Click here to switch to the map view.

The map label for this gene is fadD32 [H]

Identifier: 221229413

GI number: 221229413

Start: 124626

End: 126533

Strand: Direct

Name: fadD32 [H]

Synonym: MLBr_00100

Alternate gene names: 221229413

Gene position: 124626-126533 (Clockwise)

Preceding gene: 221229412

Following gene: 221229414

Centisome position: 3.81

GC content: 61.37

Gene sequence:

>1908_bases
GTGACAGGAGAGGGTCGCATGGCGTACCACAACCCGTTCATTGTGAATGGAAAGATCAGGTTCCCGCCTAATACCAACCT
GGTCCGTCACGTCGAAAAGTGGGCAAAGGTTCGCGGCGACAAGTTGGCTTATCGATTCCTGGACTTCTCCACCGGCCGCG
ATGGTGTCGCGCGCGACATTTTGTGGTCCGAATTCAGCGCCCGCAACCGAGCGGTGGGCGCCCGCTTACAACAAGTCACC
CAGCCGGGTGACCGCATCGCCATCCTATGTCCGCAGAACCTGGACTACCTGATCTCCTTCTTCGGTGCCCTGTATTCCGG
CCGGATCGCGGTGCCGTTGTTCGACCCGGCCGAGCCGGGGCATGTCGGCCGGTTACACGCAGTGCTCGACGATTGCACTC
CCTCGACTATCCTAACTACCACCGACTCCGCCGAAGGTGTCCGCAAGTTCATCCGGGGTCGGTTTGCTAAGGAGCGAACT
CGCGTTATCGCGGTTGACGCAGTGCCCAACGAGGTTGCCGCCACCTGGCAGCAGCCTGAGGCAAACGAGGAAACCGTGGC
CTATCTGCAGTACACGTCGGGCTCCACCCGCATTCCGAGCGGCGTGCAGATCACCCACCTGAACCTACCCACCAATGTAT
TGCAGGTGCTGAATTCCCTGCAAGGACAGGAAGGCGACCGTGGGGTCAGCTGGCTTCCGTTCTTCCACGACATGGGTCTT
ATTACGGTGCTGCTCCCGTCGGTTCTTGGTCACAGCTTTACCTTTATGACGCCCGCGGCGTTCGTGCGTCGGCCCGGTCG
CTGGATTCGCGAACTTGCGCGTAAGCCCGAAGACACCGGCGGCACCTTCTCGGCGGCGCCGAACTTCGCGTTTGAACACG
CCGCGATGCGTGGACTGCCTCGAGATGACGAGTTGCCACTGGATCTGAGTAACGTCAAAGGCATCCTCAACGGCAGCGAG
CCGGTGTCACCGGCGTCGATGCGCAAGTTTTTCGAAGCGTTTGCGCCGTACGGTTTGAAGCAGACCGCCGTCAAGCCGTC
CTACGGGCTAGCCGAAGCTACACTGTTTGTCTCGACCACCCCGATGGACGAGTCGCCCACGGTGATCCACGTGGACCGCT
ACGAGCTGAACAATCAGCGATTCGTGGAGGTGCCCGCTGATGCACCGAACGCGGTAGCCCAGGTTTCCGCGGGCAAGGTC
GGGGTGAGCGAATGGGCGATCATCGTCGATGCCGACACTGCCAGTGAGTTACCAGACGGGCAGATCGGTGAGATCTGGCT
GCACGGCAACAACCTCGGTACCGGTTATTGGGGCAAGGAAGAAGAGTCCGCCCAGACCTTCAACAACATCCTCAAATCTC
GGATCAGTGAGTCGCGTGCCGAAGGAGCCTCCGACGACGCGTCGTGGGTGCGTACCGGAGACTACGGCACCTACTTCAAG
GACCATCTCTATATAGCCGGCCGGATCAAGGACCTCGTCATCATCGATGGCCGCAACCATTACCCGCAGGACCTGGAATA
CACGGCACAGGAATCGACTAAGGCGTTGCGCGTTGGCTACGTAGCCGCCTTCTCCGTCCCCGCCAACCAATTGGCCCAGG
TGGTGCTCGAGGACCCGCACGTCGGCCTAAAGTTCGATGCCGAGGACACCTCCGAGCAGTTGGTGATCGTCGGGGAGCGC
GCTGCTGGCACGCACAAGCTTGACTACCAGCCCATCGTTGACGACATCCGCGCGGCTATCGCTGTGAGGCATGGGGTGGC
GGTGCGTGATGTGCTGCTGGTGCCGGCCGGAACGATTCCCCGCACTTCCAGCGGCAAGATCGGCCGGCGCGCCTGCCGCG
CCGCCTACATTGACGGTAGTCTGCGCAGCGGCGCCAGCTCTCCGACGGTTTTCGCTGCTGAATTCTGA

