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The map label for this gene is rfbE [H]

Identifier: 221229425

GI number: 221229425

Start: 154603

End: 155421

Strand: Reverse

Name: rfbE [H]

Synonym: MLBr_00114

Alternate gene names: 221229425

Gene position: 155421-154603 (Counterclockwise)

Preceding gene: 221229426

Following gene: 221229424

Centisome position: 4.76

GC content: 59.34

Gene sequence:

>819_bases
ATGGTGGAGGGTCCTCATATCGAGACAAACAATGCGTGGGTGGAATTTCCCATTTTCGACGCCAAGGCGCGTTCGTTGAA
AAAAGCCGTTCTGGGTAAGGCGGGCGGCACCATTGGGCGCAACAACTCCAACGTCGTCGTCATCGAGGCATTGCGGGACA
TCACGATGTCGCTAAGCGTAGGTGACCGAGTGGGTTTGGTTGGGCACAACGGTGCCGGGAAATCGACGCTGCTGCGCCTG
CTTTCGGGCATCTACGAGCCCACCCGAGGTTGGGCGAAGGTCACCGGCCGAGTAGCACCGGTTTTCGACCTCGGGATTGG
CATGGACCCCGAGATCTCCGGCTACGAGAACATCATAATCCGCGGCCTGTTCCTCGGGCAGACCCGAAAGCAGATGCTGG
GCAAGGTGGACGAGATCGCTGAGTTCACTGAGCTCGGCGATTACCTGTCGATGCCGCTACGCACGTATTCCACCGGGATG
CGAGTCAGACTAGCGATGGGCGTGGTCACTAGCATCGACCCCGAGATCCTGTTACTCGATGAAGGGATCGGCTCGGTGGA
CACCGACTTCCTGAAGAAAGCACAATCCCGGCTGCAGAGCTTGGTGGAACGCTCTGGAATCTTGGTCTTCGCTAGCCACT
CCAACGAGTTCCTGGCCCGATTGTGCAAGACCGCGATGTGGATCGACCACGGAGTCATCGAGCTCAGCGGCGGCATCGAA
GACGTAGTGCGTGCCTACGAAGGCGAGGACGCAGCCCGGCATGTGCGCGAGGTGATAGCCGAAACTGCCTCCGCGCAAAA
CGCTTCCGGAGATGAGTGA

Upstream 100 bases:

>100_bases
GCAAATGCGCCACAGCGCATTGCCACCGGGCATGGGCAAACCAGGACAAGCCGGCGGTCAAGGTACTGGACAGTACCTGT
AAGCCGATTGAAAGTCGTTC

Downstream 100 bases:

>100_bases
ATCCGTCTGCGCCGTCGTGGTTACCCACCGGCGTCCCGGCGAGCTAGCCAAGGCGTTGGATGTGCTGAGCACCCAGACCC
GGTTACCTGATCACTTGATC

Product: putative ABC transporter ATP-binding component

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MVEGPHIETNNAWVEFPIFDAKARSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMSLSVGDRVGLVGHNGAGKSTLLRL
LSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENIIIRGLFLGQTRKQMLGKVDEIAEFTELGDYLSMPLRTYSTGM
RVRLAMGVVTSIDPEILLLDEGIGSVDTDFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGVIELSGGIE
DVVRAYEGEDAARHVREVIAETASAQNASGDE

Sequences:

>Translated_272_residues
MVEGPHIETNNAWVEFPIFDAKARSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMSLSVGDRVGLVGHNGAGKSTLLRL
LSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENIIIRGLFLGQTRKQMLGKVDEIAEFTELGDYLSMPLRTYSTGM
RVRLAMGVVTSIDPEILLLDEGIGSVDTDFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGVIELSGGIE
DVVRAYEGEDAARHVREVIAETASAQNASGDE
>Mature_272_residues
MVEGPHIETNNAWVEFPIFDAKARSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMSLSVGDRVGLVGHNGAGKSTLLRL
LSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENIIIRGLFLGQTRKQMLGKVDEIAEFTELGDYLSMPLRTYSTGM
RVRLAMGVVTSIDPEILLLDEGIGSVDTDFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGVIELSGGIE
DVVRAYEGEDAARHVREVIAETASAQNASGDE

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbD [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=222, Percent_Identity=24.3243243243243, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788761, Length=235, Percent_Identity=26.8085106382979, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789751, Length=235, Percent_Identity=26.3829787234043, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082267, Length=201, Percent_Identity=28.8557213930348, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790467, Length=192, Percent_Identity=27.6041666666667, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787041, Length=243, Percent_Identity=26.3374485596708, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081791, Length=180, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48995001, Length=182, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081782, Length=215, Percent_Identity=28.8372093023256, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081709, Length=217, Percent_Identity=23.5023041474654, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787089, Length=207, Percent_Identity=25.1207729468599, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786319, Length=200, Percent_Identity=25.5, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788450, Length=225, Percent_Identity=25.7777777777778, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17565586, Length=213, Percent_Identity=26.7605633802817, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=210, Percent_Identity=28.0952380952381, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 29458; Mature: 29458

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEGPHIETNNAWVEFPIFDAKARSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMSLSV
CCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHEEEC
GDRVGLVGHNGAGKSTLLRLLSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENIII
CCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCEECCCCCCCCCCCCCCEEE
RGLFLGQTRKQMLGKVDEIAEFTELGDYLSMPLRTYSTGMRVRLAMGVVTSIDPEILLLD
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHCCCCCEEEEE
EGIGSVDTDFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGVIELSGGIE
CCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCHH
DVVRAYEGEDAARHVREVIAETASAQNASGDE
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVEGPHIETNNAWVEFPIFDAKARSLKKAVLGKAGGTIGRNNSNVVVIEALRDITMSLSV
CCCCCEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHEEEC
GDRVGLVGHNGAGKSTLLRLLSGIYEPTRGWAKVTGRVAPVFDLGIGMDPEISGYENIII
CCEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCEECCCCCCCCCCCCCCEEE
RGLFLGQTRKQMLGKVDEIAEFTELGDYLSMPLRTYSTGMRVRLAMGVVTSIDPEILLLD
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHCCCCCEEEEE
EGIGSVDTDFLKKAQSRLQSLVERSGILVFASHSNEFLARLCKTAMWIDHGVIELSGGIE
CCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCHH
DVVRAYEGEDAARHVREVIAETASAQNASGDE
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7692217 [H]