAACGGCAATTGGTGACGAGGACC
ATTCCGACATCGACAGCGCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MGSDINRAYESVAVEIKDRVAQVTLIGPGKGNAMGPAFWSEMPEVFAELDTNREVRAVVITGSGKDFSYGLDVPAMGGKF
VPLLTDGALARPRTDFHAEVLRMQKAINAVADCRTPTIAAVHGWCIGGALDLISAVDIRYASADAKFSMREVKLAIVADM
GSLARLPLILSDGHLRELALTGKDIDATRAEKIGLVNDVYDNADKTLAAAHATAAEIAANPPLAVYGVKDVLYQQRTSAI
SESLRYVAAWNAAFLPSKDLTEGISATFAKRAPQFIGE

Sequences:

>Translated_278_residues
MGSDINRAYESVAVEIKDRVAQVTLIGPGKGNAMGPAFWSEMPEVFAELDTNREVRAVVITGSGKDFSYGLDVPAMGGKF
VPLLTDGALARPRTDFHAEVLRMQKAINAVADCRTPTIAAVHGWCIGGALDLISAVDIRYASADAKFSMREVKLAIVADM
GSLARLPLILSDGHLRELALTGKDIDATRAEKIGLVNDVYDNADKTLAAAHATAAEIAANPPLAVYGVKDVLYQQRTSAI
SESLRYVAAWNAAFLPSKDLTEGISATFAKRAPQFIGE
>Mature_277_residues
GSDINRAYESVAVEIKDRVAQVTLIGPGKGNAMGPAFWSEMPEVFAELDTNREVRAVVITGSGKDFSYGLDVPAMGGKFV
PLLTDGALARPRTDFHAEVLRMQKAINAVADCRTPTIAAVHGWCIGGALDLISAVDIRYASADAKFSMREVKLAIVADMG
SLARLPLILSDGHLRELALTGKDIDATRAEKIGLVNDVYDNADKTLAAAHATAAEIAANPPLAVYGVKDVLYQQRTSAIS
ESLRYVAAWNAAFLPSKDLTEGISATFAKRAPQFIGE

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=269, Percent_Identity=39.4052044609665, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI194097323, Length=270, Percent_Identity=24.0740740740741, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4502327, Length=182, Percent_Identity=32.4175824175824, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI157694516, Length=225, Percent_Identity=23.1111111111111, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI213417737, Length=225, Percent_Identity=23.1111111111111, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI221142681, Length=273, Percent_Identity=30.03663003663, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=29.1666666666667, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788682, Length=212, Percent_Identity=31.1320754716981, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082183, Length=283, Percent_Identity=28.2685512367491, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790281, Length=198, Percent_Identity=26.7676767676768, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787660, Length=273, Percent_Identity=27.1062271062271, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788597, Length=276, Percent_Identity=25.7246376811594, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17534483, Length=265, Percent_Identity=33.2075471698113, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17536985, Length=277, Percent_Identity=31.7689530685921, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17554946, Length=273, Percent_Identity=29.3040293040293, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=27.6923076923077, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=199, Percent_Identity=28.643216080402, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=257, Percent_Identity=25.2918287937743, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=182, Percent_Identity=29.6703296703297, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144276, Length=246, Percent_Identity=27.6422764227642, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=246, Percent_Identity=27.6422764227642, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=246, Percent_Identity=27.6422764227642, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19920382, Length=265, Percent_Identity=34.7169811320755, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI20129971, Length=279, Percent_Identity=29.0322580645161, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653477, Length=279, Percent_Identity=29.0322580645161, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653139, Length=188, Percent_Identity=31.9148936170213, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29649; Mature: 29518

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSDINRAYESVAVEIKDRVAQVTLIGPGKGNAMGPAFWSEMPEVFAELDTNREVRAVVI
CCCHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
TGSGKDFSYGLDVPAMGGKFVPLLTDGALARPRTDFHAEVLRMQKAINAVADCRTPTIAA
EECCCCCEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEH
VHGWCIGGALDLISAVDIRYASADAKFSMREVKLAIVADMGSLARLPLILSDGHLRELAL
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHEEEEEEECCCCHHHCCEEECCCCEEEEEE
TGKDIDATRAEKIGLVNDVYDNADKTLAAAHATAAEIAANPPLAVYGVKDVLYQQRTSAI
CCCCCCCHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
SESLRYVAAWNAAFLPSKDLTEGISATFAKRAPQFIGE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
GSDINRAYESVAVEIKDRVAQVTLIGPGKGNAMGPAFWSEMPEVFAELDTNREVRAVVI
CCHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
TGSGKDFSYGLDVPAMGGKFVPLLTDGALARPRTDFHAEVLRMQKAINAVADCRTPTIAA
EECCCCCEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEH
VHGWCIGGALDLISAVDIRYASADAKFSMREVKLAIVADMGSLARLPLILSDGHLRELAL
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHEEEEEEECCCCHHHCCEEECCCCEEEEEE
TGKDIDATRAEKIGLVNDVYDNADKTLAAAHATAAEIAANPPLAVYGVKDVLYQQRTSAI
CCCCCCCHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
SESLRYVAAWNAAFLPSKDLTEGISATFAKRAPQFIGE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]