Upstream 100 bases:

>100_bases
CGAAGCTAGCAGTGGCCAGCGCACGCCTAAATGTGCGTCGTTCGAAGGTTGGCCCGGGTGCAGGTTGGAACTGTCGGCGA
AGCCGACCGAGAGATGTGTC

Downstream 100 bases:

>100_bases
GCAAGGCACACATGGCTGACGTAGACGCACCCCAATTGAACCAGGACCCTCCCCAATTGAACCAGGACCCTCAAGCGAAC
CTCCCCGCTGAGAAGACCAA

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 635; Mature: 634

Protein sequence:

>635_residues
MTGEGRMAYHNPFIVNGKIRFPPNTNLVRHVEKWAKVRGDKLAYRFLDFSTGRDGVARDILWSEFSARNRAVGARLQQVT
QPGDRIAILCPQNLDYLISFFGALYSGRIAVPLFDPAEPGHVGRLHAVLDDCTPSTILTTTDSAEGVRKFIRGRFAKERT
RVIAVDAVPNEVAATWQQPEANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVLQVLNSLQGQEGDRGVSWLPFFHDMGL
ITVLLPSVLGHSFTFMTPAAFVRRPGRWIRELARKPEDTGGTFSAAPNFAFEHAAMRGLPRDDELPLDLSNVKGILNGSE
PVSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVSTTPMDESPTVIHVDRYELNNQRFVEVPADAPNAVAQVSAGKV
GVSEWAIIVDADTASELPDGQIGEIWLHGNNLGTGYWGKEEESAQTFNNILKSRISESRAEGASDDASWVRTGDYGTYFK
DHLYIAGRIKDLVIIDGRNHYPQDLEYTAQESTKALRVGYVAAFSVPANQLAQVVLEDPHVGLKFDAEDTSEQLVIVGER
AAGTHKLDYQPIVDDIRAAIAVRHGVAVRDVLLVPAGTIPRTSSGKIGRRACRAAYIDGSLRSGASSPTVFAAEF

Sequences:

>Translated_635_residues
MTGEGRMAYHNPFIVNGKIRFPPNTNLVRHVEKWAKVRGDKLAYRFLDFSTGRDGVARDILWSEFSARNRAVGARLQQVT
QPGDRIAILCPQNLDYLISFFGALYSGRIAVPLFDPAEPGHVGRLHAVLDDCTPSTILTTTDSAEGVRKFIRGRFAKERT
RVIAVDAVPNEVAATWQQPEANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVLQVLNSLQGQEGDRGVSWLPFFHDMGL
ITVLLPSVLGHSFTFMTPAAFVRRPGRWIRELARKPEDTGGTFSAAPNFAFEHAAMRGLPRDDELPLDLSNVKGILNGSE
PVSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVSTTPMDESPTVIHVDRYELNNQRFVEVPADAPNAVAQVSAGKV
GVSEWAIIVDADTASELPDGQIGEIWLHGNNLGTGYWGKEEESAQTFNNILKSRISESRAEGASDDASWVRTGDYGTYFK
DHLYIAGRIKDLVIIDGRNHYPQDLEYTAQESTKALRVGYVAAFSVPANQLAQVVLEDPHVGLKFDAEDTSEQLVIVGER
AAGTHKLDYQPIVDDIRAAIAVRHGVAVRDVLLVPAGTIPRTSSGKIGRRACRAAYIDGSLRSGASSPTVFAAEF
>Mature_634_residues
TGEGRMAYHNPFIVNGKIRFPPNTNLVRHVEKWAKVRGDKLAYRFLDFSTGRDGVARDILWSEFSARNRAVGARLQQVTQ
PGDRIAILCPQNLDYLISFFGALYSGRIAVPLFDPAEPGHVGRLHAVLDDCTPSTILTTTDSAEGVRKFIRGRFAKERTR
VIAVDAVPNEVAATWQQPEANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVLQVLNSLQGQEGDRGVSWLPFFHDMGLI
TVLLPSVLGHSFTFMTPAAFVRRPGRWIRELARKPEDTGGTFSAAPNFAFEHAAMRGLPRDDELPLDLSNVKGILNGSEP
VSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVSTTPMDESPTVIHVDRYELNNQRFVEVPADAPNAVAQVSAGKVG
VSEWAIIVDADTASELPDGQIGEIWLHGNNLGTGYWGKEEESAQTFNNILKSRISESRAEGASDDASWVRTGDYGTYFKD
HLYIAGRIKDLVIIDGRNHYPQDLEYTAQESTKALRVGYVAAFSVPANQLAQVVLEDPHVGLKFDAEDTSEQLVIVGERA
AGTHKLDYQPIVDDIRAAIAVRHGVAVRDVLLVPAGTIPRTSSGKIGRRACRAAYIDGSLRSGASSPTVFAAEF

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=580, Percent_Identity=23.2758620689655, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI55749758, Length=621, Percent_Identity=23.6714975845411, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI45827692, Length=493, Percent_Identity=22.5152129817444, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI225735629, Length=493, Percent_Identity=22.5152129817444, Blast_Score=95, Evalue=3e-19,
Organism=Homo sapiens, GI225735625, Length=627, Percent_Identity=21.5311004784689, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI45827694, Length=542, Percent_Identity=21.2177121771218, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1788107, Length=594, Percent_Identity=25.2525252525253, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI193202679, Length=583, Percent_Identity=22.8130360205832, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654656, Length=444, Percent_Identity=23.8738738738739, Blast_Score=95, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 69535; Mature: 69404

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGEGRMAYHNPFIVNGKIRFPPNTNLVRHVEKWAKVRGDKLAYRFLDFSTGRDGVARDI
CCCCCCEEECCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
LWSEFSARNRAVGARLQQVTQPGDRIAILCPQNLDYLISFFGALYSGRIAVPLFDPAEPG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
HVGRLHAVLDDCTPSTILTTTDSAEGVRKFIRGRFAKERTRVIAVDAVPNEVAATWQQPE
CCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCC
ANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVLQVLNSLQGQEGDRGVSWLPFFHDMGL
CCCCEEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCEEECHHHHHHH
ITVLLPSVLGHSFTFMTPAAFVRRPGRWIRELARKPEDTGGTFSAAPNFAFEHAAMRGLP
HHHHHHHHHCCCEECCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCC
RDDELPLDLSNVKGILNGSEPVSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVSTT
CCCCCCCCHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEEEEEEC
PMDESPTVIHVDRYELNNQRFVEVPADAPNAVAQVSAGKVGVSEWAIIVDADTASELPDG
CCCCCCCEEEEEEEECCCCEEEECCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCC
QIGEIWLHGNNLGTGYWGKEEESAQTFNNILKSRISESRAEGASDDASWVRTGDYGTYFK
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEE
DHLYIAGRIKDLVIIDGRNHYPQDLEYTAQESTKALRVGYVAAFSVPANQLAQVVLEDPH
CEEEEEEEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHCCCC
VGLKFDAEDTSEQLVIVGERAAGTHKLDYQPIVDDIRAAIAVRHGVAVRDVLLVPAGTIP
CCEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
RTSSGKIGRRACRAAYIDGSLRSGASSPTVFAAEF
CCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECC
>Mature Secondary Structure 
TGEGRMAYHNPFIVNGKIRFPPNTNLVRHVEKWAKVRGDKLAYRFLDFSTGRDGVARDI
CCCCCEEECCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHH
LWSEFSARNRAVGARLQQVTQPGDRIAILCPQNLDYLISFFGALYSGRIAVPLFDPAEPG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCC
HVGRLHAVLDDCTPSTILTTTDSAEGVRKFIRGRFAKERTRVIAVDAVPNEVAATWQQPE
CCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCCCC
ANEETVAYLQYTSGSTRIPSGVQITHLNLPTNVLQVLNSLQGQEGDRGVSWLPFFHDMGL
CCCCEEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCEEECHHHHHHH
ITVLLPSVLGHSFTFMTPAAFVRRPGRWIRELARKPEDTGGTFSAAPNFAFEHAAMRGLP
HHHHHHHHHCCCEECCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCC
RDDELPLDLSNVKGILNGSEPVSPASMRKFFEAFAPYGLKQTAVKPSYGLAEATLFVSTT
CCCCCCCCHHHCCCEECCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEEEEEEC
PMDESPTVIHVDRYELNNQRFVEVPADAPNAVAQVSAGKVGVSEWAIIVDADTASELPDG
CCCCCCCEEEEEEEECCCCEEEECCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCCCC
QIGEIWLHGNNLGTGYWGKEEESAQTFNNILKSRISESRAEGASDDASWVRTGDYGTYFK
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEE
DHLYIAGRIKDLVIIDGRNHYPQDLEYTAQESTKALRVGYVAAFSVPANQLAQVVLEDPH
CEEEEEEEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHCCCC
VGLKFDAEDTSEQLVIVGERAAGTHKLDYQPIVDDIRAAIAVRHGVAVRDVLLVPAGTIP
CCEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
RTSSGKIGRRACRAAYIDGSLRSGASSPTVFAAEF
CCCCCCHHHHHHHHHHCCCHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